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<meta content="Morales, J.F." name="eprints.creators_name" />
<meta content="Snow, Elizabeth T." name="eprints.creators_name" />
<meta content="Murnane, J.P." name="eprints.creators_name" />
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<meta content="Retrotransposons have clearly molded the structure of the human genome. The reverse transcriptase coded for by long interspersed nuclear elements (LINEs) accounts for 35% of the human genome, with 8-9 x 10(5) copies of the most common human LINE element, L1Hs. Retrotransposons cycle through an RNA intermediate with transcription as the rate limiting step. Because various retrotransposons have been demonstrated to be induced by environmental stimuli, we investigated the response of the L1Hs promoter to various agents. L1Hs promoter activity was analyzed by transfecting an L1Hs-expressing cell line with plasmids containing one of two L1Hs promoters fused to the LacZ reporter gene. L1Hs promoter activity was then monitored with a beta-galactosidase assay. Treatment with UV light and heat shock resulted in a small increase in beta-galactosidase activity from one promoter, while treatment with tetradecanoylphorbol 13-acetate resulted in small increases in beta-galactosidase activity from both promoters. No increase in beta-galactosidase activity was observed after exposure to X-rays or hydrogen peroxide." name="eprints.abstract" />
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<meta content="Retrotransposons have clearly molded the structure of the human genome. The reverse transcriptase coded for by long interspersed nuclear elements (LINEs) accounts for 35% of the human genome, with 8-9 x 10(5) copies of the most common human LINE element, L1Hs. Retrotransposons cycle through an RNA intermediate with transcription as the rate limiting step. Because various retrotransposons have been demonstrated to be induced by environmental stimuli, we investigated the response of the L1Hs promoter to various agents. L1Hs promoter activity was analyzed by transfecting an L1Hs-expressing cell line with plasmids containing one of two L1Hs promoters fused to the LacZ reporter gene. L1Hs promoter activity was then monitored with a beta-galactosidase assay. Treatment with UV light and heat shock resulted in a small increase in beta-galactosidase activity from one promoter, while treatment with tetradecanoylphorbol 13-acetate resulted in small increases in beta-galactosidase activity from both promoters. No increase in beta-galactosidase activity was observed after exposure to X-rays or hydrogen peroxide." name="DC.description" />
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<h1 class="ep_tm_pagetitle">Environmental factors affecting transcription of the human L1 retrotransposon. II. Stressors</h1>
<p style="margin-bottom: 1em" class="not_ep_block"><span class="person_name">Morales, J.F.</span> and <span class="person_name">Snow, Elizabeth T.</span> and <span class="person_name">Murnane, J.P.</span> (2003) <xhtml:em>Environmental factors affecting transcription of the human L1 retrotransposon. II. Stressors.</xhtml:em> Mutagenesis, 18 (2). pp. 151-158. ISSN 0267-8357</p><p style="margin-bottom: 1em" class="not_ep_block"></p><table style="margin-bottom: 1em" class="not_ep_block"><tr><td valign="top" style="text-align:center"><a href="http://eprints.utas.edu.au/1789/1/Morales%2CSnow%26Murnane-03.pdf"><img alt="[img]" src="http://eprints.utas.edu.au/style/images/fileicons/application_pdf.png" class="ep_doc_icon" border="0" /></a></td><td valign="top"><a href="http://eprints.utas.edu.au/1789/1/Morales%2CSnow%26Murnane-03.pdf"><span class="ep_document_citation">PDF</span></a> - Full text restricted - Requires a PDF viewer<br />716Kb</td></tr></table><p style="margin-bottom: 1em" class="not_ep_block">Official URL: <a href="http://dx.doi.org/10.1093/mutage/18.2.151">http://dx.doi.org/10.1093/mutage/18.2.151</a></p><div class="not_ep_block"><h2>Abstract</h2><p style="padding-bottom: 16px; text-align: left; margin: 1em auto 0em auto">Retrotransposons have clearly molded the structure of the human genome. The reverse transcriptase coded for by long interspersed nuclear elements (LINEs) accounts for 35% of the human genome, with 8-9 x 10(5) copies of the most common human LINE element, L1Hs. Retrotransposons cycle through an RNA intermediate with transcription as the rate limiting step. Because various retrotransposons have been demonstrated to be induced by environmental stimuli, we investigated the response of the L1Hs promoter to various agents. L1Hs promoter activity was analyzed by transfecting an L1Hs-expressing cell line with plasmids containing one of two L1Hs promoters fused to the LacZ reporter gene. L1Hs promoter activity was then monitored with a beta-galactosidase assay. Treatment with UV light and heat shock resulted in a small increase in beta-galactosidase activity from one promoter, while treatment with tetradecanoylphorbol 13-acetate resulted in small increases in beta-galactosidase activity from both promoters. No increase in beta-galactosidase activity was observed after exposure to X-rays or hydrogen peroxide.</p></div><table style="margin-bottom: 1em" cellpadding="3" class="not_ep_block" border="0"><tr><th valign="top" class="ep_row">Item Type:</th><td valign="top" class="ep_row">Article</td></tr><tr><th valign="top" class="ep_row">Keywords:</th><td valign="top" class="ep_row">LONG-TERMINAL REPEAT; IMMUNODEFICIENCY VIRUS TYPE-1; HUMAN LINE RETROTRANSPOSONS; HUMAN TRANSPOSABLE ELEMENT; HEAT-SHOCK; DROSOPHILA-MELANOGASTER; DNA DAMAGE; IN-VIVO; SACCHAROMYCES-CEREVISIAE; PROCESSED PSEUDOGENES</td></tr><tr><th valign="top" class="ep_row">Subjects:</th><td valign="top" class="ep_row"><a href="http://eprints.utas.edu.au/view/subjects/320504.html">320000 Medical and Health Sciences > 320500 Pharmacology and Pharmaceutical Sciences > 320504 Toxicology (incl. Clinical Toxicology)</a><br /><a href="http://eprints.utas.edu.au/view/subjects/270299.html">270000 Biological Sciences > 270200 Genetics > 270299 Genetics not elsewhere classified</a></td></tr><tr><th valign="top" class="ep_row">ID Code:</th><td valign="top" class="ep_row">1789</td></tr><tr><th valign="top" class="ep_row">Deposited By:</th><td valign="top" class="ep_row"><span class="ep_name_citation"><span class="person_name">Dr Elizabeth T Snow</span></span></td></tr><tr><th valign="top" class="ep_row">Deposited On:</th><td valign="top" class="ep_row">06 Sep 2007</td></tr><tr><th valign="top" class="ep_row">Last Modified:</th><td valign="top" class="ep_row">09 Jan 2008 02:30</td></tr><tr><th valign="top" class="ep_row">ePrint Statistics:</th><td valign="top" class="ep_row"><a target="ePrintStats" href="/es/index.php?action=show_detail_eprint;id=1789;">View statistics for this ePrint</a></td></tr></table><p align="right">Repository Staff Only: <a href="http://eprints.utas.edu.au/cgi/users/home?screen=EPrint::View&eprintid=1789">item control page</a></p>
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