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<meta content="Patel, Rahul P" name="eprints.creators_name" />
<meta content="Narkowicz, Christian" name="eprints.creators_name" />
<meta content="Hutchinson, Joseph" name="eprints.creators_name" />
<meta content="Hilder, Emily" name="eprints.creators_name" />
<meta content="Jacobson, Glenn" name="eprints.creators_name" />
<meta content="rppatel@utas.edu.au" name="eprints.creators_id" />
<meta content="c.narkowicz@utas.edu.a.u" name="eprints.creators_id" />
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<meta content="glenn.jacobson@utas.edu.au" name="eprints.creators_id" />
<meta content="article" name="eprints.type" />
<meta content="2007-12-13 04:50:44" name="eprints.datestamp" />
<meta content="2008-01-08 15:30:00" name="eprints.lastmod" />
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<meta content="A simple capillary electrophoresis method for the rapid separation and determination of intact low molecular weight and unfractionated heparins" name="eprints.title" />
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<meta content="Low molecular weight heparin; Dalteparin; Heparin; Capillary electrophoresis; Batch-to-batch variations" name="eprints.keywords" />
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<meta content="A simple, selective and accurate capillary electrophoresis (CE) method has been developed for the rapid separation and identification of various
low molecular weight heparins (LMWHs) and unfractionated heparin. Separation and operational parameters were investigated using dalteparin
sodium as the test LMWH. The developed method used a 70 cm fused silica capillary (50 m i.d.) with a detection window 8.5 cm from the
distal end. Phosphate electrolyte (pH 3.5; 50 mM), an applied voltage of −30 kV, UV detection at 230 nm and sample injection at 20 mbar for 5 s
were used. The method performance was assessed in terms of linearity, selectivity, intra- and inter-day precision and accuracy. The method was
successfully applied to the European Pharmacopeia LMWH standard, dalteparin sodium, enoxaparin sodium and heparin sodium with a significant
reduction in the run time and increased resolution compared with previously reported CE methods. Different CE separation profiles were obtained
for various LMWHs and unfractionated heparin showing significant structural diversity. The current methodology was sensitive enough to reveal
minor constituent differences between two different batches of enoxaparin sodium. This CE method also clearly showed chemical changes that
occurred to LMWHs under different stress conditions. The sensitivity, selectivity and simplicity of the developed method allow its application in
research or manufacturing for the identification, stability analysis, characterization and monitoring of batch-to-batch consistency of different low
molecular weight and unfractionated heparins.
© 2007 Elsevier B.V. All rights reserved." name="eprints.abstract" />
<meta content="2008" name="eprints.date" />
<meta content="published" name="eprints.date_type" />
<meta content="Journal of Pharmaceutical and Biomedical Analysis" name="eprints.publication" />
<meta content="46" name="eprints.volume" />
<meta content="1" name="eprints.number" />
<meta content="30-35" name="eprints.pagerange" />
<meta content="10.1016/j.jpba.2007.10.009" name="eprints.id_number" />
<meta content="TRUE" name="eprints.refereed" />
<meta content="0731-7085" name="eprints.issn" />
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1460–1467." name="eprints.referencetext" />
<meta content="Patel, Rahul P and Narkowicz, Christian and Hutchinson, Joseph and Hilder, Emily and Jacobson, Glenn (2008) A simple capillary electrophoresis method for the rapid separation and determination of intact low molecular weight and unfractionated heparins. Journal of Pharmaceutical and Biomedical Analysis, 46 (1). pp. 30-35. ISSN 0731-7085" name="eprints.citation" />
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<meta content="Patel, Rahul P" name="DC.creator" />
<meta content="Narkowicz, Christian" name="DC.creator" />
<meta content="Hutchinson, Joseph" name="DC.creator" />
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<meta content="Jacobson, Glenn" name="DC.creator" />
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<meta content="A simple, selective and accurate capillary electrophoresis (CE) method has been developed for the rapid separation and identification of various
low molecular weight heparins (LMWHs) and unfractionated heparin. Separation and operational parameters were investigated using dalteparin
sodium as the test LMWH. The developed method used a 70 cm fused silica capillary (50 m i.d.) with a detection window 8.5 cm from the
distal end. Phosphate electrolyte (pH 3.5; 50 mM), an applied voltage of −30 kV, UV detection at 230 nm and sample injection at 20 mbar for 5 s
were used. The method performance was assessed in terms of linearity, selectivity, intra- and inter-day precision and accuracy. The method was
successfully applied to the European Pharmacopeia LMWH standard, dalteparin sodium, enoxaparin sodium and heparin sodium with a significant
reduction in the run time and increased resolution compared with previously reported CE methods. Different CE separation profiles were obtained
for various LMWHs and unfractionated heparin showing significant structural diversity. The current methodology was sensitive enough to reveal
minor constituent differences between two different batches of enoxaparin sodium. This CE method also clearly showed chemical changes that
occurred to LMWHs under different stress conditions. The sensitivity, selectivity and simplicity of the developed method allow its application in
research or manufacturing for the identification, stability analysis, characterization and monitoring of batch-to-batch consistency of different low
molecular weight and unfractionated heparins.
© 2007 Elsevier B.V. All rights reserved." name="DC.description" />
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<h1 class="ep_tm_pagetitle">A simple capillary electrophoresis method for the rapid separation and determination of intact low molecular weight and unfractionated heparins</h1>
<p style="margin-bottom: 1em" class="not_ep_block"><span class="person_name">Patel, Rahul P</span> and <span class="person_name">Narkowicz, Christian</span> and <span class="person_name">Hutchinson, Joseph</span> and <span class="person_name">Hilder, Emily</span> and <span class="person_name">Jacobson, Glenn</span> (2008) <xhtml:em>A simple capillary electrophoresis method for the rapid separation and determination of intact low molecular weight and unfractionated heparins.</xhtml:em> Journal of Pharmaceutical and Biomedical Analysis, 46 (1). pp. 30-35. ISSN 0731-7085</p><p style="margin-bottom: 1em" class="not_ep_block"></p><table style="margin-bottom: 1em" class="not_ep_block"><tr><td valign="top" style="text-align:center"><a href="http://eprints.utas.edu.au/2706/1/0030_-_0035_Patel.pdf"><img alt="[img]" src="http://eprints.utas.edu.au/style/images/fileicons/application_pdf.png" class="ep_doc_icon" border="0" /></a></td><td valign="top"><a href="http://eprints.utas.edu.au/2706/1/0030_-_0035_Patel.pdf"><span class="ep_document_citation">PDF</span></a> - Full text restricted until January 2008 - Requires a PDF viewer<br />340Kb</td><td><form method="get" accept-charset="utf-8" action="http://eprints.utas.edu.au/cgi/request_doc"><input accept-charset="utf-8" value="3582" name="docid" type="hidden" /><div class=""><input value="Request a copy" name="_action_null" class="ep_form_action_button" onclick="return EPJS_button_pushed( '_action_null' )" type="submit" /> </div></form></td></tr></table><p style="margin-bottom: 1em" class="not_ep_block">Official URL: <a href="http://dx.doi.org/10.1016/j.jpba.2007.10.009">http://dx.doi.org/10.1016/j.jpba.2007.10.009</a></p><div class="not_ep_block"><h2>Abstract</h2><p style="padding-bottom: 16px; text-align: left; margin: 1em auto 0em auto">A simple, selective and accurate capillary electrophoresis (CE) method has been developed for the rapid separation and identification of various
low molecular weight heparins (LMWHs) and unfractionated heparin. Separation and operational parameters were investigated using dalteparin
sodium as the test LMWH. The developed method used a 70 cm fused silica capillary (50 m i.d.) with a detection window 8.5 cm from the
distal end. Phosphate electrolyte (pH 3.5; 50 mM), an applied voltage of −30 kV, UV detection at 230 nm and sample injection at 20 mbar for 5 s
were used. The method performance was assessed in terms of linearity, selectivity, intra- and inter-day precision and accuracy. The method was
successfully applied to the European Pharmacopeia LMWH standard, dalteparin sodium, enoxaparin sodium and heparin sodium with a significant
reduction in the run time and increased resolution compared with previously reported CE methods. Different CE separation profiles were obtained
for various LMWHs and unfractionated heparin showing significant structural diversity. The current methodology was sensitive enough to reveal
minor constituent differences between two different batches of enoxaparin sodium. This CE method also clearly showed chemical changes that
occurred to LMWHs under different stress conditions. The sensitivity, selectivity and simplicity of the developed method allow its application in
research or manufacturing for the identification, stability analysis, characterization and monitoring of batch-to-batch consistency of different low
molecular weight and unfractionated heparins.
© 2007 Elsevier B.V. All rights reserved.</p></div><table style="margin-bottom: 1em" cellpadding="3" class="not_ep_block" border="0"><tr><th valign="top" class="ep_row">Item Type:</th><td valign="top" class="ep_row">Article</td></tr><tr><th valign="top" class="ep_row">Additional Information:</th><td valign="top" class="ep_row">The definitive version is available at http://www.sciencedirect.com
</td></tr><tr><th valign="top" class="ep_row">Keywords:</th><td valign="top" class="ep_row">Low molecular weight heparin; Dalteparin; Heparin; Capillary electrophoresis; Batch-to-batch variations</td></tr><tr><th valign="top" class="ep_row">Subjects:</th><td valign="top" class="ep_row"><a href="http://eprints.utas.edu.au/view/subjects/320501.html">320000 Medical and Health Sciences > 320500 Pharmacology and Pharmaceutical Sciences > 320501 Pharmaceutical Sciences and Pharmacy</a></td></tr><tr><th valign="top" class="ep_row">ID Code:</th><td valign="top" class="ep_row">2706</td></tr><tr><th valign="top" class="ep_row">Deposited By:</th><td valign="top" class="ep_row"><span class="ep_name_citation"><span class="person_name">Mrs Gina Hadolt</span></span></td></tr><tr><th valign="top" class="ep_row">Deposited On:</th><td valign="top" class="ep_row">13 Dec 2007 15:50</td></tr><tr><th valign="top" class="ep_row">Last Modified:</th><td valign="top" class="ep_row">09 Jan 2008 02:30</td></tr><tr><th valign="top" class="ep_row">ePrint Statistics:</th><td valign="top" class="ep_row"><a target="ePrintStats" href="/es/index.php?action=show_detail_eprint;id=2706;">View statistics for this ePrint</a></td></tr></table><p align="right">Repository Staff Only: <a href="http://eprints.utas.edu.au/cgi/users/home?screen=EPrint::View&eprintid=2706">item control page</a></p>
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