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<title>UTas ePrints - Effect of monounsaturated fat in the diet on the serum carotenoid levels</title>
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<meta content="Ahuja, K.D." name="eprints.creators_name" />
<meta content="kiran.ahuja@utas.edu.au" name="eprints.creators_id" />
<meta content="thesis" name="eprints.type" />
<meta content="2007-04-12" name="eprints.datestamp" />
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<meta content="Effect of monounsaturated fat in the diet on the serum carotenoid levels" name="eprints.title" />
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<meta content="lycopene, carotenoids, coronary heart disease, antioxidants, monounsaturated fat rich diet, high carbohydrate low fat diet, tomatoes" name="eprints.keywords" />
<meta content="Epidemiological data suggest that populations with higher serum/ tissue levels of
carotenoids have a lower risk of coronary heart disease (CHD), possibly due to
the antioxidant capacity. Lycopene, a carotenoid mainly found in tomatoes, has
been suggested to have the greatest antioxidant capacity of the carotenoids found
in fruits and vegetables. Carotenoids are fat-soluble compounds and their
absorption from the diet into the body may depend on the amount of dietary fat
ingested.
For years there has been debate about what energy source should replace the
saturated fat in the diet, to give the optimum serum lipid profile to reduce CHD
risk. Studies have compared monounsaturated fat rich diets with high
carbohydrate, low fat diets and have found that both diets decrease serum
cholesterol and low-density lipoprotein (LDL) cholesterol levels. Results for
high-density lipoprotein (HDL) cholesterol and triglycerides have been
inconsistent. However, it is of interest to study the effects of different diets on
lipid oxidation, as this may also influence CHD risk.
Studies have investigated the effect of different amounts of total fat on the serum
levels of carotenoids especially beta-carotene and lutein, but to our knowledge no
study has looked at the effect of different amounts of fats on the serum lycopene
levels, and whether this could subsequently affect the oxidation of LDL in vitro.
XIV
Two separate randomised crossover dietary intervention studies were conducted;
one in healthy men and the other in healthy women aged 20 to 70 years. The aim
was to compare the effects of monounsaturated fat rich (MUFA) diet (38% of
energy from fat) and high carbohydrate low fat (HCLF) diet (15% energy from
fat) with controlled lycopene content, on serum lycopene levels. Main sources of
lycopene in the diets were canned tomatoes and tomato soup for the study in
women (lycopene content -15.9 mg/day) and tomato paste and tomato soup for
the study in men (lycopene content - 20.2 mg/day). Serum lipids and lipoproteins
levels and in vitro oxidation of LDL particles were also measured.
Compared to the baseline levels there was a significant increase in the serum
trans lycopene and total lycopene levels after MUFA diet for the study in women.
Comparing the levels at the end of the two diets no difference was observed. In
the study in men serum trans, cis and total lycopene levels increased after the
MUFA and HCLF diet periods. There was no significant difference in trans; cis
and total lycopene levels at the end of two diets.
Thus, high levels of monounsaturated fat in the diet do not appear to increase
lycopene absorption and serum levels compared to very low fat diet. There was
however a better serum lipid profile after MUFA diet compared to HCLF diet.
Lag phase for the in vitro oxidation of LDL particles was also longer on the
MUFA diet. The lack of difference in antioxidant levels would indicate that this
was due to the different fatty acid component of the diet." name="eprints.abstract" />
<meta content="2001" name="eprints.date" />
<meta content="published" name="eprints.date_type" />
<meta content="163" name="eprints.pages" />
<meta content="University of Tasmania" name="eprints.institution" />
<meta content="School of Human Life Sciences" name="eprints.department" />
<meta content="rmaster" name="eprints.thesis_type" />
<meta content="(1995). Fats and oils: The facts. Meadowlea Foods, Australia.
Abbey, M., Belling, G. B., Noakes, M., Hirata, F., and Nestel, P. J. (1993a).
Oxidation of low-density lipoproteins: intraindividual variability and the
effect of dietary linoleate supplementation. Am J Clin Nutr 57, 391-8.
Abbey, M., Nestel, P. J., and Baghurst, P. A. (1993b). Antioxidant vitamins and
low-density-lipoprotein oxidation. Am J Clin Nutr 58, 525-32.
Agarwal, S., and Rao, A. V. (1998). Tomato lycopene and low density
lipoprotein oxidation: a human dietary intervention study. Lipids 33, 981-
4.
Agarwal, S., and Rao, A. V. (2000). Carotenoids and chronic diseases. Drug
Metabol Drug Interact 17, 189-210.
Albanes, D., Virtamo, J., Taylor, P. R., Rautalahti, M., Pietinen, P., and
Heinonen, O. P. (1997). Effects of supplemental beta-carotene, cigarette
smoking, and alcohol consumption on serum carotenoids in the Alpha-
Tocopherol, Beta-Carotene Cancer Prevention Study. Am J Clin Nutr 66,
366-72.
Ashton, E. L. (2000). Effects of dietary constituents on coronary heart disease
risk factors. PhD Thesis. In "School of Biological and Chemical
Sciences", pp. 1-275. Deakin University, Melbourne.
ATBC. (1994). The effect of vitamin E and beta carotene on the incidence of lung
cancer and other cancers in male smokers. The Alpha-Tocopherol, Beta
Carotene Cancer Prevention Study Group. N Engl J Med 330, 1029-35.
Austin, M. A., Hokanson, J. E., and Edwards, K. L. (1998). Hypertriglyceridemia
as a cardiovascular risk factor. Am J Cardiol 81, 7B-12B.
136
Australian Facts. (1999). Heart, Stroke & Vascular Disease. "Heart, Stroke &
Vascular Disease." Australian Institute of Health & Welfare, Australia.
Benfari, R. C. (1981). The multiple risk factor intervention trial (MRFIT). III.
The model for intervention. Prev Med 10, 426-42.
Berry, E. M., Eisenberg, S., Friedlander, Y., Harats, D., Kaufmann, N. A.,
Norman, Y., and Stein, Y. (1992). Effects of diets rich in
monounsaturated fatty acids on plasma lipoproteins--the Jerusalem
Nutrition Study. II. Monounsaturated fatty acids vs carbohydrates. Am J
Clin Nutr 56, 394-403.
Boaz, M., Smetana, S., Weinstein, T., Matas, Z., Gafter, U., Iaina, A., Knecht, A.,
Weissgarten, Y., Brunner, D., Fainaru, M., and Green, M. S. (2000).
Secondary prevention with antioxidants of cardiovascular disease in
endstage renal disease (SPACE): randomised placebo-controlled trial.
Lancet 356, 1213-8.
Bolton-Smith, C., Casey, C. E., Gey, K. F., Smith, W. C., and Tunstall-Pedoe, H.
(1991). Antioxidant vitamin intakes assessed using a food-frequency
questionnaire: correlation with biochemical status in smokers and nonsmokers.
Br J Nutr 65, 337-46.
Bonanome, A., Pagnan, A., Biffanti, S., Opportuno, A., Sorgato, F., Dorella, M.,
Maiorino, M., and Ursini, F. (1992). Effect of dietary monounsaturated
and polyunsaturated fatty acids on the susceptibility of plasma low density
lipoproteins to oxidative modification. Arterioscler Thromb 12, 529-33.
Bonifacj, C., Gerber, M., Scali, J., and Daures, J. P. (1997). Comparison of
dietary assessment methods in a southern French population: use of
137
weighed records, estimated-diet records and a food-frequency
questionnaire. Eur J Clin Nutr 51, 217-31.
Bowen, P. E., Garg, V., Stacewicz Sapuntzakis, M., Yelton, L., and Schreiner, R.
S. (1993). Variability of serum carotenoids in response to controlled diets
containing six servings of fruits and vegetables per day. Ann N Y Acad Sci
691, 241-3.
Brady, W. E., Mares Perlman, J. A., Bowen, P., and Stacewicz Sapuntzakis, M.
(1996). Human serum carotenoid concentrations are related to physiologic
and lifestyle factors. J Nutr 126, 129-37.
Britton, G. (1995). Structure and properties of carotenoids in relation to function.
FASEB J 9, 1551-8.
Brown, E. D., Micozzi, M. S., Craft, N. E., Bieri, J. G., Beecher, G., Edwards, B.
K., Rose, A., Taylor, P. R., and Smith, J. C., Jr. (1989). Plasma
carotenoids in normal men after a single ingestion of vegetables or
purified beta-carotene. Am J Clin Nutr 49, 1258-65.
Brown, L., Rosner, B., Willett, W. W., and Sacks, F. M. (1999). Cholesterollowering
effects of dietary fiber: a meta-analysis. Am J Clin Nutr 69, 30-
42.
Brown, W. V., Ginsberg, H., and Karmally, W. (1984). Diet and the decrease of
coronary heart disease. Am J Cardiol 54, 27c-29c.
Carroll, Y. L., Corridan, B. M., and Morrissey, P. A. (1999). Carotenoids in
young and elderly healthy humans: dietary intakes, biochemical status and
diet-plasma relationships. Eur J Clin Nutr 53, 644-53.
138
Casso, D., White, E., Patterson, R. E., Agurs-Collins, T., Kooperberg, C., and
Haines, P. S. (2000). Correlates of serum lycopene in older women.
Nutrition & Cancer 36, 163-9.
Castelli, W. P. (1984). Epidemiology of coronary heart disease: the Framingham
study. Am J Med 76, 4-12.
Castelli, W. P. (1986). The triglyceride issue: a view from Framingham. Am
Heart J 112, 432-7.
Castelli, W. P., Abbott, R. D., and McNamara, P. M. (1983). Summary estimates
of cholesterol used to predict coronary heart disease. Circulation 67, 730-
4.
Castelli, W. P., Garrison, R. J., Wilson, P. W., Abbott, R. D., Kalousdian, S., and
Kannel, W. B. (1986). Incidence of coronary heart disease and lipoprotein
cholesterol levels. The Framingham Study. JAMA 256, 2835-8.
Castenmiller, J. J., and West, C. E. (1998). Bioavailability and bioconversion of
carotenoids. Annu Rev Nutr 18, 19-38.
Castenmiller, J. J., West, C. E., Linssen, J. P., van het Hof, K. H., and Voragen,
A. G. (1999). The food matrix of spinach is a limiting factor in
determining the bioavailability of beta-carotene and to a lesser extent of
lutein in humans. J Nutr 129, 349-55.
Chopra, M., O'Neill, M. E., Keogh, N., Wortley, G., Southon, S., and Thurnham,
D. I. (2000). Influence of increased fruit and vegetable intake on plasma
and lipoprotein carotenoids and LDL oxidation in smokers and
nonsmokers. Clin Chem 46, 1818-29.
Chug Ahuja, J. K., Holden, J. M., Forman, M. R., Mangels, A. R., Beecher, G.
R., and Lanza, E. (1993). The development and application of a
139
carotenoid database for fruits, vegetables, and selected multicomponent
foods. J Am Diet Assoc 93, 318-23.
Clifton, P. M., and Abbey, M. (1997). Genetic control of response to dietary fat
and cholesterol. World Rev Nutr Diet 80, 1-14.
Clifton, P. M., and Nestel, P. J. (1992). Influence of gender, body mass index,
and age on response of plasma lipids to dietary fat plus cholesterol.
Arterioscler Thromb 12, 955-62.
Colditz, G. A., Manson, J. E., Stampfer, M. J., Rosner, B., Willett, W. C., and
Speizer, F. E. (1992). Diet and risk of clinical diabetes in women. Am J
Clin Nutr 55, 1018-23.
Cole, T. G., Bowen, P. E., Schmeisser, D., Prewitt, T. E., Aye, P., Langenberg,
P., Dolecek, T. A., Brace, L. D., and Kamath, S. (1992). Differential
reduction of plasma cholesterol by the American Heart Association Phase
3 Diet in moderately hypercholesterolemic, premenopausal women with
different body mass indexes. Am J Clin Nutr 55, 385-94.
Colquhoun, D. M., Moores, D., Somerset, S. M., and Humphries, J. A. (1992).
Comparison of the effects on lipoproteins and apolipoproteins of a diet
high in monounsaturated fatty acids, enriched with avocado, and a highcarbohydrate
diet. Am J Clin Nutr 56, 671-7.
Colquhoun, D. M., Somerset, S., Glasziou, P., Richards, D., and Weyers, J.
(1998). Comparison of an Olive oil enriched diet to a Low fat diet
Intervention study using Vascular Endpoints - assessed by repeat
quantitative angiography (OLIVE study). Australian Journal of Nutrition
and Dietetics 55, S24-S29.
140
Dayton, S., and Pearce, M. L. (1969). Prevention of coronary heart disease and
other complications of arteriosclerosis by modified diet. Am J Med 46,
751-62.
de Lorgeril, M., Renaud, S., Mamelle, N., Salen, P., Martin, J. L., Monjaud, I.,
Guidollet, J., Touboul, P., and Delaye, J. (1994). Mediterranean alphalinolenic
acid-rich diet in secondary prevention of coronary heart disease.
Lancet 343, 1454-9.
de Lorgeril, M., Salen, P., Martin, J. L., Monjaud, I., Delaye, J., and Mamelle, N.
(1999). Mediterranean diet, traditional risk factors, and the rate of
cardiovascular complications after myocardial infarction: final report of
the Lyon Diet Heart Study. Circulation 99, 779-85.
Di Mascio, P., Devasagayam, T. P., Kaiser, S., and Sies, H. (1990). Carotenoids,
tocopherols and thiols as biological singlet molecular oxygen quenchers.
Biochem Soc Trans 18, 1054-6.
Di Mascio, P., Kaiser, S., and Sies, H. (1989). Lycopene as the most efficient
biological carotenoid singlet oxygen quencher. Arch Biochem Biophys
274, 532-8.
Dimitrov, N. V., Meyer, C., Ullrey, D. E., Chenoweth, W., Michelakis, A.,
Malone, W., Boone, C., and Fink, G. (1988). Bioavailability of betacarotene
in humans. Am J Clin Nutr 48, 298-304.
Document, W. (1988). Measuring obesity: Classification and Description of
Anthropometric Data. WHO regional office for Europe, Nutrition Unit,
Copenhagen.
D'Odorico, A., Martines, D., Kiechl, S., Egger, G., Oberhollenzer, F., Bonvicini,
P., Sturniolo, G. C., Naccarato, R., and Willeit, J. (2000). High plasma
141
levels of alpha- and beta-carotene are associated with a lower risk of
atherosclerosis: results from the Bruneck study. Atherosclerosis 153, 231-
9.
Dugas, T. R., Morel, D. W., and Harrison, E. H. (1999). Dietary supplementation
with beta-carotene, but not with lycopene, inhibits endothelial cellmediated
oxidation of low-density lipoprotein. Free Radic Biol Med 26,
1238-44.
Elinder, L. S., Hadell, K., Johansson, J., Holme, J. M., Olsson, A. G., and
Walldius, G. (1995). Probucol treatment decreases serum concentration of
diet-derived antioxidants. Arterioscler Thromb Vasc Biol 15, 1057-631.
Erdman, J. W., Jr., Bierer, T. L., and Gugger, E. T. (1993). Absorption and
transport of carotenoids. Ann N Y Acad Sci 691, 76-85.
Esrey, K. L., Joseph, L., and Grover, S. A. (1996). Relationship between dietary
intake and coronary heart disease mortality: lipid research clinics
prevalence follow-up study. J Clin Epidemiol 49, 211-6.
Esterbauer, H., Gebicki, J., Puhl, H., and Jurgens, G. (1992). The role of lipid
peroxidation and antioxidants in oxidative modification of LDL. Free
Radic Biol Med 13, 341-90.
Esterbauer, H., Striegl, G., Puhl, H., Oberreither, S., Rotheneder, M., el-Saadani,
M., and Jurgens, G. (1989). The role of vitamin E and carotenoids in
preventing oxidation of low density lipoproteins. Ann N Y Acad Sci 570,
254-67.
Evans, R. W., Shaten, B. J., Day, B. W., and Kuller, L. H. (1998). Prospective
association between lipid soluble antioxidants and coronary heart disease
142
in men. The Multiple Risk Factor Intervention Trial. Am J Epidemiol 147,
180-6.
Fliess, J. (1986). The design and Analysis of Clinical Experiments. "The design
and Analysis of Clinical Experiments." New York.
Forman, M. R., Beecher, G. R., Lanza, E., Reichman, M. E., Graubard, B. I.,
Campbell, W. S., Marr, T., Yong, L. C., Judd, J. T., and Taylor, P. R.
(1995). Effect of alcohol consumption on plasma carotenoid
concentrations in premenopausal women: a controlled dietary study. Am J
Clin Nutr 62, 131-5.
Forman, M. R., Beecher, G. R., Muesing, R., Lanza, E., Olson, B., Campbell, W.
S., McAdam, P., Raymond, E., Schulman, J. D., and Graubard, B. I.
(1996). The fluctuation of plasma carotenoid concentrations by phase of
the menstrual cycle: a controlled diet study. Am J Clin Nutr 64, 559-65.
Forman, M. R., Johnson, E. J., Lanza, E., Graubard, B. I., Beecher, G. R., and
Muesing, R. (1998). Effect of menstrual cycle phase on the concentration
of individual carotenoids in lipoproteins of premenopausal women: a
controlled dietary study. Am J Clin Nutr 67, 81-7.
Friedewald, W. T., Levy, R. I., and Fredrickson, D. S. (1972). Estimation of the
concentration of low-density lipoprotein cholesterol in plasma, without
use of the preparative ultracentrifuge. Clin Chem 18, 499-502.
Frost, G., Leeds, A. A., Dore, C. J., Madeiros, S., Brading, S., and Dornhorst, A.
(1999). Glycaemic index as a determinant of serum HDL-cholesterol
concentration. Lancet 353, 1045-8.
Fuhrman, B., Elis, A., and Aviram, M. (1997). Hypocholesterolemic effect of
lycopene and beta-carotene is related to suppression of cholesterol
143
synthesis and augmentation of LDL receptor activity in macrophages.
Biochemical and Biophysical Research Communications 233, 658-662.
Garcia-Palmieri, M. R., Sorlie, P., Tillotson, J., Costas, R., Jr., Cordero, E., and
Rodriguez, M. (1980). Relationship of dietary intake to subsequent
coronary heart disease incidence: The Puerto Rico Heart Health Program.
Am J Clin Nutr 33, 1818-27.
Gardner, C. D., and Kraemer, H. C. (1995). Monounsaturated versus
polyunsaturated dietary fat and serum lipids. A meta-analysis.
Arterioscler Thromb Vasc Biol 15, 1917-27.
Garg, A., Bonanome, A., Grundy, S. M., Zhang, Z. J., and Unger, R. H. (1988).
Comparison of a high-carbohydrate diet with a high-monounsaturated-fat
diet in patients with non-insulin-dependent diabetes mellitus. N Engl J
Med 319, 829-34.
Gartner, C., Stahl, W., and Sies, H. (1997). Lycopene is more bioavailable from
tomato paste than from fresh tomatoes. Am J Clin Nutr 66, 116-122.
Gaziano, J. M., Hatta, A., Flynn, M., Johnson, E. J., Krinsky, N. I., Ridker, P. M.,
Hennekens, C. H., and Frei, B. (1995a). Supplementation with betacarotene
in vivo and in vitro does not inhibit low density lipoprotein
oxidation. Atherosclerosis 112, 187-95.
Gaziano, J. M., and Manson, J. E. (1996). Diet and heart disease. The role of fat,
alcohol, and antioxidants. Cardiol Clin 14, 69-83.
Gaziano, J. M., Manson, J. E., Branch, L. G., Colditz, G. A., Willett, W. C., and
Buring, J. E. (1995b). A prospective study of consumption of carotenoids
in fruits and vegetables and decreased cardiovascular mortality in the
elderly. Ann Epidemiol 5, 255-60.
144
Gaziano, J. M., Manson, J. E., Buring, J. E., and Hennekens, C. H. (1992).
Dietary antioxidants and cardiovascular disease. Ann N Y Acad Sci 669,
249-58.
Gey, K. F., Puska, P., Jordan, P., and Moser, U. K. (1991). Inverse correlation
between plasma vitamin E and mortality from ischemic heart disease in
cross-cultural epidemiology. Am J Clin Nutr 53, 326s-334s.
Gey, K. F., Stahelin, H. B., and Eichholzer, M. (1993). Poor plasma status of
carotene and vitamin C is associated with higher mortality from ischemic
heart disease and stroke: Basel Prospective Study. Clin Investig 71, 3-6.
Giovannucci, E., and Clinton, S. K. (1998). Tomatoes, lycopene, and prostate
cancer. Proc Soc Exp Biol Med 218, 129-39.
GISSI. (1999). Dietary supplementation with n-3 polyunsaturated fatty acids and
vitamin E after myocardial infarction: results of the GISSI-Prevenzione
trial. Gruppo Italiano per lo Studio della Sopravvivenza nell'Infarto
miocardico. Lancet 354, 447-55.
Gordon, D. J., Probstfield, J. L., Garrison, R. J., Neaton, J. D., Castelli, W. P.,
Knoke, J. D., Jacobs, D. R., Bangdiwala, S., and Tyroler, H. A. (1989).
High-density lipoprotein cholesterol and cardiovascular disease. Four
prospective American studies. Circulation 79, 8-15.
Gordon, T., Kannel, W. B., Castelli, W. P., and Dawber, T. R. (1981).
Lipoproteins, cardiovascular disease, and death. The Framingham study.
Arch Intern Med 141, 1128-31.
Grundy, S. M. (1986). Comparison of monounsaturated fatty acids and
carbohydrates for lowering plasma cholesterol. N Engl J Med 314, 745-8.
145
Grundy, S. M., Florentin, L., Nix, D., and Whelan, M. F. (1988). Comparison of
monounsaturated fatty acids and carbohydrates for reducing raised levels
of plasma cholesterol in man. Am J Clin Nutr 47, 965-9.
Halliwell, B. (1994). Free Radicals and Antioxidants: A Personal View. Nutrition
Reviews 52, 253-265.
Handelman, G. J., Epstein, W. L., Peerson, J., Spiegelman, D., Machlin, L. J., and
Dratz, E. A. (1994). Human adipose alpha-tocopherol and gammatocopherol
kinetics during and after 1 y of alpha-tocopherol
supplementation. Am J Clin Nutr 59, 1025-32.
Handelman, G. J., Packer, L., and Cross, C. E. (1996). Destruction of
tocopherols, carotenoids, and retinol in human plasma by cigarette smoke.
Am J Clin Nutr 63, 559-65.
Heber, D. (1996). Plasma carotenoids and the menstrual cycle. Am J Clin Nutr
64, 640.
Hegsted, D. M., McGandy, R. B., Myers, M. L., and Stare, F. J. (1965).
Quantitative effects of dietary fat on serum cholesterol in man. Am J Clin
Nutr 17, 281-95.
Hennekens, C. H., Buring, J. E., Manson, J. E., Stampfer, M., Rosner, B., Cook,
N. R., Belanger, C., LaMotte, F., Gaziano, J. M., Ridker, P. M., Willett,
W., and Peto, R. (1996). Lack of effect of long-term supplementation with
beta carotene on the incidence of malignant neoplasms and cardiovascular
disease. N Engl J Med 334, 1145-9.
Hininger, I. A., Meyer-Wenger, A., Moser, U., Wright, A., Southon, S.,
Thurnham, D., Chopra, M., Van Den Berg, H., Olmedilla, B., Favier, A.
E., and Roussel, A. M. (2001). No significant effects of lutein, lycopene
146
or beta-carotene supplementation on biological markers of oxidative stress
and LDL oxidizability in healthy adult subjects. J Am Coll Nutr 20, 232-8.
Hjermann, I. (1983). A randomized primary preventive trial in coronary heart
disease: the Oslo study. Prev Med 12, 181-4.
Hodis, H. N., and Mack, W. J. (1995). Triglyceride-rich lipoproteins and the
progression of coronary artery disease. Curr Opin Lipidol 6, 209-14.
Hu, F. B., Stampfer, M. J., Rimm, E., Ascherio, A., Rosner, B. A., Spiegelman,
D., and Willett, W. C. (1999). Dietary fat and coronary heart disease: a
comparison of approaches for adjusting for total energy intake and
modeling repeated dietary measurements. Am J Epidemiol 149, 531-40.
Jalal, F., Nesheim, M. C., Agus, Z., Sanjur, D., and Habicht, J. P. (1998). Serum
retinol concentrations in children are affected by food sources of betacarotene,
fat intake, and anthelmintic drug treatment. Am J Clin Nutr 68,
623-9.
Jayarajan, P., Reddy, V., and Mohanram, M. (1980). Effect of dietary fat on
absorption of beta carotene from green leafy vegetables in children.
Indian Journal of Medical Research 71, 53-6.
Jenkins, D. J., Kendall, C. W., Vuksan, V., Augustin, L. S., Mehling, C., Parker,
T., Vidgen, E., Lee, B., Faulkner, D., Seyler, H., Josse, R., Leiter, L. A.,
Connelly, P. W., and Fulgoni, V., 3rd. (1999). Effect of wheat bran on
serum lipids: influence of particle size and wheat protein. J Am Coll Nutr
18, 159-65.
Jessup, W., Dean, R. T., de Whalley, C. V., Rankin, S. M., and Leake, D. S.
(1990). The role of oxidative modification and antioxidants in LDL
147
metabolism and atherosclerosis. Advances in Experimental Medicine &
Biology 264, 139-42.
Jialal, I., and Fuller, C. J. (1995). Effect of vitamin E, vitamin C and betacarotene
on LDL oxidation and atherosclerosis. Can J Cardiol 11 Suppl
G, 97G-103G.
Jialal, I., and Grundy, S. M. (1993). Effect of combined supplementation with
alpha-tocopherol, ascorbate, and beta carotene on low-density lipoprotein
oxidation. Circulation 88, 2780-6.
Jialal, I., Norkus, E. P., Cristol, L., and Grundy, S. M. (1991). beta-Carotene
inhibits the oxidative modification of low-density lipoprotein. Biochimica
et Biophysica Acta 1086, 134-8.
Johnson, E. J., Qin, J., Krinsky, N. I., and Russell, R. M. (1997). Ingestion by
men of a combined dose of beta-carotene and lycopene does not affect the
absorption of beta-carotene but improves that of lycopene. J Nutr 127,
1833-7.
Johnson, E. J., Qin, J., Krinsky, N. I., and Russell, R. M. (1998). Human studies
on bioavailability and plasma response of lycopene. Proc Soc Exp Biol
Med 218, 115-20.
Jula, A., Seppanen, R., and Alanen, E. (1999). Influence of days of the week on
the reported food, macronutrient and alcohol intake among an adult
population in south western Finland. Euro J Clin Nutr 52, 808-812.
Kardinaal, A. F., Kok, F. J., Ringstad, J., Gomez Aracena, J., Mazaev, V. P.,
Kohlmeier, L., Martin, B. C., Aro, A., Kark, J. D., Delgado Rodriguez,
M., and et al. (1993). Antioxidants in adipose tissue and risk of
myocardial infarction: the EURAMIC Study. Lancet 342, 1379-84.
148
Keys, A., Anderson, J. T., and Grande, F. (1957). Prediction of serum cholesterol
responses of man to changes in fats in the diet. Lancet 2, 959-966.
Keys, A., Anderson, J. T., and Grande, F. (1958). Effect of serum cholesterol in
man of moono-ene fatty acids (oleic acaid) in the diet. Proc Soc Exp Biol
Med 98, 387-91.
Keys, A., Menotti, A., Karvonen, M. J., Aravanis, C., Blackburn, H., Buzina, R.,
Djordjevic, B. S., Dontas, A. S., Fidanza, F., and Keys, M. H. (1986). The
diet and 15-year death rate in the seven countries study. Am J Epidemiol
124, 903-15.
Khachik, F., Beecher, G. R., Goli, M. B., Lusby, W. R., and Smith, J. C., Jr.
(1992). Separation and identification of carotenoids and their oxidation
products in the extracts of human plasma. Anal Chem 64, 2111-22.
Klipstein Grobusch, K., Geleijnse, J. M., den Breeijen, J. H., Boeing, H.,
Hofman, A., Grobbee, D. E., and Witteman, J. C. (1999). Dietary
antioxidants and risk of myocardial infarction in the elderly: the
Rotterdam Study. Am J Clin Nutr 69, 261-6.
Klipstein Grobusch, K., Launer, L. J., Geleijnse, J. M., Boeing, H., Hofman, A.,
and Witteman, J. C. (2000). Serum carotenoids and atherosclerosis. The
Rotterdam Study. Atherosclerosis 148, 49-56.
Knekt, P., Reunanen, A., Jarvinen, R., Seppanen, R., Heliovaara, M., and
Aromaa, A. (1994). Antioxidant vitamin intake and coronary mortality in
a longitudinal population study. Am J Epidemiol 139, 1180-9.
Kohlmeier, L., Kark, J. D., Gomez Gracia, E., Martin, B. C., Steck, S. E.,
Kardinaal, A. F., Ringstad, J., Thamm, M., Masaev, V., Riemersma, R.,
Martin Moreno, J. M., Huttunen, J. K., and Kok, F. J. (1997). Lycopene
149
and myocardial infarction risk in the EURAMIC Study. Am J Epidemiol
146, 618-26.
Kostic, D., White, W. S., and Olson, J. A. (1995). Intestinal absorption, serum
clearance, and interactions between lutein and beta-carotene when
administered to human adults in separate or combined oral doses. Am J
Clin Nutr 62, 604-10.
Krinsky, N. I. (1998a). The antioxidant and biological properties of the
carotenoids. Ann N Y Acad Sci 854, 443-7.
Krinsky, N. I. (1998b). Overview of lycopene, carotenoids, and disease
prevention. Proc Soc Exp Biol Med 218, 95-7.
Kristenson, M., Zieden, B., Kucinskiene, Z., Elinder, L. S., Bergdahl, B., Elwing,
B., Abaravicius, A., Razinkoviene, L., Calkauskas, H., and Olsson, A. G.
(1997). Antioxidant state and mortality from coronary heart disease in
Lithuanian and Swedish men: concomitant cross sectional study of men
aged 50. BMJ 314, 629-33.
Kromhout, D. (1989). Food consumption patterns in the Seven Countries Study.
Seven Countries Study Research Group. Annals of Medicine 21, 237-8.
Kromhout, D., Menotti, A., Bloemberg, B., Aravanis, C., Blackburn, H., and
Buzina, R. (1995). Dietary saturated and trans fatty acids and cholesterol
and 25-year mortality from coronary heart disease: the seven countries
study. Prev Med 24, 308-15.
Kushi, L. H., Folsom, A. R., Prineas, R. J., Mink, P. J., Wu, Y., and Bostick, R.
M. (1996). Dietary antioxidant vitamins and death from coronary heart
disease in postmenopausal women. N Engl J Med 334, 1156-62.
150
Kushi, L. H., Lenart, E. B., and Willett, W. C. (1995). Health implications of
Mediterranean diets in light of contemporary knowledge. 1. Plant foods
and dairy products. Am J Clin Nutr 61, 1407s-1415s.
Kushi, L. H., Lew, R. A., Stare, F. J., Ellison, C. R., el Lozy, M., Bourke, G.,
Daly, L., Graham, I., Hickey, N., Mulcahy, R., and et al. (1985). Diet and
20-year mortality from coronary heart disease. The Ireland-Boston Diet-
Heart Study. N Engl J Med 312, 811-8.
LaRosa, J. C., Hunninghake, D., Bush, D., Criqui, M. H., Getz, G. S., Gotto, A.
M., Grundy, S. M., Rakita, L., Robertson, R. M., Weisfeldt, M. L., and et
al. (1990). The cholesterol facts. A summary of the evidence relating
dietary fats, serum cholesterol, and coronary heart disease. A joint
statement by the American Heart Association and the National Heart,
Lung, and Blood Institute. The Task Force on Cholesterol Issues,
American Heart Association. Circulation 81, 1721-33.
Lavy, A., Ben Amotz, A., and Aviram, M. (1993). Preferential inhibition of LDL
oxidation by the all-trans isomer of beta-carotene in comparison with 9-
cis beta-carotene. Eur J Clin Chem Clin Biochem 31, 83-90.
Lee, A., Thurnham, D. I., and Chopra, M. (2000). Consumption of tomato
products with olive oil but not sunflower oil increases the antioxidant
activity of plasma. Free Rad Biol & Med 29, 1051-5.
Lessin, W., Catigani, G., and Schwartz, S. (1997). Quantification of cis-trans
isomers of provitamin A carotenoids in fresh fruits and vegetables. J
Agric Food Chem 45, 3728-3732.
Lowry, B. (1985). Protein Determination Method. Analytical Biochemistry 150,
76.
151
Luhrmann, P. M., Herbert, B. M., Gaster, C., and Neuhauser Berthold, M. (1999).
Validation of a self-administered 3-day estimated dietary record for use in
the elderly. Eur J Nutr 38, 235-40.
Mangels, A. R., Holden, J. M., Beecher, G. R., Forman, M. R., and Lanza, E.
(1993). Carotenoid content of fruits and vegetables: an evaluation of
analytic data. J Am Diet Assoc 93, 284-96.
Martini, M. C., Campbell, D. R., Gross, M. D., Grandits, G. A., Potter, J. D., and
Slavin, J. L. (1995). Plasma carotenoids as biomarkers of vegetable
intake: the University of Minnesota Cancer Prevention Research Unit
Feeding Studies. Cancer Epidemiol Biomarkers Prev 4, 491-6.
Mata, P., Alonso, R., Lopez Farre, A., Ordovas, J. M., Lahoz, C., Garces, C.,
Caramelo, C., Codoceo, R., Blazquez, E., and de Oya, M. (1996). Effect
of dietary fat saturation on LDL oxidation and monocyte adhesion to
human endothelial cells in vitro. Arterioscler Thromb Vasc Biol 16, 1347-
55.
Mata, P., Varela, O., Alonso, R., Lahoz, C., de Oya, M., and Badimon, L. (1997).
Monounsaturated and polyunsaturated n-6 fatty acid-enriched diets
modify LDL oxidation and decrease human coronary smooth muscle cell
DNA synthesis. Arterioscler Thromb Vasc Biol 17, 2088-95.
Mayne, S. T., Cartmel, B., Silva, F., Kim, C. S., Fallon, B. G., Briskin, K.,
Zheng, T., Baum, M., Shor Posner, G., and Goodwin, W. J., Jr. (1999).
Plasma lycopene concentrations in humans are determined by lycopene
intake, plasma cholesterol concentrations and selected demographic
factors. J Nutr 129, 849-54.
152
Mensink, R. P., de Groot, M. J., van den Broeke, L. T., Severijnen Nobels, A. P.,
Demacker, P. N., and Katan, M. B. (1989). Effects of monounsaturated
fatty acids v complex carbohydrates on serum lipoproteins and
apoproteins in healthy men and women. Metabolism 38, 172-8.
Mensink, R. P., and Katan, M. B. (1987). Effect of monounsaturated fatty acids
versus complex carbohydrates on high-density lipoproteins in healthy men
and women. Lancet 1, 122-5.
Mensink, R. P., and Katan, M. B. (1989). Effect of a diet enriched with
monounsaturated or polyunsaturated fatty acids on levels of low-density
and high-density lipoprotein cholesterol in healthy women and men. N
Engl J Med 321, 436-41.
Mensink, R. P., and Katan, M. B. (1992). Effect of dietary fatty acids on serum
lipids and lipoproteins. A meta-analysis of 27 trials. Arterioscler Thromb
12, 911-9.
Michaud, D. S., Feskanich, D., Rimm, E. B., Colditz, G. A., Speizer, F. E.,
Willett, W. C., and Giovannucci, E. (2000). Intake of specific carotenoids
and risk of lung cancer in 2 prospective US cohorts. Am J Clin Nutr 72,
990-7.
Micozzi, M. S., Brown, E. D., Edwards, B. K., Bieri, J. G., Taylor, P. R.,
Khachik, F., Beecher, G. R., and Smith, J. C., Jr. (1992). Plasma
carotenoid response to chronic intake of selected foods and beta-carotene
supplements in men. Am J Clin Nutr 55, 1120-5.
Morris, D. L., Kritchevsky, S. B., and Davis, C. E. (1994). Serum carotenoids and
coronary heart disease. The Lipid Research Clinics Coronary Primary
Prevention Trial and Follow-up Study. JAMA 272, 1439-41.
153
Mortensen, A., Skibsted, L. H., Sampson, J., Rice Evans, C., and Everett, S. A.
(1997). Comparative mechanisms and rates of free radical scavenging by
carotenoid antioxidants. FEBS Lett 418, 91-7.
Nguyen, M. L., and Schwartz, S. J. (1998). Lycopene stability during food
processing. Proc Soc Exp Biol Med 218, 101-5.
NHMRC. (1991). Recommended Dietary Intakes for use in Australia.
"Recommended Dietary Intakes for use in Australia." Australian
Goverenment Publishing Service, Canberra.
O'Bryne, D. J., O'Keefe, S. F., and Shireman, R. B. (1998). Low-fat,
monounsaturate-rich diets reduce susceptibility of low density
lipoproteins to peroxidation ex vivo. Lipids 33, 149-57.
Olmedilla, B., Granado, F., Blanco, I., and Rojas Hidalgo, E. (1994). Seasonal
and sex-related variations in six serum carotenoids, retinol, and alphatocopherol.
Am J Clin Nutr 60, 106-10.
Olmedilla, B., Granado, F., Southon, S., Wright, A. J., Blanco, I., Gil-Martinez,
E., Berg, H., Corridan, B., Roussel, A. M., Chopra, M., and Thurnham, D.
I. (2001). Serum concentrations of carotenoids and vitamins A, E, and C
in control subjects from five European countries. Br J Nutr 85, 227-38.
Olson, J. A. (1989). Biological actions of carotenoids. J Nutr 119, 94-5.
Omenn, G. S., Goodman, G. E., Thornquist, M. D., Balmes, J., Cullen, M. R.,
Glass, A., Keogh, J. P., Meyskens, F. L., Valanis, B., Williams, J. H.,
Barnhart, S., Cherniack, M. G., Brodkin, C. A., and Hammar, S. (1996).
Risk factors for lung cancer and for intervention effects in CARET, the
Beta-Carotene and Retinol Efficacy Trial. J Natl Cancer Inst 88, 1550-9.
154
Packer, L. (1993). Antioxidant action of carotenoids in vitro and in vivo and
protection against oxidation of human low-density lipoproteins. Ann N Y
Acad Sci 691, 48-60.
Pamuk, E. R., Byers, T., Coates, R. J., Vann, J. W., Sowell, A. L., Gunter, E. W.,
and Glass, D. (1994). Effect of smoking on serum nutrient concentrations
in African-American women. Am J Clin Nutr 59, 891-5.
Pandey, D. K., Shekelle, R., Selwyn, B. J., Tangney, C., and Stamler, J. (1995).
Dietary vitamin C and beta-carotene and risk of death in middle-aged
men. The Western Electric Study. Am J Epidemiol 142, 1269-78.
Parker, R. S. (1996). Absorption, metabolism, and transport of carotenoids.
FASEB J 10, 542-51.
Parker, R. S. (1997). Bioavailability of carotenoids. Eur J Clin Nutr 51 Suppl 1,
S86-90.
Parks, E. J., and Hellerstein, M. K. (2000). Carbohydrate-induced
hypertriacylglycerolemia: historical perspective and review of biological
mechanisms. Am J Clin Nutr 71, 412-33.
Parthasarathy, S., Auge, N., and Santanam, N. (1998). Implications of lag time
concept in the oxidation of LDL. Free Radic Res 28, 583-91.
Peng, Y. M., Peng, Y. S., Lin, Y., Moon, T., Roe, D. J., and Ritenbaugh, C.
(1995). Concentrations and plasma-tissue-diet relationships of
carotenoids, retinoids, and tocopherols in humans. Nutr Cancer 23, 233-
46.
Porrini, M., Riso, P., and Testolin, G. (1998). Absorption of lycopene from single
or daily portions of raw and processed tomato. Br J Nutr 80, 353-61.
155
Princen, H. M., van Poppel, G., Vogelezang, C., Buytenhek, R., and Kok, F. J.
(1992). Supplementation with vitamin E but not beta-carotene in vivo
protects low density lipoprotein from lipid peroxidation in vitro. Effect of
cigarette smoking. Arterioscler Thromb 12, 554-62.
Rao, A. V., and Agarwal, S. (1998). Effect of diet and smoking on serum
lycopene and lipid peroxidation. Nutrition Research 18, 713-721.
Rao, A. V., and Agarwal, S. (1999). Role of lycopene as antioxidant carotenoid in
the prevention of chronic diseases: a review. Nutrition Research 19, 305-
323.
Rao, A. V., Zeeshan, W., and Agarwal, S. (1998). Lycopene content of tomatoes
and tomato products and their contribution to dietary lycopene. Food
Research International 31, 737-741.
Rapola, J. M., Virtamo, J., Ripatti, S., Haukka, J. K., Huttunen, J. K., Albanes,
D., Taylor, P. R., and Heinonen, O. P. (1998). Effects of alpha tocopherol
and beta carotene supplements on symptoms, progression, and prognosis
of angina pectoris. Heart 79, 454-8.
Reaven, P., Parthasarathy, S., Grasse, B. J., Miller, E., Almazan, F., Mattson, F.
H., Khoo, J. C., Steinberg, D., and Witztum, J. L. (1991). Feasibility of
using an oleate-rich diet to reduce the susceptibility of low-density
lipoprotein to oxidative modification in humans. Am J Clin Nutr 54, 701-
6.
Reaven, P. D., Grasse, B. J., and Tribble, D. L. (1994). Effects of linoleateenriched
and oleate-enriched diets in combination with alpha-tocopherol
on the susceptibility of LDL and LDL subfractions to oxidative
modification in humans. Arterioscler Thromb 14, 557-66.
156
Reaven, P. D., Khouw, A., Beltz, W. F., Parthasarathy, S., and Witztum, J. L.
(1993). Effect of dietary antioxidant combinations in humans. Protection
of LDL by vitamin E but not by beta-carotene. Arterioscler Thromb 13,
590-600.
Retzlaff, B. M., Walden, C. E., Dowdy, A. A., McCann, B. S., Anderson, K. V.,
and Knopp, R. H. (1995). Changes in plasma triacylglycerol
concentrations among free-living hyperlipidemic men adopting different
carbohydrate intakes over 2 y: the Dietary Alternatives Study. Am J Clin
Nutr 62, 988-95.
Riemersma, R. A., Wood, D. A., Macintyre, C. C., Elton, R. A., Gey, K. F., and
Oliver, M. F. (1991). Risk of angina pectoris and plasma concentrations
of vitamins A, C, and E and carotene. Lancet 337, 1-5.
Rimm, E. B., Stampfer, M. J., Ascherio, A., Giovannucci, E., Colditz, G. A., and
Willett, W. C. (1993). Vitamin E consumption and the risk of coronary
heart disease in men. N Engl J Med 328, 1450-6.
Rissanen, T. H., Voutilainen, S., Nyyssonen, K., Lakka, T. A., Sivenius, J.,
Salonen, R., Kaplan, G. A., and Salonen, J. T. (2001). Low serum
lycopene concentration is associated with an excess incidence of acute
coronary events and stroke: the Kuopio Ischaemic Heart Disease Risk
Factor Study. Br J Nutr 85, 749-54.
Roberts, W. C. (1989). Atherosclerotic risk factors--are there ten or is there only
one? Am J Cardiol 64, 552-4.
Rock, C. L., Jacob, R. A., and Bowen, P. E. (1996). Update on the biological
characteristics of the antioxidant micronutrients: vitamin C, vitamin E,
and the carotenoids. J Am Diet Assoc 96, 693-702.
157
Rock, C. L., Lovalvo, J. L., Emenhiser, C., Ruffin, M. T., Flatt, S. W., and
Schwartz, S. J. (1998). Bioavailability of beta-carotene is lower in raw
than in processed carrots and spinach in women. J Nutr 128, 913-6.
Rock, C. L., and Swendseid, M. E. (1992). Plasma beta-carotene response in
humans after meals supplemented with dietary pectin. Am J Clin Nutr 55,
96-9.
Rock, C. L., Swendseid, M. E., Jacob, R. A., and McKee, R. W. (1992). Plasma
carotenoid levels in human subjects fed a low carotenoid diet. J Nutr 122,
96-100.
Roidt, L., White, E., Goodman, G. E., Wahl, P. W., Omenn, G. S., Rollins, B.,
and Karkeck, J. M. (1988). Association of food frequency questionnaire
estimates of vitamin A intake with serum vitamin A levels. Am J
Epidemiol 128, 645-54.
Roodenburg, A. J., Leenen, R., van het Hof, K. H., Weststrate, J. A., and Tijburg,
L. B. (2000). Amount of fat in the diet affects bioavailability of lutein
esters but not of alpha-carotene, beta-carotene, and vitamin E in humans.
Am J Clin Nutr 71, 1187-93.
Ross, M. A., Crosley, L. K., Brown, K. M., Duthie, S. J., Collins, A. C., Arthur, J.
R., and Duthie, G. G. (1995). Plasma concentrations of carotenoids and
antioxidant vitamins in Scottish males: influences of smoking. Eur J Clin
Nutr 49, 861-5.
Sahyoun, N. R., Jacques, P. F., and Russell, R. M. (1996). Carotenoids, vitamins
C and E, and mortality in an elderly population. Am J Epidemiol 144, 501-
11.
158
Sattler, W., Kostner, G. M., Waeg, G., and Esterbauer, H. (1991). Oxidation of
lipoprotein Lp(a). A comparison with low-density lipoproteins. Biochim
Biophys Acta 1081, 65-74.
Schaefer, E. J., Lichtenstein, A. H., Lamon-Fava, S., McNamara, J. R., Schaefer,
M. M., Rasmussen, H., and Ordovas, J. M. (1995). Body weight and lowdensity
lipoprotein cholesterol changes after consumption of a low-fat ad
libitum diet. JAMA 274, 1450-5.
Schierle, J., Bretzel, W., Buehler, I., Faccin, N., Hess, D., Steiner, K., and
Schueep, W. (1997). Content and isomeric ratio of lycopene in food and
human blood plasma. Food Chemistry 59, 459-465.
Schlagheck, T. G., Riccardi, K. A., Zorich, N. L., Torri, S. A., Dugan, L. D., and
Peters, J. C. (1997). Olestra dose response on fat-soluble and watersoluble
nutrients in humans. J Nutr 127, 1646s-1665s.
Schmitz, H. H., Poor, C. L., Wellman, R. B., and Erdman, J. W., Jr. (1991).
Concentrations of selected carotenoids and vitamin A in human liver,
kidney and lung tissue. J Nutr 121, 1613-21.
Scott, K. J., Thurnham, D. I., Hart, D. J., Bingham, S. A., and Day, K. (1996).
The correlation between the intake of lutein, lycopene and beta-carotene
from vegetables and fruits, and blood plasma concentrations in a group of
women aged 50-65 years in the UK. Br J Nutr 75, 409-18.
Sies, H., and Stahl, W. (1995). Vitamins E and C, beta-carotene, and other
carotenoids as antioxidants. Am J Clin Nutr 62, 1315s-1321s.
Sies, H., and Stahl, W. (1998). Lycopene: antioxidant and biological effects and
its bioavailability in the human. Proc Soc Exp Biol Med 218, 121-4.
159
Skeaff, M. (1998). Essentials of human nutrition. "Essentials of human nutrition."
Oxford University Press.
Stahl, W., and Sies, H. (1992). Uptake of lycopene and its geometrical isomers is
greater from heat-processed than from unprocessed tomato juice in
humans. J Nutr 122, 2161-6.
Stampfer, M. J., Hennekens, C. H., Manson, J. E., Colditz, G. A., Rosner, B., and
Willett, W. C. (1993). Vitamin E consumption and the risk of coronary
disease in women. N Engl J Med 328, 1444-9.
Stampfer, M. J., Sacks, F. M., Salvini, S., Willett, W. C., and Hennekens, C. H.
(1991). A prospective study of cholesterol, apolipoproteins, and the risk
of myocardial infarction. N Engl J Med 325, 373-81.
Starc, T. J., Shea, S., Cohn, L. C., Mosca, L., Gersony, W. M., and Deckelbaum,
R. J. (1998). Greater dietary intake of simple carbohydrate is associated
with lower concentrations of high-density-lipoprotein cholesterol in
hypercholesterolemic children. Am J Clin Nutr 67, 1147-54.
Steinberg, D., Parthasarathy, S., Carew, T. E., Khoo, J. C., and Witztum, J. L.
(1989). Beyond cholesterol. Modifications of low-density lipoprotein that
increase its atherogenicity. N Engl J Med 320, 915-24.
Steinberg, D., and Witztum, J. L. (1990). Lipoproteins and atherogenesis. Current
concepts. JAMA 264, 3047-52.
Stephens, N. G., Parsons, A., Schofield, P. M., Kelly, F., Cheeseman, K., and
Mitchinson, M. J. (1996). Randomised controlled trial of vitamin E in
patients with coronary disease: Cambridge Heart Antioxidant Study
(CHAOS). Lancet 347, 781-6.
160
Street, D. A., Comstock, G. W., Salkeld, R. M., Schuep, W., and Klag, M. J.
(1994). Serum antioxidants and myocardial infarction. Are low levels of
carotenoids and alpha-tocopherol risk factors for myocardial infarction?
Circulation 90, 1154-61.
Su, Q., Rowley, K. G., and O'Dea, K. (1999). Stability of individual carotenoids,
retinol and tocopherols in human plasma during exposure to light and
after extraction. J Chromatogr B Biomed Sci Appl 729, 191-8.
Sutherland, W. H., Walker, R. J., De Jong, S. A., and Upritchard, J. E. (1999).
Supplementation with tomato juice increases plasma lycopene but does
not alter susceptibility to oxidation of low-density lipoproteins from renal
transplant recipients. Clin Nephrol 52, 30-6.
Tinkler, J. H., Bohm, F., Schalch, W., and Truscott, T. G. (1994). Dietary
carotenoids protect human cells from damage. J Photochem Photobiol B
26, 283-5.
Turley, M. L., Skeaff, C. M., Mann, J. I., and Cox, B. (1998). The effect of a lowfat,
high-carbohydrate diet on serum high density lipoprotein cholesterol
and triglyceride. Eur J Clin Nutr 52, 728-32.
Turpeinen, O., Karvonen, M. J., Pekkarinen, M., Miettinen, M., Elosuo, R., and
Paavilainen, E. (1979). Dietary prevention of coronary heart disease: the
Finnish Mental Hospital Study. Int J Epidemiol 8, 99-118.
Upritchard, J. E., Sutherland, W. H., and Mann, J. I. (2000). Effect of
supplementation with tomato juice, vitamin E, and vitamin C on LDL
oxidation and products of inflammatory activity in type 2 diabetes.
Diabetes Care 23, 733-8.
161
USDA. (1998). USDA-NCC Carotenoid Database for U.S. Foods: National
Cancer Institute.
http://www.nal.usda.gov/fnic/foodcomp/Data/car98/car98.html.
van het Hof, K. H., Brouwer, I. A., West, C. E., Haddeman, E., Steegers-
Theunissen, R. P., van Dusseldorp, M., Weststrate, J. A., Eskes, T. K.,
and Hautvast, J. G. (1999a). Bioavailability of lutein from vegetables is 5
times higher than that of beta-carotene. Am J Clin Nutr 70, 261-8.
van het Hof, K. H., de Boer, B. C., Tijburg, L. B., Lucius, B. R., Zijp, I., West, C.
E., Hautvast, J. G., and Weststrate, J. A. (2000). Carotenoid
bioavailability in humans from tomatoes processed in different ways
determined from the carotenoid response in the triglyceride-rich
lipoprotein fraction of plasma after a single consumption and in plasma
after four days of consumption. J Nutr 130, 1189-96.
van het Hof, K. H., Tijburg, L. B., Pietrzik, K., and Weststrate, J. A. (1999b).
Influence of feeding different vegetables on plasma levels of carotenoids,
folate and vitamin C. Effect of disruption of the vegetable matrix. Br J
Nutr 82, 203-12.
van Het Hof, K. H., West, C. E., Weststrate, J. A., and Hautvast, J. G. (2000).
Dietary factors that affect the bioavailability of carotenoids. J Nutr 130,
503-6.
Verlangieri, A. J., Kapeghian, J. C., el-Dean, S., and Bush, M. (1985). Fruit and
vegetable consumption and cardiovascular mortality. Med Hypotheses 16,
7-15.
Wahlqvist, M. L., Wattanapenpaiboon, N., Macrae, F. A., Lambert, J. R.,
MacLennan, R., and Hsu Hage, B. H. (1994). Changes in serum
162
carotenoids in subjects with colorectal adenomas after 24 mo of betacarotene
supplementation. Australian Polyp Prevention Project
Investigators. Am J Clin Nutr 60, 936-43.
White, W. S., Peck, K. M., Bierer, T. L., Gugger, E. T., and Erdman, J. W., Jr.
(1993). Interactions of oral beta-carotene and canthaxanthin in ferrets. J
Nutr 123, 1405-13.
WHO. (1974). World Health Organization Collaborative Group: An
interventional controlled trial in the multifactorial prevention of coronary
heart disease. Int J Epidemiol 3, 219-224.
WHO. (1990). Diet, nutrition and the prevention of chronic diseases. WHO,
Geneva.
Wilhelmsen, L., Berglund, G., Elmfeldt, D., Tibblin, G., Wedel, H., Pennert, K.,
Vedin, A., Wilhelmsson, C., and Werko, L. (1986). The multifactor
primary prevention trial in Gotenborg, Sweden. Eur Heart J 7, 279-88.
Willett, W. C. (1994). Diet and health: what should we eat? Science 264, 532-7.
Williams, A. W., Boileau, T. W., and Erdman, J. W., Jr. (1998). Factors
influencing the uptake and absorption of carotenoids. Proc Soc Exp Biol
Med 218, 106-8.
Witztum, J. (1994). The oxidation hypothesis of atherosclerosis. Lancet 344, 793-
5.
Witztum, J., and Steinberg, D. (1991). Role of oxidized low density lipoprotein in
atherogenesis. J Clin Invest 14, 605-616.
Witztum, J. L. (1991). The role of oxidized LDL in atherosclerosis. Adv Exp Med
Biol 285, 353-65.
163
Yeum, K. J., Booth, S. L., Sadowski, J. A., Liu, C., Tang, G., Krinsky, N. I., and
Russell, R. M. (1996). Human plasma carotenoid response to the ingestion
of controlled diets high in fruits and vegetables. Am J Clin Nutr 64, 594-
602.
Yeum, K. J., Lee-Kim, Y. C., Zhu, S., Xiao, S., Mason, J., and Russell, R. M.
(1999). Serum concentration of teh antioxidant nutrients in healthy
American, Chinese and Korean adults. Asia Pacific J Clin Nutr 8, 4-8.
Yusuf, S., Dagenais, G., Pogue, J., Bosch, J., and Sleight, P. (2000). Vitamin E
supplementation and cardiovascular events in high-risk patients. The
Heart Outcomes Prevention Evaluation Study Investigators. N Engl J Med
342, 154-60." name="eprints.referencetext" />
<meta content="Ahuja, K.D. (2001) Effect of monounsaturated fat in the diet on the serum carotenoid levels. Research Master thesis, University of Tasmania." name="eprints.citation" />
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<meta content="Ahuja, K.D." name="DC.creator" />
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<meta content="Epidemiological data suggest that populations with higher serum/ tissue levels of
carotenoids have a lower risk of coronary heart disease (CHD), possibly due to
the antioxidant capacity. Lycopene, a carotenoid mainly found in tomatoes, has
been suggested to have the greatest antioxidant capacity of the carotenoids found
in fruits and vegetables. Carotenoids are fat-soluble compounds and their
absorption from the diet into the body may depend on the amount of dietary fat
ingested.
For years there has been debate about what energy source should replace the
saturated fat in the diet, to give the optimum serum lipid profile to reduce CHD
risk. Studies have compared monounsaturated fat rich diets with high
carbohydrate, low fat diets and have found that both diets decrease serum
cholesterol and low-density lipoprotein (LDL) cholesterol levels. Results for
high-density lipoprotein (HDL) cholesterol and triglycerides have been
inconsistent. However, it is of interest to study the effects of different diets on
lipid oxidation, as this may also influence CHD risk.
Studies have investigated the effect of different amounts of total fat on the serum
levels of carotenoids especially beta-carotene and lutein, but to our knowledge no
study has looked at the effect of different amounts of fats on the serum lycopene
levels, and whether this could subsequently affect the oxidation of LDL in vitro.
XIV
Two separate randomised crossover dietary intervention studies were conducted;
one in healthy men and the other in healthy women aged 20 to 70 years. The aim
was to compare the effects of monounsaturated fat rich (MUFA) diet (38% of
energy from fat) and high carbohydrate low fat (HCLF) diet (15% energy from
fat) with controlled lycopene content, on serum lycopene levels. Main sources of
lycopene in the diets were canned tomatoes and tomato soup for the study in
women (lycopene content -15.9 mg/day) and tomato paste and tomato soup for
the study in men (lycopene content - 20.2 mg/day). Serum lipids and lipoproteins
levels and in vitro oxidation of LDL particles were also measured.
Compared to the baseline levels there was a significant increase in the serum
trans lycopene and total lycopene levels after MUFA diet for the study in women.
Comparing the levels at the end of the two diets no difference was observed. In
the study in men serum trans, cis and total lycopene levels increased after the
MUFA and HCLF diet periods. There was no significant difference in trans; cis
and total lycopene levels at the end of two diets.
Thus, high levels of monounsaturated fat in the diet do not appear to increase
lycopene absorption and serum levels compared to very low fat diet. There was
however a better serum lipid profile after MUFA diet compared to HCLF diet.
Lag phase for the in vitro oxidation of LDL particles was also longer on the
MUFA diet. The lack of difference in antioxidant levels would indicate that this
was due to the different fatty acid component of the diet." name="DC.description" />
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<h1 class="ep_tm_pagetitle">Effect of monounsaturated fat in the diet on the serum carotenoid levels</h1>
<p style="margin-bottom: 1em" class="not_ep_block"><span class="person_name">Ahuja, K.D.</span> (2001) <xhtml:em>Effect of monounsaturated fat in the diet on the serum carotenoid levels.</xhtml:em> Research Master thesis, University of Tasmania.</p><p style="margin-bottom: 1em" class="not_ep_block"></p><table style="margin-bottom: 1em" class="not_ep_block"><tr><td valign="top" style="text-align:center"><a onmouseover="EPJS_ShowPreview( event, 'doc_preview_746' );" href="http://eprints.utas.edu.au/737/1/Front-Matter.pdf" onmouseout="EPJS_HidePreview( event, 'doc_preview_746' );"><img alt="[img]" src="http://eprints.utas.edu.au/style/images/fileicons/application_pdf.png" class="ep_doc_icon" border="0" /></a><div class="ep_preview" id="doc_preview_746"><table><tr><td><img alt="" src="http://eprints.utas.edu.au/737/thumbnails/1/preview.png" class="ep_preview_image" border="0" /><div class="ep_preview_title">Preview</div></td></tr></table></div></td><td valign="top"><a href="http://eprints.utas.edu.au/737/1/Front-Matter.pdf"><span class="ep_document_citation">PDF (Front Matter)</span></a> - Requires a PDF viewer<br />52Kb</td></tr><tr><td valign="top" style="text-align:center"><a onmouseover="EPJS_ShowPreview( event, 'doc_preview_747' );" href="http://eprints.utas.edu.au/737/2/Whole-Thesis.pdf" onmouseout="EPJS_HidePreview( event, 'doc_preview_747' );"><img alt="[img]" src="http://eprints.utas.edu.au/style/images/fileicons/application_pdf.png" class="ep_doc_icon" border="0" /></a><div class="ep_preview" id="doc_preview_747"><table><tr><td><img alt="" src="http://eprints.utas.edu.au/737/thumbnails/2/preview.png" class="ep_preview_image" border="0" /><div class="ep_preview_title">Preview</div></td></tr></table></div></td><td valign="top"><a href="http://eprints.utas.edu.au/737/2/Whole-Thesis.pdf"><span class="ep_document_citation">PDF (Whole Thesis)</span></a> - Requires a PDF viewer<br />1233Kb</td></tr></table><div class="not_ep_block"><h2>Abstract</h2><p style="padding-bottom: 16px; text-align: left; margin: 1em auto 0em auto">Epidemiological data suggest that populations with higher serum/ tissue levels of
carotenoids have a lower risk of coronary heart disease (CHD), possibly due to
the antioxidant capacity. Lycopene, a carotenoid mainly found in tomatoes, has
been suggested to have the greatest antioxidant capacity of the carotenoids found
in fruits and vegetables. Carotenoids are fat-soluble compounds and their
absorption from the diet into the body may depend on the amount of dietary fat
ingested.
For years there has been debate about what energy source should replace the
saturated fat in the diet, to give the optimum serum lipid profile to reduce CHD
risk. Studies have compared monounsaturated fat rich diets with high
carbohydrate, low fat diets and have found that both diets decrease serum
cholesterol and low-density lipoprotein (LDL) cholesterol levels. Results for
high-density lipoprotein (HDL) cholesterol and triglycerides have been
inconsistent. However, it is of interest to study the effects of different diets on
lipid oxidation, as this may also influence CHD risk.
Studies have investigated the effect of different amounts of total fat on the serum
levels of carotenoids especially beta-carotene and lutein, but to our knowledge no
study has looked at the effect of different amounts of fats on the serum lycopene
levels, and whether this could subsequently affect the oxidation of LDL in vitro.
XIV
Two separate randomised crossover dietary intervention studies were conducted;
one in healthy men and the other in healthy women aged 20 to 70 years. The aim
was to compare the effects of monounsaturated fat rich (MUFA) diet (38% of
energy from fat) and high carbohydrate low fat (HCLF) diet (15% energy from
fat) with controlled lycopene content, on serum lycopene levels. Main sources of
lycopene in the diets were canned tomatoes and tomato soup for the study in
women (lycopene content -15.9 mg/day) and tomato paste and tomato soup for
the study in men (lycopene content - 20.2 mg/day). Serum lipids and lipoproteins
levels and in vitro oxidation of LDL particles were also measured.
Compared to the baseline levels there was a significant increase in the serum
trans lycopene and total lycopene levels after MUFA diet for the study in women.
Comparing the levels at the end of the two diets no difference was observed. In
the study in men serum trans, cis and total lycopene levels increased after the
MUFA and HCLF diet periods. There was no significant difference in trans; cis
and total lycopene levels at the end of two diets.
Thus, high levels of monounsaturated fat in the diet do not appear to increase
lycopene absorption and serum levels compared to very low fat diet. There was
however a better serum lipid profile after MUFA diet compared to HCLF diet.
Lag phase for the in vitro oxidation of LDL particles was also longer on the
MUFA diet. The lack of difference in antioxidant levels would indicate that this
was due to the different fatty acid component of the diet.</p></div><table style="margin-bottom: 1em" cellpadding="3" class="not_ep_block" border="0"><tr><th valign="top" class="ep_row">Item Type:</th><td valign="top" class="ep_row">Thesis (Research Master)</td></tr><tr><th valign="top" class="ep_row">Keywords:</th><td valign="top" class="ep_row">lycopene, carotenoids, coronary heart disease, antioxidants, monounsaturated fat rich diet, high carbohydrate low fat diet, tomatoes</td></tr><tr><th valign="top" class="ep_row">Subjects:</th><td valign="top" class="ep_row"><a href="http://eprints.utas.edu.au/view/subjects/320000.html">320000 Medical and Health Sciences</a><br /><a href="http://eprints.utas.edu.au/view/subjects/321205.html">320000 Medical and Health Sciences > 321200 Public Health and Health Services > 321205 Nutrition and Dietetics</a></td></tr><tr><th valign="top" class="ep_row">ID Code:</th><td valign="top" class="ep_row">737</td></tr><tr><th valign="top" class="ep_row">Deposited By:</th><td valign="top" class="ep_row"><span class="ep_name_citation"><span class="person_name">Cherie Holmes</span></span></td></tr><tr><th valign="top" class="ep_row">Deposited On:</th><td valign="top" class="ep_row">12 Apr 2007</td></tr><tr><th valign="top" class="ep_row">Last Modified:</th><td valign="top" class="ep_row">09 Jan 2008 02:30</td></tr><tr><th valign="top" class="ep_row">ePrint Statistics:</th><td valign="top" class="ep_row"><a target="ePrintStats" href="/es/index.php?action=show_detail_eprint;id=737;">View statistics for this ePrint</a></td></tr></table><p align="right">Repository Staff Only: <a href="http://eprints.utas.edu.au/cgi/users/home?screen=EPrint::View&eprintid=737">item control page</a></p>
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