<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" "http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd"> <html> <head> <title>UTas ePrints - Control of Gibberellin Levels and Gene Expression during De-Etiolation in Pea</title> <script type="text/javascript" src="http://eprints.utas.edu.au/javascript/auto.js"><!-- padder --></script> <style type="text/css" media="screen">@import url(http://eprints.utas.edu.au/style/auto.css);</style> <style type="text/css" media="print">@import url(http://eprints.utas.edu.au/style/print.css);</style> <link rel="icon" href="/images/eprints/favicon.ico" type="image/x-icon" /> <link rel="shortcut icon" href="/images/eprints/favicon.ico" type="image/x-icon" /> <link rel="Top" href="http://eprints.utas.edu.au/" /> <link rel="Search" href="http://eprints.utas.edu.au/cgi/search" /> <meta content="Reid, James B." name="eprints.creators_name" /> <meta content="Botwright, Natasha A." name="eprints.creators_name" /> <meta content="Smith, Jennifer J." name="eprints.creators_name" /> <meta content="O'Neill, Damian P." name="eprints.creators_name" /> <meta content="Kerckhoffs, L. Huub J." name="eprints.creators_name" /> <meta content="James.Reid@utas.edu.au" name="eprints.creators_id" /> <meta content="" name="eprints.creators_id" /> <meta content="" name="eprints.creators_id" /> <meta content="" name="eprints.creators_id" /> <meta content="" name="eprints.creators_id" /> <meta content="article" name="eprints.type" /> <meta content="2007-10-30 21:51:14" name="eprints.datestamp" /> <meta content="2008-01-08 15:30:00" name="eprints.lastmod" /> <meta content="show" name="eprints.metadata_visibility" /> <meta content="Control of Gibberellin Levels and Gene Expression during De-Etiolation in Pea" name="eprints.title" /> <meta content="pub" name="eprints.ispublished" /> <meta content="320000" name="eprints.subjects" /> <meta content="restricted" name="eprints.full_text_status" /> <meta content="Gibberellin Al (GAl) levels drop significantly in wild-type pea (Pisum sativum) plants within 4 h of exposure to red, blue, or far-red light. This response is controlled by phytochrome A (phyA) (and not phyB) and a blue light receptor. GAs levels are increased in response to 4 h of red light, whereas the levels of GA19, GA2o, and GA29 do not vary substantially. Red light appears to control GAl levels by down-regulating the expression of Mendel's LE (PsGA30xl) gene that controls the conversion of GA20 to GAl' and by up-regulating PsGA2ox2, which codes for a GA 2-oxidase that converts GAl to GAs. This occurs within 0.5 to 1 h of exposure to red light. Similar responses occur in blue light. The major GA 20-oxidase gene expressed in shoots, PsGA20oxl, does not show substantial light regulation, but does show up-regulation after 4 h of red light, probably as a result of feedback regulation. Expression of PsGA30xl shows a similar feedback response, whereas PsGA2ox2 shows a feed-forward response. These results add to our understanding of how light reduces shoot elongation during de-etiolation." name="eprints.abstract" /> <meta content="2002" name="eprints.date" /> <meta content="published" name="eprints.date_type" /> <meta content="Plant Physiology" name="eprints.publication" /> <meta content="128" name="eprints.volume" /> <meta content="2" name="eprints.number" /> <meta content="734-741" name="eprints.pagerange" /> <meta content="TRUE" name="eprints.refereed" /> <meta content="0032-0889" name="eprints.issn" /> <meta content="http://proquest.umi.com/pqdweb?did=109212662&sid=1&Fmt=4&clientId=20931&RQT=309&VName=PQD" name="eprints.official_url" /> <meta content="Ausubel FM, Brent R, Kingston RE, Moore DD, Seidman JG, Smith JA, Struhl K (1994) Current Protocols in Molecular Biology, Vol 1. Wiley Interscience, New York Ait-Ali T, Frances S, Weller JL, Reid JB, Kendrick RE, Kamiya Y (1999) Regulation of gibberellin 20-oxidase and gibberellin 3{3-hydroxylase transcript accumulation during de-etiolation of pea seedlings. Plant Physiol 121: 783-791 Ait-Ali T, Swain SM, Reid JB, Sun T, Kamiya Y (1997)The LS locus of pea encodes the gibberellin biosynthesis enzyme ent-kaurene synthase A. Plant J 11: 443-454 Behringer FJ, Davies PJ, Reid JB (1992) Phytochrome regulation of stern growth and indole-3-acetic acid levels in the Iv and Lv genotypes of Pisum. Photochem Photobiol 56: 677-684 Elliott RC, Ross JJ, Smith JJ, Lester DR, Reid JB (2001) Feed-forward regulation of gibberellin deactivation in pea. J Plant Growth Regul 20: 87-94 Garcia-Martinez JL, Lopez-Diaz I, Sanchez-Beltran MJ, Phillips AL, Ward DA, Gaskin P, Hedden P (1997) Isolation and transcript analysis of gibberellin 20-oxidase genes in pea and bean in relation to fruit development. Plant Mol BioI 33: 1073-1084 Gil J, Garcia-Martinez JL (2000) Light regulation of gibberellin A(l) content and expression of genes coding for GA 20-oxidase and GA 3 {3-hydroxylase in etiolated pea seedlings. Physiol Plant 180: 223-229 Hedden P, Phillips AL (2000) Gibberellin metabolism: new insights revealed by the genes. Trends Plant Sci 5: 523-530 Hisada A, Hanzawa H, Weller JL, Nagatani A, Reid JB, Furuya M (2000) Light-induced nuclear translocation of endogenous pea phytochrome A visualized by immunocytochemical procedures. Plant Cell 12: 1063-1078 Jackson SD, James PE, Carrera E, Prat S, Thomas B (2000) Regulation of transcript levels of a potato gibberellin 20-oxidase gene by light and phytochrome B. Plant Physiol 124: 423-430 Kamiya Y, Garcia-Martinez JL (1999) Regulation of gibberellin biosynthesis by light. Curr Opin Plant Biol 2: 398-403 Kende H, Lang A (1964) Gibberellins and light inhibition of stern growth in peas. Plant Physiol 39: 435-440 Lester DR, Ross JJ, Davies PJ, Reid JB (1997) Mendel's stern length gene (Le) encodes a gibberellin 3{3hydroxylase. Plant Cell 9: 1435-1443 Lester DR, Ross JJ, Smith JJ, Elliott RC, Reid JB (1999) Gibberellin 2-oxidation and the SLN gene of Pisum sativum. Plant J 19: 1-9 Lockhart JA (1956) The effect of light and the gibberellins on stern elongation in dwarf and normal pea seedlings. Plant Physiol 31: xii Martin DN, Proebsting WM, Parks TD, Dougherty WG, Lange T, Lewis MJ, Gaskin P, Hedden P (1996) Feed- back regulation of gibberellin biosynthesis and gene expression in Pisum sativum 1. Planta 200: 159-166 O'Neill DP, Ross H, Reid JB (2000) Changes in gibberellin Al levels and response during de-etiolation of pea seedlings. Plant Physiol 124: 805-812 Parks BM, Spalding EP (1999) Sequential and coordinated action of phytochromes A and B during Arabidopsis stern growth revealed by kinetic analysis. Proc Nat! Acad Sci USA 96: 14142-14146 Reed JW, Nagatani A, Chory J, Furuya M (1994) Phytochrome A and phytochrome B have overlapping but distinct functions in Arabidopsis development. Plant Physiol 104: 1139-1149 Reid JB (1983) Internode length in Pisum. Do the internode length genes effect growth in dark-grown plants. Plant Physiol 72: 759-763 Reid JB (1988) Internode length in Pisum: comparison of genotypes in the light and dark. Physiol Plant 74: 83-88 Reid JB, Ross H (1988) A new gene, lv, conferring an enhanced response to gibberellin AI. Physiol Plant 72: 595-604 Reid JB, Ross H, Swain SM (1992) Internode length in Pisum: a new, slender mutant with elevated levels of CI 9 gibberellins. Planta 188: 462-467 Ross H, MacKenzie-Hose AK, Davies PJ, Lester DR, Twitchin B, Reid JB (1999) Further evidence for feedback regulation of gibberellin biosynthesis in pea. Physiol Plant 105: 532-538 Ross H, Reid JB, Swain SM, Hasan 0, Poole AT, Hedden P, Willis CL (1995) Genetic regulation of Gibberellin deactivation in Pisum. Plant J 7: 513-523 Ross H, Willis CL, Gaskin P, Reid JB (1992) Shoot elongation in Lnthyrus odoratus 1.: gibberellin levels in light Gibberellin Levels and Gene Expression during De-Etiolation and dark-grown tall and dwarf seedlings. Planta 187: 10-13 Talon M, Zeevaart JAD, Gage DA (1991) Identification of gibberellins in spinach and effects of light and darkness on their levels. Plant Physiol 97: 1521-1526 Toyomasu T, Kawaide H, Mitsuhashi W, Inoue Y, Kamiya Y (1998) Phytochrome regulates gibberellin biosynthesis during germination of photoblastic lettuce seeds. Plant Physiol 118: 1517-1523 Weller JL, Beauchamp N, Kerckhoffs LHJ, Platten JD, Reid JB (2001) Interaction of phytochromes A and B in the control of de-etiolation and flowering in pea. Plant J 26: 1-14 Weller JL, Murfet IC, Reid JB (1997) Pea mutants with reduced sensitivity to far-red light define an important role for phytochrome A in day-length detection. Plant Physiol 114: 1225-1236 Weller JL, Nagatani A, Kendrick RE, Murfet IC, Reid JB (1995) New lu mutants of pea are deficient in phytochrome B. Plant Physiol 108: 525-532 Weller JL, Ross H, Reid JB' (1994) Gibberellins and phytochrome regulation of stem elongation in pea. Planta 192: 489-496 Wu K, GageAD,ZeevaartJAD (1996)Molecularcloning and photoperiod-regulated expression of gibberellin20-oxidase from long-day plant spinach. Plant Physiol110: 547-554 Xu X, Van Lammeren AM, Vermeer E, Vreugdenhil 0 (1998) The role of gibberellin, abscisic acid, and sucrose in the regulation of potato tuber formation in vitro. Plant Physiol 117: 575-584 Yamaguchi S, Smith MW, Brown RGS, Kamiya Y, Sun TP (1998) Phytochrome regulation and differential expression of gibberellin 3f3-hydroxylase genes in germinating Arabidopsis seeds. Plant Cell 10: 2115-2126 " name="eprints.referencetext" /> <meta content="Reid, James B. and Botwright, Natasha A. and Smith, Jennifer J. and O'Neill, Damian P. and Kerckhoffs, L. Huub J. (2002) Control of Gibberellin Levels and Gene Expression during De-Etiolation in Pea. Plant Physiology, 128 (2). pp. 734-741. ISSN 0032-0889" name="eprints.citation" /> <meta content="http://eprints.utas.edu.au/2369/1/Control_of_Gibberellin.pdf" name="eprints.document_url" /> <link rel="schema.DC" href="http://purl.org/DC/elements/1.0/" /> <meta content="Control of Gibberellin Levels and Gene Expression during De-Etiolation in Pea" name="DC.title" /> <meta content="Reid, James B." name="DC.creator" /> <meta content="Botwright, Natasha A." name="DC.creator" /> <meta content="Smith, Jennifer J." name="DC.creator" /> <meta content="O'Neill, Damian P." name="DC.creator" /> <meta content="Kerckhoffs, L. Huub J." name="DC.creator" /> <meta content="320000 Medical and Health Sciences" name="DC.subject" /> <meta content="Gibberellin Al (GAl) levels drop significantly in wild-type pea (Pisum sativum) plants within 4 h of exposure to red, blue, or far-red light. This response is controlled by phytochrome A (phyA) (and not phyB) and a blue light receptor. GAs levels are increased in response to 4 h of red light, whereas the levels of GA19, GA2o, and GA29 do not vary substantially. Red light appears to control GAl levels by down-regulating the expression of Mendel's LE (PsGA30xl) gene that controls the conversion of GA20 to GAl' and by up-regulating PsGA2ox2, which codes for a GA 2-oxidase that converts GAl to GAs. This occurs within 0.5 to 1 h of exposure to red light. Similar responses occur in blue light. The major GA 20-oxidase gene expressed in shoots, PsGA20oxl, does not show substantial light regulation, but does show up-regulation after 4 h of red light, probably as a result of feedback regulation. Expression of PsGA30xl shows a similar feedback response, whereas PsGA2ox2 shows a feed-forward response. These results add to our understanding of how light reduces shoot elongation during de-etiolation." name="DC.description" /> <meta content="2002" name="DC.date" /> <meta content="Article" name="DC.type" /> <meta content="PeerReviewed" name="DC.type" /> <meta content="application/pdf" name="DC.format" /> <meta content="http://eprints.utas.edu.au/2369/1/Control_of_Gibberellin.pdf" name="DC.identifier" /> <meta content="http://proquest.umi.com/pqdweb?did=109212662&sid=1&Fmt=4&clientId=20931&RQT=309&VName=PQD" name="DC.relation" /> <meta content="Reid, James B. and Botwright, Natasha A. and Smith, Jennifer J. and O'Neill, Damian P. and Kerckhoffs, L. Huub J. (2002) Control of Gibberellin Levels and Gene Expression during De-Etiolation in Pea. Plant Physiology, 128 (2). pp. 734-741. 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border: solid 1px #ccc; padding: 3px"><tr> <td align="left"><a href="http://eprints.utas.edu.au/cgi/users/home">Login</a> | <a href="http://eprints.utas.edu.au/cgi/register">Create Account</a></td> <td align="right" style="white-space: nowrap"> <form method="get" accept-charset="utf-8" action="http://eprints.utas.edu.au/cgi/search" style="display:inline"> <input class="ep_tm_searchbarbox" size="20" type="text" name="q" /> <input class="ep_tm_searchbarbutton" value="Search" type="submit" name="_action_search" /> <input type="hidden" name="_order" value="bytitle" /> <input type="hidden" name="basic_srchtype" value="ALL" /> <input type="hidden" name="_satisfyall" value="ALL" /> </form> </td> </tr></table></td></tr> <tr> <td class="toplinks"><!-- InstanceBeginEditable name="content" --> <div align="center"> <table width="720" class="ep_tm_main"><tr><td align="left"> <h1 class="ep_tm_pagetitle">Control of Gibberellin Levels and Gene Expression during De-Etiolation in Pea</h1> <p style="margin-bottom: 1em" class="not_ep_block"><span class="person_name">Reid, James B.</span> and <span class="person_name">Botwright, Natasha A.</span> and <span class="person_name">Smith, Jennifer J.</span> and <span class="person_name">O'Neill, Damian P.</span> and <span class="person_name">Kerckhoffs, L. Huub J.</span> (2002) <xhtml:em>Control of Gibberellin Levels and Gene Expression during De-Etiolation in Pea.</xhtml:em> Plant Physiology, 128 (2). pp. 734-741. ISSN 0032-0889</p><p style="margin-bottom: 1em" class="not_ep_block"></p><table style="margin-bottom: 1em" class="not_ep_block"><tr><td valign="top" style="text-align:center"><a href="http://eprints.utas.edu.au/2369/1/Control_of_Gibberellin.pdf"><img alt="[img]" src="http://eprints.utas.edu.au/style/images/fileicons/application_pdf.png" class="ep_doc_icon" border="0" /></a></td><td valign="top"><a href="http://eprints.utas.edu.au/2369/1/Control_of_Gibberellin.pdf"><span class="ep_document_citation">PDF</span></a> - Full text restricted - Requires a PDF viewer<br />2840Kb</td><td><form method="get" accept-charset="utf-8" action="http://eprints.utas.edu.au/cgi/request_doc"><input accept-charset="utf-8" value="3053" name="docid" type="hidden" /><div class=""><input value="Request a copy" name="_action_null" class="ep_form_action_button" onclick="return EPJS_button_pushed( '_action_null' )" type="submit" /> </div></form></td></tr></table><p style="margin-bottom: 1em" class="not_ep_block">Official URL: <a href="http://proquest.umi.com/pqdweb?did=109212662&sid=1&Fmt=4&clientId=20931&RQT=309&VName=PQD">http://proquest.umi.com/pqdweb?did=109212662&sid=1&Fmt=4&clientId=20931&RQT=309&VName=PQD</a></p><div class="not_ep_block"><h2>Abstract</h2><p style="padding-bottom: 16px; text-align: left; margin: 1em auto 0em auto">Gibberellin Al (GAl) levels drop significantly in wild-type pea (Pisum sativum) plants within 4 h of exposure to red, blue, or far-red light. This response is controlled by phytochrome A (phyA) (and not phyB) and a blue light receptor. GAs levels are increased in response to 4 h of red light, whereas the levels of GA19, GA2o, and GA29 do not vary substantially. Red light appears to control GAl levels by down-regulating the expression of Mendel's LE (PsGA30xl) gene that controls the conversion of GA20 to GAl' and by up-regulating PsGA2ox2, which codes for a GA 2-oxidase that converts GAl to GAs. This occurs within 0.5 to 1 h of exposure to red light. Similar responses occur in blue light. The major GA 20-oxidase gene expressed in shoots, PsGA20oxl, does not show substantial light regulation, but does show up-regulation after 4 h of red light, probably as a result of feedback regulation. Expression of PsGA30xl shows a similar feedback response, whereas PsGA2ox2 shows a feed-forward response. These results add to our understanding of how light reduces shoot elongation during de-etiolation.</p></div><table style="margin-bottom: 1em" cellpadding="3" class="not_ep_block" border="0"><tr><th valign="top" class="ep_row">Item Type:</th><td valign="top" class="ep_row">Article</td></tr><tr><th valign="top" class="ep_row">Subjects:</th><td valign="top" class="ep_row"><a href="http://eprints.utas.edu.au/view/subjects/320000.html">320000 Medical and Health Sciences</a></td></tr><tr><th valign="top" class="ep_row">ID Code:</th><td valign="top" class="ep_row">2369</td></tr><tr><th valign="top" class="ep_row">Deposited By:</th><td valign="top" class="ep_row"><span class="ep_name_citation"><span class="person_name">Scholarly Publications Librarian</span></span></td></tr><tr><th valign="top" class="ep_row">Deposited On:</th><td valign="top" class="ep_row">31 Oct 2007 08:51</td></tr><tr><th valign="top" class="ep_row">Last Modified:</th><td valign="top" class="ep_row">09 Jan 2008 02:30</td></tr><tr><th valign="top" class="ep_row">ePrint Statistics:</th><td valign="top" class="ep_row"><a target="ePrintStats" href="/es/index.php?action=show_detail_eprint;id=2369;">View statistics for this ePrint</a></td></tr></table><p align="right">Repository Staff Only: <a href="http://eprints.utas.edu.au/cgi/users/home?screen=EPrint::View&eprintid=2369">item control page</a></p> </td></tr></table> </div> <!-- InstanceEndEditable --></td> </tr> <tr> <td><!-- #BeginLibraryItem "/Library/footer_eprints.lbi" --> <table width="795" border="0" align="left" cellpadding="0" class="footer"> <tr valign="top"> <td colspan="2"><div align="center"><a href="http://www.utas.edu.au">UTAS home</a> | <a href="http://www.utas.edu.au/library/">Library home</a> | <a href="/">ePrints home</a> | <a href="/contact.html">contact</a> | <a href="/information.html">about</a> | <a href="/view/">browse</a> | <a href="/perl/search/simple">search</a> | <a href="/perl/register">register</a> | <a href="/perl/users/home">user area</a> | <a href="/help/">help</a></div><br /></td> </tr> <tr><td colspan="2"><p><img src="/images/eprints/footerline.gif" width="100%" height="4" /></p></td></tr> <tr valign="top"> <td width="68%" class="footer">Authorised by the University Librarian<br /> © University of Tasmania ABN 30 764 374 782<br /> <a href="http://www.utas.edu.au/cricos/">CRICOS Provider Code 00586B</a> | <a href="http://www.utas.edu.au/copyright/copyright_disclaimers.html">Copyright & Disclaimers</a> | <a href="http://www.utas.edu.au/accessibility/index.html">Accessibility</a> | <a href="http://eprints.utas.edu.au/feedback/">Site Feedback</a> </td> <td width="32%"><div align="right"> <p align="right" class="NoPrint"><a href="http://www.utas.edu.au/"><img src="http://www.utas.edu.au/shared/logos/unioftasstrip.gif" alt="University of Tasmania Home Page" width="260" height="16" border="0" align="right" /></a></p> <p align="right" class="NoPrint"><a href="http://www.utas.edu.au/"><br /> </a></p> </div></td> </tr> <tr valign="top"> <td><p> </p></td> <td><div align="right"><span class="NoPrint"><a href="http://www.eprints.org/software/"><img src="/images/eprintslogo.gif" alt="ePrints logo" width="77" height="29" border="0" align="bottom" /></a></span></div></td> </tr> </table> <!-- #EndLibraryItem --> <div align="center"></div></td> </tr> </table> </body> </html>