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    <title>UTas ePrints - Ozonation of seawater improves the survival of larval southern rock lobster, Jasus edwardsii, in culture from egg to juvenile</title>
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    <meta content="Ritar, Arthur J." name="eprints.creators_name" />
<meta content="Smith, Greg G." name="eprints.creators_name" />
<meta content="Thomas, Craig W." name="eprints.creators_name" />
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<meta content="Phyllosoma larvae of the southern rock lobster, Jasus edwardsii, were cultured from egg to juvenile. For larvae reared from hatch to Stage III, survival was highest and bacterial contamination was lowest in seawater ozonated at low and moderate levels (400 and 500 mV oxidation-reduction potential, ORP). By contrast, at high ozonation (600 mV), all larvae suffered deformities at the moult to Stage II and terminally starved, while in unozonated water (about 300 mV), all larvae died at Stage III probably as a consequence of Vibrio bacteria profileration. In a second experiment between Stages VI to VIII, larval survival was highest in ozonated water that had been filtered through activated charcoal and coral sand, compared to ozonated water with no filtration or filtered only through activated charcoal. Ozonated water with the combined filtration was used subsequently but there were ongoing deformities, so the level was progressively reduced from 400 mV at Stage VIII to 330 mV at Stage X, at which time ozonation was discontinued. Larvae were then cultured in unozonated water to metamorphosis of eight pueruli at 377 to 437 days after hatch, of which two survived to juvenile. Ozonation was thus effective up to Stage IX in improving culture water to minimise bacterial disease without problems of larval deformities." name="eprints.abstract" />
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<meta content="Arimoto, M., Sato, J., Maruyama, K., Mimura, G., Furusawa, I. 1996. Effect of chemical and physical treatments on the inactivation of striped jack nervous necrosis virus (SJNNV). Aquaculture 143, 15-22. 
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Buchan, K.A.H., Martin-Robichaud, D.J., Benfey, T.J. 2005. Measurement of dissolved ozone in sea water: a comparison of methods. Aquacultural Engineering 33, 225-231.
Coman, G.J., Sellars, M.J., Morehead, D.T. 2005. Toxicity of ozone generated from different combinations of ozone concentration (C) and exposure time (T): a comparison of the relative effect of C and T on hatch rates of Penaeus (Marsupenaeus) japonicus. Aquaculture 244, 141-145.
Danald, D., Ure, J., Lightner, D. 1979. Preliminary results of ozone disinfection of seawater containing the potential shrimp pathogens Vibrio sp. and Fusarium solani. Ozone: Science and Engineering 1, 329-334. 
Franson, M.A. 1976. DPD colormetric method. Standard Methods for the Examination of Water and Wastewater. 14th ed. American Public Health Association, Washington, DC. pp. 332-334. 
Fukunaga, K., Suzuki, T., Hara, A., Takama, K. 1992. Effect of ozone on the activites of reactive oxygen scavenging enzymes in RBC of ozone exposed Japanese charr Salvelinus leucomaenis. Free Radical Research Communications 17, 327-333.
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Smith, E.G., Ritar, A.J., Carter, C.G., Dunstan, G.A., Brown, M.R. 2003a. Morphological and biochemical characteristics of phyllosoma after photothermal manipulation of reproduction in broodstock of the spiny lobster, Jasus edwardsii.  Aquaculture 220, 299-311.
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Wheatly, M.G. 1999. Calcium homeostasis in Crustacea: the evolving role of branchial, renal, digestive and hypodermal epithelia. Journal of Experimental Zoology 283, 620-640." name="eprints.referencetext" />
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    <h1 class="ep_tm_pagetitle">Ozonation of seawater improves the survival of larval southern rock lobster, Jasus edwardsii, in culture from egg to juvenile</h1>
    <p style="margin-bottom: 1em" class="not_ep_block"><span class="person_name">Ritar, Arthur J.</span> and <span class="person_name">Smith, Greg G.</span> and <span class="person_name">Thomas, Craig W.</span> (2006) <xhtml:em>Ozonation of seawater improves the survival of larval southern rock lobster, Jasus edwardsii, in culture from egg to juvenile.</xhtml:em> Aquaculture, 261 (3). pp. 1014-1025. ISSN 0044-8486</p><p style="margin-bottom: 1em" class="not_ep_block"></p><table style="margin-bottom: 1em" class="not_ep_block"><tr><td valign="top" style="text-align:center"><a onmouseover="EPJS_ShowPreview( event, 'doc_preview_653' );" href="http://eprints.utas.edu.au/642/1/ozone_dose_Aquaculture_E-Prints.pdf" onmouseout="EPJS_HidePreview( event, 'doc_preview_653' );"><img alt="[img]" src="http://eprints.utas.edu.au/style/images/fileicons/application_pdf.png" class="ep_doc_icon" border="0" /></a><div class="ep_preview" id="doc_preview_653"><table><tr><td><img alt="" src="http://eprints.utas.edu.au/642/thumbnails/1/preview.png" class="ep_preview_image" border="0" /><div class="ep_preview_title">Preview</div></td></tr></table></div></td><td valign="top"><a href="http://eprints.utas.edu.au/642/1/ozone_dose_Aquaculture_E-Prints.pdf"><span class="ep_document_citation">PDF (Author Version)</span></a> - Requires a PDF viewer<br />243Kb</td></tr><tr><td valign="top" style="text-align:center"><a href="http://eprints.utas.edu.au/642/2/ritar-ozonation-aquaculture-pubv.pdf"><img alt="[img]" src="http://eprints.utas.edu.au/style/images/fileicons/application_pdf.png" class="ep_doc_icon" border="0" /></a></td><td valign="top"><a href="http://eprints.utas.edu.au/642/2/ritar-ozonation-aquaculture-pubv.pdf"><span class="ep_document_citation">PDF</span></a> - Full text restricted - Requires a PDF viewer<br />500Kb</td></tr></table><p style="margin-bottom: 1em" class="not_ep_block">Official URL: <a href="http://dx.doi.org/10.1016/j.aquaculture.2006.08.035">http://dx.doi.org/10.1016/j.aquaculture.2006.08.035</a></p><div class="not_ep_block"><h2>Abstract</h2><p style="padding-bottom: 16px; text-align: left; margin: 1em auto 0em auto">Phyllosoma larvae of the southern rock lobster, Jasus edwardsii, were cultured from egg to juvenile. For larvae reared from hatch to Stage III, survival was highest and bacterial contamination was lowest in seawater ozonated at low and moderate levels (400 and 500 mV oxidation-reduction potential, ORP). By contrast, at high ozonation (600 mV), all larvae suffered deformities at the moult to Stage II and terminally starved, while in unozonated water (about 300 mV), all larvae died at Stage III probably as a consequence of Vibrio bacteria profileration. In a second experiment between Stages VI to VIII, larval survival was highest in ozonated water that had been filtered through activated charcoal and coral sand, compared to ozonated water with no filtration or filtered only through activated charcoal. Ozonated water with the combined filtration was used subsequently but there were ongoing deformities, so the level was progressively reduced from 400 mV at Stage VIII to 330 mV at Stage X, at which time ozonation was discontinued. Larvae were then cultured in unozonated water to metamorphosis of eight pueruli at 377 to 437 days after hatch, of which two survived to juvenile. Ozonation was thus effective up to Stage IX in improving culture water to minimise bacterial disease without problems of larval deformities.</p></div><table style="margin-bottom: 1em" cellpadding="3" class="not_ep_block" border="0"><tr><th valign="top" class="ep_row">Item Type:</th><td valign="top" class="ep_row">Article</td></tr><tr><th valign="top" class="ep_row">Additional Information:</th><td valign="top" class="ep_row">The definitive version is available online at http://www.elsevier.com/locate/aquaculture</td></tr><tr><th valign="top" class="ep_row">Keywords:</th><td valign="top" class="ep_row">Phyllosoma, culture, ozone, microbiology, Vibrio</td></tr><tr><th valign="top" class="ep_row">Subjects:</th><td valign="top" class="ep_row"><a href="http://eprints.utas.edu.au/view/subjects/300799.html">300000 Agricultural, Veterinary and Environmental Sciences &gt; 300700 Fisheries Sciences &gt; 300799 Fisheries Sciences not elsewhere classified</a><br /><a href="http://eprints.utas.edu.au/view/subjects/300703.html">300000 Agricultural, Veterinary and Environmental Sciences &gt; 300700 Fisheries Sciences &gt; 300703 Aquaculture</a></td></tr><tr><th valign="top" class="ep_row">ID Code:</th><td valign="top" class="ep_row">642</td></tr><tr><th valign="top" class="ep_row">Deposited By:</th><td valign="top" class="ep_row"><span class="ep_name_citation"><span class="person_name">Dr Arthur J Ritar</span></span></td></tr><tr><th valign="top" class="ep_row">Deposited On:</th><td valign="top" class="ep_row">24 Jan 2007</td></tr><tr><th valign="top" class="ep_row">Last Modified:</th><td valign="top" class="ep_row">09 Jan 2008 02:30</td></tr><tr><th valign="top" class="ep_row">ePrint Statistics:</th><td valign="top" class="ep_row"><a target="ePrintStats" href="/es/index.php?action=show_detail_eprint;id=642;">View statistics for this ePrint</a></td></tr></table><p align="right">Repository Staff Only: <a href="http://eprints.utas.edu.au/cgi/users/home?screen=EPrint::View&amp;eprintid=642">item control page</a></p>
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