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<title>UTas ePrints - Effect of extended-spectrum beta-lactamases on the susceptibility of Haemophilus influenzae to cephalosporins</title>
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<meta content="Tristram, Stephen G" name="eprints.creators_name" />
<meta content="Stephen.Tristram@utas.edu.au" name="eprints.creators_id" />
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<meta content="2007-03-16" name="eprints.datestamp" />
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<meta content="extended-spectrum beta-lactamase, TEM beta-lactamase" name="eprints.keywords" />
<meta content="This is a pre-copy-editing, author-produced PDF of an article accepted for publication in Journal of Antimicrobial Chemotherapy following peer review. The definitive publisher-authenticated version Tristram, Stephen G (2003) Effect of extended-spectrum beta-lactamases on the susceptibility of Haemophilus influenzae to cephalosporins. Journal of Antimicrobial Chemotherapy, 51. pp. 39-43 is available online at:http://jac.oxfordjournals.org/
" name="eprints.note" />
<meta content="The extended-spectrum beta-lactamases (ESBLs) TEM-3, TEM-4 and TEM-5 were cloned into
Haemophilus influenzae. These recombinant strains exhibited cefotaxime MICs of 0.5, 0.25 and
0.12 mg/L for TEM-3, -4 and -5, respectively, and the MIC of cefaclor was 4.0 mg/L. These MICs are
higher than those of beta-lactamase-negative strains, or those producing simple wild-type TEM-1
beta-lactamase, but not high enough to be categorized as resistant according to the breakpoints of
the NCCLS. The clones were also categorized as susceptible using NCCLS disc diffusion
methodology and interpretive criteria. This study shows that current NCCLS susceptibility
testing methods may have difficulty in detecting ESBLs if they were to occur in H. influenzae.
" name="eprints.abstract" />
<meta content="2003" name="eprints.date" />
<meta content="published" name="eprints.date_type" />
<meta content="Journal of Antimicrobial Chemotherapy" name="eprints.publication" />
<meta content="51" name="eprints.volume" />
<meta content="39-43" name="eprints.pagerange" />
<meta content="10.1093/jac/dkg015" name="eprints.id_number" />
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1. Sutcliffe, J. G. (1978). Nucleotide sequence of the ampicillin
resistance gene of Escherichia coli plasmid pBR322. Proceedings
of the National Academy of Sciences, USA 75, 3737–41.
2. Goussard, S. & Courvalin, P. (1991). Sequences of the genes
blaT-1B and blaT-2. Gene 102, 71–3.
3. Levesque, R. C. & Jacoby, G. A. (1988). Molecular structure
and interrelationships of multiresistance β-lactamase transposons.
Plasmid 19, 21–9.
4. Laufs, R., Riess, F., Jahn, G., Fock, R. & Kaulfers, P. (1981).
Origin of Haemophilus influenzae R factors. Journal of Bacteriology
147, 563–8.
5. Doern, G. V. & The Alexander Project Collaborative Group
(1996). Antimicrobial resistance among lower respiratory tract
isolates of Haemophilus influenzae: results of a 1992–93 Western
Europe and USA collaborative surveillance study. Journal of Antimicrobial
Chemotherapy 38, Suppl. A, 59–69.
6. Jorgensen, J. H. (1992). Update on mechanisms and prevalence
of antimicrobial resistance in Haemophilus influenzae. Clinical
Infectious Diseases 14, 1119–23.
7. Powell, M. (1991). Chemotherapy for infections caused by
Haemophilus influenzae: current problems and future prospects.
Journal of Antimicrobial Chemotherapy 27, 3–7.
8. Mendelman, P. M., Chaffin, D. O. & Kalaitzoglou, G. (1990).
Penicillin-binding proteins and ampicillin resistance in Haemophilus
influenzae. Journal of Antimicrobial Chemotherapy 25, 525–34.
9. James, P. A., Lewis, D. A., Jordens, J. Z., Cribb, J., Dawson,
S. J. & Murray, S. A. (1996). The incidence and epidemiology of
beta-lactam resistance in Haemophilus influenzae. Journal of Antimicrobial
Chemotherapy 37, 737–46.
10. Jacoby, G. A. (1994). Genetics of extended spectrum
β-lactamases. European Journal of Clinical Microbiology and
Infectious Diseases 13, Suppl. 1, 2–11.
11. Jacoby, G. A. & Medeiros, A. (1991). More extended spectrum
β-lactamases. Antimicrobial Agents and Chemotherapy 35, 1697–
704.
12. Dimopoulou, I. D., Kraak, W. A., Anderson, E. C., Nichols,
W. W., Slack, M. P. & Crook, D. W. (1992). Molecular epidemiology
of unrelated clusters of multiresistant strains of Haemophilus
influenzae. Journal of Infectious Diseases 165, 1069–75.
13. Tristram, S. G. (1999). Extended spectrum β-lactamases in
Haemophilus? Australian Journal of Medical Science 20, 33–41.
14. Philippon, A., Redjeb, S. B., Fournier, G. & Hassen, A. B.
(1989). New plasmid borne β-lactamases. Infection 17, 347–54.
15. Doern, G. V. & Jones, R. N. (1993). In vitro susceptibility test
practices with Haemophilus influenzae among College of American
Pathologists survey participants in the United States. Diagnostic
Microbiology and Infectious Disease 17, 61–5.
16. Jones, R. N., Thornsberry, C., Hindler, J., Gerlach, E. H. &
Doern, G. V. (1992). The re-evaluation of the NCCLS ampicillin
susceptibility tests with Haemophilus test medium: new interpretive
criteria for the disk diffusion method. Antimicrobial Newsletter 8,
31–5.
17. Sougakoff, W., Goussard, S., Gerbaud, G. & Courvalin, P.
(1988). Plasmid mediated resistance to third generation cephalosporins
caused by point mutations in TEM-type penicillinase genes.
Reviews of Infectious Diseases 10, 879–84.
18. Sougakoff, W., Petit, A., Goussard, S., Sirot, D., Bure, A. &
Courvalin, P. (1989). Characterization of the plasmid genes blaT-4
and blaT-5 which encode the broad spectrum β-lactamases
TEM-4 and TEM-5 in Enterobacteriaceae. Gene 78, 339–48.
19. Willson, P. J., Albritton, W. L., Slaney, L. & Setlow, J. K. (1989).
Characterization of a multiple antibiotic resistance plasmid from
Haemophilus ducreyi. Antimicrobial Agents and Chemotherapy 33,
1627–30.
20. Balko, T., Karlowsky, J. A., Palatnick, L. P., Zhanel, G. G. &
Hoban, D. J. (1999). Characterization of the inoculum effect with
Haemophilus influenzae and β-lactams. Diagnostic Microbiology
and Infectious Disease 33, 47–58.
21. Barcak, G. J., Chandler, M. S., Redfield, R. J. & Tomb, J.
(1991). Genetic systems in Haemophilus influenzae. Methods in
Enzymology 204, 321–42.
22. Brun-Buisson, C., Legrand, P., Philipon, A., Montravers, F.,
Ansquer, M. & Duval, J. (1987). Tranferable enzymatic resistance to
third generation cephalosporins during a nosocomial outbreak of
multiresistant Klebsiella pneumoniae. Lancet ii, 302–6.
23. Chanal, C., Poupart, M., Sirot, D., Labia, R., Sirot, J. & Cluzel,
R. (1992). Nucleotide sequences of CAZ-2, CAZ-6, and CAZ-7
β-lactamase genes. Antimicrobial Agents and Chemotherapy 36,
1817–20.
24. National Committee for Clinical Laboratory Standards. (2000).
Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria
that Grow Aerobically—Fifth Edition: Approved Standard M7-A5.
NCCLS, Villanova, PA, USA.
25. National Committee for Clinical Laboratory Standards. (2000).
Performance Standards for Antimicrobial Disk Susceptibility
Tests—Sixth Edition: Approved Standard M2-A7. NCCLS, Villanova,
PA, USA.
26. James, P. A. & Reeves, D. S. (1996). Bacterial resistance to
cephalosporins as a function of outer membrane permeability and
access to their target. Journal of Chemotherapy 8, Suppl. 2, 37–47.
27. Wu, P., Shannon, K. & Phillips, I. (1995). Mechanisms of hyperproduction
of TEM-1 β-lactamase by clinical isolates of Escherichia
coli. Journal of Antimicrobial Chemotherapy 36, 927–39.
28. Jacoby, G. & Bush, K. (2002). Amino acid sequences for TEM,
SHV and OXA extended-spectrum and inhibitor resistant β-lactamases.
[Online.] http://www.lahey.org/studies/webt.stm (10 July 2002,
date last accessed).
29. Bonnet, R., De Champs, C., Sirot, D., Chanal, C., Labia, R. &
Sirot, J. (1999). Diversity of TEM mutants in Proteus mirabilis. Antimicrobial
Agents and Chemotherapy 43, 2671–7.
30. Medeiros, A. A. & O’Brien, T. F. (1975). Ampicillin-resistant
Haemophiluis influenzae type B possessing a TEM-type β-lactamase
but little permeability barrier to ampicillin. Lancet i, 716–8.
31. Verbist, L. (1993). Relevance of antibiotic susceptibility testing
for clinical practice. European Journal of Clinical Microbiology and
Infectious Diseases 12, Suppl. 1, 2–5.
32. James, P. A., Hossain, F. K., Lewis, D. A. & White, D. G. (1993).
β-Lactam susceptibility of Haemophilus influenzae strains showing
reduced susceptibility to cefuroxime. Journal of Antimicrobial
Chemotherapy 32, 239–46.
33. Dagan, R., Abramson, O., Leibovitz, E., Greenberg, D., Lang,
R., Goshen, S. et al. (1997). Bacteriologic response to oral cephalosporins:
are established susceptibility breakpoints appropriate in the
case of acute otitis media? Journal of Infectious Diseases 178,
1253–9." name="eprints.referencetext" />
<meta content="Tristram, Stephen G (2003) Effect of extended-spectrum beta-lactamases on the susceptibility of Haemophilus influenzae to cephalosporins. Journal of Antimicrobial Chemotherapy, 51 . pp. 39-43. ISSN 0305-7453" name="eprints.citation" />
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<meta content="The extended-spectrum beta-lactamases (ESBLs) TEM-3, TEM-4 and TEM-5 were cloned into
Haemophilus influenzae. These recombinant strains exhibited cefotaxime MICs of 0.5, 0.25 and
0.12 mg/L for TEM-3, -4 and -5, respectively, and the MIC of cefaclor was 4.0 mg/L. These MICs are
higher than those of beta-lactamase-negative strains, or those producing simple wild-type TEM-1
beta-lactamase, but not high enough to be categorized as resistant according to the breakpoints of
the NCCLS. The clones were also categorized as susceptible using NCCLS disc diffusion
methodology and interpretive criteria. This study shows that current NCCLS susceptibility
testing methods may have difficulty in detecting ESBLs if they were to occur in H. influenzae.
" name="DC.description" />
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<h1 class="ep_tm_pagetitle">Effect of extended-spectrum beta-lactamases on the susceptibility of Haemophilus influenzae to cephalosporins</h1>
<p style="margin-bottom: 1em" class="not_ep_block"><span class="person_name">Tristram, Stephen G</span> (2003) <xhtml:em>Effect of extended-spectrum beta-lactamases on the susceptibility of Haemophilus influenzae to cephalosporins.</xhtml:em> Journal of Antimicrobial Chemotherapy, 51 . pp. 39-43. ISSN 0305-7453</p><p style="margin-bottom: 1em" class="not_ep_block"></p><table style="margin-bottom: 1em" class="not_ep_block"><tr><td valign="top" style="text-align:center"><a href="http://eprints.utas.edu.au/852/1/ESBLs_in_Hi.pdf"><img alt="[img]" src="http://eprints.utas.edu.au/style/images/fileicons/application_pdf.png" border="0" class="ep_doc_icon" /></a></td><td valign="top"><a href="http://eprints.utas.edu.au/852/1/ESBLs_in_Hi.pdf"><span class="ep_document_citation">PDF</span></a> - Full text restricted - Requires a PDF viewer<br />99Kb</td><td><form method="get" accept-charset="utf-8" action="http://eprints.utas.edu.au/cgi/request_doc"><input value="859" name="docid" accept-charset="utf-8" type="hidden" /><div class=""><input value="Request a copy" name="_action_null" class="ep_form_action_button" onclick="return EPJS_button_pushed( '_action_null' )" type="submit" /> </div></form></td></tr></table><p style="margin-bottom: 1em" class="not_ep_block">Official URL: <a href="http://dx.doi.org/10.1093/jac/dkg015">http://dx.doi.org/10.1093/jac/dkg015</a></p><div class="not_ep_block"><h2>Abstract</h2><p style="padding-bottom: 16px; text-align: left; margin: 1em auto 0em auto">The extended-spectrum beta-lactamases (ESBLs) TEM-3, TEM-4 and TEM-5 were cloned into
Haemophilus influenzae. These recombinant strains exhibited cefotaxime MICs of 0.5, 0.25 and
0.12 mg/L for TEM-3, -4 and -5, respectively, and the MIC of cefaclor was 4.0 mg/L. These MICs are
higher than those of beta-lactamase-negative strains, or those producing simple wild-type TEM-1
beta-lactamase, but not high enough to be categorized as resistant according to the breakpoints of
the NCCLS. The clones were also categorized as susceptible using NCCLS disc diffusion
methodology and interpretive criteria. This study shows that current NCCLS susceptibility
testing methods may have difficulty in detecting ESBLs if they were to occur in H. influenzae.
</p></div><table style="margin-bottom: 1em" border="0" cellpadding="3" class="not_ep_block"><tr><th valign="top" class="ep_row">Item Type:</th><td valign="top" class="ep_row">Article</td></tr><tr><th valign="top" class="ep_row">Additional Information:</th><td valign="top" class="ep_row">This is a pre-copy-editing, author-produced PDF of an article accepted for publication in Journal of Antimicrobial Chemotherapy following peer review. The definitive publisher-authenticated version Tristram, Stephen G (2003) Effect of extended-spectrum beta-lactamases on the susceptibility of Haemophilus influenzae to cephalosporins. Journal of Antimicrobial Chemotherapy, 51. pp. 39-43 is available online at:http://jac.oxfordjournals.org/
</td></tr><tr><th valign="top" class="ep_row">Keywords:</th><td valign="top" class="ep_row">extended-spectrum beta-lactamase, TEM beta-lactamase</td></tr><tr><th valign="top" class="ep_row">Subjects:</th><td valign="top" class="ep_row"><a href="http://eprints.utas.edu.au/view/subjects/320400.html">320000 Medical and Health Sciences > 320400 Medical Microbiology</a><br /><a href="http://eprints.utas.edu.au/view/subjects/270306.html">270000 Biological Sciences > 270300 Microbiology > 270306 Microbial Genetics</a><br /><a href="http://eprints.utas.edu.au/view/subjects/270304.html">270000 Biological Sciences > 270300 Microbiology > 270304 Infectious Agents</a></td></tr><tr><th valign="top" class="ep_row">Collections:</th><td valign="top" class="ep_row">UNSPECIFIED</td></tr><tr><th valign="top" class="ep_row">ID Code:</th><td valign="top" class="ep_row">852</td></tr><tr><th valign="top" class="ep_row">Deposited By:</th><td valign="top" class="ep_row"><span class="ep_name_citation"><span class="person_name">Mr Stephen G Tristram</span></span></td></tr><tr><th valign="top" class="ep_row">Deposited On:</th><td valign="top" class="ep_row">16 Mar 2007</td></tr><tr><th valign="top" class="ep_row">Last Modified:</th><td valign="top" class="ep_row">08 Feb 2008 15:54</td></tr><tr><th valign="top" class="ep_row">ePrint Statistics:</th><td valign="top" class="ep_row"><a target="ePrintStats" href="/es/index.php?action=show_detail_eprint;id=852;">View statistics for this ePrint</a></td></tr></table><p align="right">Repository Staff Only: <a href="http://eprints.utas.edu.au/cgi/users/home?screen=EPrint::View&eprintid=852">item control page</a></p>
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