<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" "http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd"> <html> <head> <title>UTas ePrints - Genetic diversity and breed comparison of carcass traits in Tasmanian Corriedale and East-Friesian sheep by RAPD markers</title> <script type="text/javascript" src="http://eprints.utas.edu.au/javascript/auto.js"><!-- padder --></script> <style type="text/css" media="screen">@import url(http://eprints.utas.edu.au/style/auto.css);</style> <style type="text/css" media="print">@import url(http://eprints.utas.edu.au/style/print.css);</style> <link rel="icon" href="/images/eprints/favicon.ico" type="image/x-icon" /> <link rel="shortcut icon" href="/images/eprints/favicon.ico" type="image/x-icon" /> <link rel="Top" href="http://eprints.utas.edu.au/" /> <link rel="Search" href="http://eprints.utas.edu.au/cgi/search" /> <meta content="Malau-Aduli, A.E.O." name="eprints.creators_name" /> <meta content="Bignell, C.W." name="eprints.creators_name" /> <meta content="Tavassoli-Salardini, F." name="eprints.creators_name" /> <meta content="Smolenski, A.J." name="eprints.creators_name" /> <meta content="Palmer, A." name="eprints.creators_name" /> <meta content="Bignell, J." name="eprints.creators_name" /> <meta content="Burbury, S." name="eprints.creators_name" /> <meta content="Batchelor, R." name="eprints.creators_name" /> <meta content="Malau-Aduli, B.S." name="eprints.creators_name" /> <meta content="Adediran, S.A." name="eprints.creators_name" /> <meta content="Lane, P.A." name="eprints.creators_name" /> <meta content="Clark, R.J." name="eprints.creators_name" /> <meta content="Aduli.MalauAduli@utas.edu.au" name="eprints.creators_id" /> <meta content="" name="eprints.creators_id" /> <meta content="" name="eprints.creators_id" /> <meta content="" name="eprints.creators_id" /> <meta content="" name="eprints.creators_id" /> <meta content="" name="eprints.creators_id" /> <meta content="" name="eprints.creators_id" /> <meta content="" name="eprints.creators_id" /> <meta content="" name="eprints.creators_id" /> <meta content="" name="eprints.creators_id" /> <meta content="" name="eprints.creators_id" /> <meta content="" name="eprints.creators_id" /> <meta content="Troy, Declan" name="eprints.editors_name" /> <meta content="Pearce, Rachel" name="eprints.editors_name" /> <meta content="Byrne, Briege" name="eprints.editors_name" /> <meta content="Kerry, Joseph" name="eprints.editors_name" /> <meta content="book_section" name="eprints.type" /> <meta content="2008-01-18T03:34:31Z" name="eprints.datestamp" /> <meta content="2008-01-18T03:34:31Z" name="eprints.lastmod" /> <meta content="show" name="eprints.metadata_visibility" /> <meta content="Genetic diversity and breed comparison of carcass traits in Tasmanian Corriedale and East-Friesian sheep by RAPD markers" name="eprints.title" /> <meta content="pub" name="eprints.ispublished" /> <meta content="270200" name="eprints.subjects" /> <meta content="300401" name="eprints.subjects" /> <meta content="270208" name="eprints.subjects" /> <meta content="300400" name="eprints.subjects" /> <meta content="270800" name="eprints.subjects" /> <meta content="restricted" name="eprints.full_text_status" /> <meta content="RAPD markers, Corriedale, East Friesian, meat quality, genetic diversity" name="eprints.keywords" /> <meta content="RAPD marker assays are based on polymerase chain reaction amplification of random segments of the DNA with an identical pair of primers 8-10 bp in length consisting of arbitrary nucleotide sequence. Genetic variation and divergence within and between breeds of interest are assessed by the presence or absence of each product which is dictated by the DNA sequence at each locus. The power to detect polymorphisms is very high given that 5-20 bands can be produced using a given primer pair and multiple sets of random primers can be used to scan the entire genome for differential RAPD bands. RAPD has several advantages over other molecular markers because it can be used with uncharacterised genomes without prior knowledge of nucleotide sequence information and can be applied to problems in which only small quantities of DNA are available. It is also efficient and inexpensive.At the same slaughter weight and body condition score, Corriedales had significantly (P<0.01) higher fat score, thicker subcutaneous fat (29.6 vs 23.3 mm) and wider eye muscle (49.8 vs 36.2 mm) than East Friesians. On the other hand, East Friesians had significantly (P<0.01) heavier hot carcass weight (23.8 vs 22.3 kg), larger eye muscle area (42.9 vs 39.5 cm2) and longer eye muscle (67.1 vs 65.6 mm) than Corriedales, while shortloin length (SLL) in the two breeds did not significantly differ (P>0.09). " name="eprints.abstract" /> <meta content="2007-08-13" name="eprints.date" /> <meta content="published" name="eprints.date_type" /> <meta content="In: D. Troy, R. Pearce, B. Byrne, J. Kerry (Editors), Harnessing and Exploiting Global Opportunities’, Proceedings of the 52nd International Congress of Meat Science and Technology, University College Dublin, Ireland." name="eprints.publication" /> <meta content="52" name="eprints.volume" /> <meta content="Wageningen Academic Publishers" name="eprints.publisher" /> <meta content="75-76" name="eprints.pagerange" /> <meta content="765" name="eprints.pages" /> <meta content="FALSE" name="eprints.refereed" /> <meta content="978-90-8686-010-4" name="eprints.isbn" /> <meta content="978908680104" name="eprints.issn" /> <meta content="52nd International Congress of Meat Science and Technology" name="eprints.book_title" /> <meta content="http://www.wageningenacademic.com/default.asp?pageid=8&docid=16&artdetail=Meatscience&webgroupfilter=2&" name="eprints.official_url" /> <meta content="1. Fogarty, N. M., Hopkins, D. L., van de Ven, R. (2000). Lamb production from diverse genotypes 2. Carcass characteristics. Animal Science, 70: 147-156. 2. Fogarty, N. M., Safari, E., Taylor, P. J., Murray, W. (2003). Genetic parameters for meat quality and carcass traits and their correlation with wool traits in Australian Merino sheep. Australian Journal of Agricultural Research, 54: 715-722. 3. Fogarty, N. M., Ingham, V. M., Gilmour, A. R., Cummins, L. J., Gaunt, G. M., Stafford, J., Hocking-Edwards, J. E., Banks, R. G. (2005a). Genetic evaluation of crossbred lamb production 1. Breed and fixed effects for birth and weaning weight of first-cross lambs, gestation length and reproduction of base ewes. Australian Journal of Agricultural Research, 56: 443-453. 4. Fogarty, N. M., Ingham, V. M., Gilmour, A. R., Cummins, L. J., Gaunt, G. M., Stafford, J., Hocking-Edwards, J. E., Banks, R. G. (2005b). Genetic evaluation of crossbred lamb production 2. Breed and fixed effects for post-weaning growth, carcass and wool of first-cross lambs. Australian Journal of Agricultural Research, 56: 455-463. 5.Liu, Z. J., Cordes, J. F. (2004). DNA marker technologies and their applications in aquaculture genetics. Aquaculture, 238: 1-37. 6.Malau-Aduli, A. E. O., Bignell, C. W., Hegarty, R. S., Oddy, H., Johns, W., Tavassoli-Salardini, F., Smolenski, A. J., Malau-Aduli, B. S., Wells, B. B., Lane, P. A., Clark, R. J. (2006). Random amplified polymorphic DNA marker prospects for detecting genetic variation in growth and meat quality of crossbred lambs sired by rams with high EBVs for muscle or growth fed high or low levels of nutrition. Australian Journal of Experimental Agriculture, Volume 46: In press 7. Rabouam, C., Comes, A. M., Bretagnolle, V., Humbert, J. F., Periquet, G., Bigot, Y. (1999). Features of DNA fragments obtained by random amplified polymorphic DNA (RAPD) assays. Molecular Ecology, 8: 493-503. " name="eprints.referencetext" /> <meta content="Malau-Aduli, A.E.O. and Bignell, C.W. and Tavassoli-Salardini, F. and Smolenski, A.J. and Palmer, A. and Bignell, J. and Burbury, S. and Batchelor, R. and Malau-Aduli, B.S. and Adediran, S.A. and Lane, P.A. and Clark, R.J. (2007) Genetic diversity and breed comparison of carcass traits in Tasmanian Corriedale and East-Friesian sheep by RAPD markers. In: 52nd International Congress of Meat Science and Technology. . Wageningen Academic Publishers, pp. 75-76. ISBN 978-90-8686-010-4" name="eprints.citation" /> <meta content="http://eprints.utas.edu.au/2970/2/MALAU-ADULI_et_al_2006DublinTassieSheep.pdf" name="eprints.document_url" /> <link rel="schema.DC" href="http://purl.org/DC/elements/1.0/" /> <meta content="Genetic diversity and breed comparison of carcass traits in Tasmanian Corriedale and East-Friesian sheep by RAPD markers" name="DC.title" /> <meta content="Malau-Aduli, A.E.O." name="DC.creator" /> <meta content="Bignell, C.W." name="DC.creator" /> <meta content="Tavassoli-Salardini, F." name="DC.creator" /> <meta content="Smolenski, A.J." name="DC.creator" /> <meta content="Palmer, A." name="DC.creator" /> <meta content="Bignell, J." name="DC.creator" /> <meta content="Burbury, S." name="DC.creator" /> <meta content="Batchelor, R." name="DC.creator" /> <meta content="Malau-Aduli, B.S." name="DC.creator" /> <meta content="Adediran, S.A." name="DC.creator" /> <meta content="Lane, P.A." name="DC.creator" /> <meta content="Clark, R.J." name="DC.creator" /> <meta content="270200 Genetics" name="DC.subject" /> <meta content="300401 Animal Breeding" name="DC.subject" /> <meta content="270208 Molecular Evolution" name="DC.subject" /> <meta content="300400 Animal Production" name="DC.subject" /> <meta content="270800 Biotechnology" name="DC.subject" /> <meta content="RAPD marker assays are based on polymerase chain reaction amplification of random segments of the DNA with an identical pair of primers 8-10 bp in length consisting of arbitrary nucleotide sequence. Genetic variation and divergence within and between breeds of interest are assessed by the presence or absence of each product which is dictated by the DNA sequence at each locus. The power to detect polymorphisms is very high given that 5-20 bands can be produced using a given primer pair and multiple sets of random primers can be used to scan the entire genome for differential RAPD bands. RAPD has several advantages over other molecular markers because it can be used with uncharacterised genomes without prior knowledge of nucleotide sequence information and can be applied to problems in which only small quantities of DNA are available. It is also efficient and inexpensive.At the same slaughter weight and body condition score, Corriedales had significantly (P<0.01) higher fat score, thicker subcutaneous fat (29.6 vs 23.3 mm) and wider eye muscle (49.8 vs 36.2 mm) than East Friesians. On the other hand, East Friesians had significantly (P<0.01) heavier hot carcass weight (23.8 vs 22.3 kg), larger eye muscle area (42.9 vs 39.5 cm2) and longer eye muscle (67.1 vs 65.6 mm) than Corriedales, while shortloin length (SLL) in the two breeds did not significantly differ (P>0.09). " name="DC.description" /> <meta content="Wageningen Academic Publishers" name="DC.publisher" /> <meta content="Troy, Declan" name="DC.contributor" /> <meta content="Pearce, Rachel" name="DC.contributor" /> <meta content="Byrne, Briege" name="DC.contributor" /> <meta content="Kerry, Joseph" name="DC.contributor" /> <meta content="2007-08-13" name="DC.date" /> <meta content="Book Chapter" name="DC.type" /> <meta content="NonPeerReviewed" name="DC.type" /> <meta content="application/pdf" name="DC.format" /> <meta content="http://eprints.utas.edu.au/2970/2/MALAU-ADULI_et_al_2006DublinTassieSheep.pdf" name="DC.identifier" /> <meta content="http://www.wageningenacademic.com/default.asp?pageid=8&docid=16&artdetail=Meatscience&webgroupfilter=2&" name="DC.relation" /> <meta content="Malau-Aduli, A.E.O. and Bignell, C.W. and Tavassoli-Salardini, F. and Smolenski, A.J. and Palmer, A. and Bignell, J. and Burbury, S. and Batchelor, R. and Malau-Aduli, B.S. and Adediran, S.A. and Lane, P.A. and Clark, R.J. (2007) Genetic diversity and breed comparison of carcass traits in Tasmanian Corriedale and East-Friesian sheep by RAPD markers. In: 52nd International Congress of Meat Science and Technology. . Wageningen Academic Publishers, pp. 75-76. 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border: solid 1px #ccc; padding: 3px"><tr> <td align="left"><a href="http://eprints.utas.edu.au/cgi/users/home">Login</a> | <a href="http://eprints.utas.edu.au/cgi/register">Create Account</a></td> <td align="right" style="white-space: nowrap"> <form method="get" accept-charset="utf-8" action="http://eprints.utas.edu.au/cgi/search" style="display:inline"> <input class="ep_tm_searchbarbox" size="20" type="text" name="q" /> <input class="ep_tm_searchbarbutton" value="Search" type="submit" name="_action_search" /> <input type="hidden" name="_order" value="bytitle" /> <input type="hidden" name="basic_srchtype" value="ALL" /> <input type="hidden" name="_satisfyall" value="ALL" /> </form> </td> </tr></table></td></tr> <tr> <td class="toplinks"><!-- InstanceBeginEditable name="content" --> <div align="center"> <table width="720" class="ep_tm_main"><tr><td align="left"> <h1 class="ep_tm_pagetitle">Genetic diversity and breed comparison of carcass traits in Tasmanian Corriedale and East-Friesian sheep by RAPD markers</h1> <p style="margin-bottom: 1em" class="not_ep_block"><span class="person_name">Malau-Aduli, A.E.O.</span> and <span class="person_name">Bignell, C.W.</span> and <span class="person_name">Tavassoli-Salardini, F.</span> and <span class="person_name">Smolenski, A.J.</span> and <span class="person_name">Palmer, A.</span> and <span class="person_name">Bignell, J.</span> and <span class="person_name">Burbury, S.</span> and <span class="person_name">Batchelor, R.</span> and <span class="person_name">Malau-Aduli, B.S.</span> and <span class="person_name">Adediran, S.A.</span> and <span class="person_name">Lane, P.A.</span> and <span class="person_name">Clark, R.J.</span> (2007) <xhtml:em>Genetic diversity and breed comparison of carcass traits in Tasmanian Corriedale and East-Friesian sheep by RAPD markers.</xhtml:em> In: 52nd International Congress of Meat Science and Technology. . Wageningen Academic Publishers, pp. 75-76. ISBN 978-90-8686-010-4</p><p style="margin-bottom: 1em" class="not_ep_block"></p><table style="margin-bottom: 1em" class="not_ep_block"><tr><td valign="top" style="text-align:center"><a href="http://eprints.utas.edu.au/2970/2/MALAU-ADULI_et_al_2006DublinTassieSheep.pdf"><img alt="[img]" src="http://eprints.utas.edu.au/style/images/fileicons/application_pdf.png" border="0" class="ep_doc_icon" /></a></td><td valign="top"><a href="http://eprints.utas.edu.au/2970/2/MALAU-ADULI_et_al_2006DublinTassieSheep.pdf"><span class="ep_document_citation">PDF</span></a> - Full text restricted - Requires a PDF viewer<br />153Kb</td><td><form method="get" accept-charset="utf-8" action="http://eprints.utas.edu.au/cgi/request_doc"><input value="3996" name="docid" accept-charset="utf-8" type="hidden" /><div class=""><input value="Request a copy" name="_action_null" class="ep_form_action_button" onclick="return EPJS_button_pushed( '_action_null' )" type="submit" /> </div></form></td></tr></table><p style="margin-bottom: 1em" class="not_ep_block">Official URL: <a href="http://www.wageningenacademic.com/default.asp?pageid=8&docid=16&artdetail=Meatscience&webgroupfilter=2&">http://www.wageningenacademic.com/default.asp?pageid=8&docid=16&artdetail=Meatscience&webgroupfilter=2&</a></p><div class="not_ep_block"><h2>Abstract</h2><p style="padding-bottom: 16px; text-align: left; margin: 1em auto 0em auto">RAPD marker assays are based on polymerase chain reaction amplification of random segments of the DNA with an identical pair of primers 8-10 bp in length consisting of arbitrary nucleotide sequence. Genetic variation and divergence within and between breeds of interest are assessed by the presence or absence of each product which is dictated by the DNA sequence at each locus. The power to detect polymorphisms is very high given that 5-20 bands can be produced using a given primer pair and multiple sets of random primers can be used to scan the entire genome for differential RAPD bands. RAPD has several advantages over other molecular markers because it can be used with uncharacterised genomes without prior knowledge of nucleotide sequence information and can be applied to problems in which only small quantities of DNA are available. It is also efficient and inexpensive.At the same slaughter weight and body condition score, Corriedales had significantly (P<0.01) higher fat score, thicker subcutaneous fat (29.6 vs 23.3 mm) and wider eye muscle (49.8 vs 36.2 mm) than East Friesians. On the other hand, East Friesians had significantly (P<0.01) heavier hot carcass weight (23.8 vs 22.3 kg), larger eye muscle area (42.9 vs 39.5 cm2) and longer eye muscle (67.1 vs 65.6 mm) than Corriedales, while shortloin length (SLL) in the two breeds did not significantly differ (P>0.09). </p></div><table style="margin-bottom: 1em" border="0" cellpadding="3" class="not_ep_block"><tr><th valign="top" class="ep_row">Item Type:</th><td valign="top" class="ep_row">Book Chapter</td></tr><tr><th valign="top" class="ep_row">Keywords:</th><td valign="top" class="ep_row">RAPD markers, Corriedale, East Friesian, meat quality, genetic diversity</td></tr><tr><th valign="top" class="ep_row">Subjects:</th><td valign="top" class="ep_row"><a href="http://eprints.utas.edu.au/view/subjects/270200.html">270000 Biological Sciences > 270200 Genetics</a><br /><a href="http://eprints.utas.edu.au/view/subjects/300401.html">300000 Agricultural, Veterinary and Environmental Sciences > 300400 Animal Production > 300401 Animal Breeding</a><br /><a href="http://eprints.utas.edu.au/view/subjects/270208.html">270000 Biological Sciences > 270200 Genetics > 270208 Molecular Evolution</a><br /><a href="http://eprints.utas.edu.au/view/subjects/300400.html">300000 Agricultural, Veterinary and Environmental Sciences > 300400 Animal Production</a><br /><a href="http://eprints.utas.edu.au/view/subjects/270800.html">270000 Biological Sciences > 270800 Biotechnology</a></td></tr><tr><th valign="top" class="ep_row">Collections:</th><td valign="top" class="ep_row">UNSPECIFIED</td></tr><tr><th valign="top" class="ep_row">ID Code:</th><td valign="top" class="ep_row">2970</td></tr><tr><th valign="top" class="ep_row">Deposited By:</th><td valign="top" class="ep_row"><span class="ep_name_citation"><span class="person_name">Dr Aduli E.O. Malau-Aduli</span></span></td></tr><tr><th valign="top" class="ep_row">Deposited On:</th><td valign="top" class="ep_row">18 Jan 2008 14:34</td></tr><tr><th valign="top" class="ep_row">Last Modified:</th><td valign="top" class="ep_row">18 Jan 2008 14:34</td></tr><tr><th valign="top" class="ep_row">ePrint Statistics:</th><td valign="top" class="ep_row"><a target="ePrintStats" href="/es/index.php?action=show_detail_eprint;id=2970;">View statistics for this ePrint</a></td></tr></table><p align="right">Repository Staff Only: <a href="http://eprints.utas.edu.au/cgi/users/home?screen=EPrint::View&eprintid=2970">item control page</a></p> </td></tr></table> </div> <!-- InstanceEndEditable --></td> </tr> <tr> <td><!-- #BeginLibraryItem "/Library/footer_eprints.lbi" --> <table width="795" border="0" align="left" cellpadding="0" class="footer"> <tr valign="top"> <td colspan="2"><div align="center"><a href="http://www.utas.edu.au">UTAS home</a> | <a href="http://www.utas.edu.au/library/">Library home</a> | <a href="/">ePrints home</a> | <a href="/contact.html">contact</a> | <a href="/information.html">about</a> | <a href="/view/">browse</a> | <a href="/perl/search/simple">search</a> | <a href="/perl/register">register</a> | <a href="/perl/users/home">user area</a> | <a href="/help/">help</a></div><br /></td> </tr> <tr><td colspan="2"><p><img src="/images/eprints/footerline.gif" width="100%" height="4" /></p></td></tr> <tr valign="top"> <td width="68%" class="footer">Authorised by the University Librarian<br /> © University of Tasmania ABN 30 764 374 782<br /> <a href="http://www.utas.edu.au/cricos/">CRICOS Provider Code 00586B</a> | <a href="http://www.utas.edu.au/copyright/copyright_disclaimers.html">Copyright & Disclaimers</a> | <a href="http://www.utas.edu.au/accessibility/index.html">Accessibility</a> | <a href="http://eprints.utas.edu.au/feedback/">Site Feedback</a> </td> <td width="32%"><div align="right"> <p align="right" class="NoPrint"><a href="http://www.utas.edu.au/"><img src="http://www.utas.edu.au/shared/logos/unioftasstrip.gif" alt="University of Tasmania Home Page" width="260" height="16" border="0" align="right" /></a></p> <p align="right" class="NoPrint"><a href="http://www.utas.edu.au/"><br /> </a></p> </div></td> </tr> <tr valign="top"> <td><p> </p></td> <td><div align="right"><span class="NoPrint"><a href="http://www.eprints.org/software/"><img src="/images/eprintslogo.gif" alt="ePrints logo" width="77" height="29" border="0" align="bottom" /></a></span></div></td> </tr> </table> <!-- #EndLibraryItem --> <div align="center"></div></td> </tr> </table> </body> </html>