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<meta content="Miller, K.J." name="eprints.creators_name" />
<meta content="Howard, C.G." name="eprints.creators_name" />
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<meta content="Microsatellites were isolated from two broadcast-spawning species of scleractinian coral (Platygyra daedalea and Goniastrea favulus) from Australia's Great Barrier Reef. We found 27 microsatellites across both species, although only five loci were polymorphic in each species. Microsatellite loci displayed a wide range of diversity levels with four to 11 alleles per locus (HO = 0.26–0.91) in P. daedalea and two to seven alleles per locus (HO = 0.16–0.96) in G. favulus. Most loci showed departures from Hardy–Weinberg equilibrium which may reflect nonrandom mating but may also be related to difficulties associated with coral DNA.
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<meta content="flow in brooding and spawning corals along the Great Barrier Reef, Australia. Evolution, 54, 1590–1605.
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•GillingsM, FahyP (1993) Genetic diversity of Pseudomonas solanacearum biovars 2 and N2 assessed using restriction endonuclease analysis of total genomic DNA. Plant Pathology, 42, 744–753.
Synergy, ISI
•HamiltonMB, PincusEL, DiFioreA, FleischerRC (1999) Universal linker and ligation procedures for construction of genomic DNA libraries enriched for microsatellites. Biotechniques, 27, 500–507.
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•HauswaldtJS, GlennTC (2003) Microsatellite DNA loci from the Diamondback terrapin (Malaclemys terrapin). Molecular Ecology Notes, 3 (2), 174–176.
Synergy, ISI, CSA
•HeywardAJ, BabcockRC (1986) Self- and cross-fertilization in scleractinian corals. Marine Biology, 90, 191–195.
CrossRef, ISI, CSA
•MarquezLM, MacKenzieJB, TakabayashiM, SmithCR, ChenCA (2003) Difficulties in obtaining microsatellites from acroporid corals. Proceedings of the 9th Coral Reef Symposium, 1, 139–143.
•MillerK, MundyC (2003) Rapid settlement in broadcast spawning corals: implications for larval dispersal. Coral Reefs, 22, 99–106.
CrossRef, ISI, CSA
•MillerKJ, BabcockRC (1997) Conflicting morphological and reproductive species boundaries in the scleractinian coral genus Platygyra. Biology Bulletin, 192, 98–110.
ISI, CSA
•MillerKJ, BenzieJAH (1997) No clear genetic distinction between morphological species within the coral genus Platygyra. Bulletin of Marine Science, 60, 907–917.
•NozawaY, HarrisonPL (2003) Larval settlement patterns, dispersal potential, and the effect of temperature on settlement of larvae of the reef coral, Platygyra daedalea, from the Great Barrier Reef. Proceedings of the 9th International Coral Reef Symposium, 1, 409–415.
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<meta content="Microsatellites were isolated from two broadcast-spawning species of scleractinian coral (Platygyra daedalea and Goniastrea favulus) from Australia's Great Barrier Reef. We found 27 microsatellites across both species, although only five loci were polymorphic in each species. Microsatellite loci displayed a wide range of diversity levels with four to 11 alleles per locus (HO = 0.26–0.91) in P. daedalea and two to seven alleles per locus (HO = 0.16–0.96) in G. favulus. Most loci showed departures from Hardy–Weinberg equilibrium which may reflect nonrandom mating but may also be related to difficulties associated with coral DNA.
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<h1 class="ep_tm_pagetitle">Isolation of microsatellites for two species of scleractinian coral</h1>
<p style="margin-bottom: 1em" class="not_ep_block"><span class="person_name">Miller, K.J.</span> and <span class="person_name">Howard, C.G.</span> (2004) <xhtml:em>Isolation of microsatellites for two species of scleractinian coral.</xhtml:em> Molecular Ecology Notes, 4 (1). pp. 11-13. ISSN 1471-8278</p><p style="margin-bottom: 1em" class="not_ep_block"></p><table style="margin-bottom: 1em" class="not_ep_block"><tr><td valign="top" style="text-align:center"><a href="http://eprints.utas.edu.au/2168/1/isolation_microsatellites.pdf"><img alt="[img]" src="http://eprints.utas.edu.au/style/images/fileicons/application_pdf.png" class="ep_doc_icon" border="0" /></a></td><td valign="top"><a href="http://eprints.utas.edu.au/2168/1/isolation_microsatellites.pdf"><span class="ep_document_citation">PDF</span></a> - Full text restricted - Requires a PDF viewer<br />76Kb</td><td><form method="get" accept-charset="utf-8" action="http://eprints.utas.edu.au/cgi/request_doc"><input accept-charset="utf-8" value="2710" name="docid" type="hidden" /><div class=""><input value="Request a copy" name="_action_null" class="ep_form_action_button" onclick="return EPJS_button_pushed( '_action_null' )" type="submit" /> </div></form></td></tr></table><p style="margin-bottom: 1em" class="not_ep_block">Official URL: <a href="http://dx.doi.org/10.1046/j.1471-8286.2003.00555.x">http://dx.doi.org/10.1046/j.1471-8286.2003.00555.x</a></p><div class="not_ep_block"><h2>Abstract</h2><p style="padding-bottom: 16px; text-align: left; margin: 1em auto 0em auto">Microsatellites were isolated from two broadcast-spawning species of scleractinian coral (Platygyra daedalea and Goniastrea favulus) from Australia's Great Barrier Reef. We found 27 microsatellites across both species, although only five loci were polymorphic in each species. Microsatellite loci displayed a wide range of diversity levels with four to 11 alleles per locus (HO = 0.26–0.91) in P. daedalea and two to seven alleles per locus (HO = 0.16–0.96) in G. favulus. Most loci showed departures from Hardy–Weinberg equilibrium which may reflect nonrandom mating but may also be related to difficulties associated with coral DNA.
</p></div><table style="margin-bottom: 1em" cellpadding="3" class="not_ep_block" border="0"><tr><th valign="top" class="ep_row">Item Type:</th><td valign="top" class="ep_row">Article</td></tr><tr><th valign="top" class="ep_row">Additional Information:</th><td valign="top" class="ep_row">The definitive version is available at www.blackwell-synergy.com
</td></tr><tr><th valign="top" class="ep_row">Keywords:</th><td valign="top" class="ep_row">Cnidaria, coral, Goniastrea favulus, microsatellites, Platygyra daedalea, population genetics </td></tr><tr><th valign="top" class="ep_row">Subjects:</th><td valign="top" class="ep_row"><a href="http://eprints.utas.edu.au/view/subjects/270203.html">270000 Biological Sciences > 270200 Genetics > 270203 Population and Ecological Genetics</a></td></tr><tr><th valign="top" class="ep_row">ID Code:</th><td valign="top" class="ep_row">2168</td></tr><tr><th valign="top" class="ep_row">Deposited By:</th><td valign="top" class="ep_row"><span class="ep_name_citation"><span class="person_name">Dr Karen Miller</span></span></td></tr><tr><th valign="top" class="ep_row">Deposited On:</th><td valign="top" class="ep_row">15 Oct 2007 14:58</td></tr><tr><th valign="top" class="ep_row">Last Modified:</th><td valign="top" class="ep_row">09 Jan 2008 02:30</td></tr><tr><th valign="top" class="ep_row">ePrint Statistics:</th><td valign="top" class="ep_row"><a target="ePrintStats" href="/es/index.php?action=show_detail_eprint;id=2168;">View statistics for this ePrint</a></td></tr></table><p align="right">Repository Staff Only: <a href="http://eprints.utas.edu.au/cgi/users/home?screen=EPrint::View&eprintid=2168">item control page</a></p>
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