<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" "http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd"> <html> <head> <title>UTas ePrints - RAPD marker variation in meat quality traits of Poll Dorset second-cross lambs selected for muscle or growth</title> <script type="text/javascript" src="http://eprints.utas.edu.au/javascript/auto.js"><!-- padder --></script> <style type="text/css" media="screen">@import url(http://eprints.utas.edu.au/style/auto.css);</style> <style type="text/css" media="print">@import url(http://eprints.utas.edu.au/style/print.css);</style> <link rel="icon" href="/images/eprints/favicon.ico" type="image/x-icon" /> <link rel="shortcut icon" href="/images/eprints/favicon.ico" type="image/x-icon" /> <link rel="Top" href="http://eprints.utas.edu.au/" /> <link rel="Search" href="http://eprints.utas.edu.au/cgi/search" /> <meta content="Malau-Aduli, A.E.O." name="eprints.creators_name" /> <meta content="Bignell, C.W." name="eprints.creators_name" /> <meta content="Hegarty, R.S." name="eprints.creators_name" /> <meta content="Oddy, H." name="eprints.creators_name" /> <meta content="Johns, W." name="eprints.creators_name" /> <meta content="Tavassoli-Salardini, F." name="eprints.creators_name" /> <meta content="Smolenski, A.J." name="eprints.creators_name" /> <meta content="Malau-Aduli, B.S." name="eprints.creators_name" /> <meta content="Wells, B.B." name="eprints.creators_name" /> <meta content="Lane, P.A." name="eprints.creators_name" /> <meta content="Clark, R.J." name="eprints.creators_name" /> <meta content="Aduli.MalauAduli@utas.edu.au" name="eprints.creators_id" /> <meta content="" name="eprints.creators_id" /> <meta content="" name="eprints.creators_id" /> <meta content="" name="eprints.creators_id" /> <meta content="" name="eprints.creators_id" /> <meta content="" name="eprints.creators_id" /> <meta content="" name="eprints.creators_id" /> <meta content="" name="eprints.creators_id" /> <meta content="" name="eprints.creators_id" /> <meta content="" name="eprints.creators_id" /> <meta content="" name="eprints.creators_id" /> <meta content="conference_item" name="eprints.type" /> <meta content="2008-01-24T01:10:07Z" name="eprints.datestamp" /> <meta content="2008-01-24T01:10:07Z" name="eprints.lastmod" /> <meta content="show" name="eprints.metadata_visibility" /> <meta content="RAPD marker variation in meat quality traits of Poll Dorset second-cross lambs selected for muscle or growth " name="eprints.title" /> <meta content="pub" name="eprints.ispublished" /> <meta content="270200" name="eprints.subjects" /> <meta content="300401" name="eprints.subjects" /> <meta content="270208" name="eprints.subjects" /> <meta content="300400" name="eprints.subjects" /> <meta content="270800" name="eprints.subjects" /> <meta content="public" name="eprints.full_text_status" /> <meta content="paper" name="eprints.pres_type" /> <meta content="RAPD markers, Poll Dorset crossbred lambs, meat quality, growth, muscle" name="eprints.keywords" /> <meta content="In: D. Troy, R. Pearce, B. Byrne, J. Kerry (Editors), Harnessing and Exploiting Global Opportunities’, Proceedings of the 52nd International Congress of Meat Science and Technology, University College Dublin, Ireland, 52:89-90 (2006)" name="eprints.note" /> <meta content="All organisms are subject to mutations as a result of normal cellular operations or interactions with the environment, leading to genetic variation (polymorphism). In conjunction with selection and genetic drift, there arises genetic variation within and among individuals and species. For this variation to be useful to geneticists, it must be heritable and discernable whether as a recognisable phenotypic variation or as a genetic mutation distinguishable through molecular techniques. PCR amplification and gel electrophoresis resolution of products using RAPD primer A2 shows polymorphism of up to 5 bands was evident. It also demonstrates that at the molecular level, there are banding differences that can be picked up between crossbred progeny sired by rams with high genetic merit for growth or muscle.Fat depth at the GR and C sites was significantly (P<0.05) influenced by level of nutrition and nutrition level x siretype interaction in that fat depths were greater in crossbred lambs fed at high levels of nutrition than those fed low nutrition levels. Lambs selected for growth and fed high level of nutrition produced carcasses with the least KNIFE GR fat depth of 11.4mm, a significant reduction from 18.1mm in the control group fed low level nutrition. " name="eprints.abstract" /> <meta content="2006-08-13" name="eprints.date" /> <meta content="published" name="eprints.date_type" /> <meta content="In: D. Troy, R. Pearce, B. Byrne, J. Kerry (Editors), 'Harnessing and Exploiting Global Opportunities’, Proceedings of the 52nd International Congress of Meat Science and Technology, University College Dublin, Ireland." name="eprints.publication" /> <meta content="52" name="eprints.volume" /> <meta content="89-90" name="eprints.pagerange" /> <meta content="52nd International Congress of Meat Science and Technology" name="eprints.event_title" /> <meta content="Dublin, Ireland" name="eprints.event_location" /> <meta content="13 - 18 August 2006" name="eprints.event_dates" /> <meta content="conference" name="eprints.event_type" /> <meta content="TRUE" name="eprints.refereed" /> <meta content="978-90-8686-010-4" name="eprints.issn" /> <meta content="http://www.cpregistrations.com/icomst2006/" name="eprints.official_url" /> <meta content="1. Hegarty, R. S., Shands, C., Marchant, R., Hopkins, D. L., Ball, A., Harden, S. (2006). Effects of available nutrition and sire breeding values for growth and muscling on the development of crossbred lambs 1. Growth and carcass characteristics. Australian Journal of Agricultural Research in press. 2. Liu, Z. J., Cordes, J. F. (2004). DNA marker technologies and their applications in aquaculture genetics. Aquaculture, 238: 1-37. 3. Malau-Aduli, A. E. O., Bignell, C.W., Tavassoli-Salardini, F., Smolenski, A. J., Palmer, A., Bignell, J., Burbury, S., Batchelor, R., Malau-Aduli, B. S., Adediran, S. A., Lane, P. A., Clark, R. J. (2006). Genetic diversity and breed comparison of carcass traits in Tasmanian Corriedale and East Friesian sheep by RAPD markers. Proceedings of the 52nd International Congress on Meat Science and Technology, University College Dublin, Ireland. 4. Rabouam, C., Comes, A. M., Bretagnolle, V., Humbert, J. F., Periquet, G., Bigot, Y. (1999). Features of DNA fragments obtained by random amplified polymorphic DNA (RAPD) assays. Molecular Ecology, 8: 493-503. 5. Sambrook, J., Russell, D. W. (2001). Molecular Cloning: A laboratory manual, Cold Spring Harbor Laboratory Press, New York. " name="eprints.referencetext" /> <meta content="Malau-Aduli, A.E.O. and Bignell, C.W. and Hegarty, R.S. and Oddy, H. and Johns, W. and Tavassoli-Salardini, F. and Smolenski, A.J. and Malau-Aduli, B.S. and Wells, B.B. and Lane, P.A. and Clark, R.J. (2006) RAPD marker variation in meat quality traits of Poll Dorset second-cross lambs selected for muscle or growth. In: 52nd International Congress of Meat Science and Technology, 13 - 18 August 2006, Dublin, Ireland." name="eprints.citation" /> <meta content="http://eprints.utas.edu.au/2971/1/MALAU-ADULI_et_al_2006DublinPollDorsetSheep.pdf" name="eprints.document_url" /> <link rel="schema.DC" href="http://purl.org/DC/elements/1.0/" /> <meta content="RAPD marker variation in meat quality traits of Poll Dorset second-cross lambs selected for muscle or growth " name="DC.title" /> <meta content="Malau-Aduli, A.E.O." name="DC.creator" /> <meta content="Bignell, C.W." name="DC.creator" /> <meta content="Hegarty, R.S." name="DC.creator" /> <meta content="Oddy, H." name="DC.creator" /> <meta content="Johns, W." name="DC.creator" /> <meta content="Tavassoli-Salardini, F." name="DC.creator" /> <meta content="Smolenski, A.J." name="DC.creator" /> <meta content="Malau-Aduli, B.S." name="DC.creator" /> <meta content="Wells, B.B." name="DC.creator" /> <meta content="Lane, P.A." name="DC.creator" /> <meta content="Clark, R.J." name="DC.creator" /> <meta content="270200 Genetics" name="DC.subject" /> <meta content="300401 Animal Breeding" name="DC.subject" /> <meta content="270208 Molecular Evolution" name="DC.subject" /> <meta content="300400 Animal Production" name="DC.subject" /> <meta content="270800 Biotechnology" name="DC.subject" /> <meta content="All organisms are subject to mutations as a result of normal cellular operations or interactions with the environment, leading to genetic variation (polymorphism). In conjunction with selection and genetic drift, there arises genetic variation within and among individuals and species. For this variation to be useful to geneticists, it must be heritable and discernable whether as a recognisable phenotypic variation or as a genetic mutation distinguishable through molecular techniques. PCR amplification and gel electrophoresis resolution of products using RAPD primer A2 shows polymorphism of up to 5 bands was evident. It also demonstrates that at the molecular level, there are banding differences that can be picked up between crossbred progeny sired by rams with high genetic merit for growth or muscle.Fat depth at the GR and C sites was significantly (P<0.05) influenced by level of nutrition and nutrition level x siretype interaction in that fat depths were greater in crossbred lambs fed at high levels of nutrition than those fed low nutrition levels. Lambs selected for growth and fed high level of nutrition produced carcasses with the least KNIFE GR fat depth of 11.4mm, a significant reduction from 18.1mm in the control group fed low level nutrition. " name="DC.description" /> <meta content="2006-08-13" name="DC.date" /> <meta content="Conference or Workshop Item" name="DC.type" /> <meta content="PeerReviewed" name="DC.type" /> <meta content="application/pdf" name="DC.format" /> <meta content="http://eprints.utas.edu.au/2971/1/MALAU-ADULI_et_al_2006DublinPollDorsetSheep.pdf" name="DC.identifier" /> <meta content="http://www.cpregistrations.com/icomst2006/" name="DC.relation" /> <meta content="Malau-Aduli, A.E.O. and Bignell, C.W. and Hegarty, R.S. and Oddy, H. and Johns, W. and Tavassoli-Salardini, F. and Smolenski, A.J. and Malau-Aduli, B.S. and Wells, B.B. and Lane, P.A. and Clark, R.J. (2006) RAPD marker variation in meat quality traits of Poll Dorset second-cross lambs selected for muscle or growth. 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border: solid 1px #ccc; padding: 3px"><tr> <td align="left"><a href="http://eprints.utas.edu.au/cgi/users/home">Login</a> | <a href="http://eprints.utas.edu.au/cgi/register">Create Account</a></td> <td align="right" style="white-space: nowrap"> <form method="get" accept-charset="utf-8" action="http://eprints.utas.edu.au/cgi/search" style="display:inline"> <input class="ep_tm_searchbarbox" size="20" type="text" name="q" /> <input class="ep_tm_searchbarbutton" value="Search" type="submit" name="_action_search" /> <input type="hidden" name="_order" value="bytitle" /> <input type="hidden" name="basic_srchtype" value="ALL" /> <input type="hidden" name="_satisfyall" value="ALL" /> </form> </td> </tr></table></td></tr> <tr> <td class="toplinks"><!-- InstanceBeginEditable name="content" --> <div align="center"> <table width="720" class="ep_tm_main"><tr><td align="left"> <h1 class="ep_tm_pagetitle">RAPD marker variation in meat quality traits of Poll Dorset second-cross lambs selected for muscle or growth</h1> <p style="margin-bottom: 1em" class="not_ep_block"><span class="person_name">Malau-Aduli, A.E.O.</span> and <span class="person_name">Bignell, C.W.</span> and <span class="person_name">Hegarty, R.S.</span> and <span class="person_name">Oddy, H.</span> and <span class="person_name">Johns, W.</span> and <span class="person_name">Tavassoli-Salardini, F.</span> and <span class="person_name">Smolenski, A.J.</span> and <span class="person_name">Malau-Aduli, B.S.</span> and <span class="person_name">Wells, B.B.</span> and <span class="person_name">Lane, P.A.</span> and <span class="person_name">Clark, R.J.</span> (2006) <xhtml:em>RAPD marker variation in meat quality traits of Poll Dorset second-cross lambs selected for muscle or growth.</xhtml:em> In: 52nd International Congress of Meat Science and Technology, 13 - 18 August 2006, Dublin, Ireland.</p><p style="margin-bottom: 1em" class="not_ep_block"></p><table style="margin-bottom: 1em" class="not_ep_block"><tr><td valign="top" style="text-align:center"><a onmouseover="EPJS_ShowPreview( event, 'doc_preview_3997' );" onmouseout="EPJS_HidePreview( event, 'doc_preview_3997' );" href="http://eprints.utas.edu.au/2971/1/MALAU-ADULI_et_al_2006DublinPollDorsetSheep.pdf"><img alt="[img]" src="http://eprints.utas.edu.au/style/images/fileicons/application_pdf.png" border="0" class="ep_doc_icon" /></a><div class="ep_preview" id="doc_preview_3997"><table><tr><td><img alt="" src="http://eprints.utas.edu.au/2971/thumbnails/1/preview.png" border="0" class="ep_preview_image" /><div class="ep_preview_title">Preview</div></td></tr></table></div></td><td valign="top"><a href="http://eprints.utas.edu.au/2971/1/MALAU-ADULI_et_al_2006DublinPollDorsetSheep.pdf"><span class="ep_document_citation">PDF</span></a> - Requires a PDF viewer<br />197Kb</td></tr></table><p style="margin-bottom: 1em" class="not_ep_block">Official URL: <a href="http://www.cpregistrations.com/icomst2006/">http://www.cpregistrations.com/icomst2006/</a></p><div class="not_ep_block"><h2>Abstract</h2><p style="padding-bottom: 16px; text-align: left; margin: 1em auto 0em auto">All organisms are subject to mutations as a result of normal cellular operations or interactions with the environment, leading to genetic variation (polymorphism). In conjunction with selection and genetic drift, there arises genetic variation within and among individuals and species. For this variation to be useful to geneticists, it must be heritable and discernable whether as a recognisable phenotypic variation or as a genetic mutation distinguishable through molecular techniques. PCR amplification and gel electrophoresis resolution of products using RAPD primer A2 shows polymorphism of up to 5 bands was evident. It also demonstrates that at the molecular level, there are banding differences that can be picked up between crossbred progeny sired by rams with high genetic merit for growth or muscle.Fat depth at the GR and C sites was significantly (P<0.05) influenced by level of nutrition and nutrition level x siretype interaction in that fat depths were greater in crossbred lambs fed at high levels of nutrition than those fed low nutrition levels. Lambs selected for growth and fed high level of nutrition produced carcasses with the least KNIFE GR fat depth of 11.4mm, a significant reduction from 18.1mm in the control group fed low level nutrition. </p></div><table style="margin-bottom: 1em" border="0" cellpadding="3" class="not_ep_block"><tr><th valign="top" class="ep_row">Item Type:</th><td valign="top" class="ep_row">Conference or Workshop Item (Paper)</td></tr><tr><th valign="top" class="ep_row">Additional Information:</th><td valign="top" class="ep_row">In: D. Troy, R. Pearce, B. Byrne, J. Kerry (Editors), Harnessing and Exploiting Global Opportunities’, Proceedings of the 52nd International Congress of Meat Science and Technology, University College Dublin, Ireland, 52:89-90 (2006)</td></tr><tr><th valign="top" class="ep_row">Keywords:</th><td valign="top" class="ep_row">RAPD markers, Poll Dorset crossbred lambs, meat quality, growth, muscle</td></tr><tr><th valign="top" class="ep_row">Subjects:</th><td valign="top" class="ep_row"><a href="http://eprints.utas.edu.au/view/subjects/270200.html">270000 Biological Sciences > 270200 Genetics</a><br /><a href="http://eprints.utas.edu.au/view/subjects/300401.html">300000 Agricultural, Veterinary and Environmental Sciences > 300400 Animal Production > 300401 Animal Breeding</a><br /><a href="http://eprints.utas.edu.au/view/subjects/270208.html">270000 Biological Sciences > 270200 Genetics > 270208 Molecular Evolution</a><br /><a href="http://eprints.utas.edu.au/view/subjects/300400.html">300000 Agricultural, Veterinary and Environmental Sciences > 300400 Animal Production</a><br /><a href="http://eprints.utas.edu.au/view/subjects/270800.html">270000 Biological Sciences > 270800 Biotechnology</a></td></tr><tr><th valign="top" class="ep_row">Collections:</th><td valign="top" class="ep_row">UNSPECIFIED</td></tr><tr><th valign="top" class="ep_row">ID Code:</th><td valign="top" class="ep_row">2971</td></tr><tr><th valign="top" class="ep_row">Deposited By:</th><td valign="top" class="ep_row"><span class="ep_name_citation"><span class="person_name">Dr Aduli E.O. Malau-Aduli</span></span></td></tr><tr><th valign="top" class="ep_row">Deposited On:</th><td valign="top" class="ep_row">24 Jan 2008 12:10</td></tr><tr><th valign="top" class="ep_row">Last Modified:</th><td valign="top" class="ep_row">24 Jan 2008 12:10</td></tr><tr><th valign="top" class="ep_row">ePrint Statistics:</th><td valign="top" class="ep_row"><a target="ePrintStats" href="/es/index.php?action=show_detail_eprint;id=2971;">View statistics for this ePrint</a></td></tr></table><p align="right">Repository Staff Only: <a href="http://eprints.utas.edu.au/cgi/users/home?screen=EPrint::View&eprintid=2971">item control page</a></p> </td></tr></table> </div> <!-- InstanceEndEditable --></td> </tr> <tr> <td><!-- #BeginLibraryItem "/Library/footer_eprints.lbi" --> <table width="795" border="0" align="left" cellpadding="0" class="footer"> <tr valign="top"> <td colspan="2"><div align="center"><a href="http://www.utas.edu.au">UTAS home</a> | <a href="http://www.utas.edu.au/library/">Library home</a> | <a href="/">ePrints home</a> | <a href="/contact.html">contact</a> | <a href="/information.html">about</a> | <a href="/view/">browse</a> | <a href="/perl/search/simple">search</a> | <a href="/perl/register">register</a> | <a href="/perl/users/home">user area</a> | <a href="/help/">help</a></div><br /></td> </tr> <tr><td colspan="2"><p><img src="/images/eprints/footerline.gif" width="100%" height="4" /></p></td></tr> <tr valign="top"> <td width="68%" class="footer">Authorised by the University Librarian<br /> © University of Tasmania ABN 30 764 374 782<br /> <a href="http://www.utas.edu.au/cricos/">CRICOS Provider Code 00586B</a> | <a href="http://www.utas.edu.au/copyright/copyright_disclaimers.html">Copyright & Disclaimers</a> | <a href="http://www.utas.edu.au/accessibility/index.html">Accessibility</a> | <a href="http://eprints.utas.edu.au/feedback/">Site Feedback</a> </td> <td width="32%"><div align="right"> <p align="right" class="NoPrint"><a href="http://www.utas.edu.au/"><img src="http://www.utas.edu.au/shared/logos/unioftasstrip.gif" alt="University of Tasmania Home Page" width="260" height="16" border="0" align="right" /></a></p> <p align="right" class="NoPrint"><a href="http://www.utas.edu.au/"><br /> </a></p> </div></td> </tr> <tr valign="top"> <td><p> </p></td> <td><div align="right"><span class="NoPrint"><a href="http://www.eprints.org/software/"><img src="/images/eprintslogo.gif" alt="ePrints logo" width="77" height="29" border="0" align="bottom" /></a></span></div></td> </tr> </table> <!-- #EndLibraryItem --> <div align="center"></div></td> </tr> </table> </body> </html>