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<title>UTas ePrints - Oral administration of type II collagen suppresses pro-inflammatory mediator production by synoviocytes in rats with adjuvant arthritis</title>
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<meta content="Ding, Changhai" name="eprints.creators_name" />
<meta content="Li, Qin" name="eprints.creators_name" />
<meta content="Xiong, Z.Y." name="eprints.creators_name" />
<meta content="Zhou, A.W." name="eprints.creators_name" />
<meta content="Jones, Graeme" name="eprints.creators_name" />
<meta content="Xu, S.Y." name="eprints.creators_name" />
<meta content="chding@utas.edu.au" name="eprints.creators_id" />
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<meta content="2007-04-30" name="eprints.datestamp" />
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<meta content="Oral administration of type II collagen suppresses pro-inflammatory mediator production by synoviocytes in rats with adjuvant arthritis
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<meta content="320503" name="eprints.subjects" />
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<meta content="type II collagen, inflammatory mediator, oral tolerance, arthritis " name="eprints.keywords" />
<meta content="The definitive version is available at www.blackwell-synergy.com. Visit - http://www.blackwell-synergy.com/doi/abs/10.1046/j.1365-2249.2003.02167.x" name="eprints.note" />
<meta content="The objective of this study was to investigate the effect of the oral administration of type II collagen (CII) on pro-inflammatory mediator production by synoviocytes in rats with adjuvant arthritis (AA). Sprague-Dawley rats were fed with bovine CII either before immunization with Complete Freund's adjuvant (CFA) or after initiation of arthritis. Hind paw secondary swelling was measured and synoviocytes were harvested. Sera from portal vein of oral tolerized rats were collected and in vitro synoviocytes culture or synoviocytes-Peyer's Patches (PP) cells coculture system were developed. Interleukin (IL)-1 activity was measured by a mouse thymocyte activation assayed by MTT dye reduction and tumour necrosis factor (TNF) activity was measured by an L929 cytotoxicity bioassay. Nitric oxide (NO) and malondialdehyde (MDA) levels were measured by biochemical methods. We found that feeding with CII (5, 50 and 500 ug/kg) for 7 days before immunization significantly suppressed hind paw secondary swelling measured at day 16, 20, 24 and 28 (all P< 0.01) and pro-inflammatory mediator (IL-1, TNF, NO and MDA) production by synoviocytes (all P< 0.01) in rats with AA. Feeding with CII (5, 50 and 500 ug/kg) for 7 days after initiation of arthritis had a similar effect. CII (1, 10, 100 ug/ml) had no effect on IL-1 and TNF production by synoviocytes in vitro, but CII 10 ug/ml suppressed IL-1 and TNF production by synoviocytes-PP cells coculture system (P < 0.01), which was antagonized by anti-TGF-beta antibody (10 ug/ml) (P < 0.01). Portal serum (1 : 10) from oral tolerized rats suppressed IL-1 and TNF production by synoviocytes (P < 0.01), which was also antagonized by anti-TGF-beta antibody (10 ug/ml) (P < 0.01). We conclude that oral administration of CII had prophylactic and therapeutic effects on AA and over-production of IL-1, TNF, NO and MDA by synoviocytes was suppressed. Bystander active suppression may be the main mechanism of oral CII in the suppression of synoviocyte function.
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<meta content="2003-03-01" name="eprints.date" />
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<meta content="Clinical & Experimental Immunology" name="eprints.publication" />
<meta content="132" name="eprints.volume" />
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<meta content="Ding, Changhai and Li, Qin and Xiong, Z.Y. and Zhou, A.W. and Jones, Graeme and Xu, S.Y. (2003) Oral administration of type II collagen suppresses pro-inflammatory mediator production by synoviocytes in rats with adjuvant arthritis. Clinical & Experimental Immunology, 132 (3). pp. 416-423. ISSN 0009-9104" name="eprints.citation" />
<meta content="http://eprints.utas.edu.au/945/1/ClinExpImmunol.pdf" name="eprints.document_url" />
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<meta content="Oral administration of type II collagen suppresses pro-inflammatory mediator production by synoviocytes in rats with adjuvant arthritis
" name="DC.title" />
<meta content="Ding, Changhai" name="DC.creator" />
<meta content="Li, Qin" name="DC.creator" />
<meta content="Xiong, Z.Y." name="DC.creator" />
<meta content="Zhou, A.W." name="DC.creator" />
<meta content="Jones, Graeme" name="DC.creator" />
<meta content="Xu, S.Y." name="DC.creator" />
<meta content="320503 Clinical Pharmacology and Therapeutics" name="DC.subject" />
<meta content="The objective of this study was to investigate the effect of the oral administration of type II collagen (CII) on pro-inflammatory mediator production by synoviocytes in rats with adjuvant arthritis (AA). Sprague-Dawley rats were fed with bovine CII either before immunization with Complete Freund's adjuvant (CFA) or after initiation of arthritis. Hind paw secondary swelling was measured and synoviocytes were harvested. Sera from portal vein of oral tolerized rats were collected and in vitro synoviocytes culture or synoviocytes-Peyer's Patches (PP) cells coculture system were developed. Interleukin (IL)-1 activity was measured by a mouse thymocyte activation assayed by MTT dye reduction and tumour necrosis factor (TNF) activity was measured by an L929 cytotoxicity bioassay. Nitric oxide (NO) and malondialdehyde (MDA) levels were measured by biochemical methods. We found that feeding with CII (5, 50 and 500 ug/kg) for 7 days before immunization significantly suppressed hind paw secondary swelling measured at day 16, 20, 24 and 28 (all P< 0.01) and pro-inflammatory mediator (IL-1, TNF, NO and MDA) production by synoviocytes (all P< 0.01) in rats with AA. Feeding with CII (5, 50 and 500 ug/kg) for 7 days after initiation of arthritis had a similar effect. CII (1, 10, 100 ug/ml) had no effect on IL-1 and TNF production by synoviocytes in vitro, but CII 10 ug/ml suppressed IL-1 and TNF production by synoviocytes-PP cells coculture system (P < 0.01), which was antagonized by anti-TGF-beta antibody (10 ug/ml) (P < 0.01). Portal serum (1 : 10) from oral tolerized rats suppressed IL-1 and TNF production by synoviocytes (P < 0.01), which was also antagonized by anti-TGF-beta antibody (10 ug/ml) (P < 0.01). We conclude that oral administration of CII had prophylactic and therapeutic effects on AA and over-production of IL-1, TNF, NO and MDA by synoviocytes was suppressed. Bystander active suppression may be the main mechanism of oral CII in the suppression of synoviocyte function.
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<h1 class="ep_tm_pagetitle">Oral administration of type II collagen suppresses pro-inflammatory mediator production by synoviocytes in rats with adjuvant arthritis</h1>
<p style="margin-bottom: 1em" class="not_ep_block"><span class="person_name">Ding, Changhai</span> and <span class="person_name">Li, Qin</span> and <span class="person_name">Xiong, Z.Y.</span> and <span class="person_name">Zhou, A.W.</span> and <span class="person_name">Jones, Graeme</span> and <span class="person_name">Xu, S.Y.</span> (2003) <xhtml:em>Oral administration of type II collagen suppresses pro-inflammatory mediator production by synoviocytes in rats with adjuvant arthritis.</xhtml:em> Clinical & Experimental Immunology, 132 (3). pp. 416-423. ISSN 0009-9104</p><p style="margin-bottom: 1em" class="not_ep_block"></p><table style="margin-bottom: 1em" class="not_ep_block"><tr><td valign="top" style="text-align:center"><a href="http://eprints.utas.edu.au/945/1/ClinExpImmunol.pdf"><img alt="[img]" src="http://eprints.utas.edu.au/style/images/fileicons/application_pdf.png" class="ep_doc_icon" border="0" /></a></td><td valign="top"><a href="http://eprints.utas.edu.au/945/1/ClinExpImmunol.pdf"><span class="ep_document_citation">PDF</span></a> - Full text restricted - Requires a PDF viewer<br />114Kb</td></tr><tr><td valign="top" style="text-align:center"><a onmouseover="EPJS_ShowPreview( event, 'doc_preview_985' );" href="http://eprints.utas.edu.au/945/2/Ding_REV.pdf" onmouseout="EPJS_HidePreview( event, 'doc_preview_985' );"><img alt="[img]" src="http://eprints.utas.edu.au/style/images/fileicons/application_pdf.png" class="ep_doc_icon" border="0" /></a><div class="ep_preview" id="doc_preview_985"><table><tr><td><img alt="" src="http://eprints.utas.edu.au/945/thumbnails/2/preview.png" class="ep_preview_image" border="0" /><div class="ep_preview_title">Preview</div></td></tr></table></div></td><td valign="top"><a href="http://eprints.utas.edu.au/945/2/Ding_REV.pdf"><span class="ep_document_citation">PDF (Author Version)</span></a> - Requires a PDF viewer<br />98Kb</td></tr></table><p style="margin-bottom: 1em" class="not_ep_block">Official URL: <a href="http://dx.doi.org/10.1046/j.1365-2249.2003.02167.x">http://dx.doi.org/10.1046/j.1365-2249.2003.02167.x</a></p><div class="not_ep_block"><h2>Abstract</h2><p style="padding-bottom: 16px; text-align: left; margin: 1em auto 0em auto">The objective of this study was to investigate the effect of the oral administration of type II collagen (CII) on pro-inflammatory mediator production by synoviocytes in rats with adjuvant arthritis (AA). Sprague-Dawley rats were fed with bovine CII either before immunization with Complete Freund's adjuvant (CFA) or after initiation of arthritis. Hind paw secondary swelling was measured and synoviocytes were harvested. Sera from portal vein of oral tolerized rats were collected and in vitro synoviocytes culture or synoviocytes-Peyer's Patches (PP) cells coculture system were developed. Interleukin (IL)-1 activity was measured by a mouse thymocyte activation assayed by MTT dye reduction and tumour necrosis factor (TNF) activity was measured by an L929 cytotoxicity bioassay. Nitric oxide (NO) and malondialdehyde (MDA) levels were measured by biochemical methods. We found that feeding with CII (5, 50 and 500 ug/kg) for 7 days before immunization significantly suppressed hind paw secondary swelling measured at day 16, 20, 24 and 28 (all P< 0.01) and pro-inflammatory mediator (IL-1, TNF, NO and MDA) production by synoviocytes (all P< 0.01) in rats with AA. Feeding with CII (5, 50 and 500 ug/kg) for 7 days after initiation of arthritis had a similar effect. CII (1, 10, 100 ug/ml) had no effect on IL-1 and TNF production by synoviocytes in vitro, but CII 10 ug/ml suppressed IL-1 and TNF production by synoviocytes-PP cells coculture system (P < 0.01), which was antagonized by anti-TGF-beta antibody (10 ug/ml) (P < 0.01). Portal serum (1 : 10) from oral tolerized rats suppressed IL-1 and TNF production by synoviocytes (P < 0.01), which was also antagonized by anti-TGF-beta antibody (10 ug/ml) (P < 0.01). We conclude that oral administration of CII had prophylactic and therapeutic effects on AA and over-production of IL-1, TNF, NO and MDA by synoviocytes was suppressed. Bystander active suppression may be the main mechanism of oral CII in the suppression of synoviocyte function.
</p></div><table style="margin-bottom: 1em" cellpadding="3" class="not_ep_block" border="0"><tr><th valign="top" class="ep_row">Item Type:</th><td valign="top" class="ep_row">Article</td></tr><tr><th valign="top" class="ep_row">Additional Information:</th><td valign="top" class="ep_row">The definitive version is available at www.blackwell-synergy.com. Visit - http://www.blackwell-synergy.com/doi/abs/10.1046/j.1365-2249.2003.02167.x</td></tr><tr><th valign="top" class="ep_row">Keywords:</th><td valign="top" class="ep_row">type II collagen, inflammatory mediator, oral tolerance, arthritis </td></tr><tr><th valign="top" class="ep_row">Subjects:</th><td valign="top" class="ep_row"><a href="http://eprints.utas.edu.au/view/subjects/320503.html">320000 Medical and Health Sciences > 320500 Pharmacology and Pharmaceutical Sciences > 320503 Clinical Pharmacology and Therapeutics</a></td></tr><tr><th valign="top" class="ep_row">ID Code:</th><td valign="top" class="ep_row">945</td></tr><tr><th valign="top" class="ep_row">Deposited By:</th><td valign="top" class="ep_row"><span class="ep_name_citation"><span class="person_name">Dr Changhai / C Ding</span></span></td></tr><tr><th valign="top" class="ep_row">Deposited On:</th><td valign="top" class="ep_row">30 Apr 2007</td></tr><tr><th valign="top" class="ep_row">Last Modified:</th><td valign="top" class="ep_row">09 Jan 2008 02:30</td></tr><tr><th valign="top" class="ep_row">ePrint Statistics:</th><td valign="top" class="ep_row"><a target="ePrintStats" href="/es/index.php?action=show_detail_eprint;id=945;">View statistics for this ePrint</a></td></tr></table><p align="right">Repository Staff Only: <a href="http://eprints.utas.edu.au/cgi/users/home?screen=EPrint::View&eprintid=945">item control page</a></p>
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