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  5.     <title>UTas ePrints - Genetic expression profile of olfactory ensheathing cells is distinct from that of Schwann cells and astrocytes</title>
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  13.     <meta content="Vincent, A.J." name="eprints.creators_name" />
  14. <meta content="Taylor, J.M." name="eprints.creators_name" />
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  27. <meta content="Genetic expression profile of olfactory ensheathing cells is distinct from that of Schwann cells and astrocytes" name="eprints.title" />
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  33. <meta content="Olfactory ensheathing cells (OECs) accompany the axons of
  34. olfactory receptor neurons, which regenerate throughout life,
  35. from the olfactory mucosa into the olfactory bulb. OECs have
  36. shown widely varying efficacy in repairing the injured nervous
  37. system. Analysis of the transcriptome of OECs will help in
  38. understanding their biology and will provide tools for investigating
  39. the mechanisms of their efficacy and interactions with
  40. host tissues in lesion models. In this study, we compared the
  41. transcriptional profile of cultured OECs with that of Schwann
  42. cells (SCs) and astrocytes (ACs), two glial cell types to which
  43. OECs have similarities. Two biological replicates of RNA from
  44. cultured OECs, SCs, and ACs were hybridized to long oligo rat
  45. 5K arrays against a common reference pool of RNA (50% cultured
  46. fibroblast RNA and 50% neonatal rat brain RNA). Transcriptional
  47. profiles were analyzed by hierarchical clustering,
  48. Principal Components Analysis, and the Venn diagram. The
  49. three glial cell types had similarly increased or decreased
  50. expression of numerous transcripts compared with the reference.
  51. However, OECs were distinguishable from both SCs and
  52. ACs by a modest number of transcripts, which were significantly
  53. enriched or depleted. Furthermore, OECs and SCs were
  54. more closely related to each other than to ACs. Expression of
  55. selected transcripts not previously characterized in OECs,
  56. such as Lyz, Timp2, Gro1 (Cxcl1), Ccl2 (MCP1), Ctgf, and
  57. Cebpb, was validated by real-time reverse transcription-polymerase
  58. chain reaction (RT-PCR); immunohistochemistry in
  59. cultured OECs, SCs, and ACs, and adult tissues was performed
  60. to demonstrate their expression at the protein level." name="eprints.abstract" />
  61. <meta content="2005" name="eprints.date" />
  62. <meta content="published" name="eprints.date_type" />
  63. <meta content="Glia" name="eprints.publication" />
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  65. <meta content="132-147" name="eprints.pagerange" />
  66. <meta content="10.1002/glia.20195" name="eprints.id_number" />
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  68. <meta content="0894-1491" name="eprints.issn" />
  69. <meta content="http://dx.doi.org/10.1002/glia.20195" name="eprints.official_url" />
  70. <meta content="Babcock AA, Kuziel WA, Rivest S, Owens T. 2003. Chemokine expression
  71. by glial cells directs leukocytes to sites of axonal injury in the
  72. CNS. J Neurosci 23:7922–7930.
  73. Barnett SC, Riddell JS. 2004. Olfactory ensheathing cells (OECs) and
  74. the treatment of CNS injury: advantages and possible caveats. J
  75. Anat 204:57–67.
  76. Barnett SC, Thompson RJ, Lakatos A, Pitts J. 2001. Gap junctional
  77. communication and connexin expression in cultured olfactory
  78. ensheathing cells. J Neurosci Res 65:520–528.
  79. Brockes JP, Fields KL, Raff MC. 1979. Studies on cultured rat Schwann
  80. cells. I. Establishment of purified populations from cultures of peripheral
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  82. Chuah MI, Teague R. 1999. Basic fibroblast growth factor in the primary
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  84. 88:1043–1050.
  85. Chuah MI, Cossins J, Woodhall E, Tennent R, Nash G, West AK. 2000.
  86. Glial growth factor 2 induces proliferation and structural changes in
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  88. Chuah MI, Choi-Lundberg D, Weston S, Vincent AJ, Chung RS, Vickers
  89. JC, West AK. 2004. Olfactory ensheathing cells promote collateral
  90. axonal branching in the injured adult rat spinal cord. Exp Neurol
  91. 185:15–25.
  92. Devon R, Doucette R. 1995. Olfactory ensheathing cells do not require
  93. L-ascorbic acid in vitro to assemble a basal lamina or to myelinate
  94. dorsal root ganglion neurites. Brain Res 688:223–229.
  95. Doucette R. 1990. Glial influences on axonal growth in the primary
  96. olfactory system. Glia 3:433–449.
  97. Franceschini IA, Barnett SC. 1996. Low-affinity NGF-receptor and EN-
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  99. cells that share a common lineage. Dev Biol 173:327–343.
  100. Franklin RJ. 2002. Remyelination of the demyelinated CNS: the case
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  104. cell-like myelination following transplantation of an olfactory bulbensheathing
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  108. Acute transplantation of olfactory ensheathing cells or Schwann cells
  109. promotes recovery after spinal cord injury in the rat. J Neurosci Res
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  120. Babbo A, Cohn SL. 2000. Schwann cell-conditioned medium inhibits
  121. angiogenesis. Cancer Res 60:5966–5971.
  122. Imaizumi T, Lankford KL, Waxman SG, Greer CA, Kocsis JD. 1998.
  123. Transplanted olfactory ensheathing cells remyelinate and enhance
  124. axonal conduction in the demyelinated dorsal columns of the rat spinal
  125. cord. J Neurosci 18:6176–6185.
  126. Imaizumi T, Lankford KL, Burton WV, Fodor WL, Kocsis JD. 2000.
  127. Xenotransplantation of transgenic pig olfactory ensheathing cells promotes
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  137. induce less host astrocyte response and chondroitin sulphate proteoglycan
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  139. adult CNS white matter. Exp Neurol 184:237–246.
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  141. by transplants of olfactory ensheathing cells. Science 277:2000–2002.
  142. Li Y, Carlstedt T, Berthold CH, Raisman G. 2004. Interaction of transplanted
  143. olfactory-ensheathing cells and host astrocytic processes provides
  144. a bridge for axons to regenerate across the dorsal root entry
  145. zone. Exp Neurol 188:300–308.
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  157. factor, interleukin-6, and their receptors are expressed transiently in
  158. the olfactory mucosa after target ablation. J Comp Neurol 435:60–77.
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  167. by transplantation of H-transferase transgenic adult pig olfactory
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  169. Rajeevan MS, Vernon SD, Taysavang N, Unger ER. 2001. Validation of
  170. array-based gene expression profiles by real-time (kinetic) RT-PCR. J
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  172. Ramer LM, Richter MW, Roskams AJ, Tetzlaff W, Ramer MS. 2004.
  173. Peripherally-derived olfactory ensheathing cells do not promote primary
  174. afferent regeneration following dorsal root injury. Glia 47:189–206.
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  176. cord of transected dorsal root axons is promoted by ensheathing glia
  177. transplants. Exp Neurol 127:232–244.
  178. Ramon-Cueto A, Plant GW, Avila J, Bunge MB. 1998. Long-distance
  179. axonal regeneration in the transected adult rat spinal cord is promoted
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  183. recovery of paraplegic rats and motor axon regeneration in
  184. their spinal cords by olfactory ensheathing glia. Neuron 25:425–435.
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  227. demyelinating multiple sclerosis lesions. Am J Pathol 154:45–51.
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  229. Briles DE. 2003. Pneumococcal carriage results in ganglioside-mediated
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  233. olfactory ensheathing cells is regulated by cAMP and endothelin-1.
  234. Glia 41:393–403.
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  239. cells express nerve growth factor, brain- derived neurotrophic factor,
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  242. Woodhall E, West AK, Vickers JC, Chuah MI. 2003. Olfactory ensheathing
  243. cell phenotype following implantation in the lesioned spinal cord.
  244. Cell Mol Life Sci 60:2241–2253." name="eprints.referencetext" />
  245. <meta content="Vincent, A.J. and Taylor, J.M. and Choi-Lundberg, D.L. and West, A.K. and Chuah, Meng Inn (2005) Genetic expression profile of olfactory ensheathing cells is distinct from that of Schwann cells and astrocytes. Glia, 51 . pp. 132-147. ISSN 0894-1491" name="eprints.citation" />
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  249. <meta content="Vincent, A.J." name="DC.creator" />
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  253. <meta content="Chuah, Meng Inn" name="DC.creator" />
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  255. <meta content="Olfactory ensheathing cells (OECs) accompany the axons of
  256. olfactory receptor neurons, which regenerate throughout life,
  257. from the olfactory mucosa into the olfactory bulb. OECs have
  258. shown widely varying efficacy in repairing the injured nervous
  259. system. Analysis of the transcriptome of OECs will help in
  260. understanding their biology and will provide tools for investigating
  261. the mechanisms of their efficacy and interactions with
  262. host tissues in lesion models. In this study, we compared the
  263. transcriptional profile of cultured OECs with that of Schwann
  264. cells (SCs) and astrocytes (ACs), two glial cell types to which
  265. OECs have similarities. Two biological replicates of RNA from
  266. cultured OECs, SCs, and ACs were hybridized to long oligo rat
  267. 5K arrays against a common reference pool of RNA (50% cultured
  268. fibroblast RNA and 50% neonatal rat brain RNA). Transcriptional
  269. profiles were analyzed by hierarchical clustering,
  270. Principal Components Analysis, and the Venn diagram. The
  271. three glial cell types had similarly increased or decreased
  272. expression of numerous transcripts compared with the reference.
  273. However, OECs were distinguishable from both SCs and
  274. ACs by a modest number of transcripts, which were significantly
  275. enriched or depleted. Furthermore, OECs and SCs were
  276. more closely related to each other than to ACs. Expression of
  277. selected transcripts not previously characterized in OECs,
  278. such as Lyz, Timp2, Gro1 (Cxcl1), Ccl2 (MCP1), Ctgf, and
  279. Cebpb, was validated by real-time reverse transcription-polymerase
  280. chain reaction (RT-PCR); immunohistochemistry in
  281. cultured OECs, SCs, and ACs, and adult tissues was performed
  282. to demonstrate their expression at the protein level." name="DC.description" />
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  396.     <h1 class="ep_tm_pagetitle">Genetic expression profile of olfactory ensheathing cells is distinct from that of Schwann cells and astrocytes</h1>
  397.     <p style="margin-bottom: 1em" class="not_ep_block"><span class="person_name">Vincent, A.J.</span> and <span class="person_name">Taylor, J.M.</span> and <span class="person_name">Choi-Lundberg, D.L.</span> and <span class="person_name">West, A.K.</span> and <span class="person_name">Chuah, Meng Inn</span> (2005) <xhtml:em>Genetic expression profile of olfactory ensheathing cells is distinct from that of Schwann cells and astrocytes.</xhtml:em> Glia, 51 . pp. 132-147. ISSN 0894-1491</p><p style="margin-bottom: 1em" class="not_ep_block"></p><table style="margin-bottom: 1em" class="not_ep_block"><tr><td valign="top" style="text-align:center"><a href="http://eprints.utas.edu.au/2231/1/Vincent_et_al_2005_Glia.pdf"><img alt="[img]" src="http://eprints.utas.edu.au/style/images/fileicons/application_pdf.png" class="ep_doc_icon" border="0" /></a></td><td valign="top"><a href="http://eprints.utas.edu.au/2231/1/Vincent_et_al_2005_Glia.pdf"><span class="ep_document_citation">PDF</span></a> - Full text restricted - Requires a PDF viewer<br />937Kb</td><td><form method="get" accept-charset="utf-8" action="http://eprints.utas.edu.au/cgi/request_doc"><input accept-charset="utf-8" value="2801" name="docid" type="hidden" /><div class=""><input value="Request a copy" name="_action_null" class="ep_form_action_button" onclick="return EPJS_button_pushed( '_action_null' )" type="submit" /> </div></form></td></tr></table><p style="margin-bottom: 1em" class="not_ep_block">Official URL: <a href="http://dx.doi.org/10.1002/glia.20195">http://dx.doi.org/10.1002/glia.20195</a></p><div class="not_ep_block"><h2>Abstract</h2><p style="padding-bottom: 16px; text-align: left; margin: 1em auto 0em auto">Olfactory ensheathing cells (OECs) accompany the axons of&#13;
  398. olfactory receptor neurons, which regenerate throughout life,&#13;
  399. from the olfactory mucosa into the olfactory bulb. OECs have&#13;
  400. shown widely varying efficacy in repairing the injured nervous&#13;
  401. system. Analysis of the transcriptome of OECs will help in&#13;
  402. understanding their biology and will provide tools for investigating&#13;
  403. the mechanisms of their efficacy and interactions with&#13;
  404. host tissues in lesion models. In this study, we compared the&#13;
  405. transcriptional profile of cultured OECs with that of Schwann&#13;
  406. cells (SCs) and astrocytes (ACs), two glial cell types to which&#13;
  407. OECs have similarities. Two biological replicates of RNA from&#13;
  408. cultured OECs, SCs, and ACs were hybridized to long oligo rat&#13;
  409. 5K arrays against a common reference pool of RNA (50% cultured&#13;
  410. fibroblast RNA and 50% neonatal rat brain RNA). Transcriptional&#13;
  411. profiles were analyzed by hierarchical clustering,&#13;
  412. Principal Components Analysis, and the Venn diagram. The&#13;
  413. three glial cell types had similarly increased or decreased&#13;
  414. expression of numerous transcripts compared with the reference.&#13;
  415. However, OECs were distinguishable from both SCs and&#13;
  416. ACs by a modest number of transcripts, which were significantly&#13;
  417. enriched or depleted. Furthermore, OECs and SCs were&#13;
  418. more closely related to each other than to ACs. Expression of&#13;
  419. selected transcripts not previously characterized in OECs,&#13;
  420. such as Lyz, Timp2, Gro1 (Cxcl1), Ccl2 (MCP1), Ctgf, and&#13;
  421. Cebpb, was validated by real-time reverse transcription-polymerase&#13;
  422. chain reaction (RT-PCR); immunohistochemistry in&#13;
  423. cultured OECs, SCs, and ACs, and adult tissues was performed&#13;
  424. to demonstrate their expression at the protein level.</p></div><table style="margin-bottom: 1em" cellpadding="3" class="not_ep_block" border="0"><tr><th valign="top" class="ep_row">Item Type:</th><td valign="top" class="ep_row">Article</td></tr><tr><th valign="top" class="ep_row">Additional Information:</th><td valign="top" class="ep_row">see individual journal copyright transfer agreements</td></tr><tr><th valign="top" class="ep_row">Keywords:</th><td valign="top" class="ep_row">microarray; real-time RT-PCR; cytokines; lysozyme; glia</td></tr><tr><th valign="top" class="ep_row">Subjects:</th><td valign="top" class="ep_row"><a href="http://eprints.utas.edu.au/view/subjects/270000.html">270000 Biological Sciences</a></td></tr><tr><th valign="top" class="ep_row">ID Code:</th><td valign="top" class="ep_row">2231</td></tr><tr><th valign="top" class="ep_row">Deposited By:</th><td valign="top" class="ep_row"><span class="ep_name_citation"><span class="person_name">A/Prof MI Chuah</span></span></td></tr><tr><th valign="top" class="ep_row">Deposited On:</th><td valign="top" class="ep_row">17 Oct 2007 14:01</td></tr><tr><th valign="top" class="ep_row">Last Modified:</th><td valign="top" class="ep_row">09 Jan 2008 02:30</td></tr><tr><th valign="top" class="ep_row">ePrint Statistics:</th><td valign="top" class="ep_row"><a target="ePrintStats" href="/es/index.php?action=show_detail_eprint;id=2231;">View statistics for this ePrint</a></td></tr></table><p align="right">Repository Staff Only: <a href="http://eprints.utas.edu.au/cgi/users/home?screen=EPrint::View&amp;eprintid=2231">item control page</a></p>
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