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  5.     <title>UTas ePrints - Ecological and biogeographic relationships of class Flavobacteria in the Southern Ocean</title>
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  28. <meta content="The abundance, spatial distribution and diversity of class Flavobacteria were investigated in the Southern Ocean euphotic zone across a latitudinal transect and in the ice pack off Eastern Antarctica. Surface seawater samples filter-fractionated into 0.8 μm particulate and 0.2 m planktonic fractions were investigated with different molecular techniques. The abundance of particle-associated Flavobacteria, ascertained with real-time PCR and DGGE band analysis using Flavobacteria-specific primers, was found to be significantly higher in Polar Front Zone (PFZ) and Antarctic Zone (AZ) water samples than in nutrient limited Temperate Zone (TZ) and Sub-Antarctic Zone (SAZ) waters. Abundance of particle-associated Flavobacteria correlated positively with seawater chlorophyll a and nutrient concentrations, suggesting that increased Flavobacteria abundance may relate to enhanced primary production in the PFZ and AZ. This is supported by comparison of DGGE profiles that demonstrated significant differences in the total Flavobacteria community structure and 16S rRNA gene diversity between samples from the PFZ and AZ and those from TZ and SAZ. Sequence analysis revealed a broad diversity amongst class Flavobacteria in the Southern Ocean with several Flavobacteria clades detected in PFZ and AZ waters not detected in TZ and SAZ waters that putatively represent psychrophilic taxa. Sequence data included a large, so far uncultivated, cosmopolitan phylogenetic clade (&quot;DE cluster 2&quot;) that is distributed throughout the Southern Ocean.
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  38. <meta content="[1]Fenchel, T. (1988) Marine plankton food-chains. Annu. Rev. Ecol. Syst. 18, 19–38.
  39.  
  40. [2]Cole, J.J, Findlay, S., Pace, M.L. (1988) Bacterial production in fresh and saltwater ecosystems – a cross-system overview. Mar. Ecol. Prog. Ser. 43, 1–10.
  41. ISI
  42. [3]Azam, F. (1998) Microbial control of oceanic carbon flux: The plot thickens. Science 280, 694–696.
  43. CrossRef, ISI
  44. [4]Bidle, K.D., Brzezinski, M.A., Long, R.A., Jones, J.L., Azam, F. (2003) Diminished efficiency in the oceanic silica pump caused by bacteria-mediated silica dissolution. Limnol. Oceanogr. 48, 1855–1868.
  45. ISI
  46. [5]Brown, G.E., Parks, G.A. (2001) Sorption of trace elements on mineral surfaces: Modern perspectives from spectroscopic studies, and comments on sorption in the marine environment. Int. Geol. Rev. 43, 963–1073.
  47. ISI
  48. [6]Kirchman, D.L. (2002) The ecology of Cytophaga–Flavobacteria in aquatic environments. FEMS Microbiol. Ecol. 39, 91–100.
  49. Synergy, ISI, Chemport
  50. [7]Kirchman, D.L., Yu, L.Y., Cottrell, M.T. (2003) Diversity and abundance of uncultured Cytophaga-like bacteria in the Delaware Estuary. Appl. Environ. Microbiol. 69, 6587–6596.
  51. CrossRef, Medline, ISI, Chemport
  52. [8]O'sullivan, L.A., Fuller, K.E., Thomas, E.M., Turley, C.M., Fry, J.C., Weightman, A.J. (2004) Distribution and culturability of the uncultivated 'AGG58 cluster' of the Bacteroidetes phylum in aquatic environments. FEMS Microbiol. Ecol. 47, 359–370.
  53. Synergy, ISI
  54. [9]DeLong, E.F., Franks, D.G., Alldredge, A.L. (1993) Phylogenetic diversity of aggregate-attached vs. free-living marine bacterial assemblages. Limnol. Oceanogr. 38, 924–934.
  55. ISI
  56. [10]Bowman, J.P., Mancuso Nichols, C.A., Gibson, J.A.E. (2003) Algoriphagus ratkowskyi gen. nov., sp. nov., Brumimicrobium glaciale gen. nov., sp. nov., Cryomorpha ignava gen. nov., sp. nov., Crocinitomix catalasitica gen. nov., sp. nov., novel flavobacteria isolated from various polar habitats. Int. J. Syst. Evol. Microbiol. 53, 1343–1355.
  57. Medline, ISI
  58. [11]Weller, R., Glöckner, F.O., Amann, R. (2000) 16S rRNA-targeted oligonucleotide probes for the in situ detection of members of the phylum Cytophaga–Flavobacterium–Bacteroides. Syst. Appl. Microbiol. 23, 107–114.
  59. Medline, ISI, Chemport
  60. [12]Ploug, H., Grossart, H.P., Azam, F., Jorgensen, B.B. (1999) Photosynthesis, respiration, and carbon turnover in sinking marine snow from surface waters of Southern California Bight: implications for the carbon cycle in the ocean. Mar. Ecol. Prog. Ser. 179, 1–11.
  61. ISI
  62. [13]Brown, M.V., Bowman, J.P. (2001) A molecular phylogenetic survey of sea-ice microbial communities (SIMCO). FEMS Microbiol. Ecol. 35, 267–275.
  63. Synergy, Medline, ISI, Chemport
  64. [14]Brinkmeyer, R., Knittel, K., Jurgens, J., Weyland, H., Amann, R., Helmke, E. (2003) Diversity and structure of bacterial communities in Arctic versus Antarctic pack ice. Appl. Environ. Microbiol. 69, 6610–6619.
  65. CrossRef, Medline, ISI, Chemport
  66. [15]Llobet-Brossa, E., Rossello-Mora, R., Amann, R. (1998) Microbial community composition of Wadden Sea sediments as revealed by fluorescence in situ hybridization. Appl. Environ. Microbiol. 64, 2691–2696.
  67. Medline, ISI
  68. [16]Bowman, J.P., McCuaig, R.D. (2003) Diversity and biogeography of prokaryotes dwelling in Antarctic continental shelf sediment. Appl. Environ. Microbiol. 69, 2463–2484.
  69. CrossRef, Medline, ISI
  70. [17]Webster, N.S., Negri, A.P., Munro, M.M.H.G., Battershill, C.N. (2004) Diverse microbial communities inhabit Antarctic sponges. Environ. Microbiol. 6, 288–300.
  71. Synergy, Medline, ISI
  72. [18]Cottrell, M.T., Kirchman, D.L. (2000) Natural assemblages of marine proteobacteria and members of the Cytophaga–Flavobacter cluster consuming low- and high-molecular-weight dissolved organic matter. Appl. Environ. Microbiol. 66, 1692–1697.
  73. CrossRef, Medline, ISI, Chemport
  74. [19]Mittelbach, G.G., Steiner, C.F., Steiner, S.M., Gross, K.L., Reynolds, H.L., Waide, R.B., Willig, M.R., Dodson, S.I., Gough, L. (2001) What is the observed relationship between species richness and productivity?. Ecology 82, 2381–2396.
  75. CrossRef, ISI
  76. [20]Horner-Devine, C.M., Leibold, M.A., Smith, V.H., Bohannan, B.J.M. (2003) Bacterial diversity patterns along a gradient of primary productivity. Ecol. Lett. 6, 613–622.
  77. Synergy, ISI
  78. [21]Kopczynska, E.E., Dehairs, F., Elskens, M., Wright, S. (2001) Phytoplankton and microzooplankton variability between the Subtropical and Polar Fronts south of Australia: Thriving under regenerative and new production in late summer. J. Geophys. Res.-Oceans 106, 31597–31609.
  79. CrossRef, ISI
  80. [22]Glöckner, F.O., Fuchs, B.M., Amann, R. (1999) Bacterioplankton compositions of lakes and oceans: a first comparison based on fluorescence in situ hybridization. Appl. Environ. Microbiol. 65, 3721–3726.
  81. Medline, ISI, Chemport
  82. [23]Lebaron, P., Servais, P., Troussellier, M., Courties, C., Vives-Rego, J., Muyzer, G., Bernard, L., Guindulain, T., Schafer, H., Stackebrandt, E. (1999) Changes in bacterial community structure in seawater mesocosms differing in their nutrient status. Aquat. Microb. Ecol. 19, 255–267.
  83. CrossRef, ISI
  84. [24]Fuhrman, J.A., Comeau, D.E., Hagstrom, A., Chan, A.M. (1988) Extraction from natural planktonic microorganisms of DNA suitable for molecular biological studies. Appl. Environ. Microbiol. 54, 1426–1429.
  85. Medline, ISI, Chemport
  86. [25]Lane, D.J. (1991) 16S/23S rRNA sequencing. In: Nucleic Acid Techniques in Bacterial Systematics (Stackebrandt, E., Goodfellow, M., Eds.), pp.115–175 John Wiley &amp; Sons, New York.
  87.  
  88. [26]Ferris, M.J., Muyzer, G., Ward, D.M. (1996) Denaturing gradient gel electrophoresis profiles of 16S rRNA-defined populations inhabiting a hot spring microbial mat community. Appl. Environ. Microbiol. 62, 340–346.
  89. Medline, ISI, Chemport
  90. [27]Powell, S.M., Bowman, J.P., Snape, I., Stark, J.S. (2003) Microbial community variation in pristine and polluted nearshore Antarctic sediments. FEMS Microbiol. Ecol. 45, 135–145.
  91. Synergy, ISI, Chemport
  92. [28]Clarke, K.R. and Warwick, R.M. (2001) Change in marine communities: an approach to statistical analysis and interpretation, 2nd ed. Primer-E Ltd., Plymouth, UK. (http://www.pml.ac.uk/primer/)
  93.  
  94. [29]Schiffman, S.S., Reynolds, M.L. (1981) Introduction to Multidimensional Scaling. Theory, Methods and Applications. Academic Press, London, UK.
  95.  
  96. [30]Hall, T.A. (1999) A user-friendly biological sequence alignment editor for Windows 95/98/NT. Nucl. Acids Symp. Ser. 41, pp. 95–98
  97.  
  98. [31]Thompson, J.D., Higgins, D.G., Gibson, T.J. (1994) Clustal-W- improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucl. Acids Res. 22, 4673–4680.
  99. Medline, ISI
  100. [32]Felsenstein, J. (1993) PHYLIP, phylogenetic inference package. http://evolution.genetics.washington.edu/phylip.html
  101.  
  102. [33]Ouverney, C.C., Fuhrman, J.A. (1997) Increase in fluorescence intensity of 16S rRNA in situ hybridization in natural samples treated with chloramphenicol. Appl. Environ. Microbiol. 63, 2735–2740.
  103. Medline, ISI
  104. [34]Bano, N., Hollibaugh, J.T. (2002) Phylogenetic composition of bacterioplankton assemblages from the Arctic Ocean. Appl. Environ. Microbiol. 68, 505–518.
  105. CrossRef, Medline, ISI, Chemport
  106. [35]Simon, M., Glöckner, F.O., Amann, R. (1999) Different community structure and temperature optima of heterotrophic picoplankton in various regions of the Southern Ocean. Aquat. Microb. Ecol. 18, 275–284.
  107. CrossRef, ISI
  108. [36]de Baar, H.J.W., Buma, A.G.J., Nolting, R.F., Cadee, G.C., Jacques, G., Treguer, P.J. (1990) On iron limitation of the Southern-Ocean – experimental-observations in the Weddell and Scotia seas. Mar. Ecol. Prog. Ser 65, 105–122.
  109.  
  110. [37]Timmermans, K.R., van Leeuwe, M.A., de Jong, J.T.M., McKay, R.M.L., Nolting, R.F., Witte, H.J., van Ooyen, J., Swagerman, M.J.W., Kloosterhuis, H., de Baar, H.J.W. (1998) Iron stress in the Pacific region of the Southern Ocean: evidence from enrichment bioassays. Mar. Ecol. Prog. Ser. 166, 27–41.
  111. ISI
  112. [38]Vincent, W.F. (1988) Microbial Ecosystems of Antarctica. Cambridge University Press, Cambridge, UK.
  113.  
  114. [39]Hence, I., Zimmermann, R., Beckman, A., Batman, U.V. (2003) Regional ecosystem dynamics in the ACC: simulations with a three-dimensional ocean-plankton model. J. Mar. Syst. 42, 31–51.
  115.  
  116. [40]Murray, A.E., Wu, K.Y., Moyer, C.L., Karl, D.M., de Long, E.F. (1999) Evidence for circumpolar distribution of planktonic Archaea in the Southern Ocean. Aquat. Microb. Ecol. 18, 263–273.
  117. ISI
  118. [41]Selje, N., Simon, M., Brinkhoff, T. (2004) A newly discovered Roseobacter cluster in temperate and polar oceans. Nature 427, 445–448.
  119. CrossRef, Medline, ISI, Chemport
  120. [42]Long, R.A., Azam, F. (2001) Microscale patchiness of bacterioplankton assemblage richness in seawater. Aquat. Microb. Ecol. 26, 103–113.
  121. CrossRef, ISI
  122. [43]Bowman, J.P., McCammon, S.A., Brown, M.V., Nichols, D.S., McMeekin, T.A. (1997) Diversity and association of psychrophilic bacteria in Antarctic sea ice. Appl. Environ. Microbiol. 63, 3068–3078.
  123. Medline, ISI, Chemport
  124. [44]Bowman, J.P., McCammon, S.A., Brown, J.L., Nichols, P.D., McMeekin, T.A. (1997) Psychroserpens burtonensis gen. nov., sp. nov. and Gelidibacter algens gen. nov., sp. nov., psychrophilic bacteria isolated from Antarctic lacustrine and sea ice habitats. Int. J. Syst. Bacteriol. 47, 670–677.
  125. Medline
  126. [45]Gosink, J.J., Woese, C.R., Staley, J.T. (1998) Polaribacter gen. nov., with three new species, P. irgensii sp. nov., P. franzmannii sp. nov., P. filamentus sp. nov., gas vacuolate polar marine bacteria of the Cytophaga–Flavobacterium–Bacteroides group and reclassification of &quot;Flectobacillus glomeratus&quot; as Polaribacter glomeratus comb. nov. Int. J. Syst. Bacteriol. 48, 223–235.
  127.  
  128. [46]Zubkov, M.V., Fuchs, B.M., Archer, S.D., Kiene, R.P., Amann, R., Burkill, P.H. (2002) A population of the alpha-proteobacteria dominates the bacterioplankton and dimethylsulphoniopropionate uptake after an algal bloom in the North Sea. Deep-sea Res. II, Top. Stud. Oceanogr. 49, 3017–3038.
  129. ISI
  130. [47]Beja, O., Suzuki, M.T., Koonin, E.V., Aravind, L., Hadd, A., Nguyen, L.P., Villacorta, R., Amjadi, M., Garrigues, C., Jovanovich, S.B., Feldman, R.A., DeLong, E. (2000) Construction and analysis of bacterial artificial chromosome libraries from a marine microbial assemblage. Environ. Microbiol. 2, 516–529.
  131. Synergy, Medline, ISI, Chemport
  132. [48]Casamayor, E.O., Schafer, H., Baneras, L., Pedros-Alio, C., Muyzer, G. (2000) Identification of and spatio-temporal differences between microbial assemblages from two neighboring sulfurous lakes: Comparison by microscopy and denaturing gradient gel electrophoresis. Appl. Environ. Microbiol. 66, 499–508.
  133. CrossRef, Medline, ISI, Chemport
  134. [49]Deacon, G.E.R. (1984) The Antarctic Circumpolar Ocean. Cambridge University Press, Cambridge.
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  263.     <h1 class="ep_tm_pagetitle">Ecological and biogeographic relationships of class Flavobacteria in the Southern Ocean</h1>
  264.     <p style="margin-bottom: 1em" class="not_ep_block"><span class="person_name">Abell, G.C.J.</span> and <span class="person_name">Bowman, John P.</span> (2005) <xhtml:em>Ecological and biogeographic relationships of class Flavobacteria in the Southern Ocean.</xhtml:em> FEMS Microbiology Ecology, 51 . pp. 265-277.</p><p style="margin-bottom: 1em" class="not_ep_block"></p><table style="margin-bottom: 1em" class="not_ep_block"><tr><td valign="top" style="text-align:center"><a href="http://eprints.utas.edu.au/2129/1/Abell_Bowman_2005a.pdf"><img alt="[img]" src="http://eprints.utas.edu.au/style/images/fileicons/application_pdf.png" class="ep_doc_icon" border="0" /></a></td><td valign="top"><a href="http://eprints.utas.edu.au/2129/1/Abell_Bowman_2005a.pdf"><span class="ep_document_citation">PDF</span></a> - Full text restricted - Requires a PDF viewer<br />634Kb</td><td><form method="get" accept-charset="utf-8" action="http://eprints.utas.edu.au/cgi/request_doc"><input accept-charset="utf-8" value="2667" name="docid" type="hidden" /><div class=""><input value="Request a copy" name="_action_null" class="ep_form_action_button" onclick="return EPJS_button_pushed( '_action_null' )" type="submit" /> </div></form></td></tr></table><p style="margin-bottom: 1em" class="not_ep_block">Official URL: <a href="http://dx.doi.org/10.1016/j.femsec.2004.09.001">http://dx.doi.org/10.1016/j.femsec.2004.09.001</a></p><div class="not_ep_block"><h2>Abstract</h2><p style="padding-bottom: 16px; text-align: left; margin: 1em auto 0em auto">The abundance, spatial distribution and diversity of class Flavobacteria were investigated in the Southern Ocean euphotic zone across a latitudinal transect and in the ice pack off Eastern Antarctica. Surface seawater samples filter-fractionated into 0.8 μm particulate and 0.2 m planktonic fractions were investigated with different molecular techniques. The abundance of particle-associated Flavobacteria, ascertained with real-time PCR and DGGE band analysis using Flavobacteria-specific primers, was found to be significantly higher in Polar Front Zone (PFZ) and Antarctic Zone (AZ) water samples than in nutrient limited Temperate Zone (TZ) and Sub-Antarctic Zone (SAZ) waters. Abundance of particle-associated Flavobacteria correlated positively with seawater chlorophyll a and nutrient concentrations, suggesting that increased Flavobacteria abundance may relate to enhanced primary production in the PFZ and AZ. This is supported by comparison of DGGE profiles that demonstrated significant differences in the total Flavobacteria community structure and 16S rRNA gene diversity between samples from the PFZ and AZ and those from TZ and SAZ. Sequence analysis revealed a broad diversity amongst class Flavobacteria in the Southern Ocean with several Flavobacteria clades detected in PFZ and AZ waters not detected in TZ and SAZ waters that putatively represent psychrophilic taxa. Sequence data included a large, so far uncultivated, cosmopolitan phylogenetic clade ("DE cluster 2") that is distributed throughout the Southern Ocean.&#13;
  265. &#13;
  266. </p></div><table style="margin-bottom: 1em" cellpadding="3" class="not_ep_block" border="0"><tr><th valign="top" class="ep_row">Item Type:</th><td valign="top" class="ep_row">Article</td></tr><tr><th valign="top" class="ep_row">Additional Information:</th><td valign="top" class="ep_row">The definitive version is available at http://www.sciencedirect.com/&#13;
  267. </td></tr><tr><th valign="top" class="ep_row">Keywords:</th><td valign="top" class="ep_row">Southern Ocean; DGGE; Flavobacteria; Cytophaga–Flavobacterium–Bacteroides group; Biogeography; Psychrophilic bacteria</td></tr><tr><th valign="top" class="ep_row">Subjects:</th><td valign="top" class="ep_row"><a href="http://eprints.utas.edu.au/view/subjects/270307.html">270000 Biological Sciences &gt; 270300 Microbiology &gt; 270307 Microbial Ecology</a></td></tr><tr><th valign="top" class="ep_row">ID Code:</th><td valign="top" class="ep_row">2129</td></tr><tr><th valign="top" class="ep_row">Deposited By:</th><td valign="top" class="ep_row"><span class="ep_name_citation"><span class="person_name">Assoc. Prof. John P. Bowman</span></span></td></tr><tr><th valign="top" class="ep_row">Deposited On:</th><td valign="top" class="ep_row">15 Oct 2007 10:29</td></tr><tr><th valign="top" class="ep_row">Last Modified:</th><td valign="top" class="ep_row">09 Jan 2008 02:30</td></tr><tr><th valign="top" class="ep_row">ePrint Statistics:</th><td valign="top" class="ep_row"><a target="ePrintStats" href="/es/index.php?action=show_detail_eprint;id=2129;">View statistics for this ePrint</a></td></tr></table><p align="right">Repository Staff Only: <a href="http://eprints.utas.edu.au/cgi/users/home?screen=EPrint::View&amp;eprintid=2129">item control page</a></p>
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