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    <title>UTas ePrints - Effects of Incubation Temperature on Growth and Production of Exopolysaccharides by an Antarctic Sea Ice Bacterium Grown in Batch Culture</title>
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    <meta content="Nichols, C.A.M." name="eprints.creators_name" />
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<meta content="Effects of Incubation Temperature on Growth and Production of Exopolysaccharides by an Antarctic Sea Ice Bacterium Grown in Batch Culture" name="eprints.title" />
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<meta content="The sea ice microbial community plays a key role in the productivity of the Southern Ocean. Exopolysaccharide
(EPS) is a major component of the exopolymer secreted by many marine bacteria to enhance survival
and is abundant in sea ice brine channels, but little is known about its function there. This study investigated
the effects of temperature on EPS production in batch culture by CAM025, a marine bacterium isolated from
sea ice sampled from the Southern Ocean. Previous studies have shown that CAM025 is a member of the genus
Pseudoalteromonas and therefore belongs to a group found to be abundant in sea ice by culture-dependent and
-independent techniques. Batch cultures were grown at 2°C, 10°C, and 20°C, and cell number, optical density,
pH, glucose concentration, and viscosity were monitored. The yield of EPS at 2°C and 10°C was 30 times
higher than at 20°C, which is the optimum growth temperature for many psychrotolerant strains. EPS may
have a cryoprotective role in brine channels of sea ice, where extremes of high salinity and low temperature
impose pressures on microbial growth and survival. The EPS produced at 2°C and 10°C had a higher uronic
acid content than that produced at 20°C. The availability of iron as a trace metal is of critical importance in
the Southern Ocean, where it is known to limit primary production. EPS from strain CAM025 is polyanionic
and may bind dissolved cations such at trace metals, and therefore the presence of bacterial EPS in the
Antarctic marine environment may have important ecological implications." name="eprints.abstract" />
<meta content="2005" name="eprints.date" />
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<meta content="Applied and Environmental Microbiology" name="eprints.publication" />
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<meta content="1. Alldredge, A., and G. A. Jackson. 1995. Aggregation in marine systems.
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and Space Administration, Washington, D.C." name="eprints.referencetext" />
<meta content="Nichols, C.A.M. and Bowman, John P. and Guezennec, J. (2005) Effects of Incubation Temperature on Growth and Production of Exopolysaccharides by an Antarctic Sea Ice Bacterium Grown in Batch Culture. Applied and Environmental Microbiology, 71 (7). pp. 3519-3523. ISSN 0099-2240" name="eprints.citation" />
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and is abundant in sea ice brine channels, but little is known about its function there. This study investigated
the effects of temperature on EPS production in batch culture by CAM025, a marine bacterium isolated from
sea ice sampled from the Southern Ocean. Previous studies have shown that CAM025 is a member of the genus
Pseudoalteromonas and therefore belongs to a group found to be abundant in sea ice by culture-dependent and
-independent techniques. Batch cultures were grown at 2°C, 10°C, and 20°C, and cell number, optical density,
pH, glucose concentration, and viscosity were monitored. The yield of EPS at 2°C and 10°C was 30 times
higher than at 20°C, which is the optimum growth temperature for many psychrotolerant strains. EPS may
have a cryoprotective role in brine channels of sea ice, where extremes of high salinity and low temperature
impose pressures on microbial growth and survival. The EPS produced at 2°C and 10°C had a higher uronic
acid content than that produced at 20°C. The availability of iron as a trace metal is of critical importance in
the Southern Ocean, where it is known to limit primary production. EPS from strain CAM025 is polyanionic
and may bind dissolved cations such at trace metals, and therefore the presence of bacterial EPS in the
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    <h1 class="ep_tm_pagetitle">Effects of Incubation Temperature on Growth and Production of Exopolysaccharides by an Antarctic Sea Ice Bacterium Grown in Batch Culture</h1>
    <p style="margin-bottom: 1em" class="not_ep_block"><span class="person_name">Nichols, C.A.M.</span> and <span class="person_name">Bowman, John P.</span> and <span class="person_name">Guezennec, J.</span> (2005) <xhtml:em>Effects of Incubation Temperature on Growth and Production of Exopolysaccharides by an Antarctic Sea Ice Bacterium Grown in Batch Culture.</xhtml:em> Applied and Environmental Microbiology, 71 (7). pp. 3519-3523. ISSN 0099-2240</p><p style="margin-bottom: 1em" class="not_ep_block"></p><table style="margin-bottom: 1em" class="not_ep_block"><tr><td valign="top" style="text-align:center"><a onmouseover="EPJS_ShowPreview( event, 'doc_preview_2669' );" href="http://eprints.utas.edu.au/2108/1/Nichols_etal_2005.pdf" onmouseout="EPJS_HidePreview( event, 'doc_preview_2669' );"><img alt="[img]" src="http://eprints.utas.edu.au/style/images/fileicons/application_pdf.png" class="ep_doc_icon" border="0" /></a><div class="ep_preview" id="doc_preview_2669"><table><tr><td><img alt="" src="http://eprints.utas.edu.au/2108/thumbnails/1/preview.png" class="ep_preview_image" border="0" /><div class="ep_preview_title">Preview</div></td></tr></table></div></td><td valign="top"><a href="http://eprints.utas.edu.au/2108/1/Nichols_etal_2005.pdf"><span class="ep_document_citation">PDF</span></a> - Requires a PDF viewer<br />118Kb</td></tr></table><p style="margin-bottom: 1em" class="not_ep_block">Official URL: <a href="http://dx.doi.org/10.1128/AEM.71.7.3519–3523.2005">http://dx.doi.org/10.1128/AEM.71.7.3519–3523.2005</a></p><div class="not_ep_block"><h2>Abstract</h2><p style="padding-bottom: 16px; text-align: left; margin: 1em auto 0em auto">The sea ice microbial community plays a key role in the productivity of the Southern Ocean. Exopolysaccharide&#13;
(EPS) is a major component of the exopolymer secreted by many marine bacteria to enhance survival&#13;
and is abundant in sea ice brine channels, but little is known about its function there. This study investigated&#13;
the effects of temperature on EPS production in batch culture by CAM025, a marine bacterium isolated from&#13;
sea ice sampled from the Southern Ocean. Previous studies have shown that CAM025 is a member of the genus&#13;
Pseudoalteromonas and therefore belongs to a group found to be abundant in sea ice by culture-dependent and&#13;
-independent techniques. Batch cultures were grown at 2°C, 10°C, and 20°C, and cell number, optical density,&#13;
pH, glucose concentration, and viscosity were monitored. The yield of EPS at 2°C and 10°C was 30 times&#13;
higher than at 20°C, which is the optimum growth temperature for many psychrotolerant strains. EPS may&#13;
have a cryoprotective role in brine channels of sea ice, where extremes of high salinity and low temperature&#13;
impose pressures on microbial growth and survival. The EPS produced at 2°C and 10°C had a higher uronic&#13;
acid content than that produced at 20°C. The availability of iron as a trace metal is of critical importance in&#13;
the Southern Ocean, where it is known to limit primary production. EPS from strain CAM025 is polyanionic&#13;
and may bind dissolved cations such at trace metals, and therefore the presence of bacterial EPS in the&#13;
Antarctic marine environment may have important ecological implications.</p></div><table style="margin-bottom: 1em" cellpadding="3" class="not_ep_block" border="0"><tr><th valign="top" class="ep_row">Item Type:</th><td valign="top" class="ep_row">Article</td></tr><tr><th valign="top" class="ep_row">Additional Information:</th><td valign="top" class="ep_row"> </td></tr><tr><th valign="top" class="ep_row">Subjects:</th><td valign="top" class="ep_row"><a href="http://eprints.utas.edu.au/view/subjects/270307.html">270000 Biological Sciences &gt; 270300 Microbiology &gt; 270307 Microbial Ecology</a></td></tr><tr><th valign="top" class="ep_row">ID Code:</th><td valign="top" class="ep_row">2108</td></tr><tr><th valign="top" class="ep_row">Deposited By:</th><td valign="top" class="ep_row"><span class="ep_name_citation"><span class="person_name">Assoc. Prof. John P. Bowman</span></span></td></tr><tr><th valign="top" class="ep_row">Deposited On:</th><td valign="top" class="ep_row">09 Oct 2007 15:20</td></tr><tr><th valign="top" class="ep_row">Last Modified:</th><td valign="top" class="ep_row">09 Jan 2008 02:30</td></tr><tr><th valign="top" class="ep_row">ePrint Statistics:</th><td valign="top" class="ep_row"><a target="ePrintStats" href="/es/index.php?action=show_detail_eprint;id=2108;">View statistics for this ePrint</a></td></tr></table><p align="right">Repository Staff Only: <a href="http://eprints.utas.edu.au/cgi/users/home?screen=EPrint::View&amp;eprintid=2108">item control page</a></p>
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