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- <meta content="Patel, Rahul P" name="eprints.creators_name" />
- <meta content="Narkowicz, Christian" name="eprints.creators_name" />
- <meta content="Hutchinson, Joseph" name="eprints.creators_name" />
- <meta content="Hilder, Emily" name="eprints.creators_name" />
- <meta content="Jacobson, Glenn" name="eprints.creators_name" />
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- <meta content="2007-12-13 04:50:44" name="eprints.datestamp" />
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- <meta content="Low molecular weight heparin; Dalteparin; Heparin; Capillary electrophoresis; Batch-to-batch variations" name="eprints.keywords" />
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- <meta content="A simple, selective and accurate capillary electrophoresis (CE) method has been developed for the rapid separation and identification of various
- low molecular weight heparins (LMWHs) and unfractionated heparin. Separation and operational parameters were investigated using dalteparin
- sodium as the test LMWH. The developed method used a 70 cm fused silica capillary (50 m i.d.) with a detection window 8.5 cm from the
- distal end. Phosphate electrolyte (pH 3.5; 50 mM), an applied voltage of −30 kV, UV detection at 230 nm and sample injection at 20 mbar for 5 s
- were used. The method performance was assessed in terms of linearity, selectivity, intra- and inter-day precision and accuracy. The method was
- successfully applied to the European Pharmacopeia LMWH standard, dalteparin sodium, enoxaparin sodium and heparin sodium with a significant
- reduction in the run time and increased resolution compared with previously reported CE methods. Different CE separation profiles were obtained
- for various LMWHs and unfractionated heparin showing significant structural diversity. The current methodology was sensitive enough to reveal
- minor constituent differences between two different batches of enoxaparin sodium. This CE method also clearly showed chemical changes that
- occurred to LMWHs under different stress conditions. The sensitivity, selectivity and simplicity of the developed method allow its application in
- research or manufacturing for the identification, stability analysis, characterization and monitoring of batch-to-batch consistency of different low
- molecular weight and unfractionated heparins.
- © 2007 Elsevier B.V. All rights reserved." name="eprints.abstract" />
- <meta content="2008" name="eprints.date" />
- <meta content="published" name="eprints.date_type" />
- <meta content="Journal of Pharmaceutical and Biomedical Analysis" name="eprints.publication" />
- <meta content="46" name="eprints.volume" />
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- [1] B. Casu, in: D.A. Lane, U. Lindahl (Eds.), Heparin: Chemical and Biological
- Properties. Clinical Applications, Edward Arnold, London, 1989, pp.
- 1–25.
- [2] M. Verstraete, Drugs 40 (1990) 498–530.
- [3] R.J. Linhardt, N.S. Gunay, Semin. Thromb. Hemost. 25 (1999) 5–16.
- [4] Fragmin (dalteparin sodium) injection (package insert), Pharmacia and
- Upjohn, Kalmazoo: March 2004.
- [5] J. Fareed, D. Hoppensteadt, C. Schultz, Q. Ma, M.F. Kujawski, B. Neville,
- H. Messmore, Curr. Pharm. Des. 10 (2004) 983–999.
- [6] British Pharmacopoeia Organisation. Low molecular weight heparins.
- In: British Pharmacopoeia. British Pharmacopoeia Commission, London
- 2002.
- [7] G.I. Rozenberg, J. Espada, L.L. De Cidre, A.M. Eijan, J.C. Calvo, G.E.
- Bertolesi, Electrophoresis 22 (2001) 3–11.
- [8] M.K. Cowman, M.F. Slahetka, D.M. Hittner, J. Kim, M. Forino, G.
- Gadelrab, Biochem. J. 221 (1984) 707–716.
- [9] R.E. Edens, A. Al-Hakim, J.M. Weiler, D.G. Rethwisch, J. Fareed, R.J.
- Linhardt, J. Pharm. Sci. 81 (1992) 823–827.
- [10] J.E. Turnbull, J.T. Gallagher, Biochem. J. 251 (1988) 597–608.
- [11] S. Cavari, S. Vannucchi, Clin. Chim. Acta 252 (1996) 159–170.
- [12] R.R. Vives, S. Goodger, D.A. Pye, Biochem. J. 354 (2001) 141–147.
- [13] W. Mao, C. Thanawirron, R. J. Linhardt, In: N.Volpi (Ed.), AnalyticalTechniques
- to Evaluate the Structure and Function of Natural Polysaccharides,
- Glycosaminoglycans. Research Signpost, Trivendrum, 2002, pp. 53–78.
- [14] C. Thanawiroon, R.J. Linhardt, J. Chromatogr. A 1014 (2003) 215–223.
- [15] J. Maddineni, J.M.Walenga,W.P. Jeske, D.A. Hoppensteadt, J. Fareed, R.
- Wahi, R.L. Bick, Clin. Appl. Thromb-Hem. 12 (2006) 267–276.
- [16] J.W. Jorgenson, TrAC. Trends Anal. Chem. 3 (1984) 51–54.
- [17] W.G. Kuhr, Anal. Chem. 62 (1990) 403R–414R.
- [18] S.A. Ampofo, H.M. Wang, R.J. Linhardt, Anal. Biochem. 199 (1991)
- 249–255.
- [19] J.B.L. Damm, G.T. Overklift, B.W.M. Vermeulen, C.F. Fluitsma, G.W.K.
- van Dedem, J. Chromatogr. 608 (1992) 297–309.
- [20] S.L. Carney, D.J. Osborne, Anal. Biochem. 195 (1991) 132–140.
- [21] A. Al-Hakim, R.J. Linhardt, Anal. Biochem. 195 (1991) 68–73.
- [22] U.R. Desai, H.M.Wang, S.A. Ampofo, R.J. Linhardt, Anal. Biochem. 213
- (1993) 120–127.
- [23] J. Grimshaw, Electrophoresis 18 (1997) 2408–2414.
- [24] N.K. Karamanos, A. Hjerpe, Biomed. Chromatogr. 13 (1999) 507–512.
- [25] R.J. Linhardt, A. Pervin, J. Chromatogr. A 720 (1996) 323–335.
- [26] A. Pervin, A. Al-Hakim, R.J. Linhardt, Anal. Biochem. 221 (1994)
- 182–188.
- [27] V. Ruiz-Calero, L. Puignou, M.T. Galceran, J. Chromatogr. A 828 (1998)
- 497–508.
- [28] T. Toida, R.J. Linhardt, Electrophoresis 17 (1996) 341–346.
- [29] R. Malsch, J. Harenberg, D.L. Heene, J. Chromatogr. A 716 (1995)
- 259–268.
- [30] R. Malsch, J. Harenberg, L. Piazolo, G. Huhle, D.L. Heene, J. Chromatogr.
- B 685 (1996) 223–231.
- [31] J. Grimshaw, A. Kane, J. Trocha-Grimshaw, A. Douglas, U. Chakravarthy,
- D. Archer, Electrophoresis 15 (1994) 936–940.
- [32] M.A. Roberts, H.J. Zhong, J. Prodolliet, D.M. Goodall, J. Chromatogr. A
- 817 (1998) 353–366.
- [33] S. Hayase, Y. Oda, S. Honda, K. Kakehi, J. Chromatogr. A 768 (1997)
- 295–305.
- [34] Z. Mala, L. Krivankova, P. Bocek, Electrophoresis 17 (1996) 125–129.
- [35] I. Ramasamy, J. Kennedy, K. Tan, Lab. Hematol. 9 (2003) 64–66.
- [36] M.D. Richmond, E.S. Yeung, Anal. Biochem. 210 (1993) 245–248.
- [37] J. Sudor, M. Novotny, P. Natl. Acad. Sci. U.S.A. 90 (1993) 9451–9455.
- [38] Z.E. Rassi, Y.A. Mechref, in: P. Camilleri (Ed.), Capillary Electrophoresis:
- Theory and Practice, CRC press, London, 1998, pp. 273–362.
- [39] K.A. Jandik, D. Kruep, M. Cartier, R.J. Linhardt, J. Pharm. Sci. 85 (1996)
- 45–51.
- [40] C.M. Mangin, D.M. Goodall, M.A. Roberts, Electrophoresis 22 (2001)
- 1460–1467." name="eprints.referencetext" />
- <meta content="Patel, Rahul P and Narkowicz, Christian and Hutchinson, Joseph and Hilder, Emily and Jacobson, Glenn (2008) A simple capillary electrophoresis method for the rapid separation and determination of intact low molecular weight and unfractionated heparins. Journal of Pharmaceutical and Biomedical Analysis, 46 (1). pp. 30-35. ISSN 0731-7085" name="eprints.citation" />
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- <meta content="A simple, selective and accurate capillary electrophoresis (CE) method has been developed for the rapid separation and identification of various
- low molecular weight heparins (LMWHs) and unfractionated heparin. Separation and operational parameters were investigated using dalteparin
- sodium as the test LMWH. The developed method used a 70 cm fused silica capillary (50 m i.d.) with a detection window 8.5 cm from the
- distal end. Phosphate electrolyte (pH 3.5; 50 mM), an applied voltage of −30 kV, UV detection at 230 nm and sample injection at 20 mbar for 5 s
- were used. The method performance was assessed in terms of linearity, selectivity, intra- and inter-day precision and accuracy. The method was
- successfully applied to the European Pharmacopeia LMWH standard, dalteparin sodium, enoxaparin sodium and heparin sodium with a significant
- reduction in the run time and increased resolution compared with previously reported CE methods. Different CE separation profiles were obtained
- for various LMWHs and unfractionated heparin showing significant structural diversity. The current methodology was sensitive enough to reveal
- minor constituent differences between two different batches of enoxaparin sodium. This CE method also clearly showed chemical changes that
- occurred to LMWHs under different stress conditions. The sensitivity, selectivity and simplicity of the developed method allow its application in
- research or manufacturing for the identification, stability analysis, characterization and monitoring of batch-to-batch consistency of different low
- molecular weight and unfractionated heparins.
- © 2007 Elsevier B.V. All rights reserved." name="DC.description" />
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- <h1 class="ep_tm_pagetitle">A simple capillary electrophoresis method for the rapid separation and determination of intact low molecular weight and unfractionated heparins</h1>
- <p style="margin-bottom: 1em" class="not_ep_block"><span class="person_name">Patel, Rahul P</span> and <span class="person_name">Narkowicz, Christian</span> and <span class="person_name">Hutchinson, Joseph</span> and <span class="person_name">Hilder, Emily</span> and <span class="person_name">Jacobson, Glenn</span> (2008) <xhtml:em>A simple capillary electrophoresis method for the rapid separation and determination of intact low molecular weight and unfractionated heparins.</xhtml:em> Journal of Pharmaceutical and Biomedical Analysis, 46 (1). pp. 30-35. ISSN 0731-7085</p><p style="margin-bottom: 1em" class="not_ep_block"></p><table style="margin-bottom: 1em" class="not_ep_block"><tr><td valign="top" style="text-align:center"><a href="http://eprints.utas.edu.au/2706/1/0030_-_0035_Patel.pdf"><img alt="[img]" src="http://eprints.utas.edu.au/style/images/fileicons/application_pdf.png" class="ep_doc_icon" border="0" /></a></td><td valign="top"><a href="http://eprints.utas.edu.au/2706/1/0030_-_0035_Patel.pdf"><span class="ep_document_citation">PDF</span></a> - Full text restricted until January 2008 - Requires a PDF viewer<br />340Kb</td><td><form method="get" accept-charset="utf-8" action="http://eprints.utas.edu.au/cgi/request_doc"><input accept-charset="utf-8" value="3582" name="docid" type="hidden" /><div class=""><input value="Request a copy" name="_action_null" class="ep_form_action_button" onclick="return EPJS_button_pushed( '_action_null' )" type="submit" /> </div></form></td></tr></table><p style="margin-bottom: 1em" class="not_ep_block">Official URL: <a href="http://dx.doi.org/10.1016/j.jpba.2007.10.009">http://dx.doi.org/10.1016/j.jpba.2007.10.009</a></p><div class="not_ep_block"><h2>Abstract</h2><p style="padding-bottom: 16px; text-align: left; margin: 1em auto 0em auto">A simple, selective and accurate capillary electrophoresis (CE) method has been developed for the rapid separation and identification of various
- low molecular weight heparins (LMWHs) and unfractionated heparin. Separation and operational parameters were investigated using dalteparin
- sodium as the test LMWH. The developed method used a 70 cm fused silica capillary (50 m i.d.) with a detection window 8.5 cm from the
- distal end. Phosphate electrolyte (pH 3.5; 50 mM), an applied voltage of −30 kV, UV detection at 230 nm and sample injection at 20 mbar for 5 s
- were used. The method performance was assessed in terms of linearity, selectivity, intra- and inter-day precision and accuracy. The method was
- successfully applied to the European Pharmacopeia LMWH standard, dalteparin sodium, enoxaparin sodium and heparin sodium with a significant
- reduction in the run time and increased resolution compared with previously reported CE methods. Different CE separation profiles were obtained
- for various LMWHs and unfractionated heparin showing significant structural diversity. The current methodology was sensitive enough to reveal
- minor constituent differences between two different batches of enoxaparin sodium. This CE method also clearly showed chemical changes that
- occurred to LMWHs under different stress conditions. The sensitivity, selectivity and simplicity of the developed method allow its application in
- research or manufacturing for the identification, stability analysis, characterization and monitoring of batch-to-batch consistency of different low
- molecular weight and unfractionated heparins.
- © 2007 Elsevier B.V. All rights reserved.</p></div><table style="margin-bottom: 1em" cellpadding="3" class="not_ep_block" border="0"><tr><th valign="top" class="ep_row">Item Type:</th><td valign="top" class="ep_row">Article</td></tr><tr><th valign="top" class="ep_row">Additional Information:</th><td valign="top" class="ep_row">The definitive version is available at http://www.sciencedirect.com
- </td></tr><tr><th valign="top" class="ep_row">Keywords:</th><td valign="top" class="ep_row">Low molecular weight heparin; Dalteparin; Heparin; Capillary electrophoresis; Batch-to-batch variations</td></tr><tr><th valign="top" class="ep_row">Subjects:</th><td valign="top" class="ep_row"><a href="http://eprints.utas.edu.au/view/subjects/320501.html">320000 Medical and Health Sciences > 320500 Pharmacology and Pharmaceutical Sciences > 320501 Pharmaceutical Sciences and Pharmacy</a></td></tr><tr><th valign="top" class="ep_row">ID Code:</th><td valign="top" class="ep_row">2706</td></tr><tr><th valign="top" class="ep_row">Deposited By:</th><td valign="top" class="ep_row"><span class="ep_name_citation"><span class="person_name">Mrs Gina Hadolt</span></span></td></tr><tr><th valign="top" class="ep_row">Deposited On:</th><td valign="top" class="ep_row">13 Dec 2007 15:50</td></tr><tr><th valign="top" class="ep_row">Last Modified:</th><td valign="top" class="ep_row">09 Jan 2008 02:30</td></tr><tr><th valign="top" class="ep_row">ePrint Statistics:</th><td valign="top" class="ep_row"><a target="ePrintStats" href="/es/index.php?action=show_detail_eprint;id=2706;">View statistics for this ePrint</a></td></tr></table><p align="right">Repository Staff Only: <a href="http://eprints.utas.edu.au/cgi/users/home?screen=EPrint::View&eprintid=2706">item control page</a></p>
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