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  5.     <title>UTas ePrints - Metallothionein-IIA promotes initial neurite elongation and postinjury reactive neurite growth and facilitates healing after focal cortical brain injury</title>
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  13.     <meta content="Chung, R.S." name="eprints.creators_name" />
  14. <meta content="Vickers, J.C." name="eprints.creators_name" />
  15. <meta content="Chuah, Meng Inn" name="eprints.creators_name" />
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  25. <meta content="Metallothionein-IIA promotes initial neurite elongation and postinjury reactive neurite growth and facilitates healing after focal cortical brain injury" name="eprints.title" />
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  30. <meta content="metallothionein; reactive sprouting; neurite sprouting; neurite growth; cortical brain injury; wound healing" name="eprints.keywords" />
  31. <meta content="Metallothioneins (MTs) are small, cysteine-rich, metal binding proteins. Their function has often been considered as stress-related
  32. proteins capable of protecting cells from heavy metal toxicity and oxidative free radicals. However, recent interest has focused on the
  33. brain-specific MT-III isoform, which has neurite-inhibitory properties. To investigate the effect of another MT isoform, human MT-IIA,
  34. on neurite growth, we used rat cortical neuron cultures. MT-IIA promoted a significant increase in the rate of initial neurite elongation of
  35. individually plated neurons. We also investigated the effect of MT-IIA on the neuronal response to axonal transection in vitro. MT-IIA
  36. promoted reactive axonal growth after injury, and, by 18 hr after transection, MT-IIA had promoted axonal growth across the injury tract.
  37. Exogenous application of MT-IIA after cortical brain injury promoted wound healing, as observed by a significant decrease in cellular
  38. degradation at 4 d after injury. Furthermore, MT-IIA-treated rats exhibited numerous SMI-312-immunoreactive axonal processes within
  39. the injury tract. This was in contrast to vehicle-treated animals, in which few axonal sprouts were observed. By 7 d after injury, MT-IIA
  40. treatment resulted in a total closing over of the injury tract by microglia, astrocytes, and reactive axonal processes. However, although
  41. some reactive axonal processes were observed within the injury tract of vehicle-treated rats, the tract itself was almost never entirely
  42. enclosed. These results are discussed in relation to a possible physiological role of metallothioneins in the brain, as well as in a therapeutic" name="eprints.abstract" />
  43. <meta content="2003" name="eprints.date" />
  44. <meta content="published" name="eprints.date_type" />
  45. <meta content="Journal of Neuroscience" name="eprints.publication" />
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  50. <meta content="Abdel-Mageed AB, Agrawal KC (1998) Activation of nuclear factor kappaB:
  51. potential role in metallothionein-mediated mitogenic response. Cancer
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  53. Adlard PA, West AK, Vickers JC (1998) Increased density of metallothionein
  54. I/II-immunopositive cortical glial cells in the early stages of Alzheimer’s
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  70. (2001) Gene expression profiling of acute spinal cord injury reveals
  71. spreading inflammatory signals and neuron loss. Physiol Genomics
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  74. AK (2002a) Sheep have an unusual variant of the brain specific metallothionein,
  75. MT-III. Biochem J 365:323–328.
  76. Chung RS, Vickers JC, Chuah MI, Eckhardt BL, West AK (2002b)
  77. Metallothionein-III inhibits initial neurite formation in developing neurons
  78. as well as post-injury, regenerative neurite sprouting. Exp Neurol
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  163. than metal binding properties. Biochemistry 34:4740–4747.
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  166. from preadipocytes in primary culture. Horm Metab Res 32:542–547.
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  174. amino acid metallothionein-like protein. Neuron 7:337–347.
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  176. prevents neurite extension and death of cortical neurons caused by high
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  180. 312 as tools to investigate the maturation of nerve cells and axonal patterns
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  182. Vasak M, Hasler DW (2000) Metallothioneins: new structural and functional
  183. insights. Curr Opin Chem Biol 4:177–183" name="eprints.referencetext" />
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  195. proteins capable of protecting cells from heavy metal toxicity and oxidative free radicals. However, recent interest has focused on the
  196. brain-specific MT-III isoform, which has neurite-inhibitory properties. To investigate the effect of another MT isoform, human MT-IIA,
  197. on neurite growth, we used rat cortical neuron cultures. MT-IIA promoted a significant increase in the rate of initial neurite elongation of
  198. individually plated neurons. We also investigated the effect of MT-IIA on the neuronal response to axonal transection in vitro. MT-IIA
  199. promoted reactive axonal growth after injury, and, by 18 hr after transection, MT-IIA had promoted axonal growth across the injury tract.
  200. Exogenous application of MT-IIA after cortical brain injury promoted wound healing, as observed by a significant decrease in cellular
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  318.     <h1 class="ep_tm_pagetitle">Metallothionein-IIA promotes initial neurite elongation and postinjury reactive neurite growth and facilitates healing after focal cortical brain injury</h1>
  319.     <p style="margin-bottom: 1em" class="not_ep_block"><span class="person_name">Chung, R.S.</span> and <span class="person_name">Vickers, J.C.</span> and <span class="person_name">Chuah, Meng Inn</span> and <span class="person_name">West, A.K.</span> (2003) <xhtml:em>Metallothionein-IIA promotes initial neurite elongation and postinjury reactive neurite growth and facilitates healing after focal cortical brain injury.</xhtml:em> Journal of Neuroscience, 23 . pp. 3336-3342. ISSN 0270-6474</p><p style="margin-bottom: 1em" class="not_ep_block"></p><table style="margin-bottom: 1em" class="not_ep_block"><tr><td valign="top" style="text-align:center"><a href="http://eprints.utas.edu.au/2229/1/Chung_etal._J.Neurosci.pdf"><img alt="[img]" src="http://eprints.utas.edu.au/style/images/fileicons/application_pdf.png" class="ep_doc_icon" border="0" /></a></td><td valign="top"><a href="http://eprints.utas.edu.au/2229/1/Chung_etal._J.Neurosci.pdf"><span class="ep_document_citation">PDF</span></a> - Full text restricted - Requires a PDF viewer<br />580Kb</td><td><form method="get" accept-charset="utf-8" action="http://eprints.utas.edu.au/cgi/request_doc"><input accept-charset="utf-8" value="2799" name="docid" type="hidden" /><div class=""><input value="Request a copy" name="_action_null" class="ep_form_action_button" onclick="return EPJS_button_pushed( '_action_null' )" type="submit" /> </div></form></td></tr></table><div class="not_ep_block"><h2>Abstract</h2><p style="padding-bottom: 16px; text-align: left; margin: 1em auto 0em auto">Metallothioneins (MTs) are small, cysteine-rich, metal binding proteins. Their function has often been considered as stress-related&#13;
  320. proteins capable of protecting cells from heavy metal toxicity and oxidative free radicals. However, recent interest has focused on the&#13;
  321. brain-specific MT-III isoform, which has neurite-inhibitory properties. To investigate the effect of another MT isoform, human MT-IIA,&#13;
  322. on neurite growth, we used rat cortical neuron cultures. MT-IIA promoted a significant increase in the rate of initial neurite elongation of&#13;
  323. individually plated neurons. We also investigated the effect of MT-IIA on the neuronal response to axonal transection in vitro. MT-IIA&#13;
  324. promoted reactive axonal growth after injury, and, by 18 hr after transection, MT-IIA had promoted axonal growth across the injury tract.&#13;
  325. Exogenous application of MT-IIA after cortical brain injury promoted wound healing, as observed by a significant decrease in cellular&#13;
  326. degradation at 4 d after injury. Furthermore, MT-IIA-treated rats exhibited numerous SMI-312-immunoreactive axonal processes within&#13;
  327. the injury tract. This was in contrast to vehicle-treated animals, in which few axonal sprouts were observed. By 7 d after injury, MT-IIA&#13;
  328. treatment resulted in a total closing over of the injury tract by microglia, astrocytes, and reactive axonal processes. However, although&#13;
  329. some reactive axonal processes were observed within the injury tract of vehicle-treated rats, the tract itself was almost never entirely&#13;
  330. enclosed. These results are discussed in relation to a possible physiological role of metallothioneins in the brain, as well as in a therapeutic</p></div><table style="margin-bottom: 1em" cellpadding="3" class="not_ep_block" border="0"><tr><th valign="top" class="ep_row">Item Type:</th><td valign="top" class="ep_row">Article</td></tr><tr><th valign="top" class="ep_row">Keywords:</th><td valign="top" class="ep_row">metallothionein; reactive sprouting; neurite sprouting; neurite growth; cortical brain injury; wound healing</td></tr><tr><th valign="top" class="ep_row">Subjects:</th><td valign="top" class="ep_row"><a href="http://eprints.utas.edu.au/view/subjects/320000.html">320000 Medical and Health Sciences</a><br /><a href="http://eprints.utas.edu.au/view/subjects/270000.html">270000 Biological Sciences</a></td></tr><tr><th valign="top" class="ep_row">ID Code:</th><td valign="top" class="ep_row">2229</td></tr><tr><th valign="top" class="ep_row">Deposited By:</th><td valign="top" class="ep_row"><span class="ep_name_citation"><span class="person_name">A/Prof MI Chuah</span></span></td></tr><tr><th valign="top" class="ep_row">Deposited On:</th><td valign="top" class="ep_row">16 Oct 2007 12:51</td></tr><tr><th valign="top" class="ep_row">Last Modified:</th><td valign="top" class="ep_row">09 Jan 2008 02:30</td></tr><tr><th valign="top" class="ep_row">ePrint Statistics:</th><td valign="top" class="ep_row"><a target="ePrintStats" href="/es/index.php?action=show_detail_eprint;id=2229;">View statistics for this ePrint</a></td></tr></table><p align="right">Repository Staff Only: <a href="http://eprints.utas.edu.au/cgi/users/home?screen=EPrint::View&amp;eprintid=2229">item control page</a></p>
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