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<title>UTas ePrints - Hormonally Induced Spawning, Embryonic Development and Larval Rearing of the Southern Temperate Banded Morwong Cheilodactylus spectabilis</title>
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<meta content="Ritar, Arthur J." name="eprints.creators_name" />
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<meta content="Banded morwong (Cheilodactylus spectabilis) are of interest for marine finfish aquaculture in temperate southern Australia. To improve their ovulatory response, adult females were implanted during the autumn spawning season with slow-release pellets containing 0-400 ug luteinizing hormone releasing hormone analogue (LHRHa)/kg body weight within 24 h of capture from the wild. Compared to the sham control group, animals treated with LHRHa produced significantly more eggs on each day after implantation for the following 7 days (91 +/- 39 ml and 290 +/- 38 ml) and a higher proportion ovulated (8/12 and 27/27). Of fish treated with LHRHa, 93% ovulated two days after implantation and 79% ovulated three times at two-day intervals, whereas control animals showed no cyclicity of ovulation and few ovulated more than once. Egg production was highest at the first ovulation after LHRHa treatment and declined at subsequent ovulations. In a second experiment investigating the range 100-400 ug LHRHa, there was no effect of dose rate on ovulation parameters which additionally examined implantation either immediately after capture or after a 5-day delay. Compared to immediate implantation, a delay resulted in a lower proportion of animals which could be stripped after implantation (100% and 50%, respectively) and the volume of eggs was lower (135 +/- 15 ml and 107 +/- 10 ml). The egg quality was poor following delayed implantation, resulting in no fertilisation after artificial insemination compared with immediate implantation in which fertilisation and hatch rates were higher for eggs collected on Day 2 after implantation (79 +/- 8% and 58 +/- 9%) than on Day 4 (23 +/- 7% and 15 +/- 6%). Thus, it is important to implant animals as soon as possible after capture to ensure optimum egg quality. Good quality eggs were buoyant, spherical and had a diameter of 1050 +/- 25 um with a single pigmented oil droplet of 190 +/- 9 um. When a separate large batch of eggs collected two days after implantation with 100 ug LHRHa was inseminated and cultured at 18 C, larvae hatched after 63 +/- 2 h at a standard length of 2.6 +/- 0.4 mm. Newly-hatched larvae were buoyant and transparent with only a few melanophores, eyes were non-pigmented and jaws were non-funtional. By the fourth day, jaws were functional and eyes were fully pigmented. Utilisation of the endogenous yolk and oil was completed by Day 6 and swimming commenced with exogenous feeding. Larvae, initially fed lipid-enriched rotifers followed by Artemia, reached 8.9 +/- 0.7 mm length on Day 55, after which they metamorphosed to the post-larval paperfish stage of development, 22 +/- 0.9 mm on Day 100, and 43 +/- 1.0 mm at six months old. The results show that treatment of wild-caught females with slow-release pellets containing LHRHa is effective for the production of eggs for hatchery rearing." name="eprints.abstract" />
<meta content="2006-12" name="eprints.date" />
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<meta content="Journal of the World Aquaculture Society" name="eprints.publication" />
<meta content="37" name="eprints.volume" />
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<meta content="397-406" name="eprints.pagerange" />
<meta content="10.1111/j.1749-7345.2006.00053.x" name="eprints.id_number" />
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<meta content="Battaglene, S.C. and J.M. Cobcroft. (in review). Advances in the culture of striped trumpeter larvae: a review. Aquaculture
Bromage, N. 1995. Broodstock management and seed quality - general considerations. Pages 1-24 in N.R. Bromage and R.J. Roberts, editors. Broodstock Management and Egg and Larval Quality. Blackwell Science, Oxford, U.K..
Bruce, B. 1989. Cheilodactylidae: Morwongs. Pages 150-153 in J.M. Leis and T. Trnski, editors. The Larvae of Indo-Pacific Shorefishes. New South Wales University Press, Sydney, Australia.
Bruce, B. 1998. Cheilodactylidae: Morwongs. Pages 210-213 in F.J. Neira, A.G. Miskiewicz and T. Trnski, editors. Larvae of Temperate Australian Fishes: Laboratory Guide for Larval Fish Identification. University of Western Auastralia Press, Nedlands, Australia.
Clearwater, S.J. and N.W. Pankhurst. 1997. The response to capture and confinement stress of plasma cortisol, plasma sex steroids and vitellogenic oocytes in the marine teleost, red gurnard. Journal of Fish Biology 50:429-441.
Crim, L.W., R.E. Peter, and G. Van Der Kraak. 1987. The use of LHRH analogs in aquaculture. Pages 489-498 in B.C. Vickery and J.J., Jr. Nestor, editors. LHRH and its Analogs: Contraceptive and Therapeutic Application, Part II. MTP Press, Lancaster, U.K..
Jordan, A. 1994. Species profile - banded morwong. Fishing Today 7(4):35-37.
Kuiter, R.H. 1993. Coastal fishes of South-Eastern Australia. University of Hawai'i Press, U.S.A.
Last, P.R., E.O.G. Scott, and F.H. Talbot. 1983. Fishes of Tasmania. Tasmanian Fisheries Development Authority, Hobart, Tasmania, Australia.
Lee, C.-S., C.S. Tamaru and C.D. Kelley. 1986. Technique for making chronic-release LHRHa and 17alpha-methyl-testosterone pellets for intramuscular implantation in fishes. Aquaculture 59:161-168.
Leum, L.L. and J.H. Choat 1980. Density and distribution patterns of the temperate marine fish Cheilodactylus spectabilis (Cheilodactylidae) in a reef environment. Marine Biology 57:327-337.
McCormick, M.I. 1989. Reproductive ecology of the temperate reef fish Cheilodactylus spectabilis (Pisces: Cheilodactylidae). Marine Ecology Progress Series 55:113-120.
Morehead, D. T., N.W. Pankhurst and A.J. Ritar. 1998. Effect of treatment with LHRH analogue on oocyte maturation, plasma sex steroid levels and egg production in female striped trumpeter Latris lineata (Latrididae). Aquaculture 169:315-331.
Murphy, R.J., and Lyle, J.M. 1999. Impact of gillnet fishing on inshore temperate reef fishes, with particular reference to banded morwong. University of Tasmania, Marine Research Laboratories, Tasmanian Aquaculture and Fisheries Institute, Report for the Fisheries Research Development Corporation, Final Report 95/145. 136 pp. http://www.utas.edu.au/tafi/PDF_files/GillnetFRDC-finalreport.pdf.
Mylonas, C.C. and Y. Zohar. 2001. Use of GnRHa-delivery systems for the control of reproduction in fish. Reviews in Fish Biology and Fisheries 10:463-491.
Pankhurst, N.W. 1998. Reproduction. Pages 1-26 in Black, K.D., Pickering, A.D., editors. Biology of Farmed Fish. Sheffield Academic Press, Sheffield, U.K..
Ritar, A.J. 1999. Artificial insemination with cryopreserved semen from striped trumpeter (Latris lineata). Aquaculture 180:177-187.
Roberts, C.D. 1995. A review of postlarval paperfish stage and its contribution to Cheilodactylid systematics. International Larval Fish Conference, Sydney, Australia.
Searle, L.D. and W. Zacharin. 1994. Aquaculture research needs in Tasmania: species priorities and research strategies for the development of finfish, invertebrate and seaweed industries. Internal Report. Department of Primary Industry and Fisheries, Tasmania, Australia.
Steel, R.G.D. and J.H. Torrie. 1960. Principles and Procedures of Statistics. McGraw-Hill, New York, U.S.A.
Trotter, A.J., P.M. Pankhurst and S.C. Battaglene. 2004. Morphological development of the swim bladder in hatchery-reared striped trumpeter Latris lineata. Journal of Applied Ichthyology 20:395-401.
Wolf, B. 1998. Update on juvenile banded morwong in Tasmania. Fishing Today 11(4):30-31.
Woods, L.C. and C.V. Sullivan. 1993. Reproduction of striped bass, Morone saxatilis (Walbaum), broodstock: monitoring maturation and hormonal induction of spawning. Aquaculture and Fisheries Management 24:211-222.
Ziegler, P.E., Lyle, J., Haddon, M., Burch, P. 2005. Developing assessments and performance indicators for a small-scale temperate reef fish fishery. In: Kruse, G.H., Gallucci, V.F., Hay, D.E., Perry, R.I., Peterman, R.M., Shirley, T.C., Spencer, P.D., Wilson, B., and Woodby, D. (eds.). Assessment and management of new and developed fisheries in data-limited situations. Alaska Sea Grants College Program, University of Alaska Fairbanks, Alaska, 195-211.
Zohar, Y. 1986. Gonadotropin releasing hormone in spawning induction in teleosts: basic and applied consideration. Pages 47-61 in Zohar, Y. and Breton, B., editors. Reproduction in Fish - Basic and Applied Aspects in Endocrinology and Genetics. INRA, Paris, France.
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<meta content="Ritar, Arthur J. and Pribadi, Tyas A. (2006) Hormonally Induced Spawning, Embryonic Development and Larval Rearing of the Southern Temperate Banded Morwong Cheilodactylus spectabilis. Journal of the World Aquaculture Society, 37 (4). pp. 397-406." name="eprints.citation" />
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<meta content="Banded morwong (Cheilodactylus spectabilis) are of interest for marine finfish aquaculture in temperate southern Australia. To improve their ovulatory response, adult females were implanted during the autumn spawning season with slow-release pellets containing 0-400 ug luteinizing hormone releasing hormone analogue (LHRHa)/kg body weight within 24 h of capture from the wild. Compared to the sham control group, animals treated with LHRHa produced significantly more eggs on each day after implantation for the following 7 days (91 +/- 39 ml and 290 +/- 38 ml) and a higher proportion ovulated (8/12 and 27/27). Of fish treated with LHRHa, 93% ovulated two days after implantation and 79% ovulated three times at two-day intervals, whereas control animals showed no cyclicity of ovulation and few ovulated more than once. Egg production was highest at the first ovulation after LHRHa treatment and declined at subsequent ovulations. In a second experiment investigating the range 100-400 ug LHRHa, there was no effect of dose rate on ovulation parameters which additionally examined implantation either immediately after capture or after a 5-day delay. Compared to immediate implantation, a delay resulted in a lower proportion of animals which could be stripped after implantation (100% and 50%, respectively) and the volume of eggs was lower (135 +/- 15 ml and 107 +/- 10 ml). The egg quality was poor following delayed implantation, resulting in no fertilisation after artificial insemination compared with immediate implantation in which fertilisation and hatch rates were higher for eggs collected on Day 2 after implantation (79 +/- 8% and 58 +/- 9%) than on Day 4 (23 +/- 7% and 15 +/- 6%). Thus, it is important to implant animals as soon as possible after capture to ensure optimum egg quality. Good quality eggs were buoyant, spherical and had a diameter of 1050 +/- 25 um with a single pigmented oil droplet of 190 +/- 9 um. When a separate large batch of eggs collected two days after implantation with 100 ug LHRHa was inseminated and cultured at 18 C, larvae hatched after 63 +/- 2 h at a standard length of 2.6 +/- 0.4 mm. Newly-hatched larvae were buoyant and transparent with only a few melanophores, eyes were non-pigmented and jaws were non-funtional. By the fourth day, jaws were functional and eyes were fully pigmented. Utilisation of the endogenous yolk and oil was completed by Day 6 and swimming commenced with exogenous feeding. Larvae, initially fed lipid-enriched rotifers followed by Artemia, reached 8.9 +/- 0.7 mm length on Day 55, after which they metamorphosed to the post-larval paperfish stage of development, 22 +/- 0.9 mm on Day 100, and 43 +/- 1.0 mm at six months old. The results show that treatment of wild-caught females with slow-release pellets containing LHRHa is effective for the production of eggs for hatchery rearing." name="DC.description" />
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<h1 class="ep_tm_pagetitle">Hormonally Induced Spawning, Embryonic Development and Larval Rearing of the Southern Temperate Banded Morwong Cheilodactylus spectabilis</h1>
<p style="margin-bottom: 1em" class="not_ep_block"><span class="person_name">Ritar, Arthur J.</span> and <span class="person_name">Pribadi, Tyas A.</span> (2006) <xhtml:em>Hormonally Induced Spawning, Embryonic Development and Larval Rearing of the Southern Temperate Banded Morwong Cheilodactylus spectabilis.</xhtml:em> Journal of the World Aquaculture Society, 37 (4). pp. 397-406.</p><p style="margin-bottom: 1em" class="not_ep_block"></p><table style="margin-bottom: 1em" class="not_ep_block"><tr><td valign="top" style="text-align:center"><a onmouseover="EPJS_ShowPreview( event, 'doc_preview_1883' );" href="http://eprints.utas.edu.au/1478/1/BMW_JWAS_E-print.pdf" onmouseout="EPJS_HidePreview( event, 'doc_preview_1883' );"><img alt="[img]" src="http://eprints.utas.edu.au/style/images/fileicons/application_pdf.png" class="ep_doc_icon" border="0" /></a><div class="ep_preview" id="doc_preview_1883"><table><tr><td><img alt="" src="http://eprints.utas.edu.au/1478/thumbnails/1/preview.png" class="ep_preview_image" border="0" /><div class="ep_preview_title">Preview</div></td></tr></table></div></td><td valign="top"><a href="http://eprints.utas.edu.au/1478/1/BMW_JWAS_E-print.pdf"><span class="ep_document_citation">PDF (Author version)</span></a> - Requires a PDF viewer<br />153Kb</td></tr><tr><td valign="top" style="text-align:center"><a href="http://eprints.utas.edu.au/1478/2/ritar-pubversion.pdf"><img alt="[img]" src="http://eprints.utas.edu.au/style/images/fileicons/application_pdf.png" class="ep_doc_icon" border="0" /></a></td><td valign="top"><a href="http://eprints.utas.edu.au/1478/2/ritar-pubversion.pdf"><span class="ep_document_citation">PDF</span></a> - Full text restricted - Requires a PDF viewer<br />205Kb</td></tr></table><p style="margin-bottom: 1em" class="not_ep_block">Official URL: <a href="http://dx.doi.org/10.1111/j.1749-7345.2006.00053.x">http://dx.doi.org/10.1111/j.1749-7345.2006.00053.x</a></p><div class="not_ep_block"><h2>Abstract</h2><p style="padding-bottom: 16px; text-align: left; margin: 1em auto 0em auto">Banded morwong (Cheilodactylus spectabilis) are of interest for marine finfish aquaculture in temperate southern Australia. To improve their ovulatory response, adult females were implanted during the autumn spawning season with slow-release pellets containing 0-400 ug luteinizing hormone releasing hormone analogue (LHRHa)/kg body weight within 24 h of capture from the wild. Compared to the sham control group, animals treated with LHRHa produced significantly more eggs on each day after implantation for the following 7 days (91 +/- 39 ml and 290 +/- 38 ml) and a higher proportion ovulated (8/12 and 27/27). Of fish treated with LHRHa, 93% ovulated two days after implantation and 79% ovulated three times at two-day intervals, whereas control animals showed no cyclicity of ovulation and few ovulated more than once. Egg production was highest at the first ovulation after LHRHa treatment and declined at subsequent ovulations. In a second experiment investigating the range 100-400 ug LHRHa, there was no effect of dose rate on ovulation parameters which additionally examined implantation either immediately after capture or after a 5-day delay. Compared to immediate implantation, a delay resulted in a lower proportion of animals which could be stripped after implantation (100% and 50%, respectively) and the volume of eggs was lower (135 +/- 15 ml and 107 +/- 10 ml). The egg quality was poor following delayed implantation, resulting in no fertilisation after artificial insemination compared with immediate implantation in which fertilisation and hatch rates were higher for eggs collected on Day 2 after implantation (79 +/- 8% and 58 +/- 9%) than on Day 4 (23 +/- 7% and 15 +/- 6%). Thus, it is important to implant animals as soon as possible after capture to ensure optimum egg quality. Good quality eggs were buoyant, spherical and had a diameter of 1050 +/- 25 um with a single pigmented oil droplet of 190 +/- 9 um. When a separate large batch of eggs collected two days after implantation with 100 ug LHRHa was inseminated and cultured at 18 C, larvae hatched after 63 +/- 2 h at a standard length of 2.6 +/- 0.4 mm. Newly-hatched larvae were buoyant and transparent with only a few melanophores, eyes were non-pigmented and jaws were non-funtional. By the fourth day, jaws were functional and eyes were fully pigmented. Utilisation of the endogenous yolk and oil was completed by Day 6 and swimming commenced with exogenous feeding. Larvae, initially fed lipid-enriched rotifers followed by Artemia, reached 8.9 +/- 0.7 mm length on Day 55, after which they metamorphosed to the post-larval paperfish stage of development, 22 +/- 0.9 mm on Day 100, and 43 +/- 1.0 mm at six months old. The results show that treatment of wild-caught females with slow-release pellets containing LHRHa is effective for the production of eggs for hatchery rearing.</p></div><table style="margin-bottom: 1em" cellpadding="3" class="not_ep_block" border="0"><tr><th valign="top" class="ep_row">Item Type:</th><td valign="top" class="ep_row">Article</td></tr><tr><th valign="top" class="ep_row">Additional Information:</th><td valign="top" class="ep_row">The definitive version is available at www.blackwell-synergy.com</td></tr><tr><th valign="top" class="ep_row">Keywords:</th><td valign="top" class="ep_row">Spawning, Embryonic Development, Larval Rearing, Southern Temperate Banded Morwong, Cheilodactylus spectabilis</td></tr><tr><th valign="top" class="ep_row">Subjects:</th><td valign="top" class="ep_row"><a href="http://eprints.utas.edu.au/view/subjects/300799.html">300000 Agricultural, Veterinary and Environmental Sciences > 300700 Fisheries Sciences > 300799 Fisheries Sciences not elsewhere classified</a><br /><a href="http://eprints.utas.edu.au/view/subjects/300703.html">300000 Agricultural, Veterinary and Environmental Sciences > 300700 Fisheries Sciences > 300703 Aquaculture</a></td></tr><tr><th valign="top" class="ep_row">ID Code:</th><td valign="top" class="ep_row">1478</td></tr><tr><th valign="top" class="ep_row">Deposited By:</th><td valign="top" class="ep_row"><span class="ep_name_citation"><span class="person_name">Dr Arthur J Ritar</span></span></td></tr><tr><th valign="top" class="ep_row">Deposited On:</th><td valign="top" class="ep_row">26 Jul 2007</td></tr><tr><th valign="top" class="ep_row">Last Modified:</th><td valign="top" class="ep_row">09 Jan 2008 02:30</td></tr><tr><th valign="top" class="ep_row">ePrint Statistics:</th><td valign="top" class="ep_row"><a target="ePrintStats" href="/es/index.php?action=show_detail_eprint;id=1478;">View statistics for this ePrint</a></td></tr></table><p align="right">Repository Staff Only: <a href="http://eprints.utas.edu.au/cgi/users/home?screen=EPrint::View&eprintid=1478">item control page</a></p>
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