<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" "http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd"> <html> <head> <title>UTas ePrints - Comparison of antifungal and antioxidant activities of Acacia mangium and A. auriculiformis heartwood extracts</title> <script type="text/javascript" src="http://eprints.utas.edu.au/javascript/auto.js"><!-- padder --></script> <style type="text/css" media="screen">@import url(http://eprints.utas.edu.au/style/auto.css);</style> <style type="text/css" media="print">@import url(http://eprints.utas.edu.au/style/print.css);</style> <link rel="icon" href="/images/eprints/favicon.ico" type="image/x-icon" /> <link rel="shortcut icon" href="/images/eprints/favicon.ico" type="image/x-icon" /> <link rel="Top" href="http://eprints.utas.edu.au/" /> <link rel="Search" href="http://eprints.utas.edu.au/cgi/search" /> <meta content="Mihara, R." name="eprints.creators_name" /> <meta content="Barry, K.M." name="eprints.creators_name" /> <meta content="Mohammed, C.L." name="eprints.creators_name" /> <meta content="Mitsunaga, T." name="eprints.creators_name" /> <meta content="" name="eprints.creators_id" /> <meta content="Karen.Barry@utas.edu.au" name="eprints.creators_id" /> <meta content="" name="eprints.creators_id" /> <meta content="" name="eprints.creators_id" /> <meta content="article" name="eprints.type" /> <meta content="2007-10-11 05:00:34" name="eprints.datestamp" /> <meta content="2008-01-08 15:30:00" name="eprints.lastmod" /> <meta content="show" name="eprints.metadata_visibility" /> <meta content="Comparison of antifungal and antioxidant activities of Acacia mangium and A. auriculiformis heartwood extracts" name="eprints.title" /> <meta content="pub" name="eprints.ispublished" /> <meta content="270305" name="eprints.subjects" /> <meta content="300603" name="eprints.subjects" /> <meta content="250302" name="eprints.subjects" /> <meta content="restricted" name="eprints.full_text_status" /> <meta content="Acacia mangium - A. auriculiformis - heartrot - heartwood extracts - flavanone - Phellinus noxius - P. badius - antifungal activity - antioxidant - laccase inhibition" name="eprints.keywords" /> <meta content="The original publication is available at www.springerlink.com" name="eprints.note" /> <meta content="The effect of heartwood extracts from Acacia mangium (heartrot-susceptible) and A. auriculiformis (heartrot-resistant) was examined on the growth of wood rotting fungi with in vitro assays. A. auriculiformis heartwood extracts had higher antifungal activity than A. mangium. The compounds 3,4,7,8-tetrahydroxyflavanone and teracacidin (the most abundant flavonoids in both species) showed antifungal activity. A. auriculiformis contained higher levels of these flavonoids (3.5- and 43-fold higher, respectively) than A. mangium. This suggests that higher levels of these compounds may contribute to heartrot resistance. Furthermore, both flavonoids had strong 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity and laccase inhibition. This suggests that the antifungal mechanism of these compounds may involve inhibition of fungal growth by quenching of free radicals produced by the extracellular fungal enzyme laccase" name="eprints.abstract" /> <meta content="2005-04" name="eprints.date" /> <meta content="published" name="eprints.date_type" /> <meta content="Journal of Chemical Ecology" name="eprints.publication" /> <meta content="31" name="eprints.volume" /> <meta content="4" name="eprints.number" /> <meta content="789-804" name="eprints.pagerange" /> <meta content="10.1007/s10886-005-3544-x" name="eprints.id_number" /> <meta content="TRUE" name="eprints.refereed" /> <meta content="http://dx.doi.org/10.1007/s10886-005-3544-x" name="eprints.official_url" /> <meta content="AAmi, D., Davidovi-Ami, D., Belo, D., Trinajsti, N. (2003) "Structure–radical scavenging activity relationships of flavonoids" Croat. Chem. Acta 76: 55-61 Backa, S., Gierer, J., Reitberger, T., Nilsson, T. (1993) "Hydroxyl radical activity associated with growth of white-rot fungi" Holzforschung 47: 181-187 Barry, K. M., Irianto, R. S. B., Santoso, E., Turjaman, M., Widyati, E., Sitepu, I., Mohammed, C. L. (2004) "Incidence of heartrot in harvest-age Acacia mangium in Indonesia, using a rapid survey method" For. Ecol. Manag. 190: 273-280 Barry, K. M., Mihara, R., Davies, N. W., Mitsunaga, T., and Mohammed, C. L. in press. Polyphenols in Acacia mangium and A. auriculiformis wood with reference to heartrot susceptibility. J. Wood Sci. Butler, L. G. (1992) "Antinutritional effects of condensed and hydrolysable tannins" In: Hemingway, R. W., Laks, P. E. eds. , Plant Polyphenols, Plenum Press, New York, pp 693-698 Chang, S. T., Wu, J. H., Wang, S. Y., Kang, P. L., Yang, N. S., Shyur, L. F. (2001) "Antioxidant activity of extracts from Acacia confusa bark and heartwood" J. Agric. Food Chem. 49: 3420-3424 Eyles, A., Davies, N. W., Mitsunaga, T., Mihara, R., Mohammed, C. (2004) "Role of Eucalyptus globules wound wood extractives: Evidence of superoxide dismutase-like activity" For. Pathol. 34: 225-232 Field, J. A., Lettinga, G. (1992) "Toxicity of tannic compounds to microorganisms" In: Hemingway, R. W., Laks, P. E. eds. , Plant Polyphenols, Plenum Press, New York, pp 673-692 Guillén, F., Gómez-Toribio, V., Martínez, M. J., Martínez, A. T. (2000) "Production of hydroxyl radical by the synergistic action of fungal laccase and aryl alcohol oxidase" Arch. Biochem. Biophys. 383: 142-147 Hagerman, A. E., Riedl, K. M., Jones, A., Sovik, K. N., Ritchard, N. T., Hartzfeld, P. W., Riechel, T. L. (1998) "High molecular weight plant polyphenols (tannins) as biological antioxidants" J. Agric. Food Chem. 46: 1887-1892 Harju, A. M., Kainulainen, P., Venäläinen, M., Tiitta, M., Viitanen, H. (2002) "Differences in resin acid concentration between brown-rot resistant and susceptible Scots pine heartwood" Holzforschung 56: 479-486 Hart, J. H., Hillis, W. E. (1974) "Inhibition of wood-rotting fungi by stilbenes and other polyphenols in Eucalyptus sideroxylon" Phytopathology 64: 939-948 Hashida, K., Ohara, S., and Nishida, A. 2001. Biseibutsuniyoru shukugougata tannin no henshitsukyodou (in Japanese), p. 365, in Abstracts of the 51st Annual Meeting of the Japan Wood Research Society, Tokyo. ISSN 0549-3994. Haslam, E. (1988) "Plant polyphenols (syn. vegetable tannins) and chemical defense—a reappraisal" J. Chem. Ecol. 14: 1789-1806 Hillis, W. E. (1987) Heartwood and Tree Exudates, Springer-Verlag, Berlin, p. 268 Ito, S. 2002. The infection of heart rot and disease severity on several Acacia species in SAFODA plantations. Study Report of SAFODA–JICA Project, Tsu, Mie, Japan. Ito, S. and Nanis, L. H. 1994. Heart rot on Acacia mangium in SAFODA plantation. Saba Reafforestation Technical Development and Training Project Study Report, Chin Chi Printing Works, Sabah, Malaysia. Ito, S., Nanis, L. H. (1997) "Survey of heart rot on Acacia mangium in Sabah, Malaysia" Jpn. Agric. Res. Q. 31: 65-71 Juntheikki, M. R., Julkunen-Titto, R. (2000) "Inhibition of -glucosidase and esterase by tannin from Betula, Salix, and Pinus species" J. Chem. Ecol. 26: 1151-1165 Kishino, M., Ohi, H., Yamaguchi, A. (1995) "Characteristics of methanol extractives from Chengal wood and their antifungal properties (in Japanese)" Mokuzai Gakkaishi 41: 444-447 Lange, W., Hashim, R. (2001) "The composition of he extractives from unaffected and heartrot affected heartwood of Acacia mangium Willd" Holz Roh-Werkst. 59: 61-66 Lee, S. S., Yahya, N. S. (1999) "Fungi associated with heart rot of Acacia mangium trees in peninsular Malaysia and East Kalimantan" J. Trop. For. Sci. 11: 240-254 Lee, S. S., Zakaria, M. (1993) "Fungi associated with heart rot of Acacia mangium in Peninsular Malaysia" J. Trop. For. Sci. 5: 479-484 Lee, S. S., Teng, S. Y., Lim, M. T., Kader, R. A. (1988) "Discolouration and heart rot of Acacia mangium Willd—some preliminary results" J. Trop. For. Sci. 1: 170-177 Leonowicz, A., Grzywnowicz, K. (1981) "Quantitative estimation of laccase forms in some white-rot fungi using syringaldazine as a substrate" Enzyme Microb. Technol. 3: 55-58 Mahmud, S., Lee, S. S., Ahmad, H. H. (1993) "A survey of heart rot in some plantation of Acacia mangium in Sabah" J. Trop. For. Sci. 6: 37-47 Mihara, R., Mitsunaga, T., Fukui, Y., Nakai, M., Yamaji, N., Shibata, H. (2004) "A novel acylated quercetin tetraglycoside from oolong tea (Camelia sinensis) extracts" Tetrahedron Lett. 45: 5077-5080 Mila, I., Scalbert, A., Expert, D. (1996) "Iron withholding by plant pathogens and resistance to pathogens and rots" Phytochemistry 42: 1551-1555 Mitsunaga, T., Abe, I., Kontani, M., Ono, H., Tanaka, T. (1997) "Inhibitory effects of bark proanthocyanidins on the activities of glucosyltransferases of Streptococcus soburinus" J. WoodChem. Technol. 17: 327-340 Mitsunaga, T., Nagamatsu, K., Nishii, T., Ohga, S. (1999) "Effect of bark polyphenols on mycelial growth of some basidiomycetes (in Japanese)" Trans. Mycol. Soc. Jpn. 40: 91-96 Quraishi, M. A., Ahmad, M. (1973) "Diseases of Acacia modesta wall" Pak. J. For. 23: 27-32 Sas Institute, Inc. 1999. SAS for Windows Version 8. SAS Institute, Inc., Cary, NC, USA. Scalbert, A. (1991) "Antimicrobial properties of tannins" Phytochemistry 30: 3875-3883 Scalbert, A. (1992) "Tannins in woods and their contribution to microbial decay prevention" In: Hemingway, R. W., Laks, P. E. eds. , Plant Polyphenols, Plenum Press, New York, pp 935-952 Shimizu, K., Kondo, R., Sakai, K., Lee, S. H., Sato, H. (1998) "The inhibitory components from Artocarpus incisus on melanin biosynthesis" Plant Med. 64: 408-412 Stalpers, J. A. (1978) "Identification of wood-inhabiting fungi in pure culture" Stud. Mycol. 16: 1-248 Tachi, M., Tange, J., Nagatomi, W., Suzuki, Y., Terashima, N., Yasuda, S. (1989) "Manufacture of wood-cement boards. IV. Cement-hardening inhibitory compounds of Malaysian fast-growing tree, Acacia mangium" Mokuzai Gakkaishi 35: 731-735 Tanaka, H., Itakura, S., Enoki, A. (1999) "Hydroxyl radical generation by an extracellular low-molecular-weight substance and phenol oxidase activity during wood degradation by the white-rot basidiomycete Trametes versicolor" J. Biotechnol. 75: 57-70 Willför, S. M., Ahotupa, M. O., Hemming, J. E., Reunanen, M. H. T., Eklund, P.C., Sjöholm, R. E., Eckerman, C. S. E., Pohjamo, S. P., Holmbom, B. R. (2003) "Antioxidant activity of knotwood extractives and phenolic compounds of selected tree species" J. Agric. Food Chem. 51: 7600-7606 Wood, D. A. (1979) "A method for estimating biomass of Agaricus bisporus in a solid substrate, composted wheat straw" Biotechnol. Lett. 1: 255-260 Yoshimoto, T., Samejima, M., Kimura, T., Yoshitomi, K., Nakanishi, K., Mori, K. (1984) "Growth inhibitors of Trichoderma sp. in hot-water extractives from barks of Quercus sp. (in Japanese)" Mokuzai Gakkaishi 30: 244-250 " name="eprints.referencetext" /> <meta content="Mihara, R. and Barry, K.M. and Mohammed, C.L. and Mitsunaga, T. (2005) Comparison of antifungal and antioxidant activities of Acacia mangium and A. auriculiformis heartwood extracts. Journal of Chemical Ecology, 31 (4). pp. 789-804." name="eprints.citation" /> <meta content="http://eprints.utas.edu.au/2091/1/Mihara_et_al_JOCE_2005.pdf" name="eprints.document_url" /> <link rel="schema.DC" href="http://purl.org/DC/elements/1.0/" /> <meta content="Comparison of antifungal and antioxidant activities of Acacia mangium and A. auriculiformis heartwood extracts" name="DC.title" /> <meta content="Mihara, R." name="DC.creator" /> <meta content="Barry, K.M." name="DC.creator" /> <meta content="Mohammed, C.L." name="DC.creator" /> <meta content="Mitsunaga, T." name="DC.creator" /> <meta content="270305 Mycology" name="DC.subject" /> <meta content="300603 Pests, Health and Diseases" name="DC.subject" /> <meta content="250302 Biological and Medical Chemistry" name="DC.subject" /> <meta content="The effect of heartwood extracts from Acacia mangium (heartrot-susceptible) and A. auriculiformis (heartrot-resistant) was examined on the growth of wood rotting fungi with in vitro assays. A. auriculiformis heartwood extracts had higher antifungal activity than A. mangium. The compounds 3,4,7,8-tetrahydroxyflavanone and teracacidin (the most abundant flavonoids in both species) showed antifungal activity. A. auriculiformis contained higher levels of these flavonoids (3.5- and 43-fold higher, respectively) than A. mangium. This suggests that higher levels of these compounds may contribute to heartrot resistance. Furthermore, both flavonoids had strong 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity and laccase inhibition. This suggests that the antifungal mechanism of these compounds may involve inhibition of fungal growth by quenching of free radicals produced by the extracellular fungal enzyme laccase" name="DC.description" /> <meta content="2005-04" name="DC.date" /> <meta content="Article" name="DC.type" /> <meta content="PeerReviewed" name="DC.type" /> <meta content="application/pdf" name="DC.format" /> <meta content="http://eprints.utas.edu.au/2091/1/Mihara_et_al_JOCE_2005.pdf" name="DC.identifier" /> <meta content="http://dx.doi.org/10.1007/s10886-005-3544-x" name="DC.relation" /> <meta content="Mihara, R. and Barry, K.M. and Mohammed, C.L. and Mitsunaga, T. (2005) Comparison of antifungal and antioxidant activities of Acacia mangium and A. auriculiformis heartwood extracts. 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border: solid 1px #ccc; padding: 3px"><tr> <td align="left"><a href="http://eprints.utas.edu.au/cgi/users/home">Login</a> | <a href="http://eprints.utas.edu.au/cgi/register">Create Account</a></td> <td align="right" style="white-space: nowrap"> <form method="get" accept-charset="utf-8" action="http://eprints.utas.edu.au/cgi/search" style="display:inline"> <input class="ep_tm_searchbarbox" size="20" type="text" name="q" /> <input class="ep_tm_searchbarbutton" value="Search" type="submit" name="_action_search" /> <input type="hidden" name="_order" value="bytitle" /> <input type="hidden" name="basic_srchtype" value="ALL" /> <input type="hidden" name="_satisfyall" value="ALL" /> </form> </td> </tr></table></td></tr> <tr> <td class="toplinks"><!-- InstanceBeginEditable name="content" --> <div align="center"> <table width="720" class="ep_tm_main"><tr><td align="left"> <h1 class="ep_tm_pagetitle">Comparison of antifungal and antioxidant activities of Acacia mangium and A. auriculiformis heartwood extracts</h1> <p style="margin-bottom: 1em" class="not_ep_block"><span class="person_name">Mihara, R.</span> and <span class="person_name">Barry, K.M.</span> and <span class="person_name">Mohammed, C.L.</span> and <span class="person_name">Mitsunaga, T.</span> (2005) <xhtml:em>Comparison of antifungal and antioxidant activities of Acacia mangium and A. auriculiformis heartwood extracts.</xhtml:em> Journal of Chemical Ecology, 31 (4). pp. 789-804.</p><p style="margin-bottom: 1em" class="not_ep_block"></p><table style="margin-bottom: 1em" class="not_ep_block"><tr><td valign="top" style="text-align:center"><a href="http://eprints.utas.edu.au/2091/1/Mihara_et_al_JOCE_2005.pdf"><img alt="[img]" src="http://eprints.utas.edu.au/style/images/fileicons/application_pdf.png" class="ep_doc_icon" border="0" /></a></td><td valign="top"><a href="http://eprints.utas.edu.au/2091/1/Mihara_et_al_JOCE_2005.pdf"><span class="ep_document_citation">PDF</span></a> - Full text restricted - Requires a PDF viewer<br />511Kb</td><td><form method="get" accept-charset="utf-8" action="http://eprints.utas.edu.au/cgi/request_doc"><input accept-charset="utf-8" value="2631" name="docid" type="hidden" /><div class=""><input value="Request a copy" name="_action_null" class="ep_form_action_button" onclick="return EPJS_button_pushed( '_action_null' )" type="submit" /> </div></form></td></tr></table><p style="margin-bottom: 1em" class="not_ep_block">Official URL: <a href="http://dx.doi.org/10.1007/s10886-005-3544-x">http://dx.doi.org/10.1007/s10886-005-3544-x</a></p><div class="not_ep_block"><h2>Abstract</h2><p style="padding-bottom: 16px; text-align: left; margin: 1em auto 0em auto">The effect of heartwood extracts from Acacia mangium (heartrot-susceptible) and A. auriculiformis (heartrot-resistant) was examined on the growth of wood rotting fungi with in vitro assays. A. auriculiformis heartwood extracts had higher antifungal activity than A. mangium. The compounds 3,4,7,8-tetrahydroxyflavanone and teracacidin (the most abundant flavonoids in both species) showed antifungal activity. A. auriculiformis contained higher levels of these flavonoids (3.5- and 43-fold higher, respectively) than A. mangium. This suggests that higher levels of these compounds may contribute to heartrot resistance. Furthermore, both flavonoids had strong 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity and laccase inhibition. This suggests that the antifungal mechanism of these compounds may involve inhibition of fungal growth by quenching of free radicals produced by the extracellular fungal enzyme laccase</p></div><table style="margin-bottom: 1em" cellpadding="3" class="not_ep_block" border="0"><tr><th valign="top" class="ep_row">Item Type:</th><td valign="top" class="ep_row">Article</td></tr><tr><th valign="top" class="ep_row">Additional Information:</th><td valign="top" class="ep_row">The original publication is available at www.springerlink.com</td></tr><tr><th valign="top" class="ep_row">Keywords:</th><td valign="top" class="ep_row">Acacia mangium - A. auriculiformis - heartrot - heartwood extracts - flavanone - Phellinus noxius - P. badius - antifungal activity - antioxidant - laccase inhibition</td></tr><tr><th valign="top" class="ep_row">Subjects:</th><td valign="top" class="ep_row"><a href="http://eprints.utas.edu.au/view/subjects/270305.html">270000 Biological Sciences > 270300 Microbiology > 270305 Mycology</a><br /><a href="http://eprints.utas.edu.au/view/subjects/300603.html">300000 Agricultural, Veterinary and Environmental Sciences > 300600 Forestry Sciences > 300603 Pests, Health and Diseases</a><br /><a href="http://eprints.utas.edu.au/view/subjects/250302.html">250000 Chemical Sciences > 250300 Organic Chemistry > 250302 Biological and Medical Chemistry</a></td></tr><tr><th valign="top" class="ep_row">ID Code:</th><td valign="top" class="ep_row">2091</td></tr><tr><th valign="top" class="ep_row">Deposited By:</th><td valign="top" class="ep_row"><span class="ep_name_citation"><span class="person_name">Dr Karen Barry</span></span></td></tr><tr><th valign="top" class="ep_row">Deposited On:</th><td valign="top" class="ep_row">11 Oct 2007 16:00</td></tr><tr><th valign="top" class="ep_row">Last Modified:</th><td valign="top" class="ep_row">09 Jan 2008 02:30</td></tr><tr><th valign="top" class="ep_row">ePrint Statistics:</th><td valign="top" class="ep_row"><a target="ePrintStats" href="/es/index.php?action=show_detail_eprint;id=2091;">View statistics for this ePrint</a></td></tr></table><p align="right">Repository Staff Only: <a href="http://eprints.utas.edu.au/cgi/users/home?screen=EPrint::View&eprintid=2091">item control page</a></p> </td></tr></table> </div> <!-- InstanceEndEditable --></td> </tr> <tr> <td><!-- #BeginLibraryItem "/Library/footer_eprints.lbi" --> <table width="795" border="0" align="left" cellpadding="0" class="footer"> <tr valign="top"> <td colspan="2"><div align="center"><a href="http://www.utas.edu.au">UTAS home</a> | <a href="http://www.utas.edu.au/library/">Library home</a> | <a href="/">ePrints home</a> | <a href="/contact.html">contact</a> | <a href="/information.html">about</a> | <a href="/view/">browse</a> | <a href="/perl/search/simple">search</a> | <a href="/perl/register">register</a> | <a href="/perl/users/home">user area</a> | <a href="/help/">help</a></div><br /></td> </tr> <tr><td colspan="2"><p><img src="/images/eprints/footerline.gif" width="100%" height="4" /></p></td></tr> <tr valign="top"> <td width="68%" class="footer">Authorised by the University Librarian<br /> © University of Tasmania ABN 30 764 374 782<br /> <a href="http://www.utas.edu.au/cricos/">CRICOS Provider Code 00586B</a> | <a href="http://www.utas.edu.au/copyright/copyright_disclaimers.html">Copyright & Disclaimers</a> | <a href="http://www.utas.edu.au/accessibility/index.html">Accessibility</a> | <a href="http://eprints.utas.edu.au/feedback/">Site Feedback</a> </td> <td width="32%"><div align="right"> <p align="right" class="NoPrint"><a href="http://www.utas.edu.au/"><img src="http://www.utas.edu.au/shared/logos/unioftasstrip.gif" alt="University of Tasmania Home Page" width="260" height="16" border="0" align="right" /></a></p> <p align="right" class="NoPrint"><a href="http://www.utas.edu.au/"><br /> </a></p> </div></td> </tr> <tr valign="top"> <td><p> </p></td> <td><div align="right"><span class="NoPrint"><a href="http://www.eprints.org/software/"><img src="/images/eprintslogo.gif" alt="ePrints logo" width="77" height="29" border="0" align="bottom" /></a></span></div></td> </tr> </table> <!-- #EndLibraryItem --> <div align="center"></div></td> </tr> </table> </body> </html>