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  5.     <title>UTas ePrints - DNA as a dietary biomarker in Antarctic krill, Euphausia superba</title>
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  13.     <meta content="Passmore, A.J." name="eprints.creators_name" />
  14. <meta content="Jarman, Simon N." name="eprints.creators_name" />
  15. <meta content="Swadling, Kerrie M." name="eprints.creators_name" />
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  23. <meta content="DNA as a dietary biomarker in Antarctic krill, Euphausia superba" name="eprints.title" />
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  27. <meta content="diet analysis — foodweb — trophic" name="eprints.keywords" />
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  29. <meta content="The diet of Antarctic krill (Euphausia superba) has
  30. been studied using a variety of techniques, but
  31. current methods still suffer from problems that are
  32. difficult to solve. This study examined an alternative
  33. approach utilizing DNA as a prey biomarker.
  34. Methods were developed for the preservation, extraction,
  35. and identification of prey DNA from krill
  36. collected in the field. Group-specific polymerase
  37. chain reaction (PCR) was used to amplify diatom
  38. prey (Phylum: Bacillariophyta) and the results from
  39. DNA clone libraries were compared with microscopic
  40. diet analysis. DNA analysis was superior to
  41. microscopy for prey detection. However, differences
  42. in prey relative abundance estimates between the
  43. two techniques suggested some bias in the DNAbased
  44. estimates. Quantification showed that large
  45. amounts of prey DNA had been successfully preserved
  46. and extracted. Overall the results suggest
  47. that the application of DNA-based diet analysis to
  48. krill warrants further investigation, particularly for
  49. prey that are difficult to study using other methods." name="eprints.abstract" />
  50. <meta content="2006-12" name="eprints.date" />
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  58. <meta content="http://dx.doi.org/10.1007/s10126-005-6088-8" name="eprints.official_url" />
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  235. 203–214" name="eprints.referencetext" />
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  244. <meta content="The diet of Antarctic krill (Euphausia superba) has
  245. been studied using a variety of techniques, but
  246. current methods still suffer from problems that are
  247. difficult to solve. This study examined an alternative
  248. approach utilizing DNA as a prey biomarker.
  249. Methods were developed for the preservation, extraction,
  250. and identification of prey DNA from krill
  251. collected in the field. Group-specific polymerase
  252. chain reaction (PCR) was used to amplify diatom
  253. prey (Phylum: Bacillariophyta) and the results from
  254. DNA clone libraries were compared with microscopic
  255. diet analysis. DNA analysis was superior to
  256. microscopy for prey detection. However, differences
  257. in prey relative abundance estimates between the
  258. two techniques suggested some bias in the DNAbased
  259. estimates. Quantification showed that large
  260. amounts of prey DNA had been successfully preserved
  261. and extracted. Overall the results suggest
  262. that the application of DNA-based diet analysis to
  263. krill warrants further investigation, particularly for
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  378.     <h1 class="ep_tm_pagetitle">DNA as a dietary biomarker in Antarctic krill, Euphausia superba</h1>
  379.     <p style="margin-bottom: 1em" class="not_ep_block"><span class="person_name">Passmore, A.J.</span> and <span class="person_name">Jarman, Simon N.</span> and <span class="person_name">Swadling, Kerrie M.</span> (2006) <xhtml:em>DNA as a dietary biomarker in Antarctic krill, Euphausia superba.</xhtml:em> Marine Biotechnology, 8 (6). pp. 686-696.</p><p style="margin-bottom: 1em" class="not_ep_block"></p><table style="margin-bottom: 1em" class="not_ep_block"><tr><td valign="top" style="text-align:center"><a href="http://eprints.utas.edu.au/2162/1/Passmoreetal2006_DNA.pdf"><img alt="[img]" src="http://eprints.utas.edu.au/style/images/fileicons/application_pdf.png" class="ep_doc_icon" border="0" /></a></td><td valign="top"><a href="http://eprints.utas.edu.au/2162/1/Passmoreetal2006_DNA.pdf"><span class="ep_document_citation">PDF</span></a> - Full text restricted - Requires a PDF viewer<br />336Kb</td><td><form method="get" accept-charset="utf-8" action="http://eprints.utas.edu.au/cgi/request_doc"><input accept-charset="utf-8" value="2703" name="docid" type="hidden" /><div class=""><input value="Request a copy" name="_action_null" class="ep_form_action_button" onclick="return EPJS_button_pushed( '_action_null' )" type="submit" /> </div></form></td></tr></table><p style="margin-bottom: 1em" class="not_ep_block">Official URL: <a href="http://dx.doi.org/10.1007/s10126-005-6088-8">http://dx.doi.org/10.1007/s10126-005-6088-8</a></p><div class="not_ep_block"><h2>Abstract</h2><p style="padding-bottom: 16px; text-align: left; margin: 1em auto 0em auto">The diet of Antarctic krill (Euphausia superba) has&#13;
  380. been studied using a variety of techniques, but&#13;
  381. current methods still suffer from problems that are&#13;
  382. difficult to solve. This study examined an alternative&#13;
  383. approach utilizing DNA as a prey biomarker.&#13;
  384. Methods were developed for the preservation, extraction,&#13;
  385. and identification of prey DNA from krill&#13;
  386. collected in the field. Group-specific polymerase&#13;
  387. chain reaction (PCR) was used to amplify diatom&#13;
  388. prey (Phylum: Bacillariophyta) and the results from&#13;
  389. DNA clone libraries were compared with microscopic&#13;
  390. diet analysis. DNA analysis was superior to&#13;
  391. microscopy for prey detection. However, differences&#13;
  392. in prey relative abundance estimates between the&#13;
  393. two techniques suggested some bias in the DNAbased&#13;
  394. estimates. Quantification showed that large&#13;
  395. amounts of prey DNA had been successfully preserved&#13;
  396. and extracted. Overall the results suggest&#13;
  397. that the application of DNA-based diet analysis to&#13;
  398. krill warrants further investigation, particularly for&#13;
  399. prey that are difficult to study using other methods.</p></div><table style="margin-bottom: 1em" cellpadding="3" class="not_ep_block" border="0"><tr><th valign="top" class="ep_row">Item Type:</th><td valign="top" class="ep_row">Article</td></tr><tr><th valign="top" class="ep_row">Additional Information:</th><td valign="top" class="ep_row">The original publication is available at www.springerlink.com</td></tr><tr><th valign="top" class="ep_row">Keywords:</th><td valign="top" class="ep_row">diet analysis — foodweb — trophic</td></tr><tr><th valign="top" class="ep_row">Subjects:</th><td valign="top" class="ep_row"><a href="http://eprints.utas.edu.au/view/subjects/270702.html">270000 Biological Sciences &gt; 270700 Ecology and Evolution &gt; 270702 Marine and Estuarine Ecology (incl. Marine Ichthyology)</a></td></tr><tr><th valign="top" class="ep_row">ID Code:</th><td valign="top" class="ep_row">2162</td></tr><tr><th valign="top" class="ep_row">Deposited By:</th><td valign="top" class="ep_row"><span class="ep_name_citation"><span class="person_name">Dr Kerrie Swadling</span></span></td></tr><tr><th valign="top" class="ep_row">Deposited On:</th><td valign="top" class="ep_row">18 Oct 2007 13:52</td></tr><tr><th valign="top" class="ep_row">Last Modified:</th><td valign="top" class="ep_row">09 Jan 2008 02:30</td></tr><tr><th valign="top" class="ep_row">ePrint Statistics:</th><td valign="top" class="ep_row"><a target="ePrintStats" href="/es/index.php?action=show_detail_eprint;id=2162;">View statistics for this ePrint</a></td></tr></table><p align="right">Repository Staff Only: <a href="http://eprints.utas.edu.au/cgi/users/home?screen=EPrint::View&amp;eprintid=2162">item control page</a></p>
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