<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN"
"http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd">
<html>
<head>
<title>UTas ePrints - The Pea Gene NA Encodes ent-Kaurenoic Acid Oxidase 1</title>
<script type="text/javascript" src="http://eprints.utas.edu.au/javascript/auto.js"><!-- padder --></script>
<style type="text/css" media="screen">@import url(http://eprints.utas.edu.au/style/auto.css);</style>
<style type="text/css" media="print">@import url(http://eprints.utas.edu.au/style/print.css);</style>
<link rel="icon" href="/images/eprints/favicon.ico" type="image/x-icon" />
<link rel="shortcut icon" href="/images/eprints/favicon.ico" type="image/x-icon" />
<link rel="Top" href="http://eprints.utas.edu.au/" />
<link rel="Search" href="http://eprints.utas.edu.au/cgi/search" />
<meta content="Davidson, Sandra E." name="eprints.creators_name" />
<meta content="Elliott, Robert C." name="eprints.creators_name" />
<meta content="Helliwell, Chris A." name="eprints.creators_name" />
<meta content="Poole, Andrew T." name="eprints.creators_name" />
<meta content="Reid, James B." name="eprints.creators_name" />
<meta content="" name="eprints.creators_id" />
<meta content="" name="eprints.creators_id" />
<meta content="" name="eprints.creators_id" />
<meta content="" name="eprints.creators_id" />
<meta content="James.Reid@utas.edu.au" name="eprints.creators_id" />
<meta content="article" name="eprints.type" />
<meta content="2007-10-30 03:59:11" name="eprints.datestamp" />
<meta content="2008-01-08 15:30:00" name="eprints.lastmod" />
<meta content="show" name="eprints.metadata_visibility" />
<meta content="The Pea Gene NA Encodes ent-Kaurenoic Acid Oxidase 1" name="eprints.title" />
<meta content="pub" name="eprints.ispublished" />
<meta content="320000" name="eprints.subjects" />
<meta content="restricted" name="eprints.full_text_status" />
<meta content="The gibberellin (GA)-deficient dwarf na mutant in pea tPisum sativum) has severely reduced internode elongation, reduced root growth, and decreased leaflet size. However, the seeds develop normally. Two genes, PsKAOl and PsKA02, encoding cytochrome P450 monooxygenases of the subfamily CYP88A were isolated. Both PsKAOl and PsKA02 had cllt-kaurenoic acid oxidase (KAO) activity, catalyzing the three steps of the GA biosynthetic 'pathway from ellt-kaurenoic acid to GA12 when expressed in yeast (Saccharolllyces cereuisiaes. In addition to the intermediates cnt-7n-hydroxykaurenoic acid and GAn-aldehyde, some additional products of the pea KAO activity wen! detected, induding ent-60',70'-dihydroxykaurenoic acid and 7(:l-hydroxykaurenolide. The NA gene encodes PsKA01, because in two independent mutant alleles, na-t and na-L, PsKAOl had altered sequences and the five-base deletion in PsKAOl associated with the tl17-1 allele cosegregated with the dwarf 1117 phenotype. P5KAOI was expressed in the stem, apical bud, leaf, pod, and root, organs in which GA levels have previously been shown to be reduced in IIIl plants. PsKA02 was expressed only in seeds and this may explain the normal seed development and normal GA biosynthesis in seeds of lla plants." name="eprints.abstract" />
<meta content="2003" name="eprints.date" />
<meta content="published" name="eprints.date_type" />
<meta content="Plant Physiology" name="eprints.publication" />
<meta content="131" name="eprints.volume" />
<meta content="1" name="eprints.number" />
<meta content="335-344" name="eprints.pagerange" />
<meta content="TRUE" name="eprints.refereed" />
<meta content="0032-0889" name="eprints.issn" />
<meta content="http://proquest.umi.com/pqdweb?did=288001151&sid=1&Fmt=4&clientId=20931&RQT=309&VName=PQD" name="eprints.official_url" />
<meta content="Ail-Ali T. Swain SM, Reid JB, Sun TP, Kamiya Y (1997) The LS locus of pea encodes the gibberellin biosynthesis enzvrne I?llt-kaurene synthase A. Plant J 11: 443-454 . --
Altschul SF, Madden TL, Schaffer AA, Zhang JH, Zhang Z, Miller W, Lipman OJ (1997) Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res 25: 3389-3402
Ausubel FM, Brent R, Kingston RE, Moore 00, Seidman JG, Smith JG, Strohl K (1994) Current Protocols in Molecular Biology 1. Wiley Interscience,
New York
Beveridge CA, Murfet IC (11;)96) The gigas mutant in pea is deficient in the floral stimulus. Physiol Plant 96: 637-645
Bishop GJ, Nomura T, Yokota T, Harrison K, Noguchi T, Fujioka S, Takatsuto 5, Jones JOG, Kamiya Y (l<:jI;l9) The tomato DWARF enzyme catalyses C-6 oxidation in brassinosteroid biosynthesis. Proc Natl Acad Sci USA 96: 1761-1766
Brown ,WS (1996) Arabidopsi« intron mutations and pre-mRNA splicing. Plant J 10: 771-780
Choe SW, Dilkes BP, Fujioka S, Takatsuto 5, Sakurai A, Feldmann KA (1998) The DWF4 gene of Arabidopsis encodes a cytochrome P450 that mediates multiple 22 alpha-hydroxylation steps in brassinosteroid biosynthesis.
Plant Cell 10: 231-243
Cullin C, Pompon 0 (1988) Synthesis of functional mouse cytochromes P-450 PI and chimeric P-450 P3-1 in the yeast Saccharomyces cereuisiae. Gene 65: 203-217
Frohman MA, Dush MK, Martin GR (1988) Rapid production of full-length cDN.'\s from rare transcripts: amplification using a single gene-specific oligonucleotide primer. Pror Natl Acad Sci USA 85: R998-9002
Frydman VM, Gaskin P, MacMillan J (1974) Qualitative and quantitative analyses of gibberellins throughout seed maturation in Pisun:saiioum CV. Progress no.9. Planta 118: 123-132
Gaskin P, MacMillan J (l9'l1) GC-MS of the gibberellins and related compounds:
methodology and a library of spectra. University of Bristol, Cantock's Enterprises, Bristol, UK
GroJlelindemann E, Lewis MJ, Hedden P, Graebe JE (1992) Gibberellin biosynthesis from gibberellin A12-aldehyde in a cell-free system from germinating barley (Hordeun. "u/sarl.' L, Cv Himalaya) embryos. Plante
188: 252-257
Gutierrez RA, Macintosh GC, Green PJ (1999) Current perspectives on mRNA stability in plants: multiple levels and mechanisms of control. Trerds Plant Sci 4: 429-438
Hedden P (1997)The oxidases of gibberellin biosynthesis: their function and mechanism. Physiol Plant 101: 709-719
Hedden P, Graebe JE, Beale MH, Gaskin P, MacMillan J (1984) The biosynthesis of l Zo-hydroxylated gibberellins in a cell-free system from Cucurbita maxima endosperm. Phytochemistry 23: 569-574
Hedden P, Kamiya Y (1997) Gibberellin biosynthesis: enzymes, genes and their regulation. Annu Rev Plant Physiol Plant Mol BioI 48: 431--460 Hedden P, Phillips AL (2000) Gibberellin metabolism: new insights revealed
by the genes. Trends Plant Sci 5: 523-530
343
Davidson et al.
Hedden P, Phillips AL, Rojas MC, Carrera E, Tudzynski B (2002) Gibberellin
biosynthesis in plants and fungi: ,1 case 'of convergent evolution? J Plant Growth Regul 20: 319-331
Hedden P, Proebsting WM (l9'19) Genetic analysis of gibberellin biosynthesis.
Pl,mt Physiol 119: 365-370
Helliwell CA, Chandler PM, Poole A, Dennis ES, Peacock WJ (2001) The CYP88A cytochrome P-l,:iO, 1.'11f-kaurenoic acid oxidase, catalyzes three steps of the gibberellin biosynthesis pathway. Proc \Jat! Acari Sci USA 98: 2065-2070
Helliwell CA, Olive MR, Gebbie L, Forster R, Peacock WJ, Dennis ES (2000) Isolation of an "I/f-kaurenl' oxidase cDN..\ from Cucurbita maxima, Aust J Plant Physiol27: 11-l1-1H9 Helliwell CA, Poole A, Peacock WJ, Dennis ES (1999) Aml1idopsis entkaurcne
oxidase catalyzes three steps of gibberellin biosynthesis. Plant Physiol 119: 507-510 Helliwell CA, Sheldon cc.Olive MR, Walker AR, Zeevaart JAD, Peacock WJ, Dennis ES (1998) Cloning of the Arabidopsis cnt-kaurene oxidase gene GA3. Proc N,ltl Acad Sci USA 95: 9019-902-l Hooley R (199-l) Gibberellins: perception, transduction and responses. Plant \1101 Bioi 26: 1529-1555
Imai M, Shimada H, Watanabe Y, Matsushima-Hibiya Y, Makino R, Koga H, Horiuchi T, Ishimura Y (1989) Uncoupling of the cytochrome P--l50cam monooxvgenase reaction by a single mutation, threonine-252 to alanine or valine: possible role of the hydroxy amino acid in oxygen activation. Proc Natl Acad Sci USA 86: 7823-7827
Ingram TJ, Reid JB (1987) Internode length in Pisutn): Cenc /III may block gibberellin synthesis between l'l/t-7-alplw-hydroxykaurl'noic acid and gibberellin
A12-aldehyde. Plant Physiol 83: 10-18-1053
Ingram TJ, Reid JB, MacMillian J (1986) The quantitative relationship between gibberellin A1 and internode growth in Pi511111 satitnnn L. Planta
168: -lH-l20 Ingram TJ, Reid JB, Murfet IC, Gaskin P, Willis CL, MacMillian J (lY8-l) Internode length in Pisuiu, Planta ]60: -l55-l63
Kalb VF, Loper JC (1988) Proteins from eight eukaryotic cytochrome P--l50 families share ,1 segmented region of sequence similarity. Proc Natl Acad Sci USA 85: 7221-7225
Kamiya Y, Graebe JE (1983) The biosynthesis of all major pea gibberellins in a cell-free system from Pi~III/I satiuutn. Phytochemistry 22: 681-689
Lange T (14lJ7) Cloning gibberellin dioxygcnase genes from pumpkin endosperm
by heterologous expression off enzyme activities in Escherichia coli. Proc Natl Acad Sci USA 94: 6553-6558
Lester DR, MacKenzie-Hose AK, Davies PI, Ross JJ, Reid JB (1999,1) The influence of the nu1l1e-'2 mutation on gibberellin levels in developing pea seeds. Plant Growth Regul 27: 83-89 lester DR, Ross JJ, Davies PJ, Reid J8 (1997) Mendels stem length gene (Ll') encodes a gibberellin 3-beta-hydroxylase. Plant Cell 9: ]435-1443 lester DR, Ross JI, Smith JJ, Elliotl RC, Reid JB (1999b) Gibberellin 2-oxidation and the SU..: gene of Pi5//111 :;nti"1I111. PI,1l1t I 19: 65-73 MacMillan J (1997) Biosynthesis of the gibbl'rellin plant hormones. f'\at Prod Rep 14: 221-2·B
Martin DN, Proebsting WM, Hedden P (1997) Mendel's dwarfing gene: cDNAs from the L,' alleles and function of the expressed .proteins. Proc Natl Acad Sci USA 94: 8907-8911 Martin ON, Proebsting WM, Hedden P (1999) The SLENDER gene of pea encodes .1 gibberellin 2-oxidase. Plant Physiol 121: 775-7R1 Phillips AL, Ward DA, Uknes S, Appleford NEJ, lange T, HUllly AK, Gaskin P, Graebe JE, Hedden P (1995) Isolation and expression of 3 gibberellin 20-oxidase cDl\'A clones from Am/Jidop~i~. 1)lant Physiol ]08: 1049-1057
Pompon D, Louerat B, Bronine A, Urban P (1996) Yeast expression of animal and plant P-l50s in optimized redox environments, Methods Enzymol 272: 51-6-l
Poole AT, Ross JJ,Lawrence Nl, Reid J8 (1995) Identification of gibberellin A~ in Pisun: ,at;1'II/II L. and the effects of applied gibb('reJlins A9 , A~, A~ and A, 011 the lc mutant. PI,1l1t Growth Regul ]6: 257-262
Potts WC (1986) Gibberellins in light-grown shoots of Pis/Oll satitnnn L. and the influence of reproductive development. Plant Cell Physiol 27: 997-1003 Potts we, Reid J8 (1983) Internode len~th in Pisum: Ill. The effect and interaction of the Na/fla and Lc;71' gene differences on endogenous gibberellin-like substances. Physiol Plant 57: 448-454
Poulos TL, Finzel BC, Gunsalus IC, Wagner GC, Kraut J (19R3) The 2.6-A crysta I structure of Pseudomonas putida cytochrome P-450. J Bioi Chern
260: 16122-lh130
Proebsting WM, Hedden P, Lewis MJ, Croker SJ, Proebsting LN (1992) Gibberellin concentration and transport in genetic lines of pea: effects of grafting. Plant Physiol 100: 1334-13hO
Reid J8 (1986a) Gibberellin mutants. ln PJ King, AD Blonstein. eds, Plant Gene Research: 1\ Genetic Approach to Plant Biochemistry, Vol 3. Springer-Verlag, New York, pp 1-34
Reid J8 (1986b) Internode length in PiSIIlI/. Three further loci, Iii, {~ and Ik. Ann 130:: 57: 577-592
Reid JB, Murfet IC, Potts WC (1983) Internode Length in PiS/IIII: II. Additional
information on the relationship and action of loci u: La,Cry. Na. and Lm. J Exp Bot 34: 3-l9-364
Reid JB, Ross JJ(1993) A mutant based approach, using PiS/11II saticum, to understand plant growth. Int J Plant Sci 154: 22-34 Reid JB, Ross H,Swain SM (1992) Internode length in PiSIII1I. A new, slender mutant with elevated levels of C19 gibberellins. Planta 188: 462-1-67
Rojas MC, Hedden P, Gaskin P, Tudzynki B (2001) The P-lSO-1 gene of Cibbcretl« f/ljikl/roi encodes" multifunctional enzyme in gibberellin biosynthesis.
Proc Natl Acad Sci USA 98: 5838-58-lJ
Ross H, Murfet IC, Reid JB (l':lY7) Gibberellin mutants. Physiol Plant 100: 550-56(]
Ross JJ, Reid JB, Swain SM (1993) Control of stem elongation by gibberellin AI: evidence from genetic studies including the slender mutant sln, Aust J Plant I'hysiol 20: 585-599
Ross H, Rl!id JB, Swain SM, Hasan 0, Poole AT, Hedden P, Willis ct, (1995) Genetic regulation of gibberellin deactivation in Pi~II/11. Plant J 7: 513-523
Sekimoto H, Seo M, Dohmae N, Takio K, Kamiya Y, Koshiba T (IY97) Cloning and molecular characterization of plant aldehyde oxidase. J Bioi Chem 2,'2: 13280-15285
Sekimoto H, Seo M, Kawakami N, Komano T, Desloire S, Liotenberg S, Marion-roll A, Caboche M, Kamiya Y, Koshiba T (1998) Molecular cloning and characterization of aldehyde oxidases in Arabidopsis tluilinna. Plant Cell Physiol 39: 433-442
Seo M, Akaba S, Oritani T, Delarue M, Bellini C. Caboche M, Koshiba T (1998) Higher activity of an aldehyde oxidase in the auxin-overproducing superroctl mutant of Aml>idopsis tltaliana. Plant Physiol 116: 687-693
Sponsel VM, Schmidt FW, Porter SG, Nakayama M, Kohlstruk S, Estelle M (1997) Characterization of new gibberellin-responsive sernidwarf mutants
of Arabidops;«. Plant Physiol115: 1009-1020
Swain SM, Reid J8, Kamiya Y (1997) Cibberellins are required for embryo growth end seed development in pea. Plant J 12: 1329-1338
SwainSM,ReidJB,RossJJ(1943)Seeddevelopmentin PisII111:The lit;allele reduces gibberellin levels in developing seeds, and increases seed abortion.
Plantil 19]: 482-488
Swain SM, Ross JJ, Reid JB, Kamiya Y (1995) GibberelJins and pea seed development: expression of the 11/', /5 and h_,·oN mutations. Planta ]95: 42h-433
Swofford DL (1999) PAUP·. Phylogenetic analysis using parsimony ("and other methods). Version 4. Sinauer Associates, Sunderland, MA Szekeres M, Nemeth K, KonczKalman Z, Mathur J, Kauschmann A, Altmann
T, Redei GP, Nagy F, Schell J, Koncz C (1996) Brassinosteroids rescue the deficiency of CYP90, a cytochrome P450, controlling cell elongation and de-etiolation in Arabidopsis. Cell 85: 171-182 Talon M, Koornncef M, Zeevaart JAD (1990) Endogenous gibberellins in Arabidop~is thaliana and possible steps blocked in the biosynthetic pathways
of the sernidwarf ga4 and gaS mutants. Proc Katl Acad Sci USA 87: 7983-7987 Urban P, Mignotte C, Kazmaier M, Delorme F, Pompon D (1497) Cloning, yeast expression, and characterization of the coupling of two distantly related Ilrabid0l'"is thnliana NADPH-cytochrome P450 reductascs with P450 CYP73A5. J BioI Chern 272: 19176-19186 Winkler RG, Helentjaris T (1995) The maize Dwar}3 gene encodes it cytochrome
f450-mediated early step in gibberellin biosynthesis. Plant Cell 7: 1307-1317
Xu YL, li L, Wu KQ, Peeters AJM, Gage DA, Zeevaart JAD (1995) The GnS locus of Arnbid(lpsi~ tlmlian« encodes a multifunctional gibberellin 20oxidase:
molecular cloning and functional expression. I'roc Nat! Acad Sci USA 92: 6640-6644
Yaxley JR, Ross JJ, Sherriff LJ, Reid JB (2001) Gibberellin biosynthesis mutations and root development in pea. Plant Physiol 125: 627-633
" name="eprints.referencetext" />
<meta content="Davidson, Sandra E. and Elliott, Robert C. and Helliwell, Chris A. and Poole, Andrew T. and Reid, James B. (2003) The Pea Gene NA Encodes ent-Kaurenoic Acid Oxidase 1. Plant Physiology, 131 (1). pp. 335-344. ISSN 0032-0889" name="eprints.citation" />
<meta content="http://eprints.utas.edu.au/2365/1/thepea_geneNA.pdf" name="eprints.document_url" />
<link rel="schema.DC" href="http://purl.org/DC/elements/1.0/" />
<meta content="The Pea Gene NA Encodes ent-Kaurenoic Acid Oxidase 1" name="DC.title" />
<meta content="Davidson, Sandra E." name="DC.creator" />
<meta content="Elliott, Robert C." name="DC.creator" />
<meta content="Helliwell, Chris A." name="DC.creator" />
<meta content="Poole, Andrew T." name="DC.creator" />
<meta content="Reid, James B." name="DC.creator" />
<meta content="320000 Medical and Health Sciences" name="DC.subject" />
<meta content="The gibberellin (GA)-deficient dwarf na mutant in pea tPisum sativum) has severely reduced internode elongation, reduced root growth, and decreased leaflet size. However, the seeds develop normally. Two genes, PsKAOl and PsKA02, encoding cytochrome P450 monooxygenases of the subfamily CYP88A were isolated. Both PsKAOl and PsKA02 had cllt-kaurenoic acid oxidase (KAO) activity, catalyzing the three steps of the GA biosynthetic 'pathway from ellt-kaurenoic acid to GA12 when expressed in yeast (Saccharolllyces cereuisiaes. In addition to the intermediates cnt-7n-hydroxykaurenoic acid and GAn-aldehyde, some additional products of the pea KAO activity wen! detected, induding ent-60',70'-dihydroxykaurenoic acid and 7(:l-hydroxykaurenolide. The NA gene encodes PsKA01, because in two independent mutant alleles, na-t and na-L, PsKAOl had altered sequences and the five-base deletion in PsKAOl associated with the tl17-1 allele cosegregated with the dwarf 1117 phenotype. P5KAOI was expressed in the stem, apical bud, leaf, pod, and root, organs in which GA levels have previously been shown to be reduced in IIIl plants. PsKA02 was expressed only in seeds and this may explain the normal seed development and normal GA biosynthesis in seeds of lla plants." name="DC.description" />
<meta content="2003" name="DC.date" />
<meta content="Article" name="DC.type" />
<meta content="PeerReviewed" name="DC.type" />
<meta content="application/pdf" name="DC.format" />
<meta content="http://eprints.utas.edu.au/2365/1/thepea_geneNA.pdf" name="DC.identifier" />
<meta content="http://proquest.umi.com/pqdweb?did=288001151&sid=1&Fmt=4&clientId=20931&RQT=309&VName=PQD" name="DC.relation" />
<meta content="Davidson, Sandra E. and Elliott, Robert C. and Helliwell, Chris A. and Poole, Andrew T. and Reid, James B. (2003) The Pea Gene NA Encodes ent-Kaurenoic Acid Oxidase 1. Plant Physiology, 131 (1). pp. 335-344. ISSN 0032-0889" name="DC.identifier" />
<meta content="http://eprints.utas.edu.au/2365/" name="DC.relation" />
<link rel="alternate" href="http://eprints.utas.edu.au/cgi/export/2365/BibTeX/epprod-eprint-2365.bib" title="BibTeX" type="text/plain" />
<link rel="alternate" href="http://eprints.utas.edu.au/cgi/export/2365/ContextObject/epprod-eprint-2365.xml" title="OpenURL ContextObject" type="text/xml" />
<link rel="alternate" href="http://eprints.utas.edu.au/cgi/export/2365/ContextObject::Dissertation/epprod-eprint-2365.xml" title="OpenURL Dissertation" type="text/xml" />
<link rel="alternate" href="http://eprints.utas.edu.au/cgi/export/2365/ContextObject::Journal/epprod-eprint-2365.xml" title="OpenURL Journal" type="text/xml" />
<link rel="alternate" href="http://eprints.utas.edu.au/cgi/export/2365/DC/epprod-eprint-2365.txt" title="Dublin Core" type="text/plain" />
<link rel="alternate" href="http://eprints.utas.edu.au/cgi/export/2365/DIDL/epprod-eprint-2365.xml" title="DIDL" type="text/xml" />
<link rel="alternate" href="http://eprints.utas.edu.au/cgi/export/2365/EndNote/epprod-eprint-2365.enw" title="EndNote" type="text/plain" />
<link rel="alternate" href="http://eprints.utas.edu.au/cgi/export/2365/HTML/epprod-eprint-2365.html" title="HTML Citation" type="text/html; charset=utf-8" />
<link rel="alternate" href="http://eprints.utas.edu.au/cgi/export/2365/METS/epprod-eprint-2365.xml" title="METS" type="text/xml" />
<link rel="alternate" href="http://eprints.utas.edu.au/cgi/export/2365/MODS/epprod-eprint-2365.xml" title="MODS" type="text/xml" />
<link rel="alternate" href="http://eprints.utas.edu.au/cgi/export/2365/RIS/epprod-eprint-2365.ris" title="Reference Manager" type="text/plain" />
<link rel="alternate" href="http://eprints.utas.edu.au/cgi/export/2365/Refer/epprod-eprint-2365.refer" title="Refer" type="text/plain" />
<link rel="alternate" href="http://eprints.utas.edu.au/cgi/export/2365/Simple/epprod-eprint-2365text" title="Simple Metadata" type="text/plain" />
<link rel="alternate" href="http://eprints.utas.edu.au/cgi/export/2365/Text/epprod-eprint-2365.txt" title="ASCII Citation" type="text/plain; charset=utf-8" />
<link rel="alternate" href="http://eprints.utas.edu.au/cgi/export/2365/XML/epprod-eprint-2365.xml" title="EP3 XML" type="text/xml" />
</head>
<body bgcolor="#ffffff" text="#000000" onLoad="loadRoutine(); MM_preloadImages('images/eprints/ePrints_banner_r5_c5_f2.gif','images/eprints/ePrints_banner_r5_c7_f2.gif','images/eprints/ePrints_banner_r5_c8_f2.gif','images/eprints/ePrints_banner_r5_c9_f2.gif','images/eprints/ePrints_banner_r5_c10_f2.gif','images/eprints/ePrints_banner_r5_c11_f2.gif','images/eprints/ePrints_banner_r6_c4_f2.gif')">
<div class="ep_noprint"><noscript><style type="text/css">@import url(http://eprints.utas.edu.au/style/nojs.css);</style></noscript></div>
<table width="795" border="0" cellspacing="0" cellpadding="0">
<tr>
<td><script language="JavaScript1.2">mmLoadMenus();</script>
<table border="0" cellpadding="0" cellspacing="0" width="795">
<!-- fwtable fwsrc="eprints_banner_final2.png" fwbase="ePrints_banner.gif" fwstyle="Dreamweaver" fwdocid = "1249563342" fwnested="0" -->
<tr>
<td><img src="/images/eprints/spacer.gif" width="32" height="1" border="0" alt="" /></td>
<td><img src="/images/eprints/spacer.gif" width="104" height="1" border="0" alt="" /></td>
<td><img src="/images/eprints/spacer.gif" width="44" height="1" border="0" alt="" /></td>
<td><img src="/images/eprints/spacer.gif" width="105" height="1" border="0" alt="" /></td>
<td><img src="/images/eprints/spacer.gif" width="41" height="1" border="0" alt="" /></td>
<td><img src="/images/eprints/spacer.gif" width="16" height="1" border="0" alt="" /></td>
<td><img src="/images/eprints/spacer.gif" width="68" height="1" border="0" alt="" /></td>
<td><img src="/images/eprints/spacer.gif" width="68" height="1" border="0" alt="" /></td>
<td><img src="/images/eprints/spacer.gif" width="68" height="1" border="0" alt="" /></td>
<td><img src="/images/eprints/spacer.gif" width="82" height="1" border="0" alt="" /></td>
<td><img src="/images/eprints/spacer.gif" width="69" height="1" border="0" alt="" /></td>
<td><img src="/images/eprints/spacer.gif" width="98" height="1" border="0" alt="" /></td>
<td><img src="/images/eprints/spacer.gif" width="1" height="1" border="0" alt="" /></td>
</tr>
<tr>
<td colspan="12"><img name="ePrints_banner_r1_c1" src="/images/eprints/ePrints_banner_r1_c1.gif" width="795" height="10" border="0" alt="" /></td>
<td><img src="/images/eprints/spacer.gif" width="1" height="10" border="0" alt="" /></td>
</tr>
<tr>
<td rowspan="6"><img name="ePrints_banner_r2_c1" src="/images/eprints/ePrints_banner_r2_c1.gif" width="32" height="118" border="0" alt="" /></td>
<td rowspan="5"><a href="http://www.utas.edu.au/"><img name="ePrints_banner_r2_c2" src="/images/eprints/ePrints_banner_r2_c2.gif" width="104" height="103" border="0" alt="" /></a></td>
<td colspan="10"><img name="ePrints_banner_r2_c3" src="/images/eprints/ePrints_banner_r2_c3.gif" width="659" height="41" border="0" alt="" /></td>
<td><img src="/images/eprints/spacer.gif" width="1" height="41" border="0" alt="" /></td>
</tr>
<tr>
<td colspan="3"><a href="http://eprints.utas.edu.au/"><img name="ePrints_banner_r3_c3" src="/images/eprints/ePrints_banner_r3_c3.gif" width="190" height="31" border="0" alt="" /></a></td>
<td rowspan="2" colspan="7"><img name="ePrints_banner_r3_c6" src="/images/eprints/ePrints_banner_r3_c6.gif" width="469" height="37" border="0" alt="" /></td>
<td><img src="/images/eprints/spacer.gif" width="1" height="31" border="0" alt="" /></td>
</tr>
<tr>
<td colspan="3"><img name="ePrints_banner_r4_c3" src="/images/eprints/ePrints_banner_r4_c3.gif" width="190" height="6" border="0" alt="" /></td>
<td><img src="/images/eprints/spacer.gif" width="1" height="6" border="0" alt="" /></td>
</tr>
<tr>
<td colspan="2"><img name="ePrints_banner_r5_c3" src="/images/eprints/ePrints_banner_r5_c3.gif" width="149" height="1" border="0" alt="" /></td>
<td rowspan="2" colspan="2"><a href="/information.html" onMouseOut="MM_swapImgRestore();MM_startTimeout()" onMouseOver="MM_showMenu(window.mm_menu_0821132634_0,0,25,null,'ePrints_banner_r5_c5');MM_swapImage('ePrints_banner_r5_c5','','/images/eprints/ePrints_banner_r5_c5_f2.gif',1);"><img name="ePrints_banner_r5_c5" src="/images/eprints/ePrints_banner_r5_c5.gif" width="57" height="25" border="0" alt="About" /></a></td>
<td rowspan="2"><a href="/view/" onMouseOut="MM_swapImgRestore();MM_startTimeout()" onMouseOver="MM_showMenu(window.mm_menu_0821133021_1,0,25,null,'ePrints_banner_r5_c7');MM_swapImage('ePrints_banner_r5_c7','','/images/eprints/ePrints_banner_r5_c7_f2.gif',1);"><img name="ePrints_banner_r5_c7" src="/images/eprints/ePrints_banner_r5_c7.gif" width="68" height="25" border="0" alt="Browse" /></a></td>
<td rowspan="2"><a href="/perl/search/simple" onMouseOut="MM_swapImgRestore();MM_startTimeout()" onMouseOver="MM_showMenu(window.mm_menu_0821133201_2,0,25,null,'ePrints_banner_r5_c8');MM_swapImage('ePrints_banner_r5_c8','','/images/eprints/ePrints_banner_r5_c8_f2.gif',1);"><img name="ePrints_banner_r5_c8" src="/images/eprints/ePrints_banner_r5_c8.gif" width="68" height="25" border="0" alt="Search" /></a></td>
<td rowspan="2"><a href="/perl/register" onMouseOut="MM_swapImgRestore();MM_startTimeout();" onMouseOver="MM_showMenu(window.mm_menu_1018171924_3,0,25,null,'ePrints_banner_r5_c9');MM_swapImage('ePrints_banner_r5_c9','','/images/eprints/ePrints_banner_r5_c9_f2.gif',1);"><img name="ePrints_banner_r5_c9" src="/images/eprints/ePrints_banner_r5_c9.gif" width="68" height="25" border="0" alt="register" /></a></td>
<td rowspan="2"><a href="/perl/users/home" onMouseOut="MM_swapImgRestore();MM_startTimeout()" onMouseOver="MM_showMenu(window.mm_menu_0821133422_4,0,25,null,'ePrints_banner_r5_c10');MM_swapImage('ePrints_banner_r5_c10','','/images/eprints/ePrints_banner_r5_c10_f2.gif',1);"><img name="ePrints_banner_r5_c10" src="/images/eprints/ePrints_banner_r5_c10.gif" width="82" height="25" border="0" alt="user area" /></a></td>
<td rowspan="2"><a href="/help/" onMouseOut="MM_swapImgRestore();MM_startTimeout()" onMouseOver="MM_showMenu(window.mm_menu_0821133514_5,0,25,null,'ePrints_banner_r5_c11');MM_swapImage('ePrints_banner_r5_c11','','/images/eprints/ePrints_banner_r5_c11_f2.gif',1);"><img name="ePrints_banner_r5_c11" src="/images/eprints/ePrints_banner_r5_c11.gif" width="69" height="25" border="0" alt="Help" /></a></td>
<td rowspan="3" colspan="4"><img name="ePrints_banner_r5_c12" src="/images/eprints/ePrints_banner_r5_c12.gif" width="98" height="40" border="0" alt="" /></td>
<td><img src="/images/eprints/spacer.gif" width="1" height="1" border="0" alt="" /></td>
</tr>
<tr>
<td rowspan="2"><img name="ePrints_banner_r6_c3" src="/images/eprints/ePrints_banner_r6_c3.gif" width="44" height="39" border="0" alt="ePrints home" /></td>
<td><a href="/" onMouseOut="MM_swapImgRestore()" onMouseOver="MM_swapImage('ePrints_banner_r6_c4','','/images/eprints/ePrints_banner_r6_c4_f2.gif',1);"><img name="ePrints_banner_r6_c4" src="/images/eprints/ePrints_banner_r6_c4.gif" width="105" height="24" border="0" alt="ePrints home" /></a></td>
<td><img src="/images/eprints/spacer.gif" width="1" height="24" border="0" alt="" /></td>
</tr>
<tr>
<td><img name="ePrints_banner_r7_c2" src="/images/eprints/ePrints_banner_r7_c2.gif" width="104" height="15" border="0" alt="" /></td>
<td colspan="8"><img name="ePrints_banner_r7_c4" src="/images/eprints/ePrints_banner_r7_c4.gif" width="517" height="15" border="0" alt="" /></td>
<td><img src="/images/eprints/spacer.gif" width="1" height="15" border="0" alt="" /></td>
</tr>
</table></td>
</tr>
<tr><td><table width="100%" style="font-size: 90%; border: solid 1px #ccc; padding: 3px"><tr>
<td align="left"><a href="http://eprints.utas.edu.au/cgi/users/home">Login</a> | <a href="http://eprints.utas.edu.au/cgi/register">Create Account</a></td>
<td align="right" style="white-space: nowrap">
<form method="get" accept-charset="utf-8" action="http://eprints.utas.edu.au/cgi/search" style="display:inline">
<input class="ep_tm_searchbarbox" size="20" type="text" name="q" />
<input class="ep_tm_searchbarbutton" value="Search" type="submit" name="_action_search" />
<input type="hidden" name="_order" value="bytitle" />
<input type="hidden" name="basic_srchtype" value="ALL" />
<input type="hidden" name="_satisfyall" value="ALL" />
</form>
</td>
</tr></table></td></tr>
<tr>
<td class="toplinks"><!-- InstanceBeginEditable name="content" -->
<div align="center">
<table width="720" class="ep_tm_main"><tr><td align="left">
<h1 class="ep_tm_pagetitle">The Pea Gene NA Encodes ent-Kaurenoic Acid Oxidase 1</h1>
<p style="margin-bottom: 1em" class="not_ep_block"><span class="person_name">Davidson, Sandra E.</span> and <span class="person_name">Elliott, Robert C.</span> and <span class="person_name">Helliwell, Chris A.</span> and <span class="person_name">Poole, Andrew T.</span> and <span class="person_name">Reid, James B.</span> (2003) <xhtml:em>The Pea Gene NA Encodes ent-Kaurenoic Acid Oxidase 1.</xhtml:em> Plant Physiology, 131 (1). pp. 335-344. ISSN 0032-0889</p><p style="margin-bottom: 1em" class="not_ep_block"></p><table style="margin-bottom: 1em" class="not_ep_block"><tr><td valign="top" style="text-align:center"><a href="http://eprints.utas.edu.au/2365/1/thepea_geneNA.pdf"><img alt="[img]" src="http://eprints.utas.edu.au/style/images/fileicons/application_pdf.png" class="ep_doc_icon" border="0" /></a></td><td valign="top"><a href="http://eprints.utas.edu.au/2365/1/thepea_geneNA.pdf"><span class="ep_document_citation">PDF</span></a> - Full text restricted - Requires a PDF viewer<br />3367Kb</td><td><form method="get" accept-charset="utf-8" action="http://eprints.utas.edu.au/cgi/request_doc"><input accept-charset="utf-8" value="3049" name="docid" type="hidden" /><div class=""><input value="Request a copy" name="_action_null" class="ep_form_action_button" onclick="return EPJS_button_pushed( '_action_null' )" type="submit" /> </div></form></td></tr></table><p style="margin-bottom: 1em" class="not_ep_block">Official URL: <a href="http://proquest.umi.com/pqdweb?did=288001151&sid=1&Fmt=4&clientId=20931&RQT=309&VName=PQD">http://proquest.umi.com/pqdweb?did=288001151&sid=1&Fmt=4&clientId=20931&RQT=309&VName=PQD</a></p><div class="not_ep_block"><h2>Abstract</h2><p style="padding-bottom: 16px; text-align: left; margin: 1em auto 0em auto">The gibberellin (GA)-deficient dwarf na mutant in pea tPisum sativum) has severely reduced internode elongation, reduced root growth, and decreased leaflet size. However, the seeds develop normally. Two genes, PsKAOl and PsKA02, encoding cytochrome P450 monooxygenases of the subfamily CYP88A were isolated. Both PsKAOl and PsKA02 had cllt-kaurenoic acid oxidase (KAO) activity, catalyzing the three steps of the GA biosynthetic 'pathway from ellt-kaurenoic acid to GA12 when expressed in yeast (Saccharolllyces cereuisiaes. In addition to the intermediates cnt-7n-hydroxykaurenoic acid and GAn-aldehyde, some additional products of the pea KAO activity wen! detected, induding ent-60',70'-dihydroxykaurenoic acid and 7(:l-hydroxykaurenolide. The NA gene encodes PsKA01, because in two independent mutant alleles, na-t and na-L, PsKAOl had altered sequences and the five-base deletion in PsKAOl associated with the tl17-1 allele cosegregated with the dwarf 1117 phenotype. P5KAOI was expressed in the stem, apical bud, leaf, pod, and root, organs in which GA levels have previously been shown to be reduced in IIIl plants. PsKA02 was expressed only in seeds and this may explain the normal seed development and normal GA biosynthesis in seeds of lla plants.</p></div><table style="margin-bottom: 1em" cellpadding="3" class="not_ep_block" border="0"><tr><th valign="top" class="ep_row">Item Type:</th><td valign="top" class="ep_row">Article</td></tr><tr><th valign="top" class="ep_row">Subjects:</th><td valign="top" class="ep_row"><a href="http://eprints.utas.edu.au/view/subjects/320000.html">320000 Medical and Health Sciences</a></td></tr><tr><th valign="top" class="ep_row">ID Code:</th><td valign="top" class="ep_row">2365</td></tr><tr><th valign="top" class="ep_row">Deposited By:</th><td valign="top" class="ep_row"><span class="ep_name_citation"><span class="person_name">Scholarly Publications Librarian</span></span></td></tr><tr><th valign="top" class="ep_row">Deposited On:</th><td valign="top" class="ep_row">30 Oct 2007 14:59</td></tr><tr><th valign="top" class="ep_row">Last Modified:</th><td valign="top" class="ep_row">09 Jan 2008 02:30</td></tr><tr><th valign="top" class="ep_row">ePrint Statistics:</th><td valign="top" class="ep_row"><a target="ePrintStats" href="/es/index.php?action=show_detail_eprint;id=2365;">View statistics for this ePrint</a></td></tr></table><p align="right">Repository Staff Only: <a href="http://eprints.utas.edu.au/cgi/users/home?screen=EPrint::View&eprintid=2365">item control page</a></p>
</td></tr></table>
</div>
<!-- InstanceEndEditable --></td>
</tr>
<tr>
<td><!-- #BeginLibraryItem "/Library/footer_eprints.lbi" -->
<table width="795" border="0" align="left" cellpadding="0" class="footer">
<tr valign="top">
<td colspan="2"><div align="center"><a href="http://www.utas.edu.au">UTAS home</a> | <a href="http://www.utas.edu.au/library/">Library home</a> | <a href="/">ePrints home</a> | <a href="/contact.html">contact</a> | <a href="/information.html">about</a> | <a href="/view/">browse</a> | <a href="/perl/search/simple">search</a> | <a href="/perl/register">register</a> | <a href="/perl/users/home">user area</a> | <a href="/help/">help</a></div><br /></td>
</tr>
<tr><td colspan="2"><p><img src="/images/eprints/footerline.gif" width="100%" height="4" /></p></td></tr>
<tr valign="top">
<td width="68%" class="footer">Authorised by the University Librarian<br />
© University of Tasmania ABN 30 764 374 782<br />
<a href="http://www.utas.edu.au/cricos/">CRICOS Provider Code 00586B</a> | <a href="http://www.utas.edu.au/copyright/copyright_disclaimers.html">Copyright & Disclaimers</a> | <a href="http://www.utas.edu.au/accessibility/index.html">Accessibility</a> | <a href="http://eprints.utas.edu.au/feedback/">Site Feedback</a> </td>
<td width="32%"><div align="right">
<p align="right" class="NoPrint"><a href="http://www.utas.edu.au/"><img src="http://www.utas.edu.au/shared/logos/unioftasstrip.gif" alt="University of Tasmania Home Page" width="260" height="16" border="0" align="right" /></a></p>
<p align="right" class="NoPrint"><a href="http://www.utas.edu.au/"><br />
</a></p>
</div></td>
</tr>
<tr valign="top">
<td><p> </p></td>
<td><div align="right"><span class="NoPrint"><a href="http://www.eprints.org/software/"><img src="/images/eprintslogo.gif" alt="ePrints logo" width="77" height="29" border="0" align="bottom" /></a></span></div></td>
</tr>
</table>
<!-- #EndLibraryItem -->
<div align="center"></div></td>
</tr>
</table>
</body>
</html>
