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    <title>UTas ePrints - Effect of extended-spectrum beta-lactamases on the susceptibility of Haemophilus influenzae to cephalosporins</title>
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    <meta content="Tristram, Stephen G" name="eprints.creators_name" />
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<meta content="The extended-spectrum beta-lactamases (ESBLs) TEM-3, TEM-4 and TEM-5 were cloned into
Haemophilus influenzae. These recombinant strains exhibited cefotaxime MICs of 0.5, 0.25 and
0.12 mg/L for TEM-3, -4 and -5, respectively, and the MIC of cefaclor was 4.0 mg/L. These MICs are
higher than those of beta-lactamase-negative strains, or those producing simple wild-type TEM-1
beta-lactamase, but not high enough to be categorized as resistant according to the breakpoints of
the NCCLS. The clones were also categorized as susceptible using NCCLS disc diffusion
methodology and interpretive criteria. This study shows that current NCCLS susceptibility
testing methods may have difficulty in detecting ESBLs if they were to occur in H. influenzae.
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Plasmid 19, 21–9.
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Origin of Haemophilus influenzae R factors. Journal of Bacteriology
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of unrelated clusters of multiresistant strains of Haemophilus
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Doern, G. V. (1992). The re-evaluation of the NCCLS ampicillin
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17. Sougakoff, W., Goussard, S., Gerbaud, G. &amp; Courvalin, P.
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18. Sougakoff, W., Petit, A., Goussard, S., Sirot, D., Bure, A. &amp;
Courvalin, P. (1989). Characterization of the plasmid genes blaT-4
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1627–30.
20. Balko, T., Karlowsky, J. A., Palatnick, L. P., Zhanel, G. G. &amp;
Hoban, D. J. (1999). Characterization of the inoculum effect with
Haemophilus influenzae and β-lactams. Diagnostic Microbiology
and Infectious Disease 33, 47–58.
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(1991). Genetic systems in Haemophilus influenzae. Methods in
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22. Brun-Buisson, C., Legrand, P., Philipon, A., Montravers, F.,
Ansquer, M. &amp; Duval, J. (1987). Tranferable enzymatic resistance to
third generation cephalosporins during a nosocomial outbreak of
multiresistant Klebsiella pneumoniae. Lancet ii, 302–6.
23. Chanal, C., Poupart, M., Sirot, D., Labia, R., Sirot, J. &amp; Cluzel,
R. (1992). Nucleotide sequences of CAZ-2, CAZ-6, and CAZ-7
β-lactamase genes. Antimicrobial Agents and Chemotherapy 36,
1817–20.
24. National Committee for Clinical Laboratory Standards. (2000).
Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria
that Grow Aerobically—Fifth Edition: Approved Standard M7-A5.
NCCLS, Villanova, PA, USA.
25. National Committee for Clinical Laboratory Standards. (2000).
Performance Standards for Antimicrobial Disk Susceptibility
Tests—Sixth Edition: Approved Standard M2-A7. NCCLS, Villanova,
PA, USA.
26. James, P. A. &amp; Reeves, D. S. (1996). Bacterial resistance to
cephalosporins as a function of outer membrane permeability and
access to their target. Journal of Chemotherapy 8, Suppl. 2, 37–47.
27. Wu, P., Shannon, K. &amp; Phillips, I. (1995). Mechanisms of hyperproduction
of TEM-1 β-lactamase by clinical isolates of Escherichia
coli. Journal of Antimicrobial Chemotherapy 36, 927–39.
28. Jacoby, G. &amp; Bush, K. (2002). Amino acid sequences for TEM,
SHV and OXA extended-spectrum and inhibitor resistant β-lactamases.
[Online.] http://www.lahey.org/studies/webt.stm (10 July 2002,
date last accessed).
29. Bonnet, R., De Champs, C., Sirot, D., Chanal, C., Labia, R. &amp;
Sirot, J. (1999). Diversity of TEM mutants in Proteus mirabilis. Antimicrobial
Agents and Chemotherapy 43, 2671–7.
30. Medeiros, A. A. &amp; O’Brien, T. F. (1975). Ampicillin-resistant
Haemophiluis influenzae type B possessing a TEM-type β-lactamase
but little permeability barrier to ampicillin. Lancet i, 716–8.
31. Verbist, L. (1993). Relevance of antibiotic susceptibility testing
for clinical practice. European Journal of Clinical Microbiology and
Infectious Diseases 12, Suppl. 1, 2–5.
32. James, P. A., Hossain, F. K., Lewis, D. A. &amp; White, D. G. (1993).
β-Lactam susceptibility of Haemophilus influenzae strains showing
reduced susceptibility to cefuroxime. Journal of Antimicrobial
Chemotherapy 32, 239–46.
33. Dagan, R., Abramson, O., Leibovitz, E., Greenberg, D., Lang,
R., Goshen, S. et al. (1997). Bacteriologic response to oral cephalosporins:
are established susceptibility breakpoints appropriate in the
case of acute otitis media? Journal of Infectious Diseases 178,
1253–9." name="eprints.referencetext" />
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    <h1 class="ep_tm_pagetitle">Effect of extended-spectrum beta-lactamases on the susceptibility of Haemophilus influenzae to cephalosporins</h1>
    <p style="margin-bottom: 1em" class="not_ep_block"><span class="person_name">Tristram, Stephen G</span> (2003) <xhtml:em>Effect of extended-spectrum beta-lactamases on the susceptibility of Haemophilus influenzae to cephalosporins.</xhtml:em> Journal of Antimicrobial Chemotherapy, 51 . pp. 39-43. ISSN 0305-7453</p><p style="margin-bottom: 1em" class="not_ep_block"></p><table style="margin-bottom: 1em" class="not_ep_block"><tr><td valign="top" style="text-align:center"><a href="http://eprints.utas.edu.au/852/1/ESBLs_in_Hi.pdf"><img alt="[img]" src="http://eprints.utas.edu.au/style/images/fileicons/application_pdf.png" border="0" class="ep_doc_icon" /></a></td><td valign="top"><a href="http://eprints.utas.edu.au/852/1/ESBLs_in_Hi.pdf"><span class="ep_document_citation">PDF</span></a> - Full text restricted - Requires a PDF viewer<br />99Kb</td><td><form method="get" accept-charset="utf-8" action="http://eprints.utas.edu.au/cgi/request_doc"><input value="859" name="docid" accept-charset="utf-8" type="hidden" /><div class=""><input value="Request a copy" name="_action_null" class="ep_form_action_button" onclick="return EPJS_button_pushed( '_action_null' )" type="submit" /> </div></form></td></tr></table><p style="margin-bottom: 1em" class="not_ep_block">Official URL: <a href="http://dx.doi.org/10.1093/jac/dkg015">http://dx.doi.org/10.1093/jac/dkg015</a></p><div class="not_ep_block"><h2>Abstract</h2><p style="padding-bottom: 16px; text-align: left; margin: 1em auto 0em auto">The extended-spectrum beta-lactamases (ESBLs) TEM-3, TEM-4 and TEM-5 were cloned into&#13;
Haemophilus influenzae. These recombinant strains exhibited cefotaxime MICs of 0.5, 0.25 and&#13;
0.12 mg/L for TEM-3, -4 and -5, respectively, and the MIC of cefaclor was 4.0 mg/L. These MICs are&#13;
higher than those of beta-lactamase-negative strains, or those producing simple wild-type TEM-1&#13;
beta-lactamase, but not high enough to be categorized as resistant according to the breakpoints of&#13;
the NCCLS. The clones were also categorized as susceptible using NCCLS disc diffusion&#13;
methodology and interpretive criteria. This study shows that current NCCLS susceptibility&#13;
testing methods may have difficulty in detecting ESBLs if they were to occur in H. influenzae.&#13;
</p></div><table style="margin-bottom: 1em" border="0" cellpadding="3" class="not_ep_block"><tr><th valign="top" class="ep_row">Item Type:</th><td valign="top" class="ep_row">Article</td></tr><tr><th valign="top" class="ep_row">Additional Information:</th><td valign="top" class="ep_row">This is a pre-copy-editing, author-produced PDF of an article accepted for publication in Journal of Antimicrobial Chemotherapy following peer review. The definitive publisher-authenticated version Tristram, Stephen G (2003) Effect of extended-spectrum beta-lactamases on the susceptibility of Haemophilus influenzae to cephalosporins. Journal of Antimicrobial Chemotherapy, 51. pp. 39-43 is available online at:http://jac.oxfordjournals.org/ &#13;
</td></tr><tr><th valign="top" class="ep_row">Keywords:</th><td valign="top" class="ep_row">extended-spectrum beta-lactamase, TEM beta-lactamase</td></tr><tr><th valign="top" class="ep_row">Subjects:</th><td valign="top" class="ep_row"><a href="http://eprints.utas.edu.au/view/subjects/320400.html">320000 Medical and Health Sciences &gt; 320400 Medical Microbiology</a><br /><a href="http://eprints.utas.edu.au/view/subjects/270306.html">270000 Biological Sciences &gt; 270300 Microbiology &gt; 270306 Microbial Genetics</a><br /><a href="http://eprints.utas.edu.au/view/subjects/270304.html">270000 Biological Sciences &gt; 270300 Microbiology &gt; 270304 Infectious Agents</a></td></tr><tr><th valign="top" class="ep_row">Collections:</th><td valign="top" class="ep_row">UNSPECIFIED</td></tr><tr><th valign="top" class="ep_row">ID Code:</th><td valign="top" class="ep_row">852</td></tr><tr><th valign="top" class="ep_row">Deposited By:</th><td valign="top" class="ep_row"><span class="ep_name_citation"><span class="person_name">Mr Stephen G Tristram</span></span></td></tr><tr><th valign="top" class="ep_row">Deposited On:</th><td valign="top" class="ep_row">16 Mar 2007</td></tr><tr><th valign="top" class="ep_row">Last Modified:</th><td valign="top" class="ep_row">08 Feb 2008 15:54</td></tr><tr><th valign="top" class="ep_row">ePrint Statistics:</th><td valign="top" class="ep_row"><a target="ePrintStats" href="/es/index.php?action=show_detail_eprint;id=852;">View statistics for this ePrint</a></td></tr></table><p align="right">Repository Staff Only: <a href="http://eprints.utas.edu.au/cgi/users/home?screen=EPrint::View&amp;eprintid=852">item control page</a></p>
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