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<title>UTas ePrints - Genetic expression profile of olfactory ensheathing cells is distinct from that of Schwann cells and astrocytes</title>
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<meta content="Vincent, A.J." name="eprints.creators_name" />
<meta content="Taylor, J.M." name="eprints.creators_name" />
<meta content="Choi-Lundberg, D.L." name="eprints.creators_name" />
<meta content="West, A.K." name="eprints.creators_name" />
<meta content="Chuah, Meng Inn" name="eprints.creators_name" />
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<meta content="Inn.Chuah@utas.edu.au" name="eprints.creators_id" />
<meta content="article" name="eprints.type" />
<meta content="2007-10-17 03:01:41" name="eprints.datestamp" />
<meta content="2008-01-08 15:30:00" name="eprints.lastmod" />
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<meta content="Genetic expression profile of olfactory ensheathing cells is distinct from that of Schwann cells and astrocytes" name="eprints.title" />
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<meta content="270000" name="eprints.subjects" />
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<meta content="microarray; real-time RT-PCR; cytokines; lysozyme; glia" name="eprints.keywords" />
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<meta content="Olfactory ensheathing cells (OECs) accompany the axons of
olfactory receptor neurons, which regenerate throughout life,
from the olfactory mucosa into the olfactory bulb. OECs have
shown widely varying efficacy in repairing the injured nervous
system. Analysis of the transcriptome of OECs will help in
understanding their biology and will provide tools for investigating
the mechanisms of their efficacy and interactions with
host tissues in lesion models. In this study, we compared the
transcriptional profile of cultured OECs with that of Schwann
cells (SCs) and astrocytes (ACs), two glial cell types to which
OECs have similarities. Two biological replicates of RNA from
cultured OECs, SCs, and ACs were hybridized to long oligo rat
5K arrays against a common reference pool of RNA (50% cultured
fibroblast RNA and 50% neonatal rat brain RNA). Transcriptional
profiles were analyzed by hierarchical clustering,
Principal Components Analysis, and the Venn diagram. The
three glial cell types had similarly increased or decreased
expression of numerous transcripts compared with the reference.
However, OECs were distinguishable from both SCs and
ACs by a modest number of transcripts, which were significantly
enriched or depleted. Furthermore, OECs and SCs were
more closely related to each other than to ACs. Expression of
selected transcripts not previously characterized in OECs,
such as Lyz, Timp2, Gro1 (Cxcl1), Ccl2 (MCP1), Ctgf, and
Cebpb, was validated by real-time reverse transcription-polymerase
chain reaction (RT-PCR); immunohistochemistry in
cultured OECs, SCs, and ACs, and adult tissues was performed
to demonstrate their expression at the protein level." name="eprints.abstract" />
<meta content="2005" name="eprints.date" />
<meta content="published" name="eprints.date_type" />
<meta content="Glia" name="eprints.publication" />
<meta content="51" name="eprints.volume" />
<meta content="132-147" name="eprints.pagerange" />
<meta content="10.1002/glia.20195" name="eprints.id_number" />
<meta content="TRUE" name="eprints.refereed" />
<meta content="0894-1491" name="eprints.issn" />
<meta content="http://dx.doi.org/10.1002/glia.20195" name="eprints.official_url" />
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Cell Mol Life Sci 60:2241–2253." name="eprints.referencetext" />
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<meta content="Olfactory ensheathing cells (OECs) accompany the axons of
olfactory receptor neurons, which regenerate throughout life,
from the olfactory mucosa into the olfactory bulb. OECs have
shown widely varying efficacy in repairing the injured nervous
system. Analysis of the transcriptome of OECs will help in
understanding their biology and will provide tools for investigating
the mechanisms of their efficacy and interactions with
host tissues in lesion models. In this study, we compared the
transcriptional profile of cultured OECs with that of Schwann
cells (SCs) and astrocytes (ACs), two glial cell types to which
OECs have similarities. Two biological replicates of RNA from
cultured OECs, SCs, and ACs were hybridized to long oligo rat
5K arrays against a common reference pool of RNA (50% cultured
fibroblast RNA and 50% neonatal rat brain RNA). Transcriptional
profiles were analyzed by hierarchical clustering,
Principal Components Analysis, and the Venn diagram. The
three glial cell types had similarly increased or decreased
expression of numerous transcripts compared with the reference.
However, OECs were distinguishable from both SCs and
ACs by a modest number of transcripts, which were significantly
enriched or depleted. Furthermore, OECs and SCs were
more closely related to each other than to ACs. Expression of
selected transcripts not previously characterized in OECs,
such as Lyz, Timp2, Gro1 (Cxcl1), Ccl2 (MCP1), Ctgf, and
Cebpb, was validated by real-time reverse transcription-polymerase
chain reaction (RT-PCR); immunohistochemistry in
cultured OECs, SCs, and ACs, and adult tissues was performed
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<h1 class="ep_tm_pagetitle">Genetic expression profile of olfactory ensheathing cells is distinct from that of Schwann cells and astrocytes</h1>
<p style="margin-bottom: 1em" class="not_ep_block"><span class="person_name">Vincent, A.J.</span> and <span class="person_name">Taylor, J.M.</span> and <span class="person_name">Choi-Lundberg, D.L.</span> and <span class="person_name">West, A.K.</span> and <span class="person_name">Chuah, Meng Inn</span> (2005) <xhtml:em>Genetic expression profile of olfactory ensheathing cells is distinct from that of Schwann cells and astrocytes.</xhtml:em> Glia, 51 . pp. 132-147. ISSN 0894-1491</p><p style="margin-bottom: 1em" class="not_ep_block"></p><table style="margin-bottom: 1em" class="not_ep_block"><tr><td valign="top" style="text-align:center"><a href="http://eprints.utas.edu.au/2231/1/Vincent_et_al_2005_Glia.pdf"><img alt="[img]" src="http://eprints.utas.edu.au/style/images/fileicons/application_pdf.png" class="ep_doc_icon" border="0" /></a></td><td valign="top"><a href="http://eprints.utas.edu.au/2231/1/Vincent_et_al_2005_Glia.pdf"><span class="ep_document_citation">PDF</span></a> - Full text restricted - Requires a PDF viewer<br />937Kb</td><td><form method="get" accept-charset="utf-8" action="http://eprints.utas.edu.au/cgi/request_doc"><input accept-charset="utf-8" value="2801" name="docid" type="hidden" /><div class=""><input value="Request a copy" name="_action_null" class="ep_form_action_button" onclick="return EPJS_button_pushed( '_action_null' )" type="submit" /> </div></form></td></tr></table><p style="margin-bottom: 1em" class="not_ep_block">Official URL: <a href="http://dx.doi.org/10.1002/glia.20195">http://dx.doi.org/10.1002/glia.20195</a></p><div class="not_ep_block"><h2>Abstract</h2><p style="padding-bottom: 16px; text-align: left; margin: 1em auto 0em auto">Olfactory ensheathing cells (OECs) accompany the axons of
olfactory receptor neurons, which regenerate throughout life,
from the olfactory mucosa into the olfactory bulb. OECs have
shown widely varying efficacy in repairing the injured nervous
system. Analysis of the transcriptome of OECs will help in
understanding their biology and will provide tools for investigating
the mechanisms of their efficacy and interactions with
host tissues in lesion models. In this study, we compared the
transcriptional profile of cultured OECs with that of Schwann
cells (SCs) and astrocytes (ACs), two glial cell types to which
OECs have similarities. Two biological replicates of RNA from
cultured OECs, SCs, and ACs were hybridized to long oligo rat
5K arrays against a common reference pool of RNA (50% cultured
fibroblast RNA and 50% neonatal rat brain RNA). Transcriptional
profiles were analyzed by hierarchical clustering,
Principal Components Analysis, and the Venn diagram. The
three glial cell types had similarly increased or decreased
expression of numerous transcripts compared with the reference.
However, OECs were distinguishable from both SCs and
ACs by a modest number of transcripts, which were significantly
enriched or depleted. Furthermore, OECs and SCs were
more closely related to each other than to ACs. Expression of
selected transcripts not previously characterized in OECs,
such as Lyz, Timp2, Gro1 (Cxcl1), Ccl2 (MCP1), Ctgf, and
Cebpb, was validated by real-time reverse transcription-polymerase
chain reaction (RT-PCR); immunohistochemistry in
cultured OECs, SCs, and ACs, and adult tissues was performed
to demonstrate their expression at the protein level.</p></div><table style="margin-bottom: 1em" cellpadding="3" class="not_ep_block" border="0"><tr><th valign="top" class="ep_row">Item Type:</th><td valign="top" class="ep_row">Article</td></tr><tr><th valign="top" class="ep_row">Additional Information:</th><td valign="top" class="ep_row">see individual journal copyright transfer agreements</td></tr><tr><th valign="top" class="ep_row">Keywords:</th><td valign="top" class="ep_row">microarray; real-time RT-PCR; cytokines; lysozyme; glia</td></tr><tr><th valign="top" class="ep_row">Subjects:</th><td valign="top" class="ep_row"><a href="http://eprints.utas.edu.au/view/subjects/270000.html">270000 Biological Sciences</a></td></tr><tr><th valign="top" class="ep_row">ID Code:</th><td valign="top" class="ep_row">2231</td></tr><tr><th valign="top" class="ep_row">Deposited By:</th><td valign="top" class="ep_row"><span class="ep_name_citation"><span class="person_name">A/Prof MI Chuah</span></span></td></tr><tr><th valign="top" class="ep_row">Deposited On:</th><td valign="top" class="ep_row">17 Oct 2007 14:01</td></tr><tr><th valign="top" class="ep_row">Last Modified:</th><td valign="top" class="ep_row">09 Jan 2008 02:30</td></tr><tr><th valign="top" class="ep_row">ePrint Statistics:</th><td valign="top" class="ep_row"><a target="ePrintStats" href="/es/index.php?action=show_detail_eprint;id=2231;">View statistics for this ePrint</a></td></tr></table><p align="right">Repository Staff Only: <a href="http://eprints.utas.edu.au/cgi/users/home?screen=EPrint::View&eprintid=2231">item control page</a></p>
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