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  5. <title>UTas ePrints - Vertical migration of the toxic dinoflagellate Gymnodinium catenatum under different concentrations of nutrients and humic substances in culture</title>
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  13. <meta content="Doblin, Marina A." name="eprints.creators_name" />
  14. <meta content="Thompson, Peter A." name="eprints.creators_name" />
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  17. <meta content="Blackburn, Susan I." name="eprints.creators_name" />
  18. <meta content="Hallegraeff, Gustaaf M." name="eprints.creators_name" />
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  29. <meta content="Vertical migration of the toxic dinoflagellate Gymnodinium
  30. catenatum under different concentrations of nutrients and
  31. humic substances in culture" name="eprints.title" />
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  33. <meta content="270702" name="eprints.subjects" />
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  37. <meta content="CDOM; Gymnodinium catenatum; Humic substances; Nitrogen; N-stratified; Vertical migration" name="eprints.keywords" />
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  40. <meta content="Vertical migration behaviour by the chainforming dinoflagellate Gymnodinium catenatum Graham was investigated using
  41. vertically-stratified laboratory columns. Under surface nutrient-deplete conditions, with nutrients added only at depth, 100% of
  42. cells underwent vertical migration (VM), starting downwards migration 3 h before the end of the light period and beginning
  43. upwards migration 3 h before the start of the light period. Cells in nutrient-replete columns showed no VM, but they were more
  44. dispersed in the upper layer during the dark compared to the light period. When surface layers (S) were nitrate-deplete (N) and
  45. enriched with humic substances (H) contained in Huon River water and bottom waters (B) were nutrient replete (R) (SHNBR), the
  46. pattern ofVMwas altered—50% of cells underwent migration and 50% remained at the pycnocline. In columns with nitrate-replete
  47. and humic-enriched surface layers (SHRBR), most cells underwent VM, while 30% remained at the surface. Cells in SHNBR
  48. columns showed increased N quotas and intra-cellular nitrate concentrations after 4 days, indicating nitrate uptake by G. catenatum
  49. in bottom layers. The concomitant increase in particulate organic nitrogen (PON) with the decrease in external nitrate
  50. concentrations in bottom layers provide convincing evidence that VM by G. catenatum facilitates nutrient retrieval at depth.
  51. However, addition of humic substances (a potential source of organic nitrogen) to surface layers did not ameliorate G. catenatum N
  52. depletion sufficiently to preclude the need for NO3 uptake at depth. Furthermore, there was no detectable pattern of increasing
  53. carbon (C) quota during the day (photosynthate accumulation) or increasing N quota during the night (nitrate assimilation). Toxic
  54. dinoflagellate G. catenatum blooms are commonly associated with nitrate depletion in surface waters in south-east Tasmanian
  55. waters (Australia). Therefore, vertical migration, facilitating N uptake at depth, could play an important role in this organism’s
  56. ecological strategy, enabling it to exploit environments where light and nutrients are vertically separated" name="eprints.abstract" />
  57. <meta content="2006" name="eprints.date" />
  58. <meta content="published" name="eprints.date_type" />
  59. <meta content="Harmful Algae" name="eprints.publication" />
  60. <meta content="5" name="eprints.volume" />
  61. <meta content="6" name="eprints.number" />
  62. <meta content="665-677" name="eprints.pagerange" />
  63. <meta content="10.1016/j.hal.2006.02.002" name="eprints.id_number" />
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  65. <meta content="1568-9883" name="eprints.issn" />
  66. <meta content="http://dx.doi.org/10.1016/j.hal.2006.02.002" name="eprints.official_url" />
  67. <meta content="Armstrong, P., Thompson, P.A., Bolch, C.J., Blackburn, S.I., 2004.
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  73. Gymnodinium catenatum from Tasmania, Australia. J. Phycol.
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  243. behaviour model: response to internal biochemical cues. Ecol.
  244. Model. 134, 59–" name="eprints.referencetext" />
  245. <meta content="Doblin, Marina A. and Thompson, Peter A. and Revill, Andrew T. and Bulter, Edward C.V. and Blackburn, Susan I. and Hallegraeff, Gustaaf M. (2006) Vertical migration of the toxic dinoflagellate Gymnodinium catenatum under different concentrations of nutrients and humic substances in culture. Harmful Algae, 5 (6). pp. 665-677. ISSN 1568-9883" name="eprints.citation" />
  246. <meta content="http://eprints.utas.edu.au/2498/1/Vertical_migration_of_the_tonic.pdf" name="eprints.document_url" />
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  248. <meta content="Vertical migration of the toxic dinoflagellate Gymnodinium
  249. catenatum under different concentrations of nutrients and
  250. humic substances in culture" name="DC.title" />
  251. <meta content="Doblin, Marina A." name="DC.creator" />
  252. <meta content="Thompson, Peter A." name="DC.creator" />
  253. <meta content="Revill, Andrew T." name="DC.creator" />
  254. <meta content="Bulter, Edward C.V." name="DC.creator" />
  255. <meta content="Blackburn, Susan I." name="DC.creator" />
  256. <meta content="Hallegraeff, Gustaaf M." name="DC.creator" />
  257. <meta content="270702 Marine and Estuarine Ecology (incl. Marine Ichthyology)" name="DC.subject" />
  258. <meta content="270700 Ecology and Evolution" name="DC.subject" />
  259. <meta content="260400 Oceanography" name="DC.subject" />
  260. <meta content="Vertical migration behaviour by the chainforming dinoflagellate Gymnodinium catenatum Graham was investigated using
  261. vertically-stratified laboratory columns. Under surface nutrient-deplete conditions, with nutrients added only at depth, 100% of
  262. cells underwent vertical migration (VM), starting downwards migration 3 h before the end of the light period and beginning
  263. upwards migration 3 h before the start of the light period. Cells in nutrient-replete columns showed no VM, but they were more
  264. dispersed in the upper layer during the dark compared to the light period. When surface layers (S) were nitrate-deplete (N) and
  265. enriched with humic substances (H) contained in Huon River water and bottom waters (B) were nutrient replete (R) (SHNBR), the
  266. pattern ofVMwas altered—50% of cells underwent migration and 50% remained at the pycnocline. In columns with nitrate-replete
  267. and humic-enriched surface layers (SHRBR), most cells underwent VM, while 30% remained at the surface. Cells in SHNBR
  268. columns showed increased N quotas and intra-cellular nitrate concentrations after 4 days, indicating nitrate uptake by G. catenatum
  269. in bottom layers. The concomitant increase in particulate organic nitrogen (PON) with the decrease in external nitrate
  270. concentrations in bottom layers provide convincing evidence that VM by G. catenatum facilitates nutrient retrieval at depth.
  271. However, addition of humic substances (a potential source of organic nitrogen) to surface layers did not ameliorate G. catenatum N
  272. depletion sufficiently to preclude the need for NO3 uptake at depth. Furthermore, there was no detectable pattern of increasing
  273. carbon (C) quota during the day (photosynthate accumulation) or increasing N quota during the night (nitrate assimilation). Toxic
  274. dinoflagellate G. catenatum blooms are commonly associated with nitrate depletion in surface waters in south-east Tasmanian
  275. waters (Australia). Therefore, vertical migration, facilitating N uptake at depth, could play an important role in this organism’s
  276. ecological strategy, enabling it to exploit environments where light and nutrients are vertically separated" name="DC.description" />
  277. <meta content="2006" name="DC.date" />
  278. <meta content="Article" name="DC.type" />
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  283. <meta content="Doblin, Marina A. and Thompson, Peter A. and Revill, Andrew T. and Bulter, Edward C.V. and Blackburn, Susan I. and Hallegraeff, Gustaaf M. (2006) Vertical migration of the toxic dinoflagellate Gymnodinium catenatum under different concentrations of nutrients and humic substances in culture. Harmful Algae, 5 (6). pp. 665-677. ISSN 1568-9883" name="DC.identifier" />
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  388. <h1 class="ep_tm_pagetitle">Vertical migration of the toxic dinoflagellate Gymnodinium catenatum under different concentrations of nutrients and humic substances in culture</h1>
  389. <p style="margin-bottom: 1em" class="not_ep_block"><span class="person_name">Doblin, Marina A.</span> and <span class="person_name">Thompson, Peter A.</span> and <span class="person_name">Revill, Andrew T.</span> and <span class="person_name">Bulter, Edward C.V.</span> and <span class="person_name">Blackburn, Susan I.</span> and <span class="person_name">Hallegraeff, Gustaaf M.</span> (2006) <xhtml:em>Vertical migration of the toxic dinoflagellate Gymnodinium catenatum under different concentrations of nutrients and humic substances in culture.</xhtml:em> Harmful Algae, 5 (6). pp. 665-677. ISSN 1568-9883</p><p style="margin-bottom: 1em" class="not_ep_block"></p><table style="margin-bottom: 1em" class="not_ep_block"><tr><td valign="top" style="text-align:center"><a href="http://eprints.utas.edu.au/2498/1/Vertical_migration_of_the_tonic.pdf"><img alt="[img]" src="http://eprints.utas.edu.au/style/images/fileicons/application_pdf.png" class="ep_doc_icon" border="0" /></a></td><td valign="top"><a href="http://eprints.utas.edu.au/2498/1/Vertical_migration_of_the_tonic.pdf"><span class="ep_document_citation">PDF</span></a> - Full text restricted - Requires a PDF viewer<br />872Kb</td><td><form method="get" accept-charset="utf-8" action="http://eprints.utas.edu.au/cgi/request_doc"><input accept-charset="utf-8" value="3286" name="docid" type="hidden" /><div class=""><input value="Request a copy" name="_action_null" class="ep_form_action_button" onclick="return EPJS_button_pushed( '_action_null' )" type="submit" /> </div></form></td></tr></table><p style="margin-bottom: 1em" class="not_ep_block">Official URL: <a href="http://dx.doi.org/10.1016/j.hal.2006.02.002">http://dx.doi.org/10.1016/j.hal.2006.02.002</a></p><div class="not_ep_block"><h2>Abstract</h2><p style="padding-bottom: 16px; text-align: left; margin: 1em auto 0em auto">Vertical migration behaviour by the chainforming dinoflagellate Gymnodinium catenatum Graham was investigated using&#13;
  390. vertically-stratified laboratory columns. Under surface nutrient-deplete conditions, with nutrients added only at depth, 100% of&#13;
  391. cells underwent vertical migration (VM), starting downwards migration 3 h before the end of the light period and beginning&#13;
  392. upwards migration 3 h before the start of the light period. Cells in nutrient-replete columns showed no VM, but they were more&#13;
  393. dispersed in the upper layer during the dark compared to the light period. When surface layers (S) were nitrate-deplete (N) and&#13;
  394. enriched with humic substances (H) contained in Huon River water and bottom waters (B) were nutrient replete (R) (SHNBR), the&#13;
  395. pattern ofVMwas altered—50% of cells underwent migration and 50% remained at the pycnocline. In columns with nitrate-replete&#13;
  396. and humic-enriched surface layers (SHRBR), most cells underwent VM, while 30% remained at the surface. Cells in SHNBR&#13;
  397. columns showed increased N quotas and intra-cellular nitrate concentrations after 4 days, indicating nitrate uptake by G. catenatum&#13;
  398. in bottom layers. The concomitant increase in particulate organic nitrogen (PON) with the decrease in external nitrate&#13;
  399. concentrations in bottom layers provide convincing evidence that VM by G. catenatum facilitates nutrient retrieval at depth.&#13;
  400. However, addition of humic substances (a potential source of organic nitrogen) to surface layers did not ameliorate G. catenatum N&#13;
  401. depletion sufficiently to preclude the need for NO3 uptake at depth. Furthermore, there was no detectable pattern of increasing&#13;
  402. carbon (C) quota during the day (photosynthate accumulation) or increasing N quota during the night (nitrate assimilation). Toxic&#13;
  403. dinoflagellate G. catenatum blooms are commonly associated with nitrate depletion in surface waters in south-east Tasmanian&#13;
  404. waters (Australia). Therefore, vertical migration, facilitating N uptake at depth, could play an important role in this organism’s&#13;
  405. ecological strategy, enabling it to exploit environments where light and nutrients are vertically separated</p></div><table style="margin-bottom: 1em" cellpadding="3" class="not_ep_block" border="0"><tr><th valign="top" class="ep_row">Item Type:</th><td valign="top" class="ep_row">Article</td></tr><tr><th valign="top" class="ep_row">Additional Information:</th><td valign="top" class="ep_row">The definitive version is available at http://www.sciencedirect.com&#13;
  406. </td></tr><tr><th valign="top" class="ep_row">Keywords:</th><td valign="top" class="ep_row">CDOM; Gymnodinium catenatum; Humic substances; Nitrogen; N-stratified; Vertical migration</td></tr><tr><th valign="top" class="ep_row">Subjects:</th><td valign="top" class="ep_row"><a href="http://eprints.utas.edu.au/view/subjects/270702.html">270000 Biological Sciences &gt; 270700 Ecology and Evolution &gt; 270702 Marine and Estuarine Ecology (incl. Marine Ichthyology)</a><br /><a href="http://eprints.utas.edu.au/view/subjects/270700.html">270000 Biological Sciences &gt; 270700 Ecology and Evolution</a><br /><a href="http://eprints.utas.edu.au/view/subjects/260400.html">260000 Earth Sciences &gt; 260400 Oceanography</a></td></tr><tr><th valign="top" class="ep_row">ID Code:</th><td valign="top" class="ep_row">2498</td></tr><tr><th valign="top" class="ep_row">Deposited By:</th><td valign="top" class="ep_row"><span class="ep_name_citation"><span class="person_name">Scholarly Publications Librarian</span></span></td></tr><tr><th valign="top" class="ep_row">Deposited On:</th><td valign="top" class="ep_row">21 Nov 2007 09:24</td></tr><tr><th valign="top" class="ep_row">Last Modified:</th><td valign="top" class="ep_row">09 Jan 2008 02:30</td></tr><tr><th valign="top" class="ep_row">ePrint Statistics:</th><td valign="top" class="ep_row"><a target="ePrintStats" href="/es/index.php?action=show_detail_eprint;id=2498;">View statistics for this ePrint</a></td></tr></table><p align="right">Repository Staff Only: <a href="http://eprints.utas.edu.au/cgi/users/home?screen=EPrint::View&amp;eprintid=2498">item control page</a></p>
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