Newer
Older
Digital_Repository / Misc / Mass downloads / UTas / 2432.html
  1. <!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN"
  2. "http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd">
  3. <html>
  4. <head>
  5. <title>UTas ePrints - Combined effects of feeding enriched rotifers and antibiotic addition on performance of striped trumpeter (Latris lineata) larvae</title>
  6. <script type="text/javascript" src="http://eprints.utas.edu.au/javascript/auto.js"><!-- padder --></script>
  7. <style type="text/css" media="screen">@import url(http://eprints.utas.edu.au/style/auto.css);</style>
  8. <style type="text/css" media="print">@import url(http://eprints.utas.edu.au/style/print.css);</style>
  9. <link rel="icon" href="/images/eprints/favicon.ico" type="image/x-icon" />
  10. <link rel="shortcut icon" href="/images/eprints/favicon.ico" type="image/x-icon" />
  11. <link rel="Top" href="http://eprints.utas.edu.au/" />
  12. <link rel="Search" href="http://eprints.utas.edu.au/cgi/search" />
  13. <meta content="Battaglene, Stephen C." name="eprints.creators_name" />
  14. <meta content="Morehead, David T." name="eprints.creators_name" />
  15. <meta content="Cobcroft, J.M." name="eprints.creators_name" />
  16. <meta content="Nichols, Peter D." name="eprints.creators_name" />
  17. <meta content="Brown, M.R." name="eprints.creators_name" />
  18. <meta content="Carson, Jeremy" name="eprints.creators_name" />
  19. <meta content="Stephen.Battaglene@utas.edu" name="eprints.creators_id" />
  20. <meta content="" name="eprints.creators_id" />
  21. <meta content="" name="eprints.creators_id" />
  22. <meta content="" name="eprints.creators_id" />
  23. <meta content="" name="eprints.creators_id" />
  24. <meta content="" name="eprints.creators_id" />
  25. <meta content="article" name="eprints.type" />
  26. <meta content="2007-11-12 00:55:03" name="eprints.datestamp" />
  27. <meta content="2008-01-08 15:30:00" name="eprints.lastmod" />
  28. <meta content="show" name="eprints.metadata_visibility" />
  29. <meta content="Combined effects of feeding enriched rotifers and antibiotic
  30. addition on performance of striped trumpeter (Latris lineata) larvae" name="eprints.title" />
  31. <meta content="pub" name="eprints.ispublished" />
  32. <meta content="restricted" name="eprints.full_text_status" />
  33. <meta content="Latris lineata; Microheterotrophs; DHA; EPA; Antibiotic; Lipid; Marine fish larvae; Bacteria; Fatty acid" name="eprints.keywords" />
  34. <meta content="The definitive version is available at http://www.sciencedirect.com
  35. " name="eprints.note" />
  36. <meta content="Striped trumpeter have a complex and extended larval phase and are difficult to culture. Two experiments were
  37. conducted in replicated, 300-l hemispherical tanks to determine if larval survival, growth, bacterial or fatty acid profile
  38. were improved by feeding non-enriched rotifers or rotifers enriched with algae or commercially available products, as well
  39. as the effect of an antibiotic, oxytetracycline (OTC). Larvae were stocked at 25 l1 and 15 l1 and reared until Day 16
  40. and Day 19 in Experiments 1 and 2, respectively. In Experiment 1, the feeding treatments were non-enriched rotifers, or
  41. rotifers enriched on algae, DHA Selco (a fish oil based emulsion) or RotiMac (dried Schizochytrium). There were no
  42. significant differences in mean survival (FSD) across treatments, which were generally low at 14.6F5.2%. Larvae reared
  43. on rotifers fed DHA Selco and RotiMac, had significantly higher proportions of incorporated DHA but no significant
  44. increase in growth. In Experiment 2, larvae were fed rotifers enriched on DHA Selco or AlgaMac 2000 and reared with or
  45. without the daily addition of 25 mg l1 OTC. At Day 19, there was significantly higher survival for larvae reared on
  46. AlgaMac 2000 and OTC, (37.4F5.6%), than DHA Selco and OTC, (16.0F7.4%), AlgaMac 2000 without OTC
  47. (7.0F8.0) and DHA Selco without OTC (3.3F1.2). Larvae reared with OTC were larger (279F58 Ag and 7.4F0.2
  48. mm) than without OTC (177F40 Ag and 6.3F0.2 mm). The addition of antibiotics did not significantly influence fatty
  49. acid profiles of larvae. There were no significant differences in the percentage of DHA, 27.6F2.8%, EPA 4.6F1.0% or
  50. ARA 4.9F0.4%. Larvae reared with OTC had significantly less dgrey gutT (a measure of intestinal dysfunction). The
  51. results indicated that bacterial infection was a major source of mortality in striped trumpeter larvae and compromised larval
  52. growth. Assessment of the bacterial flora indicated that antibiotic use reduced the bacterial load, but did not eliminate" name="eprints.abstract" />
  53. <meta content="2006" name="eprints.date" />
  54. <meta content="published" name="eprints.date_type" />
  55. <meta content="Aquaculture" name="eprints.publication" />
  56. <meta content="251" name="eprints.volume" />
  57. <meta content="2 - 4" name="eprints.number" />
  58. <meta content="456-471" name="eprints.pagerange" />
  59. <meta content="10.1016/j.aquaculture.2005.06.021" name="eprints.id_number" />
  60. <meta content="TRUE" name="eprints.refereed" />
  61. <meta content="0044-8486" name="eprints.issn" />
  62. <meta content="http://dx.doi.org/10.1016/j.aquaculture.2005.06.021" name="eprints.official_url" />
  63. <meta content="Angulo, L., Lopez, J.E., Vicente, J.A., Saborido, A.M., 1994.
  64. Haemorrhagic areas in the mouth of farmed turbot, Scophthalmus
  65. maximus (L.). J. Fish Dis. 17, 163– 169.
  66. Battaglene, S.C., Morehead, D.M., Nichols, P.D., Brown, M.,
  67. Carson, J., 2002. Why are striped trumpeter (Latris lineata)
  68. larvae so difficult to culture—is it lipid nutrition? 26th Annual
  69. Larval Fish Conference. American Fisheries Society, Bergen,
  70. Norway.
  71. Battaglene, S., Ritar, R., Purser, J., Bolch, C., 2004. Priorities for
  72. live feed production and research in Tasmania. In: Kolkovski,
  73. S., Heine, J., Clarke, S. (Eds.), The Second Hatchery Feeds and
  74. Technology Workshop. Department Fisheries, Western Australia,
  75. pp. 104– 112.
  76. Bligh, E.G., Dyer, W.G., 1959. A rapid method of total lipid
  77. extraction and purification. Can. J. Biochem. Physiol. 37,
  78. 911– 917.
  79. Bransden, M.P., Dunstan, G.A., Battaglene, S.C., Cobcroft, J.M.,
  80. Morehead, D.T., Kolkovski, S., Nichols, P.D., 2004. Influences
  81. of dietary n3 long chain PUFA on body concentrations of
  82. 20:5 n3, 22:5 n3 and 22:6 n3 in larvae of a marine
  83. teleost fish from Australian waters, the striped trumpeter (Latris
  84. lineata). Lipids 39, 215– 222.
  85. Bransden, M.P., Cobcroft, J.M., Battaglene, S.C., Dunstan, G.A.,
  86. Nichols, P.D., Bell, J.G., in press a. Dietary arachidonic acid
  87. alters tissue fatty acid profile, whole body eicosanoid production
  88. and stress resistance in larvae of the temperate
  89. marine fish, striped trumpeter (Latris lineata). Fish Physiol.
  90. Biochem.
  91. Bransden, M.P., Cobcroft, J.M., Battaglene, S.C., Morehead, D.T.,
  92. Dunstan, G.A., Nichols, P.D., Kolkovski, S., in press b. Dietary
  93. 22:6 n3 alters gut and liver structure and behaviour in larval
  94. striped trumpeter (Latris lineata). Aquaculture.
  95. Brown, M.R., Lavens, P., 2001. Critical review of the concentration,
  96. interactions with other nutrients, and transfer of ascorbic acid in
  97. algae, crustaceans and fish. In: Dabrowski, K. (Ed.), Ascorbic
  98. Acid in Aquatic Organisms: Status and Perspectives. CRC
  99. Press, Boca Raton FL USA, pp. 167– 189.
  100. Brown, M.R., Skabo, S., Wilkinson, B., 1998. The enrichment and
  101. retention of ascorbic acid in rotifers fed microalgal diets. Aquac.
  102. Nutr. 4, 151– 156.
  103. Brown, M.R., Dunstan, G.A., Nichols, P.D., Battaglene, S.C., Morehead,
  104. D.T., Overweter, A.O., 2005. Effects of a-tocopherol
  105. supplementation of rotifers on the growth of striped trumpeter
  106. Latris lineata larvae. Aquaculture 246, 367–378.
  107. Brown, M.R., Battaglene, S.C., Morehead, D.T., Brock, M., in
  108. press. Ontogenetic changes in amino acid and vitamins during
  109. early larval stages of striped trumpeter (Latris lineata).
  110. Aquaculture.
  111. Bryant, T., 2004. PIBWin-Software for probabilistic identification.
  112. J. Appl. Microbiol. 97, 1326– 1327.
  113. Cobcroft, J.M., 2002. Aspects of the morphological development
  114. and feeding performance of larval striped trumpeter (Latris
  115. lineata) in culture. PhD thesis, School of Aquaculture, University
  116. of Tasmania, Launceston, p. 194.
  117. Cobcroft, J.M., Pankhurst, P.M., Sadler, J., Hart, P.R., 2001. Jaw
  118. development and malformation in cultured striped trumpeter
  119. Latris lineata. Aquaculture 199, 267– 282.
  120. Farto, R., Montes, M., Perez, M.J., Nieto, T.P., Larsen, J.L., Pedersen,
  121. K., 1999. Characterization by numerical taxonomy and
  122. ribotyping of Vibrio splendidus biovar I and Vibrio scophthalmi
  123. strains associated with turbot cultures. J. Appl. Microbiol. 86,
  124. 796– 804.
  125. Furlani, D.M., Ruwald, F.P., 1999. Egg and larval development of
  126. laboratory-reared striped trumpeter Latris lineata (Forster in
  127. Bloch and Schneider 1801) (Percoidei: Latridiidae) from Tasmanian
  128. waters. N.Z. J. Mar. Freshw. Res. 33, 153– 162.
  129. Furuita, H., Takeuchi, T., Watanabe, T., Fujimoto, H., Sekiya, S.,
  130. Imaizumi, K., 1996. Requirements of larval yellowtail for eicosapentaenoic
  131. acid, docosahexaenoic acid, and n3 highly unsaturated
  132. fatty acid. Fish. Sci. 62, 372–379.
  133. Garrity, G.M., Bell, J.A., Lilburn, T.G., 2004. Taxonomic outline of
  134. the prokaryotes, Bergey’s Manual of Systematic Bacteriology,
  135. Second edition Springer-Verlag, New York, doi: 10.1007/bergeysoutline200405
  136. release 5.0.
  137. Gatesoupe, F., Lambert, C., Nicolas, J., 1999. Pathogenicity of
  138. Vibrio splendidus strains associated with turbot larvae
  139. Scophthalmus maximus. J. Appl. Microbiol. 87, 757– 763.
  140. Gay, M., Berthe, F.C.J., Le Roux, F., 2004. Screening of Vibrio
  141. isolates to develop an experimental infection model in the
  142. Pacific oyster, Crassostrea gigas. Dis. Aquat. Org. 59, 49– 56.
  143. Hansen, G.H., Bergh, Ø., Michaelsen, J., Knappskog, D., 1992.
  144. Flexibacter ovolyticus sp. nov., a pathogen of eggs and larvae of
  145. Atlantic halibut, Hippoglossus hippoglossus L.. Int. J. Syst.
  146. Bacteriol. 42, 451– 458.
  147. Harel, M., Koven, W., Lein, I., Bar, Y., Behrens, P., Stubblefield, J.,
  148. Zohar, Y., Place, A.R., 2002. Advanced DHA, EPA and ArA
  149. enrichment materials for marine aquaculture using single cell
  150. heterotrophs. Aquaculture 213, 347– 362.
  151. Herna´ndez-Cruz, C.M., Salhi, M., Ferna´ndez-Palacios, H.,
  152. Izquierdo, M.S., 1994. Improvements in the culture of Sparus
  153. aurata L. larvae in relation to the use of antibiotics, phytoplankton
  154. and rearing system. Aquaculture 124, 269–274.
  155. Howell, B.R., Day, O.J., Ellis, T., Baynes, S.M., 1998. Early life
  156. stages of farmed fish. In: Black, K.D., Pickering, A.D. (Eds.),
  157. Biology of Farmed Fish. Sheffield Academic Press, Sheffield,
  158. pp. 27– 66.
  159. Izquierdo, M.S., 1996. Essential fatty acid requirements of cultured
  160. marine fish larvae. Aquac. Nutr. 2, 183–191.
  161. Izquierdo, M.S., Socorro, J., Arantzamendi, L., Herna´ndez-Cruz,
  162. C.M., 2000. Recent advances in lipid nutrition in fish larvae.
  163. Fish Physiol. Biochem. 22, 97–107.1996.
  164.  
  165. Johnson, P.T., 1968. A new medium for maintenance of marine
  166. bacteria. J. Invertebr. Pathol. 11, 144.
  167. Kjørsvik, E., Pittman, K., Pavlov, D., 2004. From fertilisation to the
  168. end of metamorphosis—functional development. In: Moksness,
  169. E., Kjorsvik, E., Olsen, Y. (Eds.), Culture of Cold-Water Marine
  170. Fish. Blackwell Publishing, Oxford, pp. 204– 278.
  171. Knuckey, R., Cox, E., 2004. Queensland department of Primary
  172. Industries and Fisheries. In: Kolkovski, S., Heine, J., Clarke, S.
  173. (Eds.), The Second Hatchery Feeds and Technology Workshop.
  174. WA Department of Fisheries, Sydney, pp. 64–68.
  175. Koven, W., Barr, Y., Lutzky, S., Ben-Atia, I., Weiss, R., Harel, M.,
  176. Behrens, P., Tandler, A., 2001. The effect of dietary arachidonic
  177. acid (20 :4 n6) on growth, survival and resistance to handling
  178. stress in gilthead seabream (Sparus aurata) larvae. Aquaculture
  179. 193, 107–122.
  180. Lein, I., Holmefjord, I., Rye, M., 1997. Effects of temperature on
  181. yolk sac larvae of Atlantic halibut (Hippoglossus hippoglossus
  182. L.). Aquaculture 157, 121–133.
  183. Lewis, T., Nichols, P.D., McMeekin, T.A., 2000. Evaluation
  184. of extraction methods for recovery of fatty acids from
  185. lipid-producing microheterotrophs. J. Microbiol. Methods 43,
  186. 107– 116.
  187. Le Roux, F., Gay, M., Lambert, C., Nicolas, J.L., Gouy, M., Berthe,
  188. F., 2004. Phylogenetic study and identification of Vibrio splendidus-
  189. related strains based on gyrB gene sequences. Dis. Aquat.
  190. Org. 58, 143– 150.
  191. Miles, A.A., Misra, S.S., 1938. The estimation of the bactericidal
  192. power of the blood. J. Hygiene, vol. 38. Cambridge,
  193. pp. 732–749.
  194. Moksness, E., Kjørsvik, E., Olsen, Y., 2004. Culture of Cold-Water
  195. Marine Fish. Blackwell Publishing, Oxford. 528 pp.
  196. Morehead, D.T., Hart, P.R., Dunstan, G.A., Brown, M., Pankhurst,
  197. N.W., 2001. Differences in egg quality between wild striped
  198. trumpeter (Latris lineata) and captive striped trumpeter that
  199. were fed different diets. Aquaculture 192, 39– 53.
  200. Moriarty, D.J.W., 1998. Control of luminous Vibrio species in
  201. penaeid aquaculture ponds. Aquaculture 164, 351– 358.
  202. Mourente, G., Rodriguez, A., Tocher, D.R., Sargent, J.R., 1993.
  203. Effects of dietary docosahexaenoic acid (DHA; 22:6 n3) on
  204. lipid and fatty acid compositions and growth in gilthead sea
  205. bream (Sparus aurata L.) larvae during first feeding. Aquaculture
  206. 112, 79–98.
  207. Munro, P.D., Birkbeck, T.H., Barbour, A., 1993. Influence of rate of
  208. bacterial colonisation of the gut of turbot larvae on larval
  209. survival. In: Reinersten, H., Dahle, L.A., Jørgensen, L., Tvinnereim,
  210. K. (Eds.), Fish Farming Technology. A A. Balkema,
  211. Rotterdam, pp. 85– 92.
  212. Munro, P., Barbour, A., Birkbeck, T., 1994. Comparison of the gut
  213. bacterial flora of start-feeding larval turbot reared under different
  214. conditions. J. Appl. Bacteriol. 77, 560–566.
  215. Munro, P.D., Barbour, A., Birkbeck, T.H., 1995. Comparison of
  216. the growth and survival of larval turbot in the absence of
  217. culturable bacteria with those in the presence of Vibrio alginolyticus
  218. or a marine Aeromonas sp. Appl. Environ. Microbiol.
  219. 61, 4425–4428.
  220. Nichols, D.S., Williams, R., Dunstan, G.A., Nichols, P.D., Volkman,
  221. J.K., 1994. Fatty acid composition of Antarctic and temperate
  222. fish of commercial interest. Comp. Biochem. Physiol. 107B,
  223. 357– 363.
  224. Nichols, P.D., Brock, M., Dunstan, G.A., Bransden, M., Goldsmid,
  225. R., Battaglene, S., 2005. A potential new Australian farmed
  226. seafood source of omega-3 oils: the oil and fatty acid composition
  227. of the finfish striped trumpeter (Latris lineata). Food
  228. Aust. 57, 295–301.
  229. Ottesen, O.H., Bolla, S., 1998. Combined effects of temperature and
  230. salinity on development and survival of Atlantic halibut larvae.
  231. Aquac. Int. 6, 103–120.
  232. Pankhurst, P.M., Hilder, P.E., 1998. Effect of light intensity on
  233. feeding of striped trumpeter Latris lineata larvae. Mar. Freshw.
  234. Res. 49, 363–368.
  235. Ringø, E., Birkbeck, T.H., 1999. Intestinal microflora of fish larvae
  236. and fry. Aquac. Res. 30, 73– 93.
  237. Rodriguez, C., Perez, J.A., Izquierdo, M.S., Mora, J., Lorenzo, A.,
  238. Fernandez-Palacios, H., 1994. Essential fatty acid requirements
  239. of larval gilthead sea bream Sparus aurata (L.). Aquac. Fish.
  240. Manage. 25, 295– 304.
  241. Ruwald, F.P., 1991. Larval feeding trials with striped trumpeter,
  242. Latris lineata. In: Hancock, D.A. (Ed.), Larval Biology Workshop.
  243. Australian Society for Fish Biology, Bureau of Rural
  244. Resources Proceedings No.15: 88.
  245. Salhi, M., Hernandez-Cruz, C.M., Bessonart, M., Izquierdo,
  246. M.S., Fernandez-Palacios, H., 1999. Effect of different dietary
  247. polar lipid levels and different n3 HUFA content in
  248. polar lipids on gut and liver histological structure of gilthead
  249. seabream (Sparus aurata) larvae. Aquaculture 179,
  250. 253– 263.
  251. Sargent, J.R., McEvoy, L.A., Estevez, A., Bell, J.G., Bell, M.V.,
  252. Henderson, R.J., Tocher, D.R., 1999. Lipid nutrition of marine
  253. fish during early development: current status and future directions.
  254. Aquaculture 179, 217– 229.
  255. Sargent, J.R., Tocher, D.R., Bell, J.G., 2002. The lipids. In: Halver,
  256. J.E., Hardy, R.W. (Eds.), Fish Nutrition. Academic Press, San
  257. Diego, USA, pp. 181– 257.
  258. Sedano, J., Zorrilla, I., Morinigo, M.A., Balebona, M.C., Vidaurreta,
  259. A., Bordas, M.A., Borrego, J.J., 1996. Microbial origin of
  260. the abdominal swelling affecting farmed larvae of gilt-head
  261. seabream Sparus aurata L.. Aquac. Res. 27, 323–333.
  262. Shelbourne, J.E., 1963. A marine fish-rearing experiment using
  263. antibiotics. Nature 198, 74– 75.
  264. Skjermo, J., Vadstein, O., 1999. Techniques for microbial control
  265. in the intensive rearing of marine larvae. Aquaculture 177,
  266. 333– 343.
  267. Thompson, F.L., Thompson, C.C., Li, Y., Gomez-Gil, B., Vandenberghe,
  268. J., Hoste, B., Swings, J., 2003. Vibrio kanaloae sp. nov.,
  269. Vibrio pomeroyi, sp. nov. and Vibrio chagasii sp. nov., from sea
  270. water and marine animals. Int. J. Syst. Evol. Microbiol. 53,
  271. 753– 759.
  272. Toranzo, A.E., Novoa, B., Romalde, J.L., Nu´n˜ez, S., Devesa, S.,
  273. Marin˜o, E., Silva, R., Martı´nez, E., Figueras, A., Barja, J.L.,
  274. 1993. Microflora associated with healthy and diseased turbot
  275. (Scophthalmus maximus) from three farms in northwest Spain.
  276. Aquaculture 114, 189– 202.
  277. Tracey, S.R., Lyle, J.M., 2005. Age validation, growth modeling
  278. and mortality estimates for striped trumpeter (Latris lineata)
  279.  
  280. from southeastern Australia: making the most of patchy data.
  281. Fish. Bull. 103, 169–182.
  282. Trotter, A.J., 2003. Swim bladder development and buoyancy control
  283. in cultured striped trumpeter (Latris lineata) larvae. PhD
  284. thesis, School of Aquaculture, University of Tasmania, Launceston,
  285. p. 155.
  286. Trotter, A.J., Pankhurst, P.M., Hart, P.R., 2001. Swim bladder
  287. malformation in hatchery-reared striped trumpeter Latris lineata
  288. (Latridae). Aquaculture 198, 41– 54.
  289. Trotter, A.J., Battaglene, S.C., Pankhurst, P.M., 2003a. Effects of
  290. photoperiod and light intensity on initial swim bladder inflation,
  291. growth and post-inflation viability in cultured striped trumpeter
  292. (Latris lineata) larvae. Aquaculture 224, 141– 158.
  293. Trotter, A.J., Pankhurst, P.M., Morehead, D.T., Battaglene, S.C.,
  294. 2003b. Effects of temperature on initial swim bladder inflation
  295. and related development in cultured striped trumpeter (Latris
  296. lineata) larvae. Aquaculture 221, 141–156.
  297. Vadstein, O., Mo, T.A., Bergh, Ø., 2004. Microbial interactions,
  298. prophylaxis and diseases. In: Moksness, E., Kjørsvik, E., Olsen,
  299. Y. (Eds.), Culture of Cold-Water Marine Fish. Blackwell Publishing,
  300. Oxford, pp. 28– 72.
  301. Verner-Jeffreys, D.W., Shields, R.J., Bricknell, I.R., Birkbeck, T.H.,
  302. 2004. Effects of different water treatment methods and antibiotic
  303. addition on larval survival and gut microflora development in
  304. Atlantic halibut (Hippoglossus hippoglossus L.) yolk-sac larvae.
  305. Aquaculture 232, 129–143.
  306. Volkman, J.K., Nichols, P.D., 1991. Application of thin layer chromatography-
  307. flame ionisation detection to the analysis of lipids
  308. and pollutants in marine and environmental samples. J. Planar
  309. Chromatogr.-Mod. TLC 4, 19–26.
  310. Watanabe, T., 1993. Importance of docosahexaenoic acid in marine
  311. larval fish. J. World Aquac. Soc. 24, 152– 161.
  312. Weston, D.P., 1996. Environmental considerations in the use of
  313. antibacterial drugs in aquaculture. In: Baird, D., Beveridge,
  314. M.V.M., Kelly, L.A., Muir, J.F. (Eds.), Aquaculture and Water
  315. Resource Management. Blackwell, Oxford, pp. 140– 165.
  316. Willcox, W., Lapage, S., Holmes, B., 1980. A review of numerical
  317. methods in bacterial identification. Antonie van Leeuwenhoek
  318. 46, 233– 299." name="eprints.referencetext" />
  319. <meta content="Battaglene, Stephen C. and Morehead, David T. and Cobcroft, J.M. and Nichols, Peter D. and Brown, M.R. and Carson, Jeremy (2006) Combined effects of feeding enriched rotifers and antibiotic addition on performance of striped trumpeter (Latris lineata) larvae. Aquaculture, 251 (2 - 4). pp. 456-471. ISSN 0044-8486" name="eprints.citation" />
  320. <meta content="http://eprints.utas.edu.au/2432/1/Battaglene_etal_antibiotiics.pdf" name="eprints.document_url" />
  321. <link rel="schema.DC" href="http://purl.org/DC/elements/1.0/" />
  322. <meta content="Combined effects of feeding enriched rotifers and antibiotic
  323. addition on performance of striped trumpeter (Latris lineata) larvae" name="DC.title" />
  324. <meta content="Battaglene, Stephen C." name="DC.creator" />
  325. <meta content="Morehead, David T." name="DC.creator" />
  326. <meta content="Cobcroft, J.M." name="DC.creator" />
  327. <meta content="Nichols, Peter D." name="DC.creator" />
  328. <meta content="Brown, M.R." name="DC.creator" />
  329. <meta content="Carson, Jeremy" name="DC.creator" />
  330. <meta content="Striped trumpeter have a complex and extended larval phase and are difficult to culture. Two experiments were
  331. conducted in replicated, 300-l hemispherical tanks to determine if larval survival, growth, bacterial or fatty acid profile
  332. were improved by feeding non-enriched rotifers or rotifers enriched with algae or commercially available products, as well
  333. as the effect of an antibiotic, oxytetracycline (OTC). Larvae were stocked at 25 l1 and 15 l1 and reared until Day 16
  334. and Day 19 in Experiments 1 and 2, respectively. In Experiment 1, the feeding treatments were non-enriched rotifers, or
  335. rotifers enriched on algae, DHA Selco (a fish oil based emulsion) or RotiMac (dried Schizochytrium). There were no
  336. significant differences in mean survival (FSD) across treatments, which were generally low at 14.6F5.2%. Larvae reared
  337. on rotifers fed DHA Selco and RotiMac, had significantly higher proportions of incorporated DHA but no significant
  338. increase in growth. In Experiment 2, larvae were fed rotifers enriched on DHA Selco or AlgaMac 2000 and reared with or
  339. without the daily addition of 25 mg l1 OTC. At Day 19, there was significantly higher survival for larvae reared on
  340. AlgaMac 2000 and OTC, (37.4F5.6%), than DHA Selco and OTC, (16.0F7.4%), AlgaMac 2000 without OTC
  341. (7.0F8.0) and DHA Selco without OTC (3.3F1.2). Larvae reared with OTC were larger (279F58 Ag and 7.4F0.2
  342. mm) than without OTC (177F40 Ag and 6.3F0.2 mm). The addition of antibiotics did not significantly influence fatty
  343. acid profiles of larvae. There were no significant differences in the percentage of DHA, 27.6F2.8%, EPA 4.6F1.0% or
  344. ARA 4.9F0.4%. Larvae reared with OTC had significantly less dgrey gutT (a measure of intestinal dysfunction). The
  345. results indicated that bacterial infection was a major source of mortality in striped trumpeter larvae and compromised larval
  346. growth. Assessment of the bacterial flora indicated that antibiotic use reduced the bacterial load, but did not eliminate" name="DC.description" />
  347. <meta content="2006" name="DC.date" />
  348. <meta content="Article" name="DC.type" />
  349. <meta content="PeerReviewed" name="DC.type" />
  350. <meta content="application/pdf" name="DC.format" />
  351. <meta content="http://eprints.utas.edu.au/2432/1/Battaglene_etal_antibiotiics.pdf" name="DC.identifier" />
  352. <meta content="http://dx.doi.org/10.1016/j.aquaculture.2005.06.021" name="DC.relation" />
  353. <meta content="Battaglene, Stephen C. and Morehead, David T. and Cobcroft, J.M. and Nichols, Peter D. and Brown, M.R. and Carson, Jeremy (2006) Combined effects of feeding enriched rotifers and antibiotic addition on performance of striped trumpeter (Latris lineata) larvae. Aquaculture, 251 (2 - 4). pp. 456-471. ISSN 0044-8486" name="DC.identifier" />
  354. <meta content="http://eprints.utas.edu.au/2432/" name="DC.relation" />
  355. <link rel="alternate" href="http://eprints.utas.edu.au/cgi/export/2432/BibTeX/epprod-eprint-2432.bib" title="BibTeX" type="text/plain" />
  356. <link rel="alternate" href="http://eprints.utas.edu.au/cgi/export/2432/ContextObject/epprod-eprint-2432.xml" title="OpenURL ContextObject" type="text/xml" />
  357. <link rel="alternate" href="http://eprints.utas.edu.au/cgi/export/2432/ContextObject::Dissertation/epprod-eprint-2432.xml" title="OpenURL Dissertation" type="text/xml" />
  358. <link rel="alternate" href="http://eprints.utas.edu.au/cgi/export/2432/ContextObject::Journal/epprod-eprint-2432.xml" title="OpenURL Journal" type="text/xml" />
  359. <link rel="alternate" href="http://eprints.utas.edu.au/cgi/export/2432/DC/epprod-eprint-2432.txt" title="Dublin Core" type="text/plain" />
  360. <link rel="alternate" href="http://eprints.utas.edu.au/cgi/export/2432/DIDL/epprod-eprint-2432.xml" title="DIDL" type="text/xml" />
  361. <link rel="alternate" href="http://eprints.utas.edu.au/cgi/export/2432/EndNote/epprod-eprint-2432.enw" title="EndNote" type="text/plain" />
  362. <link rel="alternate" href="http://eprints.utas.edu.au/cgi/export/2432/HTML/epprod-eprint-2432.html" title="HTML Citation" type="text/html; charset=utf-8" />
  363. <link rel="alternate" href="http://eprints.utas.edu.au/cgi/export/2432/METS/epprod-eprint-2432.xml" title="METS" type="text/xml" />
  364. <link rel="alternate" href="http://eprints.utas.edu.au/cgi/export/2432/MODS/epprod-eprint-2432.xml" title="MODS" type="text/xml" />
  365. <link rel="alternate" href="http://eprints.utas.edu.au/cgi/export/2432/RIS/epprod-eprint-2432.ris" title="Reference Manager" type="text/plain" />
  366. <link rel="alternate" href="http://eprints.utas.edu.au/cgi/export/2432/Refer/epprod-eprint-2432.refer" title="Refer" type="text/plain" />
  367. <link rel="alternate" href="http://eprints.utas.edu.au/cgi/export/2432/Simple/epprod-eprint-2432text" title="Simple Metadata" type="text/plain" />
  368. <link rel="alternate" href="http://eprints.utas.edu.au/cgi/export/2432/Text/epprod-eprint-2432.txt" title="ASCII Citation" type="text/plain; charset=utf-8" />
  369. <link rel="alternate" href="http://eprints.utas.edu.au/cgi/export/2432/XML/epprod-eprint-2432.xml" title="EP3 XML" type="text/xml" />
  370.  
  371. </head>
  372. <body bgcolor="#ffffff" text="#000000" onLoad="loadRoutine(); MM_preloadImages('images/eprints/ePrints_banner_r5_c5_f2.gif','images/eprints/ePrints_banner_r5_c7_f2.gif','images/eprints/ePrints_banner_r5_c8_f2.gif','images/eprints/ePrints_banner_r5_c9_f2.gif','images/eprints/ePrints_banner_r5_c10_f2.gif','images/eprints/ePrints_banner_r5_c11_f2.gif','images/eprints/ePrints_banner_r6_c4_f2.gif')">
  373. <div class="ep_noprint"><noscript><style type="text/css">@import url(http://eprints.utas.edu.au/style/nojs.css);</style></noscript></div>
  374.  
  375.  
  376.  
  377.  
  378. <table width="795" border="0" cellspacing="0" cellpadding="0">
  379. <tr>
  380. <td><script language="JavaScript1.2">mmLoadMenus();</script>
  381. <table border="0" cellpadding="0" cellspacing="0" width="795">
  382. <!-- fwtable fwsrc="eprints_banner_final2.png" fwbase="ePrints_banner.gif" fwstyle="Dreamweaver" fwdocid = "1249563342" fwnested="0" -->
  383. <tr>
  384. <td><img src="/images/eprints/spacer.gif" width="32" height="1" border="0" alt="" /></td>
  385. <td><img src="/images/eprints/spacer.gif" width="104" height="1" border="0" alt="" /></td>
  386. <td><img src="/images/eprints/spacer.gif" width="44" height="1" border="0" alt="" /></td>
  387. <td><img src="/images/eprints/spacer.gif" width="105" height="1" border="0" alt="" /></td>
  388. <td><img src="/images/eprints/spacer.gif" width="41" height="1" border="0" alt="" /></td>
  389. <td><img src="/images/eprints/spacer.gif" width="16" height="1" border="0" alt="" /></td>
  390. <td><img src="/images/eprints/spacer.gif" width="68" height="1" border="0" alt="" /></td>
  391. <td><img src="/images/eprints/spacer.gif" width="68" height="1" border="0" alt="" /></td>
  392. <td><img src="/images/eprints/spacer.gif" width="68" height="1" border="0" alt="" /></td>
  393. <td><img src="/images/eprints/spacer.gif" width="82" height="1" border="0" alt="" /></td>
  394. <td><img src="/images/eprints/spacer.gif" width="69" height="1" border="0" alt="" /></td>
  395. <td><img src="/images/eprints/spacer.gif" width="98" height="1" border="0" alt="" /></td>
  396. <td><img src="/images/eprints/spacer.gif" width="1" height="1" border="0" alt="" /></td>
  397. </tr>
  398. <tr>
  399. <td colspan="12"><img name="ePrints_banner_r1_c1" src="/images/eprints/ePrints_banner_r1_c1.gif" width="795" height="10" border="0" alt="" /></td>
  400. <td><img src="/images/eprints/spacer.gif" width="1" height="10" border="0" alt="" /></td>
  401. </tr>
  402. <tr>
  403. <td rowspan="6"><img name="ePrints_banner_r2_c1" src="/images/eprints/ePrints_banner_r2_c1.gif" width="32" height="118" border="0" alt="" /></td>
  404. <td rowspan="5"><a href="http://www.utas.edu.au/"><img name="ePrints_banner_r2_c2" src="/images/eprints/ePrints_banner_r2_c2.gif" width="104" height="103" border="0" alt="" /></a></td>
  405. <td colspan="10"><img name="ePrints_banner_r2_c3" src="/images/eprints/ePrints_banner_r2_c3.gif" width="659" height="41" border="0" alt="" /></td>
  406. <td><img src="/images/eprints/spacer.gif" width="1" height="41" border="0" alt="" /></td>
  407. </tr>
  408. <tr>
  409. <td colspan="3"><a href="http://eprints.utas.edu.au/"><img name="ePrints_banner_r3_c3" src="/images/eprints/ePrints_banner_r3_c3.gif" width="190" height="31" border="0" alt="" /></a></td>
  410. <td rowspan="2" colspan="7"><img name="ePrints_banner_r3_c6" src="/images/eprints/ePrints_banner_r3_c6.gif" width="469" height="37" border="0" alt="" /></td>
  411. <td><img src="/images/eprints/spacer.gif" width="1" height="31" border="0" alt="" /></td>
  412. </tr>
  413. <tr>
  414. <td colspan="3"><img name="ePrints_banner_r4_c3" src="/images/eprints/ePrints_banner_r4_c3.gif" width="190" height="6" border="0" alt="" /></td>
  415. <td><img src="/images/eprints/spacer.gif" width="1" height="6" border="0" alt="" /></td>
  416. </tr>
  417. <tr>
  418. <td colspan="2"><img name="ePrints_banner_r5_c3" src="/images/eprints/ePrints_banner_r5_c3.gif" width="149" height="1" border="0" alt="" /></td>
  419. <td rowspan="2" colspan="2"><a href="/information.html" onMouseOut="MM_swapImgRestore();MM_startTimeout()" onMouseOver="MM_showMenu(window.mm_menu_0821132634_0,0,25,null,'ePrints_banner_r5_c5');MM_swapImage('ePrints_banner_r5_c5','','/images/eprints/ePrints_banner_r5_c5_f2.gif',1);"><img name="ePrints_banner_r5_c5" src="/images/eprints/ePrints_banner_r5_c5.gif" width="57" height="25" border="0" alt="About" /></a></td>
  420. <td rowspan="2"><a href="/view/" onMouseOut="MM_swapImgRestore();MM_startTimeout()" onMouseOver="MM_showMenu(window.mm_menu_0821133021_1,0,25,null,'ePrints_banner_r5_c7');MM_swapImage('ePrints_banner_r5_c7','','/images/eprints/ePrints_banner_r5_c7_f2.gif',1);"><img name="ePrints_banner_r5_c7" src="/images/eprints/ePrints_banner_r5_c7.gif" width="68" height="25" border="0" alt="Browse" /></a></td>
  421. <td rowspan="2"><a href="/perl/search/simple" onMouseOut="MM_swapImgRestore();MM_startTimeout()" onMouseOver="MM_showMenu(window.mm_menu_0821133201_2,0,25,null,'ePrints_banner_r5_c8');MM_swapImage('ePrints_banner_r5_c8','','/images/eprints/ePrints_banner_r5_c8_f2.gif',1);"><img name="ePrints_banner_r5_c8" src="/images/eprints/ePrints_banner_r5_c8.gif" width="68" height="25" border="0" alt="Search" /></a></td>
  422. <td rowspan="2"><a href="/perl/register" onMouseOut="MM_swapImgRestore();MM_startTimeout();" onMouseOver="MM_showMenu(window.mm_menu_1018171924_3,0,25,null,'ePrints_banner_r5_c9');MM_swapImage('ePrints_banner_r5_c9','','/images/eprints/ePrints_banner_r5_c9_f2.gif',1);"><img name="ePrints_banner_r5_c9" src="/images/eprints/ePrints_banner_r5_c9.gif" width="68" height="25" border="0" alt="register" /></a></td>
  423. <td rowspan="2"><a href="/perl/users/home" onMouseOut="MM_swapImgRestore();MM_startTimeout()" onMouseOver="MM_showMenu(window.mm_menu_0821133422_4,0,25,null,'ePrints_banner_r5_c10');MM_swapImage('ePrints_banner_r5_c10','','/images/eprints/ePrints_banner_r5_c10_f2.gif',1);"><img name="ePrints_banner_r5_c10" src="/images/eprints/ePrints_banner_r5_c10.gif" width="82" height="25" border="0" alt="user area" /></a></td>
  424. <td rowspan="2"><a href="/help/" onMouseOut="MM_swapImgRestore();MM_startTimeout()" onMouseOver="MM_showMenu(window.mm_menu_0821133514_5,0,25,null,'ePrints_banner_r5_c11');MM_swapImage('ePrints_banner_r5_c11','','/images/eprints/ePrints_banner_r5_c11_f2.gif',1);"><img name="ePrints_banner_r5_c11" src="/images/eprints/ePrints_banner_r5_c11.gif" width="69" height="25" border="0" alt="Help" /></a></td>
  425. <td rowspan="3" colspan="4"><img name="ePrints_banner_r5_c12" src="/images/eprints/ePrints_banner_r5_c12.gif" width="98" height="40" border="0" alt="" /></td>
  426. <td><img src="/images/eprints/spacer.gif" width="1" height="1" border="0" alt="" /></td>
  427. </tr>
  428. <tr>
  429. <td rowspan="2"><img name="ePrints_banner_r6_c3" src="/images/eprints/ePrints_banner_r6_c3.gif" width="44" height="39" border="0" alt="ePrints home" /></td>
  430. <td><a href="/" onMouseOut="MM_swapImgRestore()" onMouseOver="MM_swapImage('ePrints_banner_r6_c4','','/images/eprints/ePrints_banner_r6_c4_f2.gif',1);"><img name="ePrints_banner_r6_c4" src="/images/eprints/ePrints_banner_r6_c4.gif" width="105" height="24" border="0" alt="ePrints home" /></a></td>
  431. <td><img src="/images/eprints/spacer.gif" width="1" height="24" border="0" alt="" /></td>
  432. </tr>
  433. <tr>
  434. <td><img name="ePrints_banner_r7_c2" src="/images/eprints/ePrints_banner_r7_c2.gif" width="104" height="15" border="0" alt="" /></td>
  435. <td colspan="8"><img name="ePrints_banner_r7_c4" src="/images/eprints/ePrints_banner_r7_c4.gif" width="517" height="15" border="0" alt="" /></td>
  436. <td><img src="/images/eprints/spacer.gif" width="1" height="15" border="0" alt="" /></td>
  437. </tr>
  438. </table></td>
  439. </tr>
  440. <tr><td><table width="100%" style="font-size: 90%; border: solid 1px #ccc; padding: 3px"><tr>
  441. <td align="left"><a href="http://eprints.utas.edu.au/cgi/users/home">Login</a> | <a href="http://eprints.utas.edu.au/cgi/register">Create Account</a></td>
  442. <td align="right" style="white-space: nowrap">
  443. <form method="get" accept-charset="utf-8" action="http://eprints.utas.edu.au/cgi/search" style="display:inline">
  444. <input class="ep_tm_searchbarbox" size="20" type="text" name="q" />
  445. <input class="ep_tm_searchbarbutton" value="Search" type="submit" name="_action_search" />
  446. <input type="hidden" name="_order" value="bytitle" />
  447. <input type="hidden" name="basic_srchtype" value="ALL" />
  448. <input type="hidden" name="_satisfyall" value="ALL" />
  449. </form>
  450. </td>
  451. </tr></table></td></tr>
  452. <tr>
  453. <td class="toplinks"><!-- InstanceBeginEditable name="content" -->
  454.  
  455.  
  456. <div align="center">
  457. <table width="720" class="ep_tm_main"><tr><td align="left">
  458. <h1 class="ep_tm_pagetitle">Combined effects of feeding enriched rotifers and antibiotic addition on performance of striped trumpeter (Latris lineata) larvae</h1>
  459. <p style="margin-bottom: 1em" class="not_ep_block"><span class="person_name">Battaglene, Stephen C.</span> and <span class="person_name">Morehead, David T.</span> and <span class="person_name">Cobcroft, J.M.</span> and <span class="person_name">Nichols, Peter D.</span> and <span class="person_name">Brown, M.R.</span> and <span class="person_name">Carson, Jeremy</span> (2006) <xhtml:em>Combined effects of feeding enriched rotifers and antibiotic addition on performance of striped trumpeter (Latris lineata) larvae.</xhtml:em> Aquaculture, 251 (2 - 4). pp. 456-471. ISSN 0044-8486</p><p style="margin-bottom: 1em" class="not_ep_block"></p><table style="margin-bottom: 1em" class="not_ep_block"><tr><td valign="top" style="text-align:center"><a href="http://eprints.utas.edu.au/2432/1/Battaglene_etal_antibiotiics.pdf"><img alt="[img]" src="http://eprints.utas.edu.au/style/images/fileicons/application_pdf.png" class="ep_doc_icon" border="0" /></a></td><td valign="top"><a href="http://eprints.utas.edu.au/2432/1/Battaglene_etal_antibiotiics.pdf"><span class="ep_document_citation">PDF</span></a> - Full text restricted - Requires a PDF viewer<br />205Kb</td><td><form method="get" accept-charset="utf-8" action="http://eprints.utas.edu.au/cgi/request_doc"><input accept-charset="utf-8" value="3172" name="docid" type="hidden" /><div class=""><input value="Request a copy" name="_action_null" class="ep_form_action_button" onclick="return EPJS_button_pushed( '_action_null' )" type="submit" /> </div></form></td></tr></table><p style="margin-bottom: 1em" class="not_ep_block">Official URL: <a href="http://dx.doi.org/10.1016/j.aquaculture.2005.06.021">http://dx.doi.org/10.1016/j.aquaculture.2005.06.021</a></p><div class="not_ep_block"><h2>Abstract</h2><p style="padding-bottom: 16px; text-align: left; margin: 1em auto 0em auto">Striped trumpeter have a complex and extended larval phase and are difficult to culture. Two experiments were&#13;
  460. conducted in replicated, 300-l hemispherical tanks to determine if larval survival, growth, bacterial or fatty acid profile&#13;
  461. were improved by feeding non-enriched rotifers or rotifers enriched with algae or commercially available products, as well&#13;
  462. as the effect of an antibiotic, oxytetracycline (OTC). Larvae were stocked at 25 l1 and 15 l1 and reared until Day 16&#13;
  463. and Day 19 in Experiments 1 and 2, respectively. In Experiment 1, the feeding treatments were non-enriched rotifers, or&#13;
  464. rotifers enriched on algae, DHA Selco (a fish oil based emulsion) or RotiMac (dried Schizochytrium). There were no&#13;
  465. significant differences in mean survival (FSD) across treatments, which were generally low at 14.6F5.2%. Larvae reared&#13;
  466. on rotifers fed DHA Selco and RotiMac, had significantly higher proportions of incorporated DHA but no significant&#13;
  467. increase in growth. In Experiment 2, larvae were fed rotifers enriched on DHA Selco or AlgaMac 2000 and reared with or&#13;
  468. without the daily addition of 25 mg l1 OTC. At Day 19, there was significantly higher survival for larvae reared on&#13;
  469. AlgaMac 2000 and OTC, (37.4F5.6%), than DHA Selco and OTC, (16.0F7.4%), AlgaMac 2000 without OTC&#13;
  470. (7.0F8.0) and DHA Selco without OTC (3.3F1.2). Larvae reared with OTC were larger (279F58 Ag and 7.4F0.2&#13;
  471. mm) than without OTC (177F40 Ag and 6.3F0.2 mm). The addition of antibiotics did not significantly influence fatty&#13;
  472. acid profiles of larvae. There were no significant differences in the percentage of DHA, 27.6F2.8%, EPA 4.6F1.0% or&#13;
  473. ARA 4.9F0.4%. Larvae reared with OTC had significantly less dgrey gutT (a measure of intestinal dysfunction). The&#13;
  474. results indicated that bacterial infection was a major source of mortality in striped trumpeter larvae and compromised larval&#13;
  475. growth. Assessment of the bacterial flora indicated that antibiotic use reduced the bacterial load, but did not eliminate</p></div><table style="margin-bottom: 1em" cellpadding="3" class="not_ep_block" border="0"><tr><th valign="top" class="ep_row">Item Type:</th><td valign="top" class="ep_row">Article</td></tr><tr><th valign="top" class="ep_row">Additional Information:</th><td valign="top" class="ep_row">The definitive version is available at http://www.sciencedirect.com&#13;
  476. </td></tr><tr><th valign="top" class="ep_row">Keywords:</th><td valign="top" class="ep_row">Latris lineata; Microheterotrophs; DHA; EPA; Antibiotic; Lipid; Marine fish larvae; Bacteria; Fatty acid</td></tr><tr><th valign="top" class="ep_row">Subjects:</th><td valign="top" class="ep_row">UNSPECIFIED</td></tr><tr><th valign="top" class="ep_row">ID Code:</th><td valign="top" class="ep_row">2432</td></tr><tr><th valign="top" class="ep_row">Deposited By:</th><td valign="top" class="ep_row"><span class="ep_name_citation"><span class="person_name">Scholarly Publications Librarian</span></span></td></tr><tr><th valign="top" class="ep_row">Deposited On:</th><td valign="top" class="ep_row">12 Nov 2007 11:55</td></tr><tr><th valign="top" class="ep_row">Last Modified:</th><td valign="top" class="ep_row">09 Jan 2008 02:30</td></tr><tr><th valign="top" class="ep_row">ePrint Statistics:</th><td valign="top" class="ep_row"><a target="ePrintStats" href="/es/index.php?action=show_detail_eprint;id=2432;">View statistics for this ePrint</a></td></tr></table><p align="right">Repository Staff Only: <a href="http://eprints.utas.edu.au/cgi/users/home?screen=EPrint::View&amp;eprintid=2432">item control page</a></p>
  477. </td></tr></table>
  478. </div>
  479.  
  480.  
  481.  
  482. <!-- InstanceEndEditable --></td>
  483. </tr>
  484. <tr>
  485. <td><!-- #BeginLibraryItem "/Library/footer_eprints.lbi" -->
  486. <table width="795" border="0" align="left" cellpadding="0" class="footer">
  487. <tr valign="top">
  488. <td colspan="2"><div align="center"><a href="http://www.utas.edu.au">UTAS home</a> | <a href="http://www.utas.edu.au/library/">Library home</a> | <a href="/">ePrints home</a> | <a href="/contact.html">contact</a> | <a href="/information.html">about</a> | <a href="/view/">browse</a> | <a href="/perl/search/simple">search</a> | <a href="/perl/register">register</a> | <a href="/perl/users/home">user area</a> | <a href="/help/">help</a></div><br /></td>
  489. </tr>
  490. <tr><td colspan="2"><p><img src="/images/eprints/footerline.gif" width="100%" height="4" /></p></td></tr>
  491. <tr valign="top">
  492. <td width="68%" class="footer">Authorised by the University Librarian<br />
  493. © University of Tasmania ABN 30 764 374 782<br />
  494. <a href="http://www.utas.edu.au/cricos/">CRICOS Provider Code 00586B</a> | <a href="http://www.utas.edu.au/copyright/copyright_disclaimers.html">Copyright &amp; Disclaimers</a> | <a href="http://www.utas.edu.au/accessibility/index.html">Accessibility</a> | <a href="http://eprints.utas.edu.au/feedback/">Site Feedback</a>  </td>
  495. <td width="32%"><div align="right">
  496. <p align="right" class="NoPrint"><a href="http://www.utas.edu.au/"><img src="http://www.utas.edu.au/shared/logos/unioftasstrip.gif" alt="University of Tasmania Home Page" width="260" height="16" border="0" align="right" /></a></p>
  497. <p align="right" class="NoPrint"><a href="http://www.utas.edu.au/"><br />
  498. </a></p>
  499. </div></td>
  500. </tr>
  501. <tr valign="top">
  502. <td><p>  </p></td>
  503. <td><div align="right"><span class="NoPrint"><a href="http://www.eprints.org/software/"><img src="/images/eprintslogo.gif" alt="ePrints logo" width="77" height="29" border="0" align="bottom" /></a></span></div></td>
  504. </tr>
  505. </table>
  506. <!-- #EndLibraryItem -->
  507. <div align="center"></div></td>
  508. </tr>
  509. </table>
  510.  
  511. </body>
  512. </html>