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<title>UTas ePrints - Metallothionein-IIA promotes initial neurite elongation and postinjury reactive neurite growth and facilitates healing after focal cortical brain injury</title>
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<meta content="Chung, R.S." name="eprints.creators_name" />
<meta content="Vickers, J.C." name="eprints.creators_name" />
<meta content="Chuah, Meng Inn" name="eprints.creators_name" />
<meta content="West, A.K." name="eprints.creators_name" />
<meta content="rschung@utas.edu.au" name="eprints.creators_id" />
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<meta content="Inn.Chuah@utas.edu.au" name="eprints.creators_id" />
<meta content="awest@utas.edu.au" name="eprints.creators_id" />
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<meta content="2007-10-16 01:51:05" name="eprints.datestamp" />
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<meta content="Metallothionein-IIA promotes initial neurite elongation and postinjury reactive neurite growth and facilitates healing after focal cortical brain injury" name="eprints.title" />
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<meta content="metallothionein; reactive sprouting; neurite sprouting; neurite growth; cortical brain injury; wound healing" name="eprints.keywords" />
<meta content="Metallothioneins (MTs) are small, cysteine-rich, metal binding proteins. Their function has often been considered as stress-related
proteins capable of protecting cells from heavy metal toxicity and oxidative free radicals. However, recent interest has focused on the
brain-specific MT-III isoform, which has neurite-inhibitory properties. To investigate the effect of another MT isoform, human MT-IIA,
on neurite growth, we used rat cortical neuron cultures. MT-IIA promoted a significant increase in the rate of initial neurite elongation of
individually plated neurons. We also investigated the effect of MT-IIA on the neuronal response to axonal transection in vitro. MT-IIA
promoted reactive axonal growth after injury, and, by 18 hr after transection, MT-IIA had promoted axonal growth across the injury tract.
Exogenous application of MT-IIA after cortical brain injury promoted wound healing, as observed by a significant decrease in cellular
degradation at 4 d after injury. Furthermore, MT-IIA-treated rats exhibited numerous SMI-312-immunoreactive axonal processes within
the injury tract. This was in contrast to vehicle-treated animals, in which few axonal sprouts were observed. By 7 d after injury, MT-IIA
treatment resulted in a total closing over of the injury tract by microglia, astrocytes, and reactive axonal processes. However, although
some reactive axonal processes were observed within the injury tract of vehicle-treated rats, the tract itself was almost never entirely
enclosed. These results are discussed in relation to a possible physiological role of metallothioneins in the brain, as well as in a therapeutic" name="eprints.abstract" />
<meta content="2003" name="eprints.date" />
<meta content="published" name="eprints.date_type" />
<meta content="Journal of Neuroscience" name="eprints.publication" />
<meta content="23" name="eprints.volume" />
<meta content="3336-3342" name="eprints.pagerange" />
<meta content="TRUE" name="eprints.refereed" />
<meta content="0270-6474" name="eprints.issn" />
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Vasak M, Hasler DW (2000) Metallothioneins: new structural and functional
insights. Curr Opin Chem Biol 4:177–183" name="eprints.referencetext" />
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<meta content="West, A.K." name="DC.creator" />
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<meta content="Metallothioneins (MTs) are small, cysteine-rich, metal binding proteins. Their function has often been considered as stress-related
proteins capable of protecting cells from heavy metal toxicity and oxidative free radicals. However, recent interest has focused on the
brain-specific MT-III isoform, which has neurite-inhibitory properties. To investigate the effect of another MT isoform, human MT-IIA,
on neurite growth, we used rat cortical neuron cultures. MT-IIA promoted a significant increase in the rate of initial neurite elongation of
individually plated neurons. We also investigated the effect of MT-IIA on the neuronal response to axonal transection in vitro. MT-IIA
promoted reactive axonal growth after injury, and, by 18 hr after transection, MT-IIA had promoted axonal growth across the injury tract.
Exogenous application of MT-IIA after cortical brain injury promoted wound healing, as observed by a significant decrease in cellular
degradation at 4 d after injury. Furthermore, MT-IIA-treated rats exhibited numerous SMI-312-immunoreactive axonal processes within
the injury tract. This was in contrast to vehicle-treated animals, in which few axonal sprouts were observed. By 7 d after injury, MT-IIA
treatment resulted in a total closing over of the injury tract by microglia, astrocytes, and reactive axonal processes. However, although
some reactive axonal processes were observed within the injury tract of vehicle-treated rats, the tract itself was almost never entirely
enclosed. These results are discussed in relation to a possible physiological role of metallothioneins in the brain, as well as in a therapeutic" name="DC.description" />
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<h1 class="ep_tm_pagetitle">Metallothionein-IIA promotes initial neurite elongation and postinjury reactive neurite growth and facilitates healing after focal cortical brain injury</h1>
<p style="margin-bottom: 1em" class="not_ep_block"><span class="person_name">Chung, R.S.</span> and <span class="person_name">Vickers, J.C.</span> and <span class="person_name">Chuah, Meng Inn</span> and <span class="person_name">West, A.K.</span> (2003) <xhtml:em>Metallothionein-IIA promotes initial neurite elongation and postinjury reactive neurite growth and facilitates healing after focal cortical brain injury.</xhtml:em> Journal of Neuroscience, 23 . pp. 3336-3342. ISSN 0270-6474</p><p style="margin-bottom: 1em" class="not_ep_block"></p><table style="margin-bottom: 1em" class="not_ep_block"><tr><td valign="top" style="text-align:center"><a href="http://eprints.utas.edu.au/2229/1/Chung_etal._J.Neurosci.pdf"><img alt="[img]" src="http://eprints.utas.edu.au/style/images/fileicons/application_pdf.png" class="ep_doc_icon" border="0" /></a></td><td valign="top"><a href="http://eprints.utas.edu.au/2229/1/Chung_etal._J.Neurosci.pdf"><span class="ep_document_citation">PDF</span></a> - Full text restricted - Requires a PDF viewer<br />580Kb</td><td><form method="get" accept-charset="utf-8" action="http://eprints.utas.edu.au/cgi/request_doc"><input accept-charset="utf-8" value="2799" name="docid" type="hidden" /><div class=""><input value="Request a copy" name="_action_null" class="ep_form_action_button" onclick="return EPJS_button_pushed( '_action_null' )" type="submit" /> </div></form></td></tr></table><div class="not_ep_block"><h2>Abstract</h2><p style="padding-bottom: 16px; text-align: left; margin: 1em auto 0em auto">Metallothioneins (MTs) are small, cysteine-rich, metal binding proteins. Their function has often been considered as stress-related
proteins capable of protecting cells from heavy metal toxicity and oxidative free radicals. However, recent interest has focused on the
brain-specific MT-III isoform, which has neurite-inhibitory properties. To investigate the effect of another MT isoform, human MT-IIA,
on neurite growth, we used rat cortical neuron cultures. MT-IIA promoted a significant increase in the rate of initial neurite elongation of
individually plated neurons. We also investigated the effect of MT-IIA on the neuronal response to axonal transection in vitro. MT-IIA
promoted reactive axonal growth after injury, and, by 18 hr after transection, MT-IIA had promoted axonal growth across the injury tract.
Exogenous application of MT-IIA after cortical brain injury promoted wound healing, as observed by a significant decrease in cellular
degradation at 4 d after injury. Furthermore, MT-IIA-treated rats exhibited numerous SMI-312-immunoreactive axonal processes within
the injury tract. This was in contrast to vehicle-treated animals, in which few axonal sprouts were observed. By 7 d after injury, MT-IIA
treatment resulted in a total closing over of the injury tract by microglia, astrocytes, and reactive axonal processes. However, although
some reactive axonal processes were observed within the injury tract of vehicle-treated rats, the tract itself was almost never entirely
enclosed. These results are discussed in relation to a possible physiological role of metallothioneins in the brain, as well as in a therapeutic</p></div><table style="margin-bottom: 1em" cellpadding="3" class="not_ep_block" border="0"><tr><th valign="top" class="ep_row">Item Type:</th><td valign="top" class="ep_row">Article</td></tr><tr><th valign="top" class="ep_row">Keywords:</th><td valign="top" class="ep_row">metallothionein; reactive sprouting; neurite sprouting; neurite growth; cortical brain injury; wound healing</td></tr><tr><th valign="top" class="ep_row">Subjects:</th><td valign="top" class="ep_row"><a href="http://eprints.utas.edu.au/view/subjects/320000.html">320000 Medical and Health Sciences</a><br /><a href="http://eprints.utas.edu.au/view/subjects/270000.html">270000 Biological Sciences</a></td></tr><tr><th valign="top" class="ep_row">ID Code:</th><td valign="top" class="ep_row">2229</td></tr><tr><th valign="top" class="ep_row">Deposited By:</th><td valign="top" class="ep_row"><span class="ep_name_citation"><span class="person_name">A/Prof MI Chuah</span></span></td></tr><tr><th valign="top" class="ep_row">Deposited On:</th><td valign="top" class="ep_row">16 Oct 2007 12:51</td></tr><tr><th valign="top" class="ep_row">Last Modified:</th><td valign="top" class="ep_row">09 Jan 2008 02:30</td></tr><tr><th valign="top" class="ep_row">ePrint Statistics:</th><td valign="top" class="ep_row"><a target="ePrintStats" href="/es/index.php?action=show_detail_eprint;id=2229;">View statistics for this ePrint</a></td></tr></table><p align="right">Repository Staff Only: <a href="http://eprints.utas.edu.au/cgi/users/home?screen=EPrint::View&eprintid=2229">item control page</a></p>
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