<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN"
"http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd">
<html>
<head>
<title>UTas ePrints - Temperature effects on the dynamics of gonad and oocyte development in captive wild-caught blacklip (Haliotis rubra) and greenlip (H. laevigata) abalone</title>
<script type="text/javascript" src="http://eprints.utas.edu.au/javascript/auto.js"><!-- padder --></script>
<style type="text/css" media="screen">@import url(http://eprints.utas.edu.au/style/auto.css);</style>
<style type="text/css" media="print">@import url(http://eprints.utas.edu.au/style/print.css);</style>
<link rel="icon" href="/images/eprints/favicon.ico" type="image/x-icon" />
<link rel="shortcut icon" href="/images/eprints/favicon.ico" type="image/x-icon" />
<link rel="Top" href="http://eprints.utas.edu.au/" />
<link rel="Search" href="http://eprints.utas.edu.au/cgi/search" />
<meta content="Grubert, Mark Andrew" name="eprints.creators_name" />
<meta content="Ritar, Arthur J." name="eprints.creators_name" />
<meta content="" name="eprints.creators_id" />
<meta content="Arthur.Ritar@utas.edu.au" name="eprints.creators_id" />
<meta content="article" name="eprints.type" />
<meta content="2007-08-20" name="eprints.datestamp" />
<meta content="2008-01-08 15:30:00" name="eprints.lastmod" />
<meta content="show" name="eprints.metadata_visibility" />
<meta content="Temperature effects on the dynamics of gonad and oocyte development in captive wild-caught blacklip (Haliotis rubra) and greenlip (H. laevigata) abalone" name="eprints.title" />
<meta content="pub" name="eprints.ispublished" />
<meta content="300701" name="eprints.subjects" />
<meta content="300703" name="eprints.subjects" />
<meta content="public" name="eprints.full_text_status" />
<meta content="Biological zero point; Haliotis laevigata; Haliotis rubra; Modified gonad bulk index; Oocyte morphometrics; Temperature; Visual gonad index" name="eprints.keywords" />
<meta content="Wild-caught blacklip (Haliotis rubra) and greenlip (H. laevigata) abalone were held
from spent condition at 12 degrees C, 14 degrees C, 16 degrees C or 18 degrees C and routinely sampled to examine gonad
development. Descriptors of gross structure included the Visual Gonad Index (VGI) and
the Modified Gonad Bulk Index (MGBI). Oocyte Diameter Ratio (ODR) and oocyte
volume (based on an ellipsoid) were used as descriptors of ovarian microstructure. For
each species, the rate of increase in the VGI, MGBI and oocyte volume of animals held at
different temperatures were used to estimate the Biological Zero Point (BZP), the critical
temperature below which no development occurs. BZP estimates derived from the daily
increase in VGI and oocyte volume were similar (7.8 degrees C and 7.6 degrees C for blacklip abalone;
6.9 degrees C and 6.8 degrees C for greenlip abalone, respectively), but those based on the increase in
MGBI were up to 1.8 degrees C lower (6.0 degrees C and 5.7 degrees C, for blacklip and greenlip abalone,
respectively). The mean MGBI, in terms of gonad volume per gram of shucked animal
weight, ranged from 5-68 mm3g-1 and 5-58 mm3g-1 for blacklip and greenlip abalone,
respectively. The ODR indicated that oocyte shape was highly variable in oocytes < 90 um
diameter in both species. Above 90 um, ODR values increased proportionally with oocyte
size, indicating a transition in shape from elliptical to round. Ranges for mean oocyte
volume for blacklip and greenlip abalone were 0.15-1.4 x 106 um3 and 0.02-1.83 x 106
um3, respectively. The pattern of oocyte growth relative to temperature for both species is
illustrated using tables of standardized residuals. Determination of the BZP for blacklip
and greenlip abalone enables the calculation of the Effective Accumulative Temperature
EAT; the cumulative difference between the water temperature and the BZP, calculated
daily) for gamete maturation of these species. This in turn facilitates predictive and
deductive estimates of the completion of this process (when water temperature is known)
in either natural or artificial (i.e. culture) environments." name="eprints.abstract" />
<meta content="2004-11" name="eprints.date" />
<meta content="published" name="eprints.date_type" />
<meta content="Invertebrate Reproduction and Development" name="eprints.publication" />
<meta content="45" name="eprints.volume" />
<meta content="3" name="eprints.number" />
<meta content="185-196" name="eprints.pagerange" />
<meta content="UNSPECIFIED" name="eprints.thesis_type" />
<meta content="TRUE" name="eprints.refereed" />
<meta content="0792-4259" name="eprints.issn" />
<meta content="http://www.isird.org/journal.html" name="eprints.official_url" />
<meta content="Ault, J.S., Some quantitative aspects of reproduction and growth of the red abalone, Haliotis rufescens Swainson. Journal of the World Aquaculture Society, 16 (1985) 398-425.
Capinpin, E.C., Encena, V.C. and Bayona, N.C., Studies on the reproductive biology of the Donkey's ear abalone, Haliotis asinina Linne. Aquaculture, 166 (1998) 141-150.
Clark, F.N., Maturity of the Californian sardine (Sardina caerulea), determined by ova diameter measurements. Div. Fish Game Calif., Fish Bull., 42 (1934) 1-49.
Grant, A. and Tyler, P.A., The analysis of data in studies of invertebrate reproduction. I. Introduction and statistical analysis of gonad indices and maturity indices. Int. J. Invertebr. Reprod., 6 (1983a) 259-269.
Grant, A. and Tyler, P.A., The analysis of data in studies of invertebrate reproduction. II. The analysis of oocyte size/frequency data, and comparison of different types of data. Int. J. Invertebr. Reprod., 6 (1983b) 271-283.
Grubert, M.A. and Ritar, A.J., Abalone broodstock conditioning system at TAFI MRL. Austasia Aquaculture, 16 (2002) 29-36
Hahn, K.O., Gonad reproductive cycles. In: Hahn, K.O. (ed.), Handbook of Culture of Abalone and Other Marine Gastropods, CRC Press, Boca Raton, 1989, pp. 13-39.
Hahn, K.O., Gametogenic cycle of the Japanese abalone (ezoawabi), Haliotis discus hannai, during conditioning with effective accumulative temperature. Aquaculture, 122 (1994) 227-236.
Harrison, A.J. and Grant, J.F., Progress in abalone research. Tasmanian Fish. Res., 5 (1971) 1-10.
Jebreen, E.J., Counihan, R.T., Fielder, D.R. and Degnan, B.M., Synchronous oogenesis during the semilunar spawning cycle of the tropical abalone Haliotis asinina. J. Shellfish Res., 19 (2000) 845-851.
Kabir, N.M.J., Environmental, chemical and hormonal regulation of reproduction in two commercially important New Zealand abalone, Haliotis iris and H. australis. PhD dissertation, Dunedin, University of Otago, 2001, 236 pp.
Kikuchi, S. and Uki, N., Technical study of artificial spawning of abalone, genus Haliotis I. Relationship between water temperature and advancing sexual maturity of Haliotis discus hannai Ino. Bull. Tohoku Reg. Fish. Res. Lab., 33 (1974a) 69-78 (in Japanese with English abstract).
Kikuchi, S. and Uki, N., Technical study of artificial spawning of abalone, genus Haliotis V. Relationship between water temperature and advancing sexual maturity of Haliotis discus Reeve. Bull. Tohoku Reg. Fish. Res. Lab., 34 (1974b) 77-85 (in Japanese with English abstract).
Lleonart, M., A gonad conditioning study of the greenlip abalone Haliotis laevigata. MS thesis, Launceston, University of Tasmania, 1992, 162 pp.
McShane, P.E., Beinssen, K.H.H., Smith, M.G., O'Conner, S. and Hickman, N.J., Reproductive biology of blacklip abalone Haliotis ruber Leach from four Victorian populations. Department of Conservation Forests & Lands, Fisheries and Wildlife Service, Technical Report No. 55, 1986, 13 pp.
Moss, G.A., Effect of temperature on the breeding cycle and spawning success of the New Zealand abalone, Haliotis australis. N. Z. J. Mar. Freshwat. Res., 32 (1998) 139-146.
Newman, G.C., Reproduction of the South African abalone Haliotis midae. Investl Rep. Div. Sea Fish. S. Afr., 64 (1967) 1-24.
Quinn, G.P. and Keogh, M.J., Experimental design and data analysis for biologists. Cambridge University Press, Cambridge, 2002, 537 pp.
Sawatpeera, S., Upatham, E.S., Kruatrachue, M., Chitramvong, Y.P., Songchaeng, P., Pumthong, T. and Nugranad, J., Larval development in Haliotis asinina Linnaeus. J. Shellfish Res., 20 (2001) 593-601.
Shepherd, S.A. and Laws, H.M., Studies on Southern Australian abalone (Genus Haliotis) II. Reproduction of five species. Aust. J. Mar. Freshwat. Res., 24 (1974) 49-62.
Thompson, W.F., A preliminary report on the life-history of the halibut. British Columbia. Comm. Fish. Rept. for 1914, (1915) 76-99.
Tutschulte, T. and Connell, J.H., Reproductive biology of three species of abalones (Haliotis) in Southern California. Veliger, 23 (1981) 195-206.
Wells, F.E. and Mulvay, P., Reproduction and growth of the greenlip abalone Haliotis laevigata on the south coast of Western Australia. Western Australian Department of Fisheries, Perth, 1992, 117 pp.
Wilson, N.H.F. and Schiel, D.R., Reproduction in two species of abalone (Haliotis iris and H. australis) in southern New Zealand. Mar. Freshwater Res., 46 (1995) 629-637.
Wood, A.D. and Buxton, C.D., Aspects of the biology of the abalone Haliotis midae (Linne, 1758) on the east coast of South Africa. 2. Reproduction. S. Afr. J. Mar. Sci., 17 (1996) 69-78.
Zar, J.D., Biostatistical analysis, 3rd Edition. Prentice Hall, New York, 1996, 659 pp." name="eprints.referencetext" />
<meta content="Grubert, Mark Andrew and Ritar, Arthur J. (2004) Temperature effects on the dynamics of gonad and oocyte development in captive wild-caught blacklip (Haliotis rubra) and greenlip (H. laevigata) abalone. Invertebrate Reproduction and Development, 45 (3). pp. 185-196. ISSN 0792-4259" name="eprints.citation" />
<meta content="http://eprints.utas.edu.au/1530/1/ritar-IRD-author.pdf" name="eprints.document_url" />
<link rel="schema.DC" href="http://purl.org/DC/elements/1.0/" />
<meta content="Temperature effects on the dynamics of gonad and oocyte development in captive wild-caught blacklip (Haliotis rubra) and greenlip (H. laevigata) abalone" name="DC.title" />
<meta content="Grubert, Mark Andrew" name="DC.creator" />
<meta content="Ritar, Arthur J." name="DC.creator" />
<meta content="300701 Physiology and Genetics" name="DC.subject" />
<meta content="300703 Aquaculture" name="DC.subject" />
<meta content="Wild-caught blacklip (Haliotis rubra) and greenlip (H. laevigata) abalone were held
from spent condition at 12 degrees C, 14 degrees C, 16 degrees C or 18 degrees C and routinely sampled to examine gonad
development. Descriptors of gross structure included the Visual Gonad Index (VGI) and
the Modified Gonad Bulk Index (MGBI). Oocyte Diameter Ratio (ODR) and oocyte
volume (based on an ellipsoid) were used as descriptors of ovarian microstructure. For
each species, the rate of increase in the VGI, MGBI and oocyte volume of animals held at
different temperatures were used to estimate the Biological Zero Point (BZP), the critical
temperature below which no development occurs. BZP estimates derived from the daily
increase in VGI and oocyte volume were similar (7.8 degrees C and 7.6 degrees C for blacklip abalone;
6.9 degrees C and 6.8 degrees C for greenlip abalone, respectively), but those based on the increase in
MGBI were up to 1.8 degrees C lower (6.0 degrees C and 5.7 degrees C, for blacklip and greenlip abalone,
respectively). The mean MGBI, in terms of gonad volume per gram of shucked animal
weight, ranged from 5-68 mm3g-1 and 5-58 mm3g-1 for blacklip and greenlip abalone,
respectively. The ODR indicated that oocyte shape was highly variable in oocytes < 90 um
diameter in both species. Above 90 um, ODR values increased proportionally with oocyte
size, indicating a transition in shape from elliptical to round. Ranges for mean oocyte
volume for blacklip and greenlip abalone were 0.15-1.4 x 106 um3 and 0.02-1.83 x 106
um3, respectively. The pattern of oocyte growth relative to temperature for both species is
illustrated using tables of standardized residuals. Determination of the BZP for blacklip
and greenlip abalone enables the calculation of the Effective Accumulative Temperature
EAT; the cumulative difference between the water temperature and the BZP, calculated
daily) for gamete maturation of these species. This in turn facilitates predictive and
deductive estimates of the completion of this process (when water temperature is known)
in either natural or artificial (i.e. culture) environments." name="DC.description" />
<meta content="2004-11" name="DC.date" />
<meta content="Article" name="DC.type" />
<meta content="PeerReviewed" name="DC.type" />
<meta content="application/pdf" name="DC.format" />
<meta content="http://eprints.utas.edu.au/1530/1/ritar-IRD-author.pdf" name="DC.identifier" />
<meta content="http://www.isird.org/journal.html" name="DC.relation" />
<meta content="Grubert, Mark Andrew and Ritar, Arthur J. (2004) Temperature effects on the dynamics of gonad and oocyte development in captive wild-caught blacklip (Haliotis rubra) and greenlip (H. laevigata) abalone. Invertebrate Reproduction and Development, 45 (3). pp. 185-196. ISSN 0792-4259" name="DC.identifier" />
<meta content="http://eprints.utas.edu.au/1530/" name="DC.relation" />
<link rel="alternate" href="http://eprints.utas.edu.au/cgi/export/1530/BibTeX/epprod-eprint-1530.bib" title="BibTeX" type="text/plain" />
<link rel="alternate" href="http://eprints.utas.edu.au/cgi/export/1530/ContextObject/epprod-eprint-1530.xml" title="OpenURL ContextObject" type="text/xml" />
<link rel="alternate" href="http://eprints.utas.edu.au/cgi/export/1530/ContextObject::Dissertation/epprod-eprint-1530.xml" title="OpenURL Dissertation" type="text/xml" />
<link rel="alternate" href="http://eprints.utas.edu.au/cgi/export/1530/ContextObject::Journal/epprod-eprint-1530.xml" title="OpenURL Journal" type="text/xml" />
<link rel="alternate" href="http://eprints.utas.edu.au/cgi/export/1530/DC/epprod-eprint-1530.txt" title="Dublin Core" type="text/plain" />
<link rel="alternate" href="http://eprints.utas.edu.au/cgi/export/1530/DIDL/epprod-eprint-1530.xml" title="DIDL" type="text/xml" />
<link rel="alternate" href="http://eprints.utas.edu.au/cgi/export/1530/EndNote/epprod-eprint-1530.enw" title="EndNote" type="text/plain" />
<link rel="alternate" href="http://eprints.utas.edu.au/cgi/export/1530/HTML/epprod-eprint-1530.html" title="HTML Citation" type="text/html; charset=utf-8" />
<link rel="alternate" href="http://eprints.utas.edu.au/cgi/export/1530/METS/epprod-eprint-1530.xml" title="METS" type="text/xml" />
<link rel="alternate" href="http://eprints.utas.edu.au/cgi/export/1530/MODS/epprod-eprint-1530.xml" title="MODS" type="text/xml" />
<link rel="alternate" href="http://eprints.utas.edu.au/cgi/export/1530/RIS/epprod-eprint-1530.ris" title="Reference Manager" type="text/plain" />
<link rel="alternate" href="http://eprints.utas.edu.au/cgi/export/1530/Refer/epprod-eprint-1530.refer" title="Refer" type="text/plain" />
<link rel="alternate" href="http://eprints.utas.edu.au/cgi/export/1530/Simple/epprod-eprint-1530text" title="Simple Metadata" type="text/plain" />
<link rel="alternate" href="http://eprints.utas.edu.au/cgi/export/1530/Text/epprod-eprint-1530.txt" title="ASCII Citation" type="text/plain; charset=utf-8" />
<link rel="alternate" href="http://eprints.utas.edu.au/cgi/export/1530/XML/epprod-eprint-1530.xml" title="EP3 XML" type="text/xml" />
</head>
<body bgcolor="#ffffff" text="#000000" onLoad="loadRoutine(); MM_preloadImages('images/eprints/ePrints_banner_r5_c5_f2.gif','images/eprints/ePrints_banner_r5_c7_f2.gif','images/eprints/ePrints_banner_r5_c8_f2.gif','images/eprints/ePrints_banner_r5_c9_f2.gif','images/eprints/ePrints_banner_r5_c10_f2.gif','images/eprints/ePrints_banner_r5_c11_f2.gif','images/eprints/ePrints_banner_r6_c4_f2.gif')">
<div class="ep_noprint"><noscript><style type="text/css">@import url(http://eprints.utas.edu.au/style/nojs.css);</style></noscript></div>
<table width="795" border="0" cellspacing="0" cellpadding="0">
<tr>
<td><script language="JavaScript1.2">mmLoadMenus();</script>
<table border="0" cellpadding="0" cellspacing="0" width="795">
<!-- fwtable fwsrc="eprints_banner_final2.png" fwbase="ePrints_banner.gif" fwstyle="Dreamweaver" fwdocid = "1249563342" fwnested="0" -->
<tr>
<td><img src="/images/eprints/spacer.gif" width="32" height="1" border="0" alt="" /></td>
<td><img src="/images/eprints/spacer.gif" width="104" height="1" border="0" alt="" /></td>
<td><img src="/images/eprints/spacer.gif" width="44" height="1" border="0" alt="" /></td>
<td><img src="/images/eprints/spacer.gif" width="105" height="1" border="0" alt="" /></td>
<td><img src="/images/eprints/spacer.gif" width="41" height="1" border="0" alt="" /></td>
<td><img src="/images/eprints/spacer.gif" width="16" height="1" border="0" alt="" /></td>
<td><img src="/images/eprints/spacer.gif" width="68" height="1" border="0" alt="" /></td>
<td><img src="/images/eprints/spacer.gif" width="68" height="1" border="0" alt="" /></td>
<td><img src="/images/eprints/spacer.gif" width="68" height="1" border="0" alt="" /></td>
<td><img src="/images/eprints/spacer.gif" width="82" height="1" border="0" alt="" /></td>
<td><img src="/images/eprints/spacer.gif" width="69" height="1" border="0" alt="" /></td>
<td><img src="/images/eprints/spacer.gif" width="98" height="1" border="0" alt="" /></td>
<td><img src="/images/eprints/spacer.gif" width="1" height="1" border="0" alt="" /></td>
</tr>
<tr>
<td colspan="12"><img name="ePrints_banner_r1_c1" src="/images/eprints/ePrints_banner_r1_c1.gif" width="795" height="10" border="0" alt="" /></td>
<td><img src="/images/eprints/spacer.gif" width="1" height="10" border="0" alt="" /></td>
</tr>
<tr>
<td rowspan="6"><img name="ePrints_banner_r2_c1" src="/images/eprints/ePrints_banner_r2_c1.gif" width="32" height="118" border="0" alt="" /></td>
<td rowspan="5"><a href="http://www.utas.edu.au/"><img name="ePrints_banner_r2_c2" src="/images/eprints/ePrints_banner_r2_c2.gif" width="104" height="103" border="0" alt="" /></a></td>
<td colspan="10"><img name="ePrints_banner_r2_c3" src="/images/eprints/ePrints_banner_r2_c3.gif" width="659" height="41" border="0" alt="" /></td>
<td><img src="/images/eprints/spacer.gif" width="1" height="41" border="0" alt="" /></td>
</tr>
<tr>
<td colspan="3"><a href="http://eprints.utas.edu.au/"><img name="ePrints_banner_r3_c3" src="/images/eprints/ePrints_banner_r3_c3.gif" width="190" height="31" border="0" alt="" /></a></td>
<td rowspan="2" colspan="7"><img name="ePrints_banner_r3_c6" src="/images/eprints/ePrints_banner_r3_c6.gif" width="469" height="37" border="0" alt="" /></td>
<td><img src="/images/eprints/spacer.gif" width="1" height="31" border="0" alt="" /></td>
</tr>
<tr>
<td colspan="3"><img name="ePrints_banner_r4_c3" src="/images/eprints/ePrints_banner_r4_c3.gif" width="190" height="6" border="0" alt="" /></td>
<td><img src="/images/eprints/spacer.gif" width="1" height="6" border="0" alt="" /></td>
</tr>
<tr>
<td colspan="2"><img name="ePrints_banner_r5_c3" src="/images/eprints/ePrints_banner_r5_c3.gif" width="149" height="1" border="0" alt="" /></td>
<td rowspan="2" colspan="2"><a href="/information.html" onMouseOut="MM_swapImgRestore();MM_startTimeout()" onMouseOver="MM_showMenu(window.mm_menu_0821132634_0,0,25,null,'ePrints_banner_r5_c5');MM_swapImage('ePrints_banner_r5_c5','','/images/eprints/ePrints_banner_r5_c5_f2.gif',1);"><img name="ePrints_banner_r5_c5" src="/images/eprints/ePrints_banner_r5_c5.gif" width="57" height="25" border="0" alt="About" /></a></td>
<td rowspan="2"><a href="/view/" onMouseOut="MM_swapImgRestore();MM_startTimeout()" onMouseOver="MM_showMenu(window.mm_menu_0821133021_1,0,25,null,'ePrints_banner_r5_c7');MM_swapImage('ePrints_banner_r5_c7','','/images/eprints/ePrints_banner_r5_c7_f2.gif',1);"><img name="ePrints_banner_r5_c7" src="/images/eprints/ePrints_banner_r5_c7.gif" width="68" height="25" border="0" alt="Browse" /></a></td>
<td rowspan="2"><a href="/perl/search/simple" onMouseOut="MM_swapImgRestore();MM_startTimeout()" onMouseOver="MM_showMenu(window.mm_menu_0821133201_2,0,25,null,'ePrints_banner_r5_c8');MM_swapImage('ePrints_banner_r5_c8','','/images/eprints/ePrints_banner_r5_c8_f2.gif',1);"><img name="ePrints_banner_r5_c8" src="/images/eprints/ePrints_banner_r5_c8.gif" width="68" height="25" border="0" alt="Search" /></a></td>
<td rowspan="2"><a href="/perl/register" onMouseOut="MM_swapImgRestore();MM_startTimeout();" onMouseOver="MM_showMenu(window.mm_menu_1018171924_3,0,25,null,'ePrints_banner_r5_c9');MM_swapImage('ePrints_banner_r5_c9','','/images/eprints/ePrints_banner_r5_c9_f2.gif',1);"><img name="ePrints_banner_r5_c9" src="/images/eprints/ePrints_banner_r5_c9.gif" width="68" height="25" border="0" alt="register" /></a></td>
<td rowspan="2"><a href="/perl/users/home" onMouseOut="MM_swapImgRestore();MM_startTimeout()" onMouseOver="MM_showMenu(window.mm_menu_0821133422_4,0,25,null,'ePrints_banner_r5_c10');MM_swapImage('ePrints_banner_r5_c10','','/images/eprints/ePrints_banner_r5_c10_f2.gif',1);"><img name="ePrints_banner_r5_c10" src="/images/eprints/ePrints_banner_r5_c10.gif" width="82" height="25" border="0" alt="user area" /></a></td>
<td rowspan="2"><a href="/help/" onMouseOut="MM_swapImgRestore();MM_startTimeout()" onMouseOver="MM_showMenu(window.mm_menu_0821133514_5,0,25,null,'ePrints_banner_r5_c11');MM_swapImage('ePrints_banner_r5_c11','','/images/eprints/ePrints_banner_r5_c11_f2.gif',1);"><img name="ePrints_banner_r5_c11" src="/images/eprints/ePrints_banner_r5_c11.gif" width="69" height="25" border="0" alt="Help" /></a></td>
<td rowspan="3" colspan="4"><img name="ePrints_banner_r5_c12" src="/images/eprints/ePrints_banner_r5_c12.gif" width="98" height="40" border="0" alt="" /></td>
<td><img src="/images/eprints/spacer.gif" width="1" height="1" border="0" alt="" /></td>
</tr>
<tr>
<td rowspan="2"><img name="ePrints_banner_r6_c3" src="/images/eprints/ePrints_banner_r6_c3.gif" width="44" height="39" border="0" alt="ePrints home" /></td>
<td><a href="/" onMouseOut="MM_swapImgRestore()" onMouseOver="MM_swapImage('ePrints_banner_r6_c4','','/images/eprints/ePrints_banner_r6_c4_f2.gif',1);"><img name="ePrints_banner_r6_c4" src="/images/eprints/ePrints_banner_r6_c4.gif" width="105" height="24" border="0" alt="ePrints home" /></a></td>
<td><img src="/images/eprints/spacer.gif" width="1" height="24" border="0" alt="" /></td>
</tr>
<tr>
<td><img name="ePrints_banner_r7_c2" src="/images/eprints/ePrints_banner_r7_c2.gif" width="104" height="15" border="0" alt="" /></td>
<td colspan="8"><img name="ePrints_banner_r7_c4" src="/images/eprints/ePrints_banner_r7_c4.gif" width="517" height="15" border="0" alt="" /></td>
<td><img src="/images/eprints/spacer.gif" width="1" height="15" border="0" alt="" /></td>
</tr>
</table></td>
</tr>
<tr><td><table width="100%" style="font-size: 90%; border: solid 1px #ccc; padding: 3px"><tr>
<td align="left"><a href="http://eprints.utas.edu.au/cgi/users/home">Login</a> | <a href="http://eprints.utas.edu.au/cgi/register">Create Account</a></td>
<td align="right" style="white-space: nowrap">
<form method="get" accept-charset="utf-8" action="http://eprints.utas.edu.au/cgi/search" style="display:inline">
<input class="ep_tm_searchbarbox" size="20" type="text" name="q" />
<input class="ep_tm_searchbarbutton" value="Search" type="submit" name="_action_search" />
<input type="hidden" name="_order" value="bytitle" />
<input type="hidden" name="basic_srchtype" value="ALL" />
<input type="hidden" name="_satisfyall" value="ALL" />
</form>
</td>
</tr></table></td></tr>
<tr>
<td class="toplinks"><!-- InstanceBeginEditable name="content" -->
<div align="center">
<table width="720" class="ep_tm_main"><tr><td align="left">
<h1 class="ep_tm_pagetitle">Temperature effects on the dynamics of gonad and oocyte development in captive wild-caught blacklip (Haliotis rubra) and greenlip (H. laevigata) abalone</h1>
<p style="margin-bottom: 1em" class="not_ep_block"><span class="person_name">Grubert, Mark Andrew</span> and <span class="person_name">Ritar, Arthur J.</span> (2004) <xhtml:em>Temperature effects on the dynamics of gonad and oocyte development in captive wild-caught blacklip (Haliotis rubra) and greenlip (H. laevigata) abalone.</xhtml:em> Invertebrate Reproduction and Development, 45 (3). pp. 185-196. ISSN 0792-4259</p><p style="margin-bottom: 1em" class="not_ep_block"></p><table style="margin-bottom: 1em" class="not_ep_block"><tr><td valign="top" style="text-align:center"><a onmouseover="EPJS_ShowPreview( event, 'doc_preview_1967' );" href="http://eprints.utas.edu.au/1530/1/ritar-IRD-author.pdf" onmouseout="EPJS_HidePreview( event, 'doc_preview_1967' );"><img alt="[img]" src="http://eprints.utas.edu.au/style/images/fileicons/application_pdf.png" class="ep_doc_icon" border="0" /></a><div class="ep_preview" id="doc_preview_1967"><table><tr><td><img alt="" src="http://eprints.utas.edu.au/1530/thumbnails/1/preview.png" class="ep_preview_image" border="0" /><div class="ep_preview_title">Preview</div></td></tr></table></div></td><td valign="top"><a href="http://eprints.utas.edu.au/1530/1/ritar-IRD-author.pdf"><span class="ep_document_citation">PDF (Author Version)</span></a> - Requires a PDF viewer<br />269Kb</td></tr></table><p style="margin-bottom: 1em" class="not_ep_block">Official URL: <a href="http://www.isird.org/journal.html">http://www.isird.org/journal.html</a></p><div class="not_ep_block"><h2>Abstract</h2><p style="padding-bottom: 16px; text-align: left; margin: 1em auto 0em auto">Wild-caught blacklip (Haliotis rubra) and greenlip (H. laevigata) abalone were held
from spent condition at 12 degrees C, 14 degrees C, 16 degrees C or 18 degrees C and routinely sampled to examine gonad
development. Descriptors of gross structure included the Visual Gonad Index (VGI) and
the Modified Gonad Bulk Index (MGBI). Oocyte Diameter Ratio (ODR) and oocyte
volume (based on an ellipsoid) were used as descriptors of ovarian microstructure. For
each species, the rate of increase in the VGI, MGBI and oocyte volume of animals held at
different temperatures were used to estimate the Biological Zero Point (BZP), the critical
temperature below which no development occurs. BZP estimates derived from the daily
increase in VGI and oocyte volume were similar (7.8 degrees C and 7.6 degrees C for blacklip abalone;
6.9 degrees C and 6.8 degrees C for greenlip abalone, respectively), but those based on the increase in
MGBI were up to 1.8 degrees C lower (6.0 degrees C and 5.7 degrees C, for blacklip and greenlip abalone,
respectively). The mean MGBI, in terms of gonad volume per gram of shucked animal
weight, ranged from 5-68 mm3g-1 and 5-58 mm3g-1 for blacklip and greenlip abalone,
respectively. The ODR indicated that oocyte shape was highly variable in oocytes < 90 um
diameter in both species. Above 90 um, ODR values increased proportionally with oocyte
size, indicating a transition in shape from elliptical to round. Ranges for mean oocyte
volume for blacklip and greenlip abalone were 0.15-1.4 x 106 um3 and 0.02-1.83 x 106
um3, respectively. The pattern of oocyte growth relative to temperature for both species is
illustrated using tables of standardized residuals. Determination of the BZP for blacklip
and greenlip abalone enables the calculation of the Effective Accumulative Temperature
EAT; the cumulative difference between the water temperature and the BZP, calculated
daily) for gamete maturation of these species. This in turn facilitates predictive and
deductive estimates of the completion of this process (when water temperature is known)
in either natural or artificial (i.e. culture) environments.</p></div><table style="margin-bottom: 1em" cellpadding="3" class="not_ep_block" border="0"><tr><th valign="top" class="ep_row">Item Type:</th><td valign="top" class="ep_row">Article</td></tr><tr><th valign="top" class="ep_row">Keywords:</th><td valign="top" class="ep_row">Biological zero point; Haliotis laevigata; Haliotis rubra; Modified gonad bulk index; Oocyte morphometrics; Temperature; Visual gonad index</td></tr><tr><th valign="top" class="ep_row">Subjects:</th><td valign="top" class="ep_row"><a href="http://eprints.utas.edu.au/view/subjects/300701.html">300000 Agricultural, Veterinary and Environmental Sciences > 300700 Fisheries Sciences > 300701 Physiology and Genetics</a><br /><a href="http://eprints.utas.edu.au/view/subjects/300703.html">300000 Agricultural, Veterinary and Environmental Sciences > 300700 Fisheries Sciences > 300703 Aquaculture</a></td></tr><tr><th valign="top" class="ep_row">ID Code:</th><td valign="top" class="ep_row">1530</td></tr><tr><th valign="top" class="ep_row">Deposited By:</th><td valign="top" class="ep_row"><span class="ep_name_citation"><span class="person_name">Dr Arthur J Ritar</span></span></td></tr><tr><th valign="top" class="ep_row">Deposited On:</th><td valign="top" class="ep_row">20 Aug 2007</td></tr><tr><th valign="top" class="ep_row">Last Modified:</th><td valign="top" class="ep_row">09 Jan 2008 02:30</td></tr><tr><th valign="top" class="ep_row">ePrint Statistics:</th><td valign="top" class="ep_row"><a target="ePrintStats" href="/es/index.php?action=show_detail_eprint;id=1530;">View statistics for this ePrint</a></td></tr></table><p align="right">Repository Staff Only: <a href="http://eprints.utas.edu.au/cgi/users/home?screen=EPrint::View&eprintid=1530">item control page</a></p>
</td></tr></table>
</div>
<!-- InstanceEndEditable --></td>
</tr>
<tr>
<td><!-- #BeginLibraryItem "/Library/footer_eprints.lbi" -->
<table width="795" border="0" align="left" cellpadding="0" class="footer">
<tr valign="top">
<td colspan="2"><div align="center"><a href="http://www.utas.edu.au">UTAS home</a> | <a href="http://www.utas.edu.au/library/">Library home</a> | <a href="/">ePrints home</a> | <a href="/contact.html">contact</a> | <a href="/information.html">about</a> | <a href="/view/">browse</a> | <a href="/perl/search/simple">search</a> | <a href="/perl/register">register</a> | <a href="/perl/users/home">user area</a> | <a href="/help/">help</a></div><br /></td>
</tr>
<tr><td colspan="2"><p><img src="/images/eprints/footerline.gif" width="100%" height="4" /></p></td></tr>
<tr valign="top">
<td width="68%" class="footer">Authorised by the University Librarian<br />
© University of Tasmania ABN 30 764 374 782<br />
<a href="http://www.utas.edu.au/cricos/">CRICOS Provider Code 00586B</a> | <a href="http://www.utas.edu.au/copyright/copyright_disclaimers.html">Copyright & Disclaimers</a> | <a href="http://www.utas.edu.au/accessibility/index.html">Accessibility</a> | <a href="http://eprints.utas.edu.au/feedback/">Site Feedback</a> </td>
<td width="32%"><div align="right">
<p align="right" class="NoPrint"><a href="http://www.utas.edu.au/"><img src="http://www.utas.edu.au/shared/logos/unioftasstrip.gif" alt="University of Tasmania Home Page" width="260" height="16" border="0" align="right" /></a></p>
<p align="right" class="NoPrint"><a href="http://www.utas.edu.au/"><br />
</a></p>
</div></td>
</tr>
<tr valign="top">
<td><p> </p></td>
<td><div align="right"><span class="NoPrint"><a href="http://www.eprints.org/software/"><img src="/images/eprintslogo.gif" alt="ePrints logo" width="77" height="29" border="0" align="bottom" /></a></span></div></td>
</tr>
</table>
<!-- #EndLibraryItem -->
<div align="center"></div></td>
</tr>
</table>
</body>
</html>
