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<title>UTas ePrints - Isolation, amplification, and identification of ancient copepod DNA from lake sediments</title>
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<meta content="Bissett, Andrew" name="eprints.creators_name" />
<meta content="Gibson, John A.E." name="eprints.creators_name" />
<meta content="Jarman, Simon N." name="eprints.creators_name" />
<meta content="Swadling, Kerrie M." name="eprints.creators_name" />
<meta content="Cromer, Louise" name="eprints.creators_name" />
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<meta content="John.gibson@utas.edu.au" name="eprints.creators_id" />
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<meta content="Isolation, amplification, and identification of ancient copepod DNA from lake sediments" name="eprints.title" />
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<meta content="ancient DNA, copepod, Antarctica, lake, Boeckella poppei, Paralabidocera antarctica" name="eprints.keywords" />
<meta content="Species identification of copepods in lake sediments is often difficult because their remains lack diagnostic
features. It is therefore not easy to track changes in copepod biodiversity in lakes through time. We report a
method for the isolation, amplification, and identification of copepod DNA from whole lake sediments formed
in the early Holocene to the present. The method, which involves amplification of a short (~300 base pair) DNA
sequence that varies between copepod species, provides a new approach to the study of copepod paleobiodiversity.
Successful amplification of copepod DNA was possible in samples as old as 9950 calibrated 14C y BP.
Attempts failed to recover DNA from a sediment sample ca 65,000 years old. In most cases the species identified
in the sediments matched those of extant lake populations, but analysis of early-mid Holocene sediments
from one lake revealed a species that is not present today. We were able to recover copepod DNA from core samples
stored at -20 degrees C, at 4 degrees C, and preserved with polyethyleneglycol." name="eprints.abstract" />
<meta content="2005-12" name="eprints.date" />
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<meta content="Limnology and Oceanography: Methods" name="eprints.publication" />
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<meta content="Altschul, S. F., T. L. Madden, A. A. Schaffer, J. H. Zhang,
Z. Zhang, W. Miller, and D. J. Lipman. 1997. Gapped BLAST
and PSI-BLAST: a new generation of protein database search
programs. Nucleic Acids Res. 25:3389-3402.
Armstrong, T. K. C., L. G. C. Girouard, and T. J. Anchordoquy.
2002. Effects of PEGylation on the preservation of cationic
lipid/DNA complexes during freeze-thawing and
lyophilization. J. Pharm. Sci. 91:2549-2558.
Bissett et al. Ancient copepod DNA
540
Bayly, I. A. E., J. A. E. Gibson, B. Wagner, and K. M. Swadling.
2003. Taxonomy, ecology and zoogeography of two Antarctic
freshwater calanoid copepod species: Boeckella poppei
and Gladioferens antarcticus. Antarct. Sci. 15:439-448.
Cohen, A. S. 2003. Paleolimnology: the history and evolution
of lake systems. Oxford University Press.
Cole, J. R., and others. 2005. The Ribosomal Database Project
(RDP-II): sequences and tools for high-throughput rRNA
analysis. Nucleic Acids Res. 33 (Database Issue):D294-D296.
Coolen, M. J. L., and J. Overmann. 1998. Analysis of subfossil
molecular remains of purple sulfur bacteria in a lake sediment.
Appl. Environ. Microbiol. 64:4513-4521.
âÃÂÃÂâÃÂÃÂâÃÂÃÂ, E. C. Hopmans, W. I. C. Rijpstra, G. Muyzer,
S. Schouten, J. K. Volkman, and J. S. Sinninghe DamstÃÂé.
2004a. Evolution of the methane cycle in Ace Lake (Antarctica)
during the Holocene: response of methanogens and
methanotrophs to environmental changes. Org. Geochem.
35:1151-1167.
âÃÂÃÂâÃÂÃÂâÃÂÃÂ, G. Muyzer, W. I. C. Rijpstra, S. Schouten, J. K. Volkman,
and J. S. Sinninghe DamstÃÂé. 2004b. Combined DNA and
lipid analyses of sediments reveal changes in Holocene
haptophyte and diatom populations in an Antarctic lake.
Earth Planet. Sci. Lett. 223:225-239.
Cooper, A., and H. N. Poinar 2000. Ancient DNA: do it right or
not at all. Science 289:1139.
Cousyn, C., L. De Meester, J. K. Colbourne, J. K. Brendonck, D.
Verschuren, and F. Volckaert. 2001. Rapid, local adaptation
of zooplankton behavior to changes in predation pressure
in the absence of neutral genetic changes. Proc. Natl. Acad.
Sci. U S A 98:6256-6260.
Cromer, L., J. A. E. Gibson, K. M. Swadling, and D. A. Hodgson.
2005a. Evidence for a faunal refuge in the Larsemann Hills,
East Antarctica, during the Last Glacial Maximum. Quaternary
Australasia 22:12-13.
âÃÂÃÂâÃÂÃÂâÃÂÃÂ, J. A. E. Gibson, K. M. Swadling, and D. A. Ritz. 2005b.
Faunal microfossils: indicators for ecological change in an
Antarctic saline lake. Palaeogeogr. Palaeoclimatol. Palaeoecol.
221:83-97.
Dartnall, H. J. G. 1995. Rotifers, and other aquatic invertebrates,
from the Larsemann Hills, Antarctica. Pap. Proc. Roy.
Soc. Tasmania 129:17-23.
Duffy, M. A., L. J. Perry, C. M. Kearns, L. J. Weider, and N. G. Hairston.
2000. Paleogenetic evidence for a past invasion of
Onondaga Lake, NY, by exotic Daphnia curvirostris using
mtDNA from dormant eggs. Limnol. Oceanogr. 45:1409-1414.
Frey, D.G. 1964. Remains of animals in Quaternary lake and
bog sediments and their interpretation. Arch. Hydrobiol.
Beih. Ergebn. Limnol. 2:1-114.
Hairston, N. G., Jr. 1996. Zooplankton egg banks as biotic
reservoirs in changing environments. Limnol. Oceanogr.
41:1087-1092.
âÃÂÃÂâÃÂÃÂâÃÂàand A. J. Bohonak. 1998. Copepod reproductive strategies:
life-history theory, phylogenetic pattern and invasion
of inland waters. J. Mar. Systems 15:23-34.
âÃÂÃÂâÃÂÃÂâÃÂàand C. E. CÃÂáceres. 1996. Distribution of crustacean diapause:
micro- and macroevolutionary pattern and process.
Hydrobiologia 320:27-44.
Hall, T. A. 1999. BioEdit: a user-friendly biological sequence
alignment editor and analysis program for Windows
95/98/NT. Nucleic Acids Symp. Ser. 41:95-98.
Hebert, P. D. N., A. Cywinska, S. L. Ball, and J. R. deWaard.
2003. Biological identifications through DNA barcodes.
Proc. Roy. Soc. Lond. B 270:313-322.
Hodgson, D. A., P. E. Noon, and others. 2001. Were the Larsemann
Hills ice-free through the Last Glacial Maximum?
Antarct. Sci. 13:440-454.
âÃÂÃÂâÃÂÃÂâÃÂÃÂ, E. Verleyen, and others. 2005. Late Quaternary
climate-driven environmental change in the Larsemann
Hills, East Antarctica: multi-proxy evidence from a lake
sediment core. Quaternary Res. 64:83-99.
Huys, R., and G. A. Boxshall. 1991. Copepod evolution. The
Ray Society, London.
Jarman, S. N. 2004. Amplicon: software for designing PCR
primers on aligned DNA sequences. Bioinform. 20:
1644-1645.
âÃÂÃÂâÃÂÃÂâÃÂÃÂ, B. E. Deagle, and N. J. Gales. 2004. Group-specific
polymerase chain reaction for DNA-based analysis of
species identity and diversity in dietary samples. Mol. Ecol.
13:1313-1322.
Khanna, M., and G. Stotzky. 1992. Transformation of Bacillus
subtilis by DNA bound on montmorillonite and effect of
DNase on the transforming ability of bound DNA. Appl.
Environ. Microbiol. 58:1930-1939.
Leavitt, P. R., and D. A. Hodgson. 2001. Sedimentary pigments.
In J. P. Smol, H. J. B. Birks and W. M. Last [eds.],
Tracking environmental change using lake sediments. Volume
3: Terrestrial, algal and siliceous indicators. Kluwer.
Limburg, P. A., and L. J. Weider. 2002. âÃÂÃÂAncientâÃÂàDNA in the
resting egg bank of a microcrustacean can serve as a palaeolimnological
database. Proc. Roy. Soc. London B 269:
281-287.
Lorenz, M. G., and W. Wackernagel. 1987. Adsorption of DNA
to sand and variable degradation rates of adsorbed DNA.
Appl. Environ. Microbiol. 53:2948-2952.
Meyers, P. A. 1997. Organic geochemical proxies for
palaeooceanographic, paleolimnologic, and paleoclimatic
processes. Org. Geochem. 27:213-250.
âÃÂÃÂâÃÂÃÂâÃÂàand J. L. Teranes. 2001. Sediment organic matter,
p. 239-269. In W. M. Last and J. P. Smol [eds.], Tracking
environmental change using lake sediments. Volume 2:
Physical and geochemical methods. Kluwer.
McCormac, F. G., A. G. Hogg, P. G. Blackwell, C. E. Buck,
T. F. G. Higham, and P. J. Reimer. 2004. SHCal04 Southern
Hemisphere Calibration 0-11000 cal BP. Radiocarbon 46:
1087-1092.
Page, R. D. M. 1996. TREEVIEW: an application to display phylogenetic
trees on personal computers. Comput. Appl. Biosci.
12:357-358.
Bissett et al. Ancient copepod DNA
541
Rautio, M., S. Sorvari, and A. Korhola. 2000. Diatom and crustacean
zooplankton communities, their seasonal variability
and their representation in the sediment of subarctic Lake
SaanajÃÂärvi. J. Limnol. 59 (Suppl. 1):81-96.
Reid, V. A., G. R. Carvalho, and D. G. George. 2000. Molecular
genetic analysis of Daphnia in the English Lake District:
species identity, hybridization and resting egg banks.
Freshw. Biol. 44:247-253.
âÃÂÃÂâÃÂÃÂâÃÂÃÂ, G. R. Carvalho, D. G. George, and H. I. Griffiths. 2002.
A technique for the molecular genetic analysis of Daphnia
resting eggs from sub-recent lake sediments. J. Paleolimnol.
27:481-486.
Smol, J. P., H. J. B. Birks, and W. M. Last. 2001. Tracking environmental
change using lake sediments. Volume 4: Zoological
indicators. Kluwer.
âÃÂÃÂâÃÂÃÂâÃÂÃÂ, A. P. Wolfe, and others. 2005. Climate-driven regime
shifts in the biological communities of arctic lakes. Proc.
Natl. Acad. Sci. U S A 102:4397-4402.
Stuiver, M., and P. J. Reimer. 1993. Extended 14C database and
revised CALIB radiocarbon calibration program. Radiocarbon
35:215-230.
âÃÂÃÂâÃÂÃÂâÃÂÃÂ, P. J. Reimer, and R. Reimer. 2005. CALIB Radiocarbon Calibration.
Version 5.0.2. http://radiocarbon.pa.qub.ac.uk/calib/.
Swadling, K. M., A. D. McKinnon, G. DeâÃÂÃÂath, and J. A. E. Gibson.
2004. Growth, production and life cycle plasticity of the
copepod Paralabidocera antarctica in east Antarctica. Limnol.
Oceanogr. 49:644-655.
Tautz, D., P. Arctander, A. Minelli, R. H. Thomas, and A. P.
Vogler. 2003. A plea for DNA taxonomy. Trends Ecol. Evol.
18:70-74.
Thompson, J. D., T. J. Gibson, F. Plewniak, F. Jeanmougin,
and D. G. Higgins. 1997. The ClustalX windows interface:
flexible strategies for multiple sequence alignment
aided by quality analysis tools. Nucleic Acids Res. 25:
4876-4882.
Tippet, R. 1964. An investigation into the nature of the layering
of deepwater sediments in two eastern Ontario lakes.
Can. J. Bot. 4:1693-1708.
Wagner, B., H. Cremer, N. Hultzsch, D. B. Gore, and M. Melles.
2004. Late Pleistocene and Holocene history of Lake Terrasovoje,
Amery Oasis, East Antarctica, and its climatic and
environmental implications. J. Paleolimnol. 32:321-339.
Warner, B. G. 1989. Other fossils. Geosci. Can. 16:231-242.
Wayne, R. K., J. A. Leonard, and A. Cooper. 1999. Full of sound
and fury: the recent history of ancient DNA. Annu. Rev.
Ecol. Syst. 30:457-477.
Willerslev, E., A. J. Hansen, and H. N. Poinar. 2004. Isolation
of nucleic acids and cultures from fossil ice and permafrost.
Trends Ecol. Evol. 19:141-147.
Wuyts, J., P. De Rijk, Y. Van de Peer, T. Winkelmans, and R. De
Wachter. 2001. The European large subunit ribosomal RNA
database. Nucleic Acids Res. 29:175-177.
Zale, R. 1994. 14C age corrections in antarctic lake sediments
inferred from geochemistry. Radiocarbon 36:173-185.
âÃÂÃÂâÃÂÃÂâÃÂàand W. KarlÃÂén. 1989. Lake sediment cores from the
Antarctic Peninsula and surrounding islands. Geogr. Ann. A
71:211-220." name="eprints.referencetext" />
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<meta content="Species identification of copepods in lake sediments is often difficult because their remains lack diagnostic
features. It is therefore not easy to track changes in copepod biodiversity in lakes through time. We report a
method for the isolation, amplification, and identification of copepod DNA from whole lake sediments formed
in the early Holocene to the present. The method, which involves amplification of a short (~300 base pair) DNA
sequence that varies between copepod species, provides a new approach to the study of copepod paleobiodiversity.
Successful amplification of copepod DNA was possible in samples as old as 9950 calibrated 14C y BP.
Attempts failed to recover DNA from a sediment sample ca 65,000 years old. In most cases the species identified
in the sediments matched those of extant lake populations, but analysis of early-mid Holocene sediments
from one lake revealed a species that is not present today. We were able to recover copepod DNA from core samples
stored at -20 degrees C, at 4 degrees C, and preserved with polyethyleneglycol." name="DC.description" />
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<h1 class="ep_tm_pagetitle">Isolation, amplification, and identification of ancient copepod DNA from lake sediments</h1>
<p style="margin-bottom: 1em" class="not_ep_block"><span class="person_name">Bissett, Andrew</span> and <span class="person_name">Gibson, John A.E.</span> and <span class="person_name">Jarman, Simon N.</span> and <span class="person_name">Swadling, Kerrie M.</span> and <span class="person_name">Cromer, Louise</span> (2005) <xhtml:em>Isolation, amplification, and identification of ancient copepod DNA from lake sediments.</xhtml:em> Limnology and Oceanography: Methods, 3 . pp. 533-542. ISSN 1541-5856</p><p style="margin-bottom: 1em" class="not_ep_block"></p><table style="margin-bottom: 1em" class="not_ep_block"><tr><td valign="top" style="text-align:center"><a href="http://eprints.utas.edu.au/701/1/Bissett.pdf"><img alt="[img]" src="http://eprints.utas.edu.au/style/images/fileicons/application_pdf.png" class="ep_doc_icon" border="0" /></a></td><td valign="top"><a href="http://eprints.utas.edu.au/701/1/Bissett.pdf"><span class="ep_document_citation">PDF</span></a> - Full text restricted - Requires a PDF viewer<br />126Kb</td><td><form method="get" accept-charset="utf-8" action="http://eprints.utas.edu.au/cgi/request_doc"><input accept-charset="utf-8" value="714" name="docid" type="hidden" /><div class=""><input value="Request a copy" name="_action_null" class="ep_form_action_button" onclick="return EPJS_button_pushed( '_action_null' )" type="submit" /> </div></form></td></tr></table><p style="margin-bottom: 1em" class="not_ep_block">Official URL: <a href="http://aslo.org/lomethods/free/2005/index.html">http://aslo.org/lomethods/free/2005/index.html</a></p><div class="not_ep_block"><h2>Abstract</h2><p style="padding-bottom: 16px; text-align: left; margin: 1em auto 0em auto">Species identification of copepods in lake sediments is often difficult because their remains lack diagnostic
features. It is therefore not easy to track changes in copepod biodiversity in lakes through time. We report a
method for the isolation, amplification, and identification of copepod DNA from whole lake sediments formed
in the early Holocene to the present. The method, which involves amplification of a short (~300 base pair) DNA
sequence that varies between copepod species, provides a new approach to the study of copepod paleobiodiversity.
Successful amplification of copepod DNA was possible in samples as old as 9950 calibrated 14C y BP.
Attempts failed to recover DNA from a sediment sample ca 65,000 years old. In most cases the species identified
in the sediments matched those of extant lake populations, but analysis of early-mid Holocene sediments
from one lake revealed a species that is not present today. We were able to recover copepod DNA from core samples
stored at -20 degrees C, at 4 degrees C, and preserved with polyethyleneglycol.</p></div><table style="margin-bottom: 1em" cellpadding="3" class="not_ep_block" border="0"><tr><th valign="top" class="ep_row">Item Type:</th><td valign="top" class="ep_row">Article</td></tr><tr><th valign="top" class="ep_row">Keywords:</th><td valign="top" class="ep_row">ancient DNA, copepod, Antarctica, lake, Boeckella poppei, Paralabidocera antarctica</td></tr><tr><th valign="top" class="ep_row">Subjects:</th><td valign="top" class="ep_row"><a href="http://eprints.utas.edu.au/view/subjects/270299.html">270000 Biological Sciences > 270200 Genetics > 270299 Genetics not elsewhere classified</a></td></tr><tr><th valign="top" class="ep_row">Collections:</th><td valign="top" class="ep_row">UNSPECIFIED</td></tr><tr><th valign="top" class="ep_row">ID Code:</th><td valign="top" class="ep_row">701</td></tr><tr><th valign="top" class="ep_row">Deposited By:</th><td valign="top" class="ep_row"><span class="ep_name_citation"><span class="person_name">Dr John A.E. Gibson</span></span></td></tr><tr><th valign="top" class="ep_row">Deposited On:</th><td valign="top" class="ep_row">08 Feb 2007</td></tr><tr><th valign="top" class="ep_row">Last Modified:</th><td valign="top" class="ep_row">08 Feb 2008 15:26</td></tr><tr><th valign="top" class="ep_row">ePrint Statistics:</th><td valign="top" class="ep_row"><a target="ePrintStats" href="/es/index.php?action=show_detail_eprint;id=701;">View statistics for this ePrint</a></td></tr></table><p align="right">Repository Staff Only: <a href="http://eprints.utas.edu.au/cgi/users/home?screen=EPrint::View&eprintid=701">item control page</a></p>
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