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<title>UTas ePrints - Biochemical composition during growth and starvation of early larval stages of cultured spiny lobster (Jasus edwardsii) phyllosoma</title>
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<meta content="Ritar, Arthur J." name="eprints.creators_name" />
<meta content="Dunstan, Graeme A." name="eprints.creators_name" />
<meta content="Crear, Bradley J." name="eprints.creators_name" />
<meta content="Brown, Malcolm A." name="eprints.creators_name" />
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<meta content="2007-08-09" name="eprints.datestamp" />
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<meta content="Biochemical composition during growth and starvation of early larval stages of cultured spiny lobster (Jasus edwardsii) phyllosoma" name="eprints.title" />
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<meta content="Artemia; Ascorbic acid; Fatty acid; Jasus edwardsii; Nutrition; Phyllosoma; Spiny lobster; Starvation" name="eprints.keywords" />
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<meta content="We examined biochemical changes accompanying feeding and starvation from hatch to Stage VI (day 74 after hatch) in spiny lobster, Jasus edwardsii, phyllosoma larvae. Larval dry weights (dw) increased 17-fold from hatch (80 +/- 1 ug) to Stage VI (1415 +/- 44 ug). Larvae starved for 6-11 days at Stages II, IV and VI were 14-40% lighter than their fed counterparts fed enriched Artemia. The increases and losses in total dw during feeding and starvation were associated with changes in the content of protein (constituting 31.4-41.7% of dw) and carbohydrate (constituting 2.6-5.3% of dw), while larger changes in lipid content indicated its greater importance as an energy substrate. Lipid content increased from 7.9% of dw at hatch to its highest of 12.5% at Stage IV, but declined by 50% or more during starvation. This suggests that protein, carbohydrate and lipid are all important energy stores, although lipids are catabolised at a greater rate during food deprivation. The principal lipid class was polar lipid (79-92% of total lipid), followed by sterol (6-20%), with triacylglycerol and other lipid classes at <2%. Polar lipids were catabolized and sterols were conserved during starvation. Changes in the fatty acid profile had mostly occurred before the first moult at day 8 after hatch, with gradual changes thereafter to Stage VI, reflecting their abundance in the Artemia diet. There was some conservation of the major essential fatty acids (EFA), 20:4n-6, 20:5n-3, 22:6n-3, and the fatty acid profile showed large gains in the C18 PUFA, 18:1n-9, 18:2n-6. Ascorbic acid content increased 10-fold from hatch to the end of Stage I (36 and 333 ug g-1 dw, respectively), while the content at the end of Stage II was higher in fed than starved larvae (439 and 174 ug g-1 dw, respectively). Our study will assist in the development of alternatives to nutritionally incomplete diets, such as live ongrown Artemia, to meet the requirements of phyllosoma in culture." name="eprints.abstract" />
<meta content="2003-10" name="eprints.date" />
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<meta content="Comparative Biochemistry and Physiology Part A" name="eprints.publication" />
<meta content="136" name="eprints.volume" />
<meta content="2" name="eprints.number" />
<meta content="353-370" name="eprints.pagerange" />
<meta content="10.1016/S1095-6433(03)00167-3" name="eprints.id_number" />
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<meta content="Abrunhosa, F.A., Kittaka, J. 1997. Effect of starvation on the first larvae of Homarus americanus (Decapoda, Nephropidae) and phyllosomas of Jasus verreauxi and J. edwardsii (Decapoda Palinuridae). Bulletin of Marine Science 61, 73-80.
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Brown, M., Dunstan, G., Augerinos, M. 2001. Biochemistry of wild and cultured phyllosoma. In: FRDC Final Report. Rock Lobster Enhancement and Aquaculture Subprogram: The Development of Rock Lobster Propagation Techniques for Aquaculture in Australia. (Eds. B.J. Crear and P.R. Hart) (FRDC Project No. 1999/315; ISSN 1441-8487). pp. 33-37.
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Tong, L. J., Moss, G.A., Paewai, M., Pickering, T.D. 1997. Effect of brine shrimp numbers on the growth and survival of early stage phyllosoma larvae of the rock lobster Jasus edwardsii. Marine and Freshwater Research 48, 935-940." name="eprints.referencetext" />
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<meta content="We examined biochemical changes accompanying feeding and starvation from hatch to Stage VI (day 74 after hatch) in spiny lobster, Jasus edwardsii, phyllosoma larvae. Larval dry weights (dw) increased 17-fold from hatch (80 +/- 1 ug) to Stage VI (1415 +/- 44 ug). Larvae starved for 6-11 days at Stages II, IV and VI were 14-40% lighter than their fed counterparts fed enriched Artemia. The increases and losses in total dw during feeding and starvation were associated with changes in the content of protein (constituting 31.4-41.7% of dw) and carbohydrate (constituting 2.6-5.3% of dw), while larger changes in lipid content indicated its greater importance as an energy substrate. Lipid content increased from 7.9% of dw at hatch to its highest of 12.5% at Stage IV, but declined by 50% or more during starvation. This suggests that protein, carbohydrate and lipid are all important energy stores, although lipids are catabolised at a greater rate during food deprivation. The principal lipid class was polar lipid (79-92% of total lipid), followed by sterol (6-20%), with triacylglycerol and other lipid classes at <2%. Polar lipids were catabolized and sterols were conserved during starvation. Changes in the fatty acid profile had mostly occurred before the first moult at day 8 after hatch, with gradual changes thereafter to Stage VI, reflecting their abundance in the Artemia diet. There was some conservation of the major essential fatty acids (EFA), 20:4n-6, 20:5n-3, 22:6n-3, and the fatty acid profile showed large gains in the C18 PUFA, 18:1n-9, 18:2n-6. Ascorbic acid content increased 10-fold from hatch to the end of Stage I (36 and 333 ug g-1 dw, respectively), while the content at the end of Stage II was higher in fed than starved larvae (439 and 174 ug g-1 dw, respectively). Our study will assist in the development of alternatives to nutritionally incomplete diets, such as live ongrown Artemia, to meet the requirements of phyllosoma in culture." name="DC.description" />
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<h1 class="ep_tm_pagetitle">Biochemical composition during growth and starvation of early larval stages of cultured spiny lobster (Jasus edwardsii) phyllosoma</h1>
<p style="margin-bottom: 1em" class="not_ep_block"><span class="person_name">Ritar, Arthur J.</span> and <span class="person_name">Dunstan, Graeme A.</span> and <span class="person_name">Crear, Bradley J.</span> and <span class="person_name">Brown, Malcolm A.</span> (2003) <xhtml:em>Biochemical composition during growth and starvation of early larval stages of cultured spiny lobster (Jasus edwardsii) phyllosoma.</xhtml:em> Comparative Biochemistry and Physiology Part A, 136 (2). pp. 353-370.</p><p style="margin-bottom: 1em" class="not_ep_block"></p><table style="margin-bottom: 1em" class="not_ep_block"><tr><td valign="top" style="text-align:center"><a onmouseover="EPJS_ShowPreview( event, 'doc_preview_2021' );" href="http://eprints.utas.edu.au/1559/1/ritar-comp-bio.pdf" onmouseout="EPJS_HidePreview( event, 'doc_preview_2021' );"><img alt="[img]" src="http://eprints.utas.edu.au/style/images/fileicons/application_pdf.png" class="ep_doc_icon" border="0" /></a><div class="ep_preview" id="doc_preview_2021"><table><tr><td><img alt="" src="http://eprints.utas.edu.au/1559/thumbnails/1/preview.png" class="ep_preview_image" border="0" /><div class="ep_preview_title">Preview</div></td></tr></table></div></td><td valign="top"><a href="http://eprints.utas.edu.au/1559/1/ritar-comp-bio.pdf"><span class="ep_document_citation">PDF (Author Version)</span></a> - Requires a PDF viewer<br />212Kb</td></tr><tr><td valign="top" style="text-align:center"><a href="http://eprints.utas.edu.au/1559/2/ritar-comp-bio-pub.pdf"><img alt="[img]" src="http://eprints.utas.edu.au/style/images/fileicons/application_pdf.png" class="ep_doc_icon" border="0" /></a></td><td valign="top"><a href="http://eprints.utas.edu.au/1559/2/ritar-comp-bio-pub.pdf"><span class="ep_document_citation">PDF</span></a> - Full text restricted - Requires a PDF viewer<br />372Kb</td></tr></table><p style="margin-bottom: 1em" class="not_ep_block">Official URL: <a href="http://dx.doi.org/10.1016/S1095-6433(03)00167-3">http://dx.doi.org/10.1016/S1095-6433(03)00167-3</a></p><div class="not_ep_block"><h2>Abstract</h2><p style="padding-bottom: 16px; text-align: left; margin: 1em auto 0em auto">We examined biochemical changes accompanying feeding and starvation from hatch to Stage VI (day 74 after hatch) in spiny lobster, Jasus edwardsii, phyllosoma larvae. Larval dry weights (dw) increased 17-fold from hatch (80 +/- 1 ug) to Stage VI (1415 +/- 44 ug). Larvae starved for 6-11 days at Stages II, IV and VI were 14-40% lighter than their fed counterparts fed enriched Artemia. The increases and losses in total dw during feeding and starvation were associated with changes in the content of protein (constituting 31.4-41.7% of dw) and carbohydrate (constituting 2.6-5.3% of dw), while larger changes in lipid content indicated its greater importance as an energy substrate. Lipid content increased from 7.9% of dw at hatch to its highest of 12.5% at Stage IV, but declined by 50% or more during starvation. This suggests that protein, carbohydrate and lipid are all important energy stores, although lipids are catabolised at a greater rate during food deprivation. The principal lipid class was polar lipid (79-92% of total lipid), followed by sterol (6-20%), with triacylglycerol and other lipid classes at <2%. Polar lipids were catabolized and sterols were conserved during starvation. Changes in the fatty acid profile had mostly occurred before the first moult at day 8 after hatch, with gradual changes thereafter to Stage VI, reflecting their abundance in the Artemia diet. There was some conservation of the major essential fatty acids (EFA), 20:4n-6, 20:5n-3, 22:6n-3, and the fatty acid profile showed large gains in the C18 PUFA, 18:1n-9, 18:2n-6. Ascorbic acid content increased 10-fold from hatch to the end of Stage I (36 and 333 ug g-1 dw, respectively), while the content at the end of Stage II was higher in fed than starved larvae (439 and 174 ug g-1 dw, respectively). Our study will assist in the development of alternatives to nutritionally incomplete diets, such as live ongrown Artemia, to meet the requirements of phyllosoma in culture.</p></div><table style="margin-bottom: 1em" cellpadding="3" class="not_ep_block" border="0"><tr><th valign="top" class="ep_row">Item Type:</th><td valign="top" class="ep_row">Article</td></tr><tr><th valign="top" class="ep_row">Additional Information:</th><td valign="top" class="ep_row">The definitive version is available online at http://www.sciencedirect.com/</td></tr><tr><th valign="top" class="ep_row">Keywords:</th><td valign="top" class="ep_row">Artemia; Ascorbic acid; Fatty acid; Jasus edwardsii; Nutrition; Phyllosoma; Spiny lobster; Starvation</td></tr><tr><th valign="top" class="ep_row">Subjects:</th><td valign="top" class="ep_row"><a href="http://eprints.utas.edu.au/view/subjects/300701.html">300000 Agricultural, Veterinary and Environmental Sciences > 300700 Fisheries Sciences > 300701 Physiology and Genetics</a><br /><a href="http://eprints.utas.edu.au/view/subjects/300703.html">300000 Agricultural, Veterinary and Environmental Sciences > 300700 Fisheries Sciences > 300703 Aquaculture</a></td></tr><tr><th valign="top" class="ep_row">ID Code:</th><td valign="top" class="ep_row">1559</td></tr><tr><th valign="top" class="ep_row">Deposited By:</th><td valign="top" class="ep_row"><span class="ep_name_citation"><span class="person_name">Dr Arthur J Ritar</span></span></td></tr><tr><th valign="top" class="ep_row">Deposited On:</th><td valign="top" class="ep_row">09 Aug 2007</td></tr><tr><th valign="top" class="ep_row">Last Modified:</th><td valign="top" class="ep_row">09 Jan 2008 02:30</td></tr><tr><th valign="top" class="ep_row">ePrint Statistics:</th><td valign="top" class="ep_row"><a target="ePrintStats" href="/es/index.php?action=show_detail_eprint;id=1559;">View statistics for this ePrint</a></td></tr></table><p align="right">Repository Staff Only: <a href="http://eprints.utas.edu.au/cgi/users/home?screen=EPrint::View&eprintid=1559">item control page</a></p>
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