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    <title>UTas ePrints - Bacterial decontamination of on-grown Artemia</title>
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<meta content="The bacterial load of on-grown Artemia was manipulated using a variety of commercially available enrichment DHA boosters, selected algal species (Skeletonema costatum; Nannochloropsis oculata; Tetraselmis suecica; Chaetoceros muelleri), and ozone to decontaminate enteric and external surfaces, respectively. Enrichment in C. muelleri over a 6-h period, with an additional algal exchange mid-enrichment, provided the most efficient method for enteric decontamination as measured by total viable counts. Direct exposure to ozone at 4 ppm for 5 min provided further bacterial reduction, resulting in a combined bacterial load reduction of 99.5% without compromising Artemia viability. The commercial enrichment A1 DHA Selco™, containing antibacterial compounds, provides an alternative to algal-based enrichments, however, its decontaminating properties were inferior. Fluorescence in situ hybridisation (FISH) was used on occasions to verify total bacterial abundance estimates obtained by standard plating procedures. In all except one case, Johnson's Marine Agar (JMA) provided results comparable to direct counts by FISH. This indicates that the bacterial community present in on-grown Artemia cultures is dominated by several fast-growing r-strategists amenable to culture on conventional plates. Underestimation of bacterial abundance using marine agar was therefore unlikely. " name="eprints.abstract" />
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<meta content="Amann, R.I., Ludwig, W. and Schleifer, K.H., 1995. Phylogenetic identification and in situ detection of individual microbial cells without cultivation. Microbiol. Rev. 59, pp. 143-169. View Record in Scopus | Cited By in Scopus (2777)

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De Wolf, T., Dehasque, M. and Coutteau, P., 1998. Intensive hygenic Artemia production. Bull.-Aquac. Assoc. Can. 4, pp. 25-26.

Dobbeleir, J., Adam, N., Bossuyt, E., Bruggeman, E. and Sorgeloos, P., 1980. New aspects of the use of inert diets for high density culturing of brine shrimp. In: Persoone, G., Sorgeloos, P., Roels, O. and Jaspers, E., Editors, 1980. The Brine Shrimp ArtemiaEcology, Culturing, Use in Aquaculture, Universa Press, Wetteren, Belgium, pp. 165-174.

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Glockner, F.O., Amann, R., Alfreider, A., Pernthaler, J., Psenner, R., Trebesius, K. and Schleifer, K.H., 1996. An in situ hybridization protocol for detection and identification of planktonic bacteria. Syst. Appl. Microbiol. 19, pp. 403-406. View Record in Scopus | Cited By in Scopus (148)

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Lopez-Torres, M.A. and Lizarraga-Partida, M.L., 2001. Bacteria isolated on TCBS media associated with hatched Artemia cysts of commercial brands. Aquaculture 194, pp. 11-20. SummaryPlus | Full Text + Links | PDF (74 K) | View Record in Scopus | Cited By in Scopus (7)

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Olsen, A.I., Olsen, Y., Attramadal, Y., Christie, K., Birkbeck, T.H., Skjermo, J. and Vadstein, O., 2000. Effects of short term feeding of microalgae on the bacterial flora associated with juvenile Artemia franciscana. Aquaculture 190, pp. 11-25. SummaryPlus | Full Text + Links | PDF (129 K) | View Record in Scopus | Cited By in Scopus (12)

Otta, S.K., Shubha, G., Joseph, B., Chakraborty, A., Karunasagar, I. and Karunasagar, I., 1999. Polymerase chain reaction (PCR) detection of white spot syndrome virus (WSSV) in cultured and wild crustaceans in India. Dis. Aquat. Org. 38, pp. 67-70. View Record in Scopus | Cited By in Scopus (32)

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Smith, G.G., Ritar, A.J., Phleger, C.F., Nelson, M.M., Mooney, B., Nichols, P.D. and Hart, P.R., 2002. Changes in gut content and composition of juvenile Artemia after oil enrichment and during starvation. Aquaculture 208, pp. 137-158. SummaryPlus | Full Text + Links | PDF (251 K) | View Record in Scopus | Cited By in Scopus (11)

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Verdonck, L., Swings, J., Kersters, K., Dehasque, M., Sorgeloos, P. and Leger, P., 1994. Variability of the microbial environment of rotifer Brachionus plicatilus and Artemia production systems. J. World Aquac. Soc. 25, pp. 55-59. Full Text via CrossRef 

Verschuere, L., Dhont, J., Sorgeloos, P. and Verstraete, W., 1997. Monitoring biolog patterns and r/K-strategists in the intensive culture of Artemia juveniles. J. Appl. Microbiol. 83, pp. 603-612. View Record in Scopus | Cited By in Scopus (21)

Verschuere, L., Rombaut, G., Sorgeloos, P. and Verstraete, W., 2000. Probiotic bacteria as biological control agents in aquaculture. Microbiol. Mol. Biol. Rev. 64, pp. 655-671. Full Text via CrossRef | View Record in Scopus | Cited By in Scopus (99)

Verschuere, L., Heang, H., Criel, G., Sorgeloos, P. and Verstraete, W., 2000. Selected bacterial strains protect Artemia spp. from the pathogenic effects of Vibrio proteolyticus CW8T2. Appl. Environ. Microbiol. 66, pp. 1139-1146. Full Text via CrossRef | View Record in Scopus | Cited By in Scopus (23) " name="eprints.referencetext" />
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    <h1 class="ep_tm_pagetitle">Bacterial decontamination of on-grown Artemia</h1>
    <p style="margin-bottom: 1em" class="not_ep_block"><span class="person_name">Tolomei, Anthony</span> and <span class="person_name">Burke, Chris</span> and <span class="person_name">Crear, Bradley J.</span> and <span class="person_name">Carson, Jeremy</span> (2004) <xhtml:em>Bacterial decontamination of on-grown Artemia.</xhtml:em> Aquaculture, 232 (1-4). pp. 357-371.</p><p style="margin-bottom: 1em" class="not_ep_block"></p><table style="margin-bottom: 1em" class="not_ep_block"><tr><td valign="top" style="text-align:center"><a href="http://eprints.utas.edu.au/1767/1/Tolomei_etal_04.pdf"><img alt="[img]" src="http://eprints.utas.edu.au/style/images/fileicons/application_pdf.png" class="ep_doc_icon" border="0" /></a></td><td valign="top"><a href="http://eprints.utas.edu.au/1767/1/Tolomei_etal_04.pdf"><span class="ep_document_citation">PDF</span></a> - Full text restricted - Requires a PDF viewer<br />388Kb</td></tr></table><p style="margin-bottom: 1em" class="not_ep_block">Official URL: <a href="http://dx.doi.org/10.1016/S0044-8486(03)00540-4">http://dx.doi.org/10.1016/S0044-8486(03)00540-4</a></p><div class="not_ep_block"><h2>Abstract</h2><p style="padding-bottom: 16px; text-align: left; margin: 1em auto 0em auto">The bacterial load of on-grown Artemia was manipulated using a variety of commercially available enrichment DHA boosters, selected algal species (Skeletonema costatum; Nannochloropsis oculata; Tetraselmis suecica; Chaetoceros muelleri), and ozone to decontaminate enteric and external surfaces, respectively. Enrichment in C. muelleri over a 6-h period, with an additional algal exchange mid-enrichment, provided the most efficient method for enteric decontamination as measured by total viable counts. Direct exposure to ozone at 4 ppm for 5 min provided further bacterial reduction, resulting in a combined bacterial load reduction of 99.5% without compromising Artemia viability. The commercial enrichment A1 DHA Selco™, containing antibacterial compounds, provides an alternative to algal-based enrichments, however, its decontaminating properties were inferior. Fluorescence in situ hybridisation (FISH) was used on occasions to verify total bacterial abundance estimates obtained by standard plating procedures. In all except one case, Johnson's Marine Agar (JMA) provided results comparable to direct counts by FISH. This indicates that the bacterial community present in on-grown Artemia cultures is dominated by several fast-growing r-strategists amenable to culture on conventional plates. Underestimation of bacterial abundance using marine agar was therefore unlikely. </p></div><table style="margin-bottom: 1em" cellpadding="3" class="not_ep_block" border="0"><tr><th valign="top" class="ep_row">Item Type:</th><td valign="top" class="ep_row">Article</td></tr><tr><th valign="top" class="ep_row">Additional Information:</th><td valign="top" class="ep_row">The definitive version is available at http://www.sciencedirect.com/</td></tr><tr><th valign="top" class="ep_row">Keywords:</th><td valign="top" class="ep_row">Larviculture; Disinfection; Vector; Vibrio; Probiotics </td></tr><tr><th valign="top" class="ep_row">Subjects:</th><td valign="top" class="ep_row"><a href="http://eprints.utas.edu.au/view/subjects/300507.html">300000 Agricultural, Veterinary and Environmental Sciences &gt; 300500 Veterinary Sciences &gt; 300507 Microbiology (excl. Virology)</a><br /><a href="http://eprints.utas.edu.au/view/subjects/300703.html">300000 Agricultural, Veterinary and Environmental Sciences &gt; 300700 Fisheries Sciences &gt; 300703 Aquaculture</a></td></tr><tr><th valign="top" class="ep_row">ID Code:</th><td valign="top" class="ep_row">1767</td></tr><tr><th valign="top" class="ep_row">Deposited By:</th><td valign="top" class="ep_row"><span class="ep_name_citation"><span class="person_name">Dr Chris Burke</span></span></td></tr><tr><th valign="top" class="ep_row">Deposited On:</th><td valign="top" class="ep_row">06 Sep 2007</td></tr><tr><th valign="top" class="ep_row">Last Modified:</th><td valign="top" class="ep_row">09 Jan 2008 02:30</td></tr><tr><th valign="top" class="ep_row">ePrint Statistics:</th><td valign="top" class="ep_row"><a target="ePrintStats" href="/es/index.php?action=show_detail_eprint;id=1767;">View statistics for this ePrint</a></td></tr></table><p align="right">Repository Staff Only: <a href="http://eprints.utas.edu.au/cgi/users/home?screen=EPrint::View&amp;eprintid=1767">item control page</a></p>
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