<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" "http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd"> <html> <head> <title>UTas ePrints - Substantial allozyme diversity in the freshwater crayfish Parastacoides tasmanicus supports extensive cryptic speciation</title> <script type="text/javascript" src="http://eprints.utas.edu.au/javascript/auto.js"><!-- padder --></script> <style type="text/css" media="screen">@import url(http://eprints.utas.edu.au/style/auto.css);</style> <style type="text/css" media="print">@import url(http://eprints.utas.edu.au/style/print.css);</style> <link rel="icon" href="/images/eprints/favicon.ico" type="image/x-icon" /> <link rel="shortcut icon" href="/images/eprints/favicon.ico" type="image/x-icon" /> <link rel="Top" href="http://eprints.utas.edu.au/" /> <link rel="Search" href="http://eprints.utas.edu.au/cgi/search" /> <meta content="Hansen, Brita" name="eprints.creators_name" /> <meta content="Krasnicki, Tom" name="eprints.creators_name" /> <meta content="Richardson, A.M.M." name="eprints.creators_name" /> <meta content="" name="eprints.creators_id" /> <meta content="" name="eprints.creators_id" /> <meta content="Alastair.Richardson@utas.edu.au" name="eprints.creators_id" /> <meta content="article" name="eprints.type" /> <meta content="2007-10-22 21:56:26" name="eprints.datestamp" /> <meta content="2008-01-08 15:30:00" name="eprints.lastmod" /> <meta content="show" name="eprints.metadata_visibility" /> <meta content="Substantial allozyme diversity in the freshwater crayfish Parastacoides tasmanicus supports extensive cryptic speciation" name="eprints.title" /> <meta content="pub" name="eprints.ispublished" /> <meta content="270000" name="eprints.subjects" /> <meta content="restricted" name="eprints.full_text_status" /> <meta content="Allozyme electrophoretic studies on the freshwater crayfish genus Parastacoides Clark suggest the presence of several cryptic species within this morphologically conservative taxon. Two independent allozyme studies were undertaken to assess the validity of the current taxonomy of this monotypic genus. An initial study examined 42 individuals from 10 sample sets for allozyme variation at 22 putative loci, and a subsequent study surveyed an additional 72 specimens from 20 sample sets at 16 putative loci. Both studies revealed the same general outcomes, namely: (1) several instances of sympatric species diagnosable at multiple allozyme loci; (2) numerous examples of putative allopatric species with significant levels of genetic divergence (25–81% fixed difference, 0.30–1.67 Nei distance) well beyond those found between conspecific populations of any parastacid; (3) broad genetic affinities among putative species are inconsistent with currently recognised morphotypes; and (4) low levels of within-population genetic variability, typical of parastacids. Although it is not possible to determine how many species are represented on the basis of these two preliminary studies, the allozyme data nevertheless indicate that an absolute minimum of 11 species and perhaps as many as 19 species are likely to be present in the genus. They indicate the need for a thorough taxonomic revision of the genus using both molecular and morphological data." name="eprints.abstract" /> <meta content="2001" name="eprints.date" /> <meta content="published" name="eprints.date_type" /> <meta content="Invertebrate Systematics" name="eprints.publication" /> <meta content="15" name="eprints.volume" /> <meta content="5" name="eprints.number" /> <meta content="667-679" name="eprints.pagerange" /> <meta content="TRUE" name="eprints.refereed" /> <meta content="http://www.publish.csiro.au/index.cfm" name="eprints.official_url" /> <meta content="Allibone, R. M., Crowl, T. A., Holmes, J. M., King, T. M., McDowall, R. M. Townsend, C. R., and Wallis, G. P. (1996). Isozyme analysis of Galaxias species from the Taieri River, South Island, New Zealand: a species complex revealed. Biological Journal of the Linnean Society 57, 107–127. Austin, C. M. (1996). Systematics of the freshwater crayfish genus Cherax Erichson (Decapoda: Parastacidae) in northern and eastern Australia: electrophoretic and morphological variation. Australian Journal of Zoology 44, 259–296. Austin, C. M., and Knott, B. (1996). Systematics of the freshwater crayfish genus Cherax Erichson (Decapoda: Parastacidae) in southwestern Australia: electrophoretic, morphological and habitat variation. Australian Journal of Zoology 44, 223–258. Avery, L., and Austin, C. M. (1997). A biochemical taxonomic study of spiny crayfish of the genera Astacopsis and Euastacus (Decapoda: Parastacidae) in south-eastern Australia. Memoirs of the Museum of Victoria 56, 543–555. Avise, J. C. (1994). ‘Molecular Markers, Natural History and Evolution.’ (Chapman and Hall: New York.) Ayala, F. J. (1982). ‘Population and Evolutionary Genetics: a Primer.’ (The Benjamin Cummings Publishing Company, Inc.: Menlo Park, California.) Baverstock, P. R., and Adams, M. (1987). Rates of molecular, chromosomal and morphological evolution in some Australian vertebrates. In ‘Rates of Evolution’. (Eds K. S. W. Campbell and M. F. Day.) pp. 175-188. (Allen and Unwin: London.) Baverstock, P. R., Watts, C. H. S., and Cole, S. R. (1977). Electrophoretic comparisons between allopatric populations of five Australian Pseudomyine rodents. Australian Journal of Biological Science 30, 471–485. Baverstock, P. R., Watts, C. H. S., Adams, M., and Gelder, M. (1980). Chromosomal and electrophoretic studies of Australian Melomys (Rodentia: Muridae). Australian Journal of Zoology 28, 553–574. Brown, K. (1980). Low genetic variability and high similarities in the crayfish genera Cambarus and Procambarus. American Midland Naturalist 105, 225–232. Campbell, N. J. H., Geddes, M. C., and Adams, M. (1994). Genetic variation in yabbies (Cherax destructor Clark and C. albidus Clark (Crustacea: Decapoda: Parastacidae)) indicates the presence of a single, highly sub-structured, species. Australian Journal of Zoology 42, 745–760. Clark, E. (1936). The freshwater and land crayfishes of Australia. Memoirs of the National Museum of Victoria 10, 5–58. Clark, E. (1939). Tasmanian Parasticidae. Proceedings of the Royal Society of Tasmania 1938, 117–127. Clark, E. (1941). New species of Australian freshwater and land crayfishes (Family Parastacidae). Memoirs of the National Museum of Victoria 12, 31–40. Fetzner, J. W. Jr. (1996). Biochemical systematics and evolution of the crayfish genus Oronectes (Decapoda: Cambaridae). Journal of Crustacean Biology 16(1), 111–141. Fisher, R. A., and Yates, F. (1963). ‘Statistical Tables for Biological, Agricultural and Medical Research.’ 6th Ed. (Oliver and Boyd: Edinburgh.) Frost, D. R., and Hillis, D. M. (1990). Species in concept and practice: herpetological applications. Herpetologica 46, 87–104. Highton, R. (1989). Biochemical evolution in the slimy salamanders of the Plethodon glutinosus complex in the eastern United States. Part I. Geographic protein variation. University of Illinois Biological Monographs 57, 1 –78. Hillis, D. M., Mabel, B. K., and Moritz, C. (1996). Applications of molecular systematics. In ‘Molecular Systematics’. (Eds D. M. Hillis, C. Moritz, and B. K. Mabel.) pp. 515–543. (Sinauer Associates: Sunderland, Massachussetts.) Allozyme Horwitz, P. (1989). The faunal assemblage (or pholeteros) of some freshwater burrows in southwest Tasmania. Bulletin of the Australian Society of Limnology 12, 29–36. Horwitz, P. (1990). A taxonomic revision of species in the freshwater crayfish genus Engaeus Erichson (Decapoda : Parastacidae). Invertebrate Taxonomy 4, 427–614. Horwitz, P., Adams, M., and Baverstock, P. (1990). Electrophoretic contributions to the systematics of the freshwater crayfish genus Engaeus Erichson (Decapoda: Parastacidae). Invertebrate Taxonomy 4, 615–641. Hull, D. L. (1984). Contemporary systematic philosophies. In ‘Conceptual Issues in Evolutionary Biology’. (Ed. E. Sober) pp. 567–602. (Massachusetts Institute of Technology: Cambridge, Massachusetts.) Lake, P. S. (1977). Pholeteros – the faunal assemblage found in crayfish burrows. Australian Society of Limnology Newsletter 15, 57–60. Mayr, E. (1969). ‘Principles of Systematic Zoology.’ (McGraw-Hill: New York.) Nei, M. (1978). Estimation of average heterozygosity and genetic distance from a small number of individuals. Genetics 89, 583–590. Nemeth, S. T., and Tracey, M. L. (1979). Allozyme variability and relatedness in six crayfish species. The Journal of Heredity 70, 37–43. Richardson, A. M. M. (1983). The effects of the burrows of a crayfish on the respiration of the surrounding soil. Soil Biology and Biochemistry 15(3), 239–242. Richardson, A. M. M., and Horwitz, P. H. J. (1988). Habitat partitioning in parastacid crayfish. Freshwater Crayfish 7, 91–97. Richardson, A. M. M., and Wong, V. (1995). The effect of a burrowing crayfish, Parastacoides sp., on the vegetation of Tasmanian wet heathlands. Freshwater Crayfish 10, 174–182. Richardson, B. J., Baverstock, P. R., and Adams, M. (1986). ‘Allozyme Electrophoresis: a Handbook for Animal Systematics and Population Studies.’ (Academic Press: Sydney.) Riek, E. F. (1951). The freshwater crayfish (Family Parastacidae) of Queensland. Records of the Australian Museum 22, 368–388. Riek, E. F. (1967). The Tasmanian freshwater crayfish genus Parastacoides (Decapoda : Parastacidae). Australian Journal of Zoology 15, 999–1006. Riek, E. F. (1969). The Australian freshwater crayfish (Crustacea : Decapoda : Parastacidae), with descriptions of new species. Australian Journal of Zoology 17, 855-918. Riek, E.F. (1972). The phylogeny of the Parastacidae (Crustacea : Astacoidea), and description of a new genus of Australian freshwater crayfishes. Australian Journal of Zoology 20, 369–389. Sokal, R. R., and Sneath, P. H. A. (1963). ‘The Principles of Numerical Taxonomy.’ (W. H. Freeman and Company: San Francisco.) Sumner, C. E. (1978). A revision of the genus Parastacoides Clark (Crustacea: Decapoda: Parastacidae). Australian Journal of Zoology 26, 809–821. Swofford, D. L., and Selander, R. B. (1981). ‘BIOSYS-1. A Computer Program for the Analysis of Allelic Variation in Population Genetics and Biochemical Systematics. Release 1. 8.’ (Illinois Natural History Survey: Champaign, Illinois.) Thorpe, J. P. (1982). The molecular clock hypothesis: biochemical evolution, genetic differentiation and systematics. Annual Review of Ecological Systematics 13, 139–168. Zeidler, W., and Adams, M. (1990). Revision of the Australian genus of freshwater crayfish Gramastacus Riek (Decapoda, Parastacidae). Invertebrate Taxonomy 3, 913–924. Manuscript received 8 December 2000; revised and accepted 25 May 2001." name="eprints.referencetext" /> <meta content="Hansen, Brita and Krasnicki, Tom and Richardson, A.M.M. (2001) Substantial allozyme diversity in the freshwater crayfish Parastacoides tasmanicus supports extensive cryptic speciation. Invertebrate Systematics, 15 (5). pp. 667-679." name="eprints.citation" /> <meta content="http://eprints.utas.edu.au/2292/1/AllozymeInvTax.pdf" name="eprints.document_url" /> <link rel="schema.DC" href="http://purl.org/DC/elements/1.0/" /> <meta content="Substantial allozyme diversity in the freshwater crayfish Parastacoides tasmanicus supports extensive cryptic speciation" name="DC.title" /> <meta content="Hansen, Brita" name="DC.creator" /> <meta content="Krasnicki, Tom" name="DC.creator" /> <meta content="Richardson, A.M.M." name="DC.creator" /> <meta content="270000 Biological Sciences" name="DC.subject" /> <meta content="Allozyme electrophoretic studies on the freshwater crayfish genus Parastacoides Clark suggest the presence of several cryptic species within this morphologically conservative taxon. Two independent allozyme studies were undertaken to assess the validity of the current taxonomy of this monotypic genus. An initial study examined 42 individuals from 10 sample sets for allozyme variation at 22 putative loci, and a subsequent study surveyed an additional 72 specimens from 20 sample sets at 16 putative loci. Both studies revealed the same general outcomes, namely: (1) several instances of sympatric species diagnosable at multiple allozyme loci; (2) numerous examples of putative allopatric species with significant levels of genetic divergence (25–81% fixed difference, 0.30–1.67 Nei distance) well beyond those found between conspecific populations of any parastacid; (3) broad genetic affinities among putative species are inconsistent with currently recognised morphotypes; and (4) low levels of within-population genetic variability, typical of parastacids. Although it is not possible to determine how many species are represented on the basis of these two preliminary studies, the allozyme data nevertheless indicate that an absolute minimum of 11 species and perhaps as many as 19 species are likely to be present in the genus. They indicate the need for a thorough taxonomic revision of the genus using both molecular and morphological data." name="DC.description" /> <meta content="2001" name="DC.date" /> <meta content="Article" name="DC.type" /> <meta content="PeerReviewed" name="DC.type" /> <meta content="application/pdf" name="DC.format" /> <meta content="http://eprints.utas.edu.au/2292/1/AllozymeInvTax.pdf" name="DC.identifier" /> <meta content="http://www.publish.csiro.au/index.cfm" name="DC.relation" /> <meta content="Hansen, Brita and Krasnicki, Tom and Richardson, A.M.M. (2001) Substantial allozyme diversity in the freshwater crayfish Parastacoides tasmanicus supports extensive cryptic speciation. 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border: solid 1px #ccc; padding: 3px"><tr> <td align="left"><a href="http://eprints.utas.edu.au/cgi/users/home">Login</a> | <a href="http://eprints.utas.edu.au/cgi/register">Create Account</a></td> <td align="right" style="white-space: nowrap"> <form method="get" accept-charset="utf-8" action="http://eprints.utas.edu.au/cgi/search" style="display:inline"> <input class="ep_tm_searchbarbox" size="20" type="text" name="q" /> <input class="ep_tm_searchbarbutton" value="Search" type="submit" name="_action_search" /> <input type="hidden" name="_order" value="bytitle" /> <input type="hidden" name="basic_srchtype" value="ALL" /> <input type="hidden" name="_satisfyall" value="ALL" /> </form> </td> </tr></table></td></tr> <tr> <td class="toplinks"><!-- InstanceBeginEditable name="content" --> <div align="center"> <table width="720" class="ep_tm_main"><tr><td align="left"> <h1 class="ep_tm_pagetitle">Substantial allozyme diversity in the freshwater crayfish Parastacoides tasmanicus supports extensive cryptic speciation</h1> <p style="margin-bottom: 1em" class="not_ep_block"><span class="person_name">Hansen, Brita</span> and <span class="person_name">Krasnicki, Tom</span> and <span class="person_name">Richardson, A.M.M.</span> (2001) <xhtml:em>Substantial allozyme diversity in the freshwater crayfish Parastacoides tasmanicus supports extensive cryptic speciation.</xhtml:em> Invertebrate Systematics, 15 (5). pp. 667-679.</p><p style="margin-bottom: 1em" class="not_ep_block"></p><table style="margin-bottom: 1em" class="not_ep_block"><tr><td valign="top" style="text-align:center"><a href="http://eprints.utas.edu.au/2292/1/AllozymeInvTax.pdf"><img alt="[img]" src="http://eprints.utas.edu.au/style/images/fileicons/application_pdf.png" class="ep_doc_icon" border="0" /></a></td><td valign="top"><a href="http://eprints.utas.edu.au/2292/1/AllozymeInvTax.pdf"><span class="ep_document_citation">PDF</span></a> - Full text restricted - Requires a PDF viewer<br />525Kb</td><td><form method="get" accept-charset="utf-8" action="http://eprints.utas.edu.au/cgi/request_doc"><input accept-charset="utf-8" value="2928" name="docid" type="hidden" /><div class=""><input value="Request a copy" name="_action_null" class="ep_form_action_button" onclick="return EPJS_button_pushed( '_action_null' )" type="submit" /> </div></form></td></tr></table><p style="margin-bottom: 1em" class="not_ep_block">Official URL: <a href="http://www.publish.csiro.au/index.cfm">http://www.publish.csiro.au/index.cfm</a></p><div class="not_ep_block"><h2>Abstract</h2><p style="padding-bottom: 16px; text-align: left; margin: 1em auto 0em auto">Allozyme electrophoretic studies on the freshwater crayfish genus Parastacoides Clark suggest the presence of several cryptic species within this morphologically conservative taxon. Two independent allozyme studies were undertaken to assess the validity of the current taxonomy of this monotypic genus. An initial study examined 42 individuals from 10 sample sets for allozyme variation at 22 putative loci, and a subsequent study surveyed an additional 72 specimens from 20 sample sets at 16 putative loci. Both studies revealed the same general outcomes, namely: (1) several instances of sympatric species diagnosable at multiple allozyme loci; (2) numerous examples of putative allopatric species with significant levels of genetic divergence (25–81% fixed difference, 0.30–1.67 Nei distance) well beyond those found between conspecific populations of any parastacid; (3) broad genetic affinities among putative species are inconsistent with currently recognised morphotypes; and (4) low levels of within-population genetic variability, typical of parastacids. Although it is not possible to determine how many species are represented on the basis of these two preliminary studies, the allozyme data nevertheless indicate that an absolute minimum of 11 species and perhaps as many as 19 species are likely to be present in the genus. They indicate the need for a thorough taxonomic revision of the genus using both molecular and morphological data.</p></div><table style="margin-bottom: 1em" cellpadding="3" class="not_ep_block" border="0"><tr><th valign="top" class="ep_row">Item Type:</th><td valign="top" class="ep_row">Article</td></tr><tr><th valign="top" class="ep_row">Subjects:</th><td valign="top" class="ep_row"><a href="http://eprints.utas.edu.au/view/subjects/270000.html">270000 Biological Sciences</a></td></tr><tr><th valign="top" class="ep_row">ID Code:</th><td valign="top" class="ep_row">2292</td></tr><tr><th valign="top" class="ep_row">Deposited By:</th><td valign="top" class="ep_row"><span class="ep_name_citation"><span class="person_name">A/Prof Alastair Richardson</span></span></td></tr><tr><th valign="top" class="ep_row">Deposited On:</th><td valign="top" class="ep_row">23 Oct 2007 08:56</td></tr><tr><th valign="top" class="ep_row">Last Modified:</th><td valign="top" class="ep_row">09 Jan 2008 02:30</td></tr><tr><th valign="top" class="ep_row">ePrint Statistics:</th><td valign="top" class="ep_row"><a target="ePrintStats" href="/es/index.php?action=show_detail_eprint;id=2292;">View statistics for this ePrint</a></td></tr></table><p align="right">Repository Staff Only: <a href="http://eprints.utas.edu.au/cgi/users/home?screen=EPrint::View&eprintid=2292">item control page</a></p> </td></tr></table> </div> <!-- InstanceEndEditable --></td> </tr> <tr> <td><!-- #BeginLibraryItem "/Library/footer_eprints.lbi" --> <table width="795" border="0" align="left" cellpadding="0" class="footer"> <tr valign="top"> <td colspan="2"><div align="center"><a href="http://www.utas.edu.au">UTAS home</a> | <a href="http://www.utas.edu.au/library/">Library home</a> | <a href="/">ePrints home</a> | <a href="/contact.html">contact</a> | <a href="/information.html">about</a> | <a href="/view/">browse</a> | <a href="/perl/search/simple">search</a> | <a href="/perl/register">register</a> | <a href="/perl/users/home">user area</a> | <a href="/help/">help</a></div><br /></td> </tr> <tr><td colspan="2"><p><img src="/images/eprints/footerline.gif" width="100%" height="4" /></p></td></tr> <tr valign="top"> <td width="68%" class="footer">Authorised by the University Librarian<br /> © University of Tasmania ABN 30 764 374 782<br /> <a href="http://www.utas.edu.au/cricos/">CRICOS Provider Code 00586B</a> | <a href="http://www.utas.edu.au/copyright/copyright_disclaimers.html">Copyright & Disclaimers</a> | <a href="http://www.utas.edu.au/accessibility/index.html">Accessibility</a> | <a href="http://eprints.utas.edu.au/feedback/">Site Feedback</a> </td> <td width="32%"><div align="right"> <p align="right" class="NoPrint"><a href="http://www.utas.edu.au/"><img src="http://www.utas.edu.au/shared/logos/unioftasstrip.gif" alt="University of Tasmania Home Page" width="260" height="16" border="0" align="right" /></a></p> <p align="right" class="NoPrint"><a href="http://www.utas.edu.au/"><br /> </a></p> </div></td> </tr> <tr valign="top"> <td><p> </p></td> <td><div align="right"><span class="NoPrint"><a href="http://www.eprints.org/software/"><img src="/images/eprintslogo.gif" alt="ePrints logo" width="77" height="29" border="0" align="bottom" /></a></span></div></td> </tr> </table> <!-- #EndLibraryItem --> <div align="center"></div></td> </tr> </table> </body> </html>