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- <meta content="Botwright, Tina L." name="eprints.creators_name" />
- <meta content="Rebetzke, G.J." name="eprints.creators_name" />
- <meta content="Condon, A.G." name="eprints.creators_name" />
- <meta content="Richards, R.A." name="eprints.creators_name" />
- <meta content="Tina.Acuna@utas.edu.au" name="eprints.creators_id" />
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- <meta content="Influence of the gibberellin-sensitive Rht8 dwarfing gene on leaf epidermal cell dimensions and early vigour in wheat (Triticum aestivum L.)" name="eprints.title" />
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- <meta content="Wheat, Triticum aestivum L., gibberellic acid, leaf epidermal cells, doubled-haploids, early vigour, leaf area,
- alternative dwarfing genes." name="eprints.keywords" />
- <meta content="Background and Aims The gibberellin-insensitive Rht-B1b and Rht-D1b dwarfing genes are known to reduce the
- size of cells in culms, leaves and coleoptiles of wheat. Resulting leaf area development of gibberellin-insensitive
- wheats is poor compared to standard height (Rht-B1a and Rht-D1a) genotypes. Alternative dwarfing genes to
- Rht-B1b and Rht-D1b are available that reduce plant height, such as the gibberellin-responsive Rht8 gene. This study
- aims to investigate if Rht8 has a similar dwarfing effect on the size of leaf cells to reduce leaf area.
- Methods The effect of Rht8 on cell size and leaf area was assessed in four types of epidermal cells (interstomatal,
- long, sister and bulliform) measured on leaf 2 of standard height (rht8) and semi-dwarf (Rht8) doubled-haploid lines
- (DHLs). The DHLs were derived from a cross between very vigorous, standard height (rht8) (‘Vigour18’) and less
- vigorous, semi-dwarf (Rht8) (’Chuan-Mai 180) parents.
- Key Results Large differences were observed in seedling vigour between the parents, where ‘Vigour18’ had a much
- greater plant leaf area than ‘Chuan-Mai 18’. Accordingly, ‘Vigour18’ had on average longer, wider and more
- epidermal cells and cell files than ‘Chuan-Mai 18’. Although there was correspondingly large genotypic variation
- among DHLs for these traits, the contrast between semi-dwarf Rht8 and tall rht8 DHLs revealed no difference in the
- size of leaf 2 or average cell characteristics. Hence, these traits were independent of plant height and therefore Rht8
- in the DHLs. Correlations for leaf and average cell size across DHLs revealed a strong and positive relationship
- between leaf width and cell files, while the relationships between leaf and cell width, and leaf and cell length were
- not statistically different. The relative contribution of the four cell types (long, sister, interstomatal and bulliform)
- to leaf size in the parents, comparative controls and DHLs is discussed.
- Conclusions Despite a large range in early vigour among the DHLs, none of the DHLs attained the leaf area or
- epidermal cell sizeandnumbersof the vigorous rht8 parent. Nonetheless, the potential exists to increase the early vigour
- of semi-dwarf wheats by using GA-sensitive dwarfing genes such as Rht8." name="eprints.abstract" />
- <meta content="2005" name="eprints.date" />
- <meta content="published" name="eprints.date_type" />
- <meta content="Annals of Botany" name="eprints.publication" />
- <meta content="95" name="eprints.volume" />
- <meta content="4" name="eprints.number" />
- <meta content="631-639" name="eprints.pagerange" />
- <meta content="10.1093/aob/mci069" name="eprints.id_number" />
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- <meta content="http://dx.doi.org/10.1093/aob/mci069" name="eprints.official_url" />
- <meta content="Allan RE. 1989. Agronomic comparisons between Rht1 and Rht2 semidwarf
- genes in winter wheat. Crop Science 29: 1103–1108.
- Allan RE, Vogel O, Burleigh J, Peterson C. 1961. Inheritance of
- coleoptile length and its association with culm length in four winter
- wheat crosses. Crop Science 1: 328–332.
- Beemster GTS, Masle J. 1996. Effects of soil resistance to root penetration
- on leaf expansion in wheat (Triticum aestivum L.): composition,
- number and size of epidermal cells in mature blades. Journal of
- Experimental Botany 47: 1651–1662.
- Botwright T, Condon AG, Rebetzke GJ, Richards RA. 2002. Field
- evaluation of early vigour for genetic improvement of grain yield in
- wheat. Australian Journal of Agricultural Research 53: 1137–1145.
- Botwright T, Rebetzke G, Condon T, Richards R. 2001. The effect of rht
- genotype and temperature on coleoptile growth and dry matter partitioning
- in young wheat seedlings. Australian Journal of Plant
- Physiology 28: 417–423.
- Condon AG, Richards RA, Rebetzke GJ, Farquhar GD. 2002. Improving
- intrinsic water-use efficiency and crop yield. Crop Science 42:
- 122–131.
- Ellis MH, Rebetzke G, Chandler P, Bonnett D, Spielmeyer W,
- Richards R. 2004. The effect of different height reducing genes on
- early growth of wheat. Functional Plant Biology 31: 583–589.
- Gale MD, Youssefian S. 1985. Dwarfing genes in wheat. In: Russell GE, ed.
- Progress in plant breeding – 1. London: Butterworths, 1–35.
- Hoogendoorn J, Rickson JM, Gale MD. 1990. Differences in leaf and
- stem anatomy related to plant height of tall and dwarf wheat
- (Triticum aestivum L.). Journal of Plant Physiology 136: 72–77.
- Keyes G, Sorrells ME, Setter TL. 1990. Gibberellic acid regulates cell
- wall extensibility in wheat (Triticum aestivum L.). Plant Physiology
- 92: 242–245.
- Keyes GJ, Paolillo DJ Jr, Sorrells ME. 1989. The effects of dwarfing
- genes Rht1 and Rht2 on cellular dimensions and rate of leaf
- elongation in wheat. Annals of Botany 64: 683–690.
- Lo´pez-Casta~neda C, Richards RA, Farquhar GD, Williamson RE.
- 1996. Seed and seedling characteristics contributing to variation in
- early vigour among temperate cereals. Crop Science 36: 1257–1266.
- Moore C, Rebetzke G, Richards R, Marshall D. 2001. Genetic variation
- for embryo size and its influence on early vigour in wheat. In:
- Eastwood R, ed. Proceedings of the 10th Australian Wheat Breeders
- Assembly, Mildura, Horsham: Victorian Department of Agriculture
- 177–180.
- Rebetzke G, Botwright T, Moore C, Richards R, Condon AG. 2004.
- Genotypic variation in specific leaf area for genetic improvement of
- early vigour in wheat. Field Crops Research 88: 179–189.
- Rebetzke GJ, Richards RA. 1999. Genetic improvement of early vigour in
- wheat. Australian Journal of Agricultural Research 50: 291–301.
- Rebetzke GJ, Richards RA. 2000. Gibberellic acid-sensitive dwarfing
- genes reduce plant height to increase kernel number and grain
- yield of wheat. Australian Journal of Agricultural Research 51:
- 235–245.
- Rebetzke GJ, Richards RA, Fischer VM, Mickelson BJ. 1999. Breeding
- long coleoptile, reduced height wheats. Euphytica 106: 159–168.
- Richards R, Lucaks Z. 2002. Seedling vigour in wheat–sources of variation
- for genetic and agronomic improvement. Australian Journal of
- Agricultural Research 53: 41–50.
- Richards RA, Townley-Smith TF. 1987. Variation in leaf area
- development and its effect on water use, yield and harvest index of
- droughted wheat. Australian Journal of Agricultural Research 38:
- 983–992.
- SAS. 1990. SAS/STAT user’s guide, Version 6, Ed 4 Vol 1. Cary, NC: SAS
- Institute Inc.
- Tonkinson CL, Lyndon RF, Arnold GM, Lenton JR. 1995. Effect of the
- Rht3 dwarfing gene on dynamics of cell extension in wheat leaves, and
- its modification by gibberellic acid and paclobutrazol. Journal of
- Experimental Botany 46: 1085–1092.
- Wenzel CL, Chandler PM, Cunningham RB, Passioura JB. 1997a.
- Characterization of the leaf epidermis of barley (Hordeum vulgare
- L. ‘Himalaya’). Annals of Botany 79: 41–46.
- Wenzel CL, Chandler PM, Cunningham RB, Passioura JB. 1997b.
- Comparative leaf epidermal anatomy of mutants of barley (Hordeum
- vulgare L. ‘Himalaya’) which differ in leaf length. Annals of Botany
- 79: 47–52" name="eprints.referencetext" />
- <meta content="Botwright, Tina L. and Rebetzke, G.J. and Condon, A.G. and Richards, R.A. (2005) Influence of the gibberellin-sensitive Rht8 dwarfing gene on leaf epidermal cell dimensions and early vigour in wheat (Triticum aestivum L.). Annals of Botany, 95 (4). pp. 631-639. ISSN 0305-7364" name="eprints.citation" />
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- <meta content="Botwright, Tina L." name="DC.creator" />
- <meta content="Rebetzke, G.J." name="DC.creator" />
- <meta content="Condon, A.G." name="DC.creator" />
- <meta content="Richards, R.A." name="DC.creator" />
- <meta content="300203 Plant Improvement (Selection, Breeding and Genetic Engineering)" name="DC.subject" />
- <meta content="Background and Aims The gibberellin-insensitive Rht-B1b and Rht-D1b dwarfing genes are known to reduce the
- size of cells in culms, leaves and coleoptiles of wheat. Resulting leaf area development of gibberellin-insensitive
- wheats is poor compared to standard height (Rht-B1a and Rht-D1a) genotypes. Alternative dwarfing genes to
- Rht-B1b and Rht-D1b are available that reduce plant height, such as the gibberellin-responsive Rht8 gene. This study
- aims to investigate if Rht8 has a similar dwarfing effect on the size of leaf cells to reduce leaf area.
- Methods The effect of Rht8 on cell size and leaf area was assessed in four types of epidermal cells (interstomatal,
- long, sister and bulliform) measured on leaf 2 of standard height (rht8) and semi-dwarf (Rht8) doubled-haploid lines
- (DHLs). The DHLs were derived from a cross between very vigorous, standard height (rht8) (‘Vigour18’) and less
- vigorous, semi-dwarf (Rht8) (’Chuan-Mai 180) parents.
- Key Results Large differences were observed in seedling vigour between the parents, where ‘Vigour18’ had a much
- greater plant leaf area than ‘Chuan-Mai 18’. Accordingly, ‘Vigour18’ had on average longer, wider and more
- epidermal cells and cell files than ‘Chuan-Mai 18’. Although there was correspondingly large genotypic variation
- among DHLs for these traits, the contrast between semi-dwarf Rht8 and tall rht8 DHLs revealed no difference in the
- size of leaf 2 or average cell characteristics. Hence, these traits were independent of plant height and therefore Rht8
- in the DHLs. Correlations for leaf and average cell size across DHLs revealed a strong and positive relationship
- between leaf width and cell files, while the relationships between leaf and cell width, and leaf and cell length were
- not statistically different. The relative contribution of the four cell types (long, sister, interstomatal and bulliform)
- to leaf size in the parents, comparative controls and DHLs is discussed.
- Conclusions Despite a large range in early vigour among the DHLs, none of the DHLs attained the leaf area or
- epidermal cell sizeandnumbersof the vigorous rht8 parent. Nonetheless, the potential exists to increase the early vigour
- of semi-dwarf wheats by using GA-sensitive dwarfing genes such as Rht8." name="DC.description" />
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- <h1 class="ep_tm_pagetitle">Influence of the gibberellin-sensitive Rht8 dwarfing gene on leaf epidermal cell dimensions and early vigour in wheat (Triticum aestivum L.)</h1>
- <p style="margin-bottom: 1em" class="not_ep_block"><span class="person_name">Botwright, Tina L.</span> and <span class="person_name">Rebetzke, G.J.</span> and <span class="person_name">Condon, A.G.</span> and <span class="person_name">Richards, R.A.</span> (2005) <xhtml:em>Influence of the gibberellin-sensitive Rht8 dwarfing gene on leaf epidermal cell dimensions and early vigour in wheat (Triticum aestivum L.).</xhtml:em> Annals of Botany, 95 (4). pp. 631-639. ISSN 0305-7364</p><p style="margin-bottom: 1em" class="not_ep_block"></p><table style="margin-bottom: 1em" class="not_ep_block"><tr><td valign="top" style="text-align:center"><a href="http://eprints.utas.edu.au/2487/1/Botwright_Ann_Bot.pdf"><img alt="[img]" src="http://eprints.utas.edu.au/style/images/fileicons/application_pdf.png" class="ep_doc_icon" border="0" /></a></td><td valign="top"><a href="http://eprints.utas.edu.au/2487/1/Botwright_Ann_Bot.pdf"><span class="ep_document_citation">PDF</span></a> - Full text restricted - Requires a PDF viewer<br />284Kb</td><td><form method="get" accept-charset="utf-8" action="http://eprints.utas.edu.au/cgi/request_doc"><input accept-charset="utf-8" value="3267" name="docid" type="hidden" /><div class=""><input value="Request a copy" name="_action_null" class="ep_form_action_button" onclick="return EPJS_button_pushed( '_action_null' )" type="submit" /> </div></form></td></tr></table><p style="margin-bottom: 1em" class="not_ep_block">Official URL: <a href="http://dx.doi.org/10.1093/aob/mci069">http://dx.doi.org/10.1093/aob/mci069</a></p><div class="not_ep_block"><h2>Abstract</h2><p style="padding-bottom: 16px; text-align: left; margin: 1em auto 0em auto">Background and Aims The gibberellin-insensitive Rht-B1b and Rht-D1b dwarfing genes are known to reduce the
- size of cells in culms, leaves and coleoptiles of wheat. Resulting leaf area development of gibberellin-insensitive
- wheats is poor compared to standard height (Rht-B1a and Rht-D1a) genotypes. Alternative dwarfing genes to
- Rht-B1b and Rht-D1b are available that reduce plant height, such as the gibberellin-responsive Rht8 gene. This study
- aims to investigate if Rht8 has a similar dwarfing effect on the size of leaf cells to reduce leaf area.
- Methods The effect of Rht8 on cell size and leaf area was assessed in four types of epidermal cells (interstomatal,
- long, sister and bulliform) measured on leaf 2 of standard height (rht8) and semi-dwarf (Rht8) doubled-haploid lines
- (DHLs). The DHLs were derived from a cross between very vigorous, standard height (rht8) (‘Vigour18’) and less
- vigorous, semi-dwarf (Rht8) (’Chuan-Mai 180) parents.
- Key Results Large differences were observed in seedling vigour between the parents, where ‘Vigour18’ had a much
- greater plant leaf area than ‘Chuan-Mai 18’. Accordingly, ‘Vigour18’ had on average longer, wider and more
- epidermal cells and cell files than ‘Chuan-Mai 18’. Although there was correspondingly large genotypic variation
- among DHLs for these traits, the contrast between semi-dwarf Rht8 and tall rht8 DHLs revealed no difference in the
- size of leaf 2 or average cell characteristics. Hence, these traits were independent of plant height and therefore Rht8
- in the DHLs. Correlations for leaf and average cell size across DHLs revealed a strong and positive relationship
- between leaf width and cell files, while the relationships between leaf and cell width, and leaf and cell length were
- not statistically different. The relative contribution of the four cell types (long, sister, interstomatal and bulliform)
- to leaf size in the parents, comparative controls and DHLs is discussed.
- Conclusions Despite a large range in early vigour among the DHLs, none of the DHLs attained the leaf area or
- epidermal cell sizeandnumbersof the vigorous rht8 parent. Nonetheless, the potential exists to increase the early vigour
- of semi-dwarf wheats by using GA-sensitive dwarfing genes such as Rht8.</p></div><table style="margin-bottom: 1em" cellpadding="3" class="not_ep_block" border="0"><tr><th valign="top" class="ep_row">Item Type:</th><td valign="top" class="ep_row">Article</td></tr><tr><th valign="top" class="ep_row">Keywords:</th><td valign="top" class="ep_row">Wheat, Triticum aestivum L., gibberellic acid, leaf epidermal cells, doubled-haploids, early vigour, leaf area,
- alternative dwarfing genes.</td></tr><tr><th valign="top" class="ep_row">Subjects:</th><td valign="top" class="ep_row"><a href="http://eprints.utas.edu.au/view/subjects/300203.html">300000 Agricultural, Veterinary and Environmental Sciences > 300200 Crop and Pasture Production > 300203 Plant Improvement (Selection, Breeding and Genetic Engineering)</a></td></tr><tr><th valign="top" class="ep_row">ID Code:</th><td valign="top" class="ep_row">2487</td></tr><tr><th valign="top" class="ep_row">Deposited By:</th><td valign="top" class="ep_row"><span class="ep_name_citation"><span class="person_name">Dr Tina LB Acuna</span></span></td></tr><tr><th valign="top" class="ep_row">Deposited On:</th><td valign="top" class="ep_row">16 Nov 2007 14:40</td></tr><tr><th valign="top" class="ep_row">Last Modified:</th><td valign="top" class="ep_row">09 Jan 2008 02:30</td></tr><tr><th valign="top" class="ep_row">ePrint Statistics:</th><td valign="top" class="ep_row"><a target="ePrintStats" href="/es/index.php?action=show_detail_eprint;id=2487;">View statistics for this ePrint</a></td></tr></table><p align="right">Repository Staff Only: <a href="http://eprints.utas.edu.au/cgi/users/home?screen=EPrint::View&eprintid=2487">item control page</a></p>
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