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<title>UTas ePrints - Combined effects of feeding enriched rotifers and antibiotic addition on performance of striped trumpeter (Latris lineata) larvae</title>
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<meta content="Nichols, Peter D." name="eprints.creators_name" />
<meta content="Brown, M.R." name="eprints.creators_name" />
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<meta content="Combined effects of feeding enriched rotifers and antibiotic
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<meta content="Latris lineata; Microheterotrophs; DHA; EPA; Antibiotic; Lipid; Marine fish larvae; Bacteria; Fatty acid" name="eprints.keywords" />
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<meta content="Striped trumpeter have a complex and extended larval phase and are difficult to culture. Two experiments were
conducted in replicated, 300-l hemispherical tanks to determine if larval survival, growth, bacterial or fatty acid profile
were improved by feeding non-enriched rotifers or rotifers enriched with algae or commercially available products, as well
as the effect of an antibiotic, oxytetracycline (OTC). Larvae were stocked at 25 l1 and 15 l1 and reared until Day 16
and Day 19 in Experiments 1 and 2, respectively. In Experiment 1, the feeding treatments were non-enriched rotifers, or
rotifers enriched on algae, DHA Selco (a fish oil based emulsion) or RotiMac (dried Schizochytrium). There were no
significant differences in mean survival (FSD) across treatments, which were generally low at 14.6F5.2%. Larvae reared
on rotifers fed DHA Selco and RotiMac, had significantly higher proportions of incorporated DHA but no significant
increase in growth. In Experiment 2, larvae were fed rotifers enriched on DHA Selco or AlgaMac 2000 and reared with or
without the daily addition of 25 mg l1 OTC. At Day 19, there was significantly higher survival for larvae reared on
AlgaMac 2000 and OTC, (37.4F5.6%), than DHA Selco and OTC, (16.0F7.4%), AlgaMac 2000 without OTC
(7.0F8.0) and DHA Selco without OTC (3.3F1.2). Larvae reared with OTC were larger (279F58 Ag and 7.4F0.2
mm) than without OTC (177F40 Ag and 6.3F0.2 mm). The addition of antibiotics did not significantly influence fatty
acid profiles of larvae. There were no significant differences in the percentage of DHA, 27.6F2.8%, EPA 4.6F1.0% or
ARA 4.9F0.4%. Larvae reared with OTC had significantly less dgrey gutT (a measure of intestinal dysfunction). The
results indicated that bacterial infection was a major source of mortality in striped trumpeter larvae and compromised larval
growth. Assessment of the bacterial flora indicated that antibiotic use reduced the bacterial load, but did not eliminate" name="eprints.abstract" />
<meta content="2006" name="eprints.date" />
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<meta content="Aquaculture" name="eprints.publication" />
<meta content="251" name="eprints.volume" />
<meta content="2 - 4" name="eprints.number" />
<meta content="456-471" name="eprints.pagerange" />
<meta content="10.1016/j.aquaculture.2005.06.021" name="eprints.id_number" />
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<meta content="Angulo, L., Lopez, J.E., Vicente, J.A., Saborido, A.M., 1994.
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<meta content="Battaglene, Stephen C. and Morehead, David T. and Cobcroft, J.M. and Nichols, Peter D. and Brown, M.R. and Carson, Jeremy (2006) Combined effects of feeding enriched rotifers and antibiotic addition on performance of striped trumpeter (Latris lineata) larvae. Aquaculture, 251 (2 - 4). pp. 456-471. ISSN 0044-8486" name="eprints.citation" />
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<meta content="Combined effects of feeding enriched rotifers and antibiotic
addition on performance of striped trumpeter (Latris lineata) larvae" name="DC.title" />
<meta content="Battaglene, Stephen C." name="DC.creator" />
<meta content="Morehead, David T." name="DC.creator" />
<meta content="Cobcroft, J.M." name="DC.creator" />
<meta content="Nichols, Peter D." name="DC.creator" />
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<meta content="Striped trumpeter have a complex and extended larval phase and are difficult to culture. Two experiments were
conducted in replicated, 300-l hemispherical tanks to determine if larval survival, growth, bacterial or fatty acid profile
were improved by feeding non-enriched rotifers or rotifers enriched with algae or commercially available products, as well
as the effect of an antibiotic, oxytetracycline (OTC). Larvae were stocked at 25 l1 and 15 l1 and reared until Day 16
and Day 19 in Experiments 1 and 2, respectively. In Experiment 1, the feeding treatments were non-enriched rotifers, or
rotifers enriched on algae, DHA Selco (a fish oil based emulsion) or RotiMac (dried Schizochytrium). There were no
significant differences in mean survival (FSD) across treatments, which were generally low at 14.6F5.2%. Larvae reared
on rotifers fed DHA Selco and RotiMac, had significantly higher proportions of incorporated DHA but no significant
increase in growth. In Experiment 2, larvae were fed rotifers enriched on DHA Selco or AlgaMac 2000 and reared with or
without the daily addition of 25 mg l1 OTC. At Day 19, there was significantly higher survival for larvae reared on
AlgaMac 2000 and OTC, (37.4F5.6%), than DHA Selco and OTC, (16.0F7.4%), AlgaMac 2000 without OTC
(7.0F8.0) and DHA Selco without OTC (3.3F1.2). Larvae reared with OTC were larger (279F58 Ag and 7.4F0.2
mm) than without OTC (177F40 Ag and 6.3F0.2 mm). The addition of antibiotics did not significantly influence fatty
acid profiles of larvae. There were no significant differences in the percentage of DHA, 27.6F2.8%, EPA 4.6F1.0% or
ARA 4.9F0.4%. Larvae reared with OTC had significantly less dgrey gutT (a measure of intestinal dysfunction). The
results indicated that bacterial infection was a major source of mortality in striped trumpeter larvae and compromised larval
growth. Assessment of the bacterial flora indicated that antibiotic use reduced the bacterial load, but did not eliminate" name="DC.description" />
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<h1 class="ep_tm_pagetitle">Combined effects of feeding enriched rotifers and antibiotic addition on performance of striped trumpeter (Latris lineata) larvae</h1>
<p style="margin-bottom: 1em" class="not_ep_block"><span class="person_name">Battaglene, Stephen C.</span> and <span class="person_name">Morehead, David T.</span> and <span class="person_name">Cobcroft, J.M.</span> and <span class="person_name">Nichols, Peter D.</span> and <span class="person_name">Brown, M.R.</span> and <span class="person_name">Carson, Jeremy</span> (2006) <xhtml:em>Combined effects of feeding enriched rotifers and antibiotic addition on performance of striped trumpeter (Latris lineata) larvae.</xhtml:em> Aquaculture, 251 (2 - 4). pp. 456-471. ISSN 0044-8486</p><p style="margin-bottom: 1em" class="not_ep_block"></p><table style="margin-bottom: 1em" class="not_ep_block"><tr><td valign="top" style="text-align:center"><a href="http://eprints.utas.edu.au/2432/1/Battaglene_etal_antibiotiics.pdf"><img alt="[img]" src="http://eprints.utas.edu.au/style/images/fileicons/application_pdf.png" class="ep_doc_icon" border="0" /></a></td><td valign="top"><a href="http://eprints.utas.edu.au/2432/1/Battaglene_etal_antibiotiics.pdf"><span class="ep_document_citation">PDF</span></a> - Full text restricted - Requires a PDF viewer<br />205Kb</td><td><form method="get" accept-charset="utf-8" action="http://eprints.utas.edu.au/cgi/request_doc"><input accept-charset="utf-8" value="3172" name="docid" type="hidden" /><div class=""><input value="Request a copy" name="_action_null" class="ep_form_action_button" onclick="return EPJS_button_pushed( '_action_null' )" type="submit" /> </div></form></td></tr></table><p style="margin-bottom: 1em" class="not_ep_block">Official URL: <a href="http://dx.doi.org/10.1016/j.aquaculture.2005.06.021">http://dx.doi.org/10.1016/j.aquaculture.2005.06.021</a></p><div class="not_ep_block"><h2>Abstract</h2><p style="padding-bottom: 16px; text-align: left; margin: 1em auto 0em auto">Striped trumpeter have a complex and extended larval phase and are difficult to culture. Two experiments were
conducted in replicated, 300-l hemispherical tanks to determine if larval survival, growth, bacterial or fatty acid profile
were improved by feeding non-enriched rotifers or rotifers enriched with algae or commercially available products, as well
as the effect of an antibiotic, oxytetracycline (OTC). Larvae were stocked at 25 l1 and 15 l1 and reared until Day 16
and Day 19 in Experiments 1 and 2, respectively. In Experiment 1, the feeding treatments were non-enriched rotifers, or
rotifers enriched on algae, DHA Selco (a fish oil based emulsion) or RotiMac (dried Schizochytrium). There were no
significant differences in mean survival (FSD) across treatments, which were generally low at 14.6F5.2%. Larvae reared
on rotifers fed DHA Selco and RotiMac, had significantly higher proportions of incorporated DHA but no significant
increase in growth. In Experiment 2, larvae were fed rotifers enriched on DHA Selco or AlgaMac 2000 and reared with or
without the daily addition of 25 mg l1 OTC. At Day 19, there was significantly higher survival for larvae reared on
AlgaMac 2000 and OTC, (37.4F5.6%), than DHA Selco and OTC, (16.0F7.4%), AlgaMac 2000 without OTC
(7.0F8.0) and DHA Selco without OTC (3.3F1.2). Larvae reared with OTC were larger (279F58 Ag and 7.4F0.2
mm) than without OTC (177F40 Ag and 6.3F0.2 mm). The addition of antibiotics did not significantly influence fatty
acid profiles of larvae. There were no significant differences in the percentage of DHA, 27.6F2.8%, EPA 4.6F1.0% or
ARA 4.9F0.4%. Larvae reared with OTC had significantly less dgrey gutT (a measure of intestinal dysfunction). The
results indicated that bacterial infection was a major source of mortality in striped trumpeter larvae and compromised larval
growth. Assessment of the bacterial flora indicated that antibiotic use reduced the bacterial load, but did not eliminate</p></div><table style="margin-bottom: 1em" cellpadding="3" class="not_ep_block" border="0"><tr><th valign="top" class="ep_row">Item Type:</th><td valign="top" class="ep_row">Article</td></tr><tr><th valign="top" class="ep_row">Additional Information:</th><td valign="top" class="ep_row">The definitive version is available at http://www.sciencedirect.com
</td></tr><tr><th valign="top" class="ep_row">Keywords:</th><td valign="top" class="ep_row">Latris lineata; Microheterotrophs; DHA; EPA; Antibiotic; Lipid; Marine fish larvae; Bacteria; Fatty acid</td></tr><tr><th valign="top" class="ep_row">Subjects:</th><td valign="top" class="ep_row">UNSPECIFIED</td></tr><tr><th valign="top" class="ep_row">ID Code:</th><td valign="top" class="ep_row">2432</td></tr><tr><th valign="top" class="ep_row">Deposited By:</th><td valign="top" class="ep_row"><span class="ep_name_citation"><span class="person_name">Scholarly Publications Librarian</span></span></td></tr><tr><th valign="top" class="ep_row">Deposited On:</th><td valign="top" class="ep_row">12 Nov 2007 11:55</td></tr><tr><th valign="top" class="ep_row">Last Modified:</th><td valign="top" class="ep_row">09 Jan 2008 02:30</td></tr><tr><th valign="top" class="ep_row">ePrint Statistics:</th><td valign="top" class="ep_row"><a target="ePrintStats" href="/es/index.php?action=show_detail_eprint;id=2432;">View statistics for this ePrint</a></td></tr></table><p align="right">Repository Staff Only: <a href="http://eprints.utas.edu.au/cgi/users/home?screen=EPrint::View&eprintid=2432">item control page</a></p>
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