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  5.     <title>UTas ePrints - Respiratory actions of vanilloid receptor agonists in the nucleus of the solitary tract: comparison of resiniferatoxin with non-pungent agents and anandamide</title>
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  13.     <meta content="Geraghty, D.P." name="eprints.creators_name" />
  14. <meta content="Mazzone, S.B." name="eprints.creators_name" />
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  21. <meta content="Respiratory actions of vanilloid receptor agonists in the nucleus of the solitary tract: comparison of resiniferatoxin with non-pungent agents and anandamide" name="eprints.title" />
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  25. <meta content="Nucleus of the solitary tract; tachykinins; vanilloid; desensitization; C-®bre" name="eprints.keywords" />
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  28. <meta content="1 Activation of vanilloid receptors on sensory nerve terminals in the commissural nucleus of the
  29. solitary tract (cNTS) of rats with capsaicin, produces respiratory slowing. In this study, we used
  30. microinjection techniques employing pungent and non-pungent vanilloids to further characterize
  31. vanilloid receptors in the cNTS.
  32. 2 Microinjection of the pungent vanilloid, resiniferatoxin (RTX), into the cNTS of urethane-
  33. anaesthetized rats, dose-dependently reduced respiratory rate without a€ecting tidal volume. RTX
  34. was 20 fold more potent at slowing respiration (*ED50, 100 pmol) than capsaicin (*ED50, 2 nmol).
  35. Doses of RTX greater than 100 pmol caused either irregular (dyspnoeic) breathing or terminal
  36. apnoea (4250 pmol). The respiratory slowing response to RTX (75 pmol), was dose-dependently
  37. attenuated by injecting RTX (but not vehicle) into the same site 60 min earlier.
  38. 3 The non-pungent phorbol derivative of RTX, phorbol 12-phenylacetete 13-acetate 20-
  39. homovanillate (PPAHV, 0.1 - 1 nmol), also slowed respiration (ED50, *1 nmol) and almost
  40. abolished response to RTX (75 pmol) injected into the same site 60 min later.
  41. 4 In contrast to RTX, PPAHV and capsaicin, the putative endogenous vanilloid receptor agonist,
  42. arachidonyl ethanolamide (AEA), and non-pungent capsaicin derivative, olvanil, had no direct e€ect
  43. on respiration. However, both AEA and olvanil dose-dependently reduced the respiratory response
  44. to injection of RTX (75 pmol) 60 min later into the same site (EC50s, for AEA and olvanil, *2 and
  45. 0.2 nmol, respectively).
  46. 5 These studies suggest that both pungent and non-pungent vanilloids interact with vanilloid
  47. receptors in the cNTS. However, whereas RTX and PPAHV activate and subsequently desensitize
  48. vanilloid receptors on sensory nerve terminals in the cNTS, olvanil and AEA fail to activate despite
  49. readily desensitizing responses to RTX in this region" name="eprints.abstract" />
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  55. <meta content="919-927" name="eprints.pagerange" />
  56. <meta content="10.1038/sj.bjp.0704931" name="eprints.id_number" />
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  215. " name="eprints.referencetext" />
  216. <meta content="Geraghty, D.P. and Mazzone, S.B. (2002) Respiratory actions of vanilloid receptor agonists in the nucleus of the solitary tract: comparison of resiniferatoxin with non-pungent agents and anandamide. British Journal of Pharmacology, 137 (6). pp. 919-927. ISSN 0007-1188" name="eprints.citation" />
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  219. <meta content="Respiratory actions of vanilloid receptor agonists in the nucleus of the solitary tract: comparison of resiniferatoxin with non-pungent agents and anandamide" name="DC.title" />
  220. <meta content="Geraghty, D.P." name="DC.creator" />
  221. <meta content="Mazzone, S.B." name="DC.creator" />
  222. <meta content="320502 Basic Pharmacology" name="DC.subject" />
  223. <meta content="1 Activation of vanilloid receptors on sensory nerve terminals in the commissural nucleus of the
  224. solitary tract (cNTS) of rats with capsaicin, produces respiratory slowing. In this study, we used
  225. microinjection techniques employing pungent and non-pungent vanilloids to further characterize
  226. vanilloid receptors in the cNTS.
  227. 2 Microinjection of the pungent vanilloid, resiniferatoxin (RTX), into the cNTS of urethane-
  228. anaesthetized rats, dose-dependently reduced respiratory rate without a€ecting tidal volume. RTX
  229. was 20 fold more potent at slowing respiration (*ED50, 100 pmol) than capsaicin (*ED50, 2 nmol).
  230. Doses of RTX greater than 100 pmol caused either irregular (dyspnoeic) breathing or terminal
  231. apnoea (4250 pmol). The respiratory slowing response to RTX (75 pmol), was dose-dependently
  232. attenuated by injecting RTX (but not vehicle) into the same site 60 min earlier.
  233. 3 The non-pungent phorbol derivative of RTX, phorbol 12-phenylacetete 13-acetate 20-
  234. homovanillate (PPAHV, 0.1 - 1 nmol), also slowed respiration (ED50, *1 nmol) and almost
  235. abolished response to RTX (75 pmol) injected into the same site 60 min later.
  236. 4 In contrast to RTX, PPAHV and capsaicin, the putative endogenous vanilloid receptor agonist,
  237. arachidonyl ethanolamide (AEA), and non-pungent capsaicin derivative, olvanil, had no direct e€ect
  238. on respiration. However, both AEA and olvanil dose-dependently reduced the respiratory response
  239. to injection of RTX (75 pmol) 60 min later into the same site (EC50s, for AEA and olvanil, *2 and
  240. 0.2 nmol, respectively).
  241. 5 These studies suggest that both pungent and non-pungent vanilloids interact with vanilloid
  242. receptors in the cNTS. However, whereas RTX and PPAHV activate and subsequently desensitize
  243. vanilloid receptors on sensory nerve terminals in the cNTS, olvanil and AEA fail to activate despite
  244. readily desensitizing responses to RTX in this region" name="DC.description" />
  245. <meta content="2002-11" name="DC.date" />
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  358.     <h1 class="ep_tm_pagetitle">Respiratory actions of vanilloid receptor agonists in the nucleus of the solitary tract: comparison of resiniferatoxin with non-pungent agents and anandamide</h1>
  359.     <p style="margin-bottom: 1em" class="not_ep_block"><span class="person_name">Geraghty, D.P.</span> and <span class="person_name">Mazzone, S.B.</span> (2002) <xhtml:em>Respiratory actions of vanilloid receptor agonists in the nucleus of the solitary tract: comparison of resiniferatoxin with non-pungent agents and anandamide.</xhtml:em> British Journal of Pharmacology, 137 (6). pp. 919-927. ISSN 0007-1188</p><p style="margin-bottom: 1em" class="not_ep_block"></p><table style="margin-bottom: 1em" class="not_ep_block"><tr><td valign="top" style="text-align:center"><a href="http://eprints.utas.edu.au/2254/1/15._BJP_2002.pdf"><img alt="[img]" src="http://eprints.utas.edu.au/style/images/fileicons/application_pdf.png" class="ep_doc_icon" border="0" /></a></td><td valign="top"><a href="http://eprints.utas.edu.au/2254/1/15._BJP_2002.pdf"><span class="ep_document_citation">PDF</span></a> - Full text restricted - Requires a PDF viewer<br />235Kb</td><td><form method="get" accept-charset="utf-8" action="http://eprints.utas.edu.au/cgi/request_doc"><input accept-charset="utf-8" value="2836" name="docid" type="hidden" /><div class=""><input value="Request a copy" name="_action_null" class="ep_form_action_button" onclick="return EPJS_button_pushed( '_action_null' )" type="submit" /> </div></form></td></tr></table><p style="margin-bottom: 1em" class="not_ep_block">Official URL: <a href="http://dx.doi.org/10.1038/sj.bjp.0704931">http://dx.doi.org/10.1038/sj.bjp.0704931</a></p><div class="not_ep_block"><h2>Abstract</h2><p style="padding-bottom: 16px; text-align: left; margin: 1em auto 0em auto">1 Activation of vanilloid receptors on sensory nerve terminals in the commissural nucleus of the&#13;
  360. solitary tract (cNTS) of rats with capsaicin, produces respiratory slowing. In this study, we used&#13;
  361. microinjection techniques employing pungent and non-pungent vanilloids to further characterize&#13;
  362. vanilloid receptors in the cNTS.&#13;
  363. 2 Microinjection of the pungent vanilloid, resiniferatoxin (RTX), into the cNTS of urethane-&#13;
  364. anaesthetized rats, dose-dependently reduced respiratory rate without a€ecting tidal volume. RTX&#13;
  365. was 20 fold more potent at slowing respiration (*ED50, 100 pmol) than capsaicin (*ED50, 2 nmol).&#13;
  366. Doses of RTX greater than 100 pmol caused either irregular (dyspnoeic) breathing or terminal&#13;
  367. apnoea (4250 pmol). The respiratory slowing response to RTX (75 pmol), was dose-dependently&#13;
  368. attenuated by injecting RTX (but not vehicle) into the same site 60 min earlier.&#13;
  369. 3 The non-pungent phorbol derivative of RTX, phorbol 12-phenylacetete 13-acetate 20-&#13;
  370. homovanillate (PPAHV, 0.1 - 1 nmol), also slowed respiration (ED50, *1 nmol) and almost&#13;
  371. abolished response to RTX (75 pmol) injected into the same site 60 min later.&#13;
  372. 4 In contrast to RTX, PPAHV and capsaicin, the putative endogenous vanilloid receptor agonist,&#13;
  373. arachidonyl ethanolamide (AEA), and non-pungent capsaicin derivative, olvanil, had no direct e€ect&#13;
  374. on respiration. However, both AEA and olvanil dose-dependently reduced the respiratory response&#13;
  375. to injection of RTX (75 pmol) 60 min later into the same site (EC50s, for AEA and olvanil, *2 and&#13;
  376. 0.2 nmol, respectively).&#13;
  377. 5 These studies suggest that both pungent and non-pungent vanilloids interact with vanilloid&#13;
  378. receptors in the cNTS. However, whereas RTX and PPAHV activate and subsequently desensitize&#13;
  379. vanilloid receptors on sensory nerve terminals in the cNTS, olvanil and AEA fail to activate despite&#13;
  380. readily desensitizing responses to RTX in this region</p></div><table style="margin-bottom: 1em" cellpadding="3" class="not_ep_block" border="0"><tr><th valign="top" class="ep_row">Item Type:</th><td valign="top" class="ep_row">Article</td></tr><tr><th valign="top" class="ep_row">Additional Information:</th><td valign="top" class="ep_row">The definitive version is available at www.blackwell-synergy.com&#13;
  381. </td></tr><tr><th valign="top" class="ep_row">Keywords:</th><td valign="top" class="ep_row">Nucleus of the solitary tract; tachykinins; vanilloid; desensitization; C-®bre</td></tr><tr><th valign="top" class="ep_row">Subjects:</th><td valign="top" class="ep_row"><a href="http://eprints.utas.edu.au/view/subjects/320502.html">320000 Medical and Health Sciences &gt; 320500 Pharmacology and Pharmaceutical Sciences &gt; 320502 Basic Pharmacology</a></td></tr><tr><th valign="top" class="ep_row">ID Code:</th><td valign="top" class="ep_row">2254</td></tr><tr><th valign="top" class="ep_row">Deposited By:</th><td valign="top" class="ep_row"><span class="ep_name_citation"><span class="person_name">Assoc Prof Dominic P Geraghty</span></span></td></tr><tr><th valign="top" class="ep_row">Deposited On:</th><td valign="top" class="ep_row">19 Oct 2007 14:43</td></tr><tr><th valign="top" class="ep_row">Last Modified:</th><td valign="top" class="ep_row">09 Jan 2008 02:30</td></tr><tr><th valign="top" class="ep_row">ePrint Statistics:</th><td valign="top" class="ep_row"><a target="ePrintStats" href="/es/index.php?action=show_detail_eprint;id=2254;">View statistics for this ePrint</a></td></tr></table><p align="right">Repository Staff Only: <a href="http://eprints.utas.edu.au/cgi/users/home?screen=EPrint::View&amp;eprintid=2254">item control page</a></p>
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