- <!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN"
- "http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd">
- <html>
- <head>
- <title>UTas ePrints - Vertical migration of the toxic dinoflagellate Gymnodinium catenatum under different concentrations of nutrients and humic substances in culture</title>
- <script type="text/javascript" src="http://eprints.utas.edu.au/javascript/auto.js"><!-- padder --></script>
- <style type="text/css" media="screen">@import url(http://eprints.utas.edu.au/style/auto.css);</style>
- <style type="text/css" media="print">@import url(http://eprints.utas.edu.au/style/print.css);</style>
- <link rel="icon" href="/images/eprints/favicon.ico" type="image/x-icon" />
- <link rel="shortcut icon" href="/images/eprints/favicon.ico" type="image/x-icon" />
- <link rel="Top" href="http://eprints.utas.edu.au/" />
- <link rel="Search" href="http://eprints.utas.edu.au/cgi/search" />
- <meta content="Doblin, Marina A." name="eprints.creators_name" />
- <meta content="Thompson, Peter A." name="eprints.creators_name" />
- <meta content="Revill, Andrew T." name="eprints.creators_name" />
- <meta content="Bulter, Edward C.V." name="eprints.creators_name" />
- <meta content="Blackburn, Susan I." name="eprints.creators_name" />
- <meta content="Hallegraeff, Gustaaf M." name="eprints.creators_name" />
- <meta content="" name="eprints.creators_id" />
- <meta content="" name="eprints.creators_id" />
- <meta content="" name="eprints.creators_id" />
- <meta content="" name="eprints.creators_id" />
- <meta content="" name="eprints.creators_id" />
- <meta content="Hallegraeff@utas.edu.au" name="eprints.creators_id" />
- <meta content="article" name="eprints.type" />
- <meta content="2007-11-20 22:24:26" name="eprints.datestamp" />
- <meta content="2008-01-08 15:30:00" name="eprints.lastmod" />
- <meta content="show" name="eprints.metadata_visibility" />
- <meta content="Vertical migration of the toxic dinoflagellate Gymnodinium
- catenatum under different concentrations of nutrients and
- humic substances in culture" name="eprints.title" />
- <meta content="pub" name="eprints.ispublished" />
- <meta content="270702" name="eprints.subjects" />
- <meta content="270700" name="eprints.subjects" />
- <meta content="260400" name="eprints.subjects" />
- <meta content="restricted" name="eprints.full_text_status" />
- <meta content="CDOM; Gymnodinium catenatum; Humic substances; Nitrogen; N-stratified; Vertical migration" name="eprints.keywords" />
- <meta content="The definitive version is available at http://www.sciencedirect.com
- " name="eprints.note" />
- <meta content="Vertical migration behaviour by the chainforming dinoflagellate Gymnodinium catenatum Graham was investigated using
- vertically-stratified laboratory columns. Under surface nutrient-deplete conditions, with nutrients added only at depth, 100% of
- cells underwent vertical migration (VM), starting downwards migration 3 h before the end of the light period and beginning
- upwards migration 3 h before the start of the light period. Cells in nutrient-replete columns showed no VM, but they were more
- dispersed in the upper layer during the dark compared to the light period. When surface layers (S) were nitrate-deplete (N) and
- enriched with humic substances (H) contained in Huon River water and bottom waters (B) were nutrient replete (R) (SHNBR), the
- pattern ofVMwas altered—50% of cells underwent migration and 50% remained at the pycnocline. In columns with nitrate-replete
- and humic-enriched surface layers (SHRBR), most cells underwent VM, while 30% remained at the surface. Cells in SHNBR
- columns showed increased N quotas and intra-cellular nitrate concentrations after 4 days, indicating nitrate uptake by G. catenatum
- in bottom layers. The concomitant increase in particulate organic nitrogen (PON) with the decrease in external nitrate
- concentrations in bottom layers provide convincing evidence that VM by G. catenatum facilitates nutrient retrieval at depth.
- However, addition of humic substances (a potential source of organic nitrogen) to surface layers did not ameliorate G. catenatum N
- depletion sufficiently to preclude the need for NO3 uptake at depth. Furthermore, there was no detectable pattern of increasing
- carbon (C) quota during the day (photosynthate accumulation) or increasing N quota during the night (nitrate assimilation). Toxic
- dinoflagellate G. catenatum blooms are commonly associated with nitrate depletion in surface waters in south-east Tasmanian
- waters (Australia). Therefore, vertical migration, facilitating N uptake at depth, could play an important role in this organism’s
- ecological strategy, enabling it to exploit environments where light and nutrients are vertically separated" name="eprints.abstract" />
- <meta content="2006" name="eprints.date" />
- <meta content="published" name="eprints.date_type" />
- <meta content="Harmful Algae" name="eprints.publication" />
- <meta content="5" name="eprints.volume" />
- <meta content="6" name="eprints.number" />
- <meta content="665-677" name="eprints.pagerange" />
- <meta content="10.1016/j.hal.2006.02.002" name="eprints.id_number" />
- <meta content="TRUE" name="eprints.refereed" />
- <meta content="1568-9883" name="eprints.issn" />
- <meta content="http://dx.doi.org/10.1016/j.hal.2006.02.002" name="eprints.official_url" />
- <meta content="Armstrong, P., Thompson, P.A., Bolch, C.J., Blackburn, S.I., 2004.
- Nutrient dynamics and phytoplankton blooms in the Huon Estuary.
- In: Abstracts Australasian Aquaculture Convention, Sydney,
- 26–29 September, p. 65.
- Blackburn, S.I., Hallegraeff, G.M., Bolch, C.J., 1989. Vegetative
- reproduction and sexual life cycle of the toxic dinoflagellate
- Gymnodinium catenatum from Tasmania, Australia. J. Phycol.
- 25, 577–590.
- Carlsson, P., Grane´li, E., 1998. Utilisation of dissolved organic matter
- (DOM) by phytoplankton, including harmful species. In: Anderson,
- D.M., Cembella, A.D., Hallegraeff, G.M. (Eds.), The Physiological
- Ecology of Harmful Algal Blooms. Springer Verlag,
- Berlin, pp. 509–524.
- Carmona, R., Vergara, J.J., Perezllorens, J.L., Figueroa, F.L., Niell,
- F.X., 1996. Photosynthetic acclimation and biochemical responses
- of Gelidium sesquipedale culture in chemostats under different
- qualities of light. Mar. Biol. 127, 25–34.
- Cook, P.L.M., Revill, A.T., Butler, E.C.V., Eyre, B.D., 2004. Carbon
- and nitrogen cycling on intertidal mudflats of a temperate Australian
- estuary. II Nitrogen cycling. Mar. Ecol. Prog. Ser. 280, 39–
- 54.
- Crawford, D.W., Purdie, D.A., 1992. Evidence for avoidance of
- flushing from an estuary by a planktonic, phototrophic ciliate.
- Mar. Ecol. Prog. Ser. 79, 259–265.
- Cullen, J.J., 1985. Diel vertical migration by dinoflagellates: roles of
- carbohydrate metabolism and behavioral flexibility. Contr. Mar.
- Sci. 27 (Suppl.), 135–152.
- Doblin, M.A., Blackburn, S.I., Hallegraeff, G.M., 1999. Comparative
- study of selenium requirement of three phytoplankton species:
- Gymnodinium catenatum, Alexandrium minutum (Dinophyceae)
- and Chaetoceros cf. tenuissimus (Bacillariophyceae). J. Plankton
- Res. 21 (6), 1153–1169.
- Doblin, M.A., Legrand, C., Carlsson, P., Hummert, C., Grane´li, E.,
- Hallegraeff,G.M., 2001. Uptake of humic substances by the toxic
- dinoflagellate Alexandrium catenella. In: Hallegraeff, G.M.,
- Blackburn, S.I., Bolch, C.J., Lewis, R.J. (Eds.), Proceedings
- of the Ninth International Conference on Harmful Algal Blooms,
- Intergovernmental Oceanographic Commission of UNESCO,
- pp. 336–339.
- Dortch, Q., Clayton, J.R., Thoresen, S.S., Ahmed, S.I., 1984. Species
- differences in accumulation of nitrogen pools in phytoplankton.
- Mar. Biol. 81, 237–250.
- Dortch, Q., Clayton Jr., J.R., Thoresen, S.S., Bressler, S.L., Ahmed,
- S.I., 1982. Response of marine phytoplankton to nitrogen deficiency:
- decreased nitrate uptake versus enhanced ammonium
- uptake. Mar. Biol. 70, 13–19.
- Eppley, R.W., Holm-Hansen, O., Strickland, J.D.H., 1968. Some
- observations on the vertical migration of dinoflagellates. J. Phycol.
- 4, 333–340.
- Fermı´n, E.G., Figueiras, F.G., Arbones, B., Villarino, M.L., 1996.
- Short-time scale development of a Gymnodinium catenatum
- population in the Ria de Vigo (NW Spain). J. Phycol. 32, 212–221.
- Flynn, K.J., Flynn, K., John, E.H., Reguera, B., Reyero, M.I., Franco,
- J.M., 1996. Changes in toxins, intracellular and dissolved free
- amino acids of the toxic dinoflagellate Gymnodinium catenatum in
- response to changes in inorganic nutrients and salinity. J. Plankton
- Res. 18, 2093–2111.
- Fraga, F., Pe´rez, F.F., Figueiras, F.G., Rı´os, A.F., 1992. Stoichiometric
- variations of N, P, C and O2 during a Gymnodinium catenatum red
- tide and their interpretation. Mar. Ecol. Prog. Ser. 87, 123–134.
- Fraga, S., Gallager, S.M., Anderson, D.M., 1989. Chain-forming
- dinoflagellates: an adaptation to red tides. In: Okaichi, T., An
- derson, D.M., Nemoto, T. (Eds.), Red Tides: Biology, Environmental
- Science and Toxicology. Elsevier Science, New York, pp.
- 281–284.
- Fraga, S., Reguera, B., Bravo, I., 1990. Gymnodinium catenatum
- bloom formation in the Spanish Rias. In: Grane´li, E., Sundstro¨m,
- B., Edler, L., Anderson, D.M. (Eds.), Toxic Marine Phytoplankton.
- Elsevier Science, New York, pp. 149–154.
- Fritz, L., Stringher, C.G., Colepicolo, P., 1996. Immunolocalisation of
- nitrate reductase in the marine dinoflagellate Gonyaulax polyedra
- (Pyrrophyta). J. Phycol. 32, 632–637.
- Ganf, G.G., Oliver, R.L., 1982. Vertical separation of light and
- available nutrients as a factor causing replacement of green algae
- by blue-green algae in the plankton of a stratified lake. J. Ecol. 70,
- 829–844.
- George, D.G., Heaney, S.I., 1978. Factors influencing the spatial
- distribution of phytoplankton in a small productive lake. J. Ecol.
- 66, 133–155.
- Grane´li, E., Johansson, N., Panosso, R., 1998. Cellular toxin contents
- in relation to nutrient conditions for different groups of phycotoxins.
- In: Reguera, B., Blanco, J., Ferna´ndez, M.L., Wyatt, T.
- (Eds.), Harmful Algae. Xunta de Galicia and Intergovernmental
- Oceanographic Commission of UNESCO, pp. 321–324.
- Hallegraeff, G.M., McCausland, M.A., Brown, R.K., 1995. Early
- warning of toxic dinoflagellate blooms of Gymnodinium catenatum
- in southern Tasmanian waters. J. Plankton Res. 17, 1163–
- 1176.
- Harris, G.P., Heaney, S.I., Talling, J.F., 1979. Physiological and
- environmental constraints in the ecology of the planktonic dinoflagellate
- Ceratium hirundinella. Freshwater Biol. 9, 413–428.
- Heil, C.A., 2003. Assessing the bioavailability and uptake of dissolved
- humic substances by the HAB species Karenia brevis using
- radioisotopic techniques. In: Second Symposium on Harmful
- Marine Algae in the US, 9–13 December 2003, Woods Hole,
- Massachusetts, USA.
- Huon Estuary Study Team, 2000. Huon Estuary Study, Environmental
- research for integrated catchment management and aquaculture.
- Final report to FRDC. Project 96/284, CSIRO Division Marine
- Research, Marine Laboratories, Hobart, Tasmania, Australia.
- Kamykowski, D., 1981. Laboratory experiments on the diurnal vertical
- migration of marine dinoflagellates through temperature
- gradients. Mar. Biol. 62, 81–89.
- Kamykowski, D., Milligan, E.J., Reed, R.E., Liu,W., 1999. Geotaxis/
- phototaxis and biochemical patterns in Heterocapsa (Cachonina)
- illdefina (Dinophyceae) during diel vertical migrations. J. Phycol.
- 35 (6 Suppl.), 1397–1403.
- Kirk, J.T.O., 1994. Light and Photosynthesis in Aquatic Ecosystems.
- Cambridge University Press, Cambridge.
- Langdon, C., 1987. On the causes of interspecific differences in the
- growth-irradiance relationship for phytoplankton. Part I. A comparative
- study of the growth-irradiance relationship of three marine
- phytoplankton species: Skeletonema costatum, Olisthodiscus luteus
- and Gonyaulax tamarensis. J. Plankton. Res. 9, 459–482.
- Lieberman, O.S., Shilo, M., van Rijn, J., 1994. The physiological
- ecology of a freshwater dinoflagellate bloom population: vertical
- migration, nitrogen limitation, and nutrient uptake kinetics. J.
- Phycol. 30, 964–971.
- Loeblich, A.R., 1975. A seawater medium for dinoflagellates and the
- nutrition of Cachonina niei. J. Phycol. 11, 80–86.
- MacIntyre, J.G., Cullen, J.J., Cembella, A.D., 1997. Vertical migration,
- nutrition and toxicity in the dinoflagellate Alexandrium
- tamarense. Mar. Ecol. Prog. Ser. 148, 201–216.
- Mulholland, M.R., Gobler, C.J., Lee, C., 2002. Peptide hydrolysis,
- amino acid oxidation, and nitrogen uptake in communities seasonally
- dominated by Aureococcus anophagefferens. Limnol.
- Oceanogr. 47 (4), 1094–1108.
- Ogata, T., Kodama, M., Ishimaru, T., 1989. Effect of water temperature
- and light intensity on growth rate and toxin production of
- dinoflagellates. In: Okaichi, T., Anderson, D.M., Nemoto, T.
- (Eds.), Red Tides: Biology, Environmental Science, and Toxicology.
- Elsevier, New York, pp. 423–426.
- Oshima, Y., Blackburn, S.I., Hallegraeff, G.M., 1993. Comparative
- study on paralytic shellfish toxin profiles of the dinoflagellate
- Gymnodinium catenatum from three different countries. Mar. Biol.
- 116, 471–476.
- Reguera, B., Oshima, Y., 1990. Response of Gymnodinium catenatum
- to increasing levels of nitrate: growth patterns and toxicity.
- In: Grane´li, E., Sundstro¨m, B., Edler, L., Anderson, D.M.
- (Eds.), Toxic Marine Phytoplankton. Elsevier, New York, pp.
- 316–319.
- Rosso, A.L., Azam, F., 1987. Proteolytic activity in coastal oceanic
- waters: depth distribution and relationship to bacterial populations.
- Mar. Ecol. Prog. Ser. 41, 231–240.
- Sanz, A.P., Morenovivian, C., Maldonado, J.M., Gonzalezfontes, A.,
- 1995. Effect of a constant supply of different nitrogen sources on
- protein and carbohydrate content and enzyme activities of Anabaena
- variabilis cells. Physiol. Plant. 95, 39–44.
- Stich, H.-B., Lampert,W., 1981. Predator evasion as an explanation of
- diurnal vertical migration by zooplankton. Nature 293, 396–399.
- Thompson, P.A., Levasseur, M.E., Harrison, P.J., 1989. Light-limited
- growth on ammonium versus nitrate: what is the advantage for
- marine phytoplankton? Limnol. Oceanogr. 34, 1014–1024.
- Thoresen, S.S., Dortch, Q., Ahmed, S.I., 1982. Comparison of methods
- for extracting intracellular pools of inorganic nitrogen from
- marine phytoplankton. J. Plankton Res. 4, 695–704.
- US EPA, 1992. In vitro determination of chlorophyll a and phaeophytin
- a in marine and freshwater phytoplankton by fluorescence.
- In: Methods for the Determination of Chemical Substances in
- Marine and Estuarine Environmental Samples, U.S. Environmental
- Protection Agency, Cincinnati.
- Villareal, T.A., Lipschultz, F., 1995. Internal nitrate concentrations in
- single cells of large phytoplankton from the Sargasso Sea. J.
- Phycol. 31, 689–696.
- Watanabe, M., Kohata, K., Kunugi, M., 1988. Phosphate accumulation
- and metabolism by Heterosigma akashiwo (Raphidophyceae)
- during diel vertical migration in a stratified microcosm. J. Phycol.
- 24, 22–28.
- Watanabe, M., Kohata, K., Kimura, T., 1991. Diel vertical migration
- and nocturnal uptake of nutrients by Chattonella antiqua under
- stable stratification. Limnol. Oceanogr. 36, 593–602.
- Yamamoto, T., Oh, S.J., Kataoka, Y., 2002. Effects of temperature,
- salinity and irradiance on the growth of the toxic dinoflagellate
- Gymnodinium catenatum (Dinophyceae) isolated from Hiroshima
- Bay, Japan. Fish. Sci. 68 (2), 356–363.
- Yamazaki, A.K., Kamykowski, D., 2000. A dinoflagellate adaptive
- behaviour model: response to internal biochemical cues. Ecol.
- Model. 134, 59–" name="eprints.referencetext" />
- <meta content="Doblin, Marina A. and Thompson, Peter A. and Revill, Andrew T. and Bulter, Edward C.V. and Blackburn, Susan I. and Hallegraeff, Gustaaf M. (2006) Vertical migration of the toxic dinoflagellate Gymnodinium catenatum under different concentrations of nutrients and humic substances in culture. Harmful Algae, 5 (6). pp. 665-677. ISSN 1568-9883" name="eprints.citation" />
- <meta content="http://eprints.utas.edu.au/2498/1/Vertical_migration_of_the_tonic.pdf" name="eprints.document_url" />
- <link rel="schema.DC" href="http://purl.org/DC/elements/1.0/" />
- <meta content="Vertical migration of the toxic dinoflagellate Gymnodinium
- catenatum under different concentrations of nutrients and
- humic substances in culture" name="DC.title" />
- <meta content="Doblin, Marina A." name="DC.creator" />
- <meta content="Thompson, Peter A." name="DC.creator" />
- <meta content="Revill, Andrew T." name="DC.creator" />
- <meta content="Bulter, Edward C.V." name="DC.creator" />
- <meta content="Blackburn, Susan I." name="DC.creator" />
- <meta content="Hallegraeff, Gustaaf M." name="DC.creator" />
- <meta content="270702 Marine and Estuarine Ecology (incl. Marine Ichthyology)" name="DC.subject" />
- <meta content="270700 Ecology and Evolution" name="DC.subject" />
- <meta content="260400 Oceanography" name="DC.subject" />
- <meta content="Vertical migration behaviour by the chainforming dinoflagellate Gymnodinium catenatum Graham was investigated using
- vertically-stratified laboratory columns. Under surface nutrient-deplete conditions, with nutrients added only at depth, 100% of
- cells underwent vertical migration (VM), starting downwards migration 3 h before the end of the light period and beginning
- upwards migration 3 h before the start of the light period. Cells in nutrient-replete columns showed no VM, but they were more
- dispersed in the upper layer during the dark compared to the light period. When surface layers (S) were nitrate-deplete (N) and
- enriched with humic substances (H) contained in Huon River water and bottom waters (B) were nutrient replete (R) (SHNBR), the
- pattern ofVMwas altered—50% of cells underwent migration and 50% remained at the pycnocline. In columns with nitrate-replete
- and humic-enriched surface layers (SHRBR), most cells underwent VM, while 30% remained at the surface. Cells in SHNBR
- columns showed increased N quotas and intra-cellular nitrate concentrations after 4 days, indicating nitrate uptake by G. catenatum
- in bottom layers. The concomitant increase in particulate organic nitrogen (PON) with the decrease in external nitrate
- concentrations in bottom layers provide convincing evidence that VM by G. catenatum facilitates nutrient retrieval at depth.
- However, addition of humic substances (a potential source of organic nitrogen) to surface layers did not ameliorate G. catenatum N
- depletion sufficiently to preclude the need for NO3 uptake at depth. Furthermore, there was no detectable pattern of increasing
- carbon (C) quota during the day (photosynthate accumulation) or increasing N quota during the night (nitrate assimilation). Toxic
- dinoflagellate G. catenatum blooms are commonly associated with nitrate depletion in surface waters in south-east Tasmanian
- waters (Australia). Therefore, vertical migration, facilitating N uptake at depth, could play an important role in this organism’s
- ecological strategy, enabling it to exploit environments where light and nutrients are vertically separated" name="DC.description" />
- <meta content="2006" name="DC.date" />
- <meta content="Article" name="DC.type" />
- <meta content="PeerReviewed" name="DC.type" />
- <meta content="application/pdf" name="DC.format" />
- <meta content="http://eprints.utas.edu.au/2498/1/Vertical_migration_of_the_tonic.pdf" name="DC.identifier" />
- <meta content="http://dx.doi.org/10.1016/j.hal.2006.02.002" name="DC.relation" />
- <meta content="Doblin, Marina A. and Thompson, Peter A. and Revill, Andrew T. and Bulter, Edward C.V. and Blackburn, Susan I. and Hallegraeff, Gustaaf M. (2006) Vertical migration of the toxic dinoflagellate Gymnodinium catenatum under different concentrations of nutrients and humic substances in culture. Harmful Algae, 5 (6). pp. 665-677. ISSN 1568-9883" name="DC.identifier" />
- <meta content="http://eprints.utas.edu.au/2498/" name="DC.relation" />
- <link rel="alternate" href="http://eprints.utas.edu.au/cgi/export/2498/BibTeX/epprod-eprint-2498.bib" title="BibTeX" type="text/plain" />
- <link rel="alternate" href="http://eprints.utas.edu.au/cgi/export/2498/ContextObject/epprod-eprint-2498.xml" title="OpenURL ContextObject" type="text/xml" />
- <link rel="alternate" href="http://eprints.utas.edu.au/cgi/export/2498/ContextObject::Dissertation/epprod-eprint-2498.xml" title="OpenURL Dissertation" type="text/xml" />
- <link rel="alternate" href="http://eprints.utas.edu.au/cgi/export/2498/ContextObject::Journal/epprod-eprint-2498.xml" title="OpenURL Journal" type="text/xml" />
- <link rel="alternate" href="http://eprints.utas.edu.au/cgi/export/2498/DC/epprod-eprint-2498.txt" title="Dublin Core" type="text/plain" />
- <link rel="alternate" href="http://eprints.utas.edu.au/cgi/export/2498/DIDL/epprod-eprint-2498.xml" title="DIDL" type="text/xml" />
- <link rel="alternate" href="http://eprints.utas.edu.au/cgi/export/2498/EndNote/epprod-eprint-2498.enw" title="EndNote" type="text/plain" />
- <link rel="alternate" href="http://eprints.utas.edu.au/cgi/export/2498/HTML/epprod-eprint-2498.html" title="HTML Citation" type="text/html; charset=utf-8" />
- <link rel="alternate" href="http://eprints.utas.edu.au/cgi/export/2498/METS/epprod-eprint-2498.xml" title="METS" type="text/xml" />
- <link rel="alternate" href="http://eprints.utas.edu.au/cgi/export/2498/MODS/epprod-eprint-2498.xml" title="MODS" type="text/xml" />
- <link rel="alternate" href="http://eprints.utas.edu.au/cgi/export/2498/RIS/epprod-eprint-2498.ris" title="Reference Manager" type="text/plain" />
- <link rel="alternate" href="http://eprints.utas.edu.au/cgi/export/2498/Refer/epprod-eprint-2498.refer" title="Refer" type="text/plain" />
- <link rel="alternate" href="http://eprints.utas.edu.au/cgi/export/2498/Simple/epprod-eprint-2498text" title="Simple Metadata" type="text/plain" />
- <link rel="alternate" href="http://eprints.utas.edu.au/cgi/export/2498/Text/epprod-eprint-2498.txt" title="ASCII Citation" type="text/plain; charset=utf-8" />
- <link rel="alternate" href="http://eprints.utas.edu.au/cgi/export/2498/XML/epprod-eprint-2498.xml" title="EP3 XML" type="text/xml" />
-
- </head>
- <body bgcolor="#ffffff" text="#000000" onLoad="loadRoutine(); MM_preloadImages('images/eprints/ePrints_banner_r5_c5_f2.gif','images/eprints/ePrints_banner_r5_c7_f2.gif','images/eprints/ePrints_banner_r5_c8_f2.gif','images/eprints/ePrints_banner_r5_c9_f2.gif','images/eprints/ePrints_banner_r5_c10_f2.gif','images/eprints/ePrints_banner_r5_c11_f2.gif','images/eprints/ePrints_banner_r6_c4_f2.gif')">
-
- <div class="ep_noprint"><noscript><style type="text/css">@import url(http://eprints.utas.edu.au/style/nojs.css);</style></noscript></div>
-
-
-
-
- <table width="795" border="0" cellspacing="0" cellpadding="0">
- <tr>
- <td><script language="JavaScript1.2">mmLoadMenus();</script>
- <table border="0" cellpadding="0" cellspacing="0" width="795">
- <!-- fwtable fwsrc="eprints_banner_final2.png" fwbase="ePrints_banner.gif" fwstyle="Dreamweaver" fwdocid = "1249563342" fwnested="0" -->
- <tr>
- <td><img src="/images/eprints/spacer.gif" width="32" height="1" border="0" alt="" /></td>
- <td><img src="/images/eprints/spacer.gif" width="104" height="1" border="0" alt="" /></td>
- <td><img src="/images/eprints/spacer.gif" width="44" height="1" border="0" alt="" /></td>
- <td><img src="/images/eprints/spacer.gif" width="105" height="1" border="0" alt="" /></td>
- <td><img src="/images/eprints/spacer.gif" width="41" height="1" border="0" alt="" /></td>
- <td><img src="/images/eprints/spacer.gif" width="16" height="1" border="0" alt="" /></td>
- <td><img src="/images/eprints/spacer.gif" width="68" height="1" border="0" alt="" /></td>
- <td><img src="/images/eprints/spacer.gif" width="68" height="1" border="0" alt="" /></td>
- <td><img src="/images/eprints/spacer.gif" width="68" height="1" border="0" alt="" /></td>
- <td><img src="/images/eprints/spacer.gif" width="82" height="1" border="0" alt="" /></td>
- <td><img src="/images/eprints/spacer.gif" width="69" height="1" border="0" alt="" /></td>
- <td><img src="/images/eprints/spacer.gif" width="98" height="1" border="0" alt="" /></td>
- <td><img src="/images/eprints/spacer.gif" width="1" height="1" border="0" alt="" /></td>
- </tr>
- <tr>
- <td colspan="12"><img name="ePrints_banner_r1_c1" src="/images/eprints/ePrints_banner_r1_c1.gif" width="795" height="10" border="0" alt="" /></td>
- <td><img src="/images/eprints/spacer.gif" width="1" height="10" border="0" alt="" /></td>
- </tr>
- <tr>
- <td rowspan="6"><img name="ePrints_banner_r2_c1" src="/images/eprints/ePrints_banner_r2_c1.gif" width="32" height="118" border="0" alt="" /></td>
- <td rowspan="5"><a href="http://www.utas.edu.au/"><img name="ePrints_banner_r2_c2" src="/images/eprints/ePrints_banner_r2_c2.gif" width="104" height="103" border="0" alt="" /></a></td>
- <td colspan="10"><img name="ePrints_banner_r2_c3" src="/images/eprints/ePrints_banner_r2_c3.gif" width="659" height="41" border="0" alt="" /></td>
- <td><img src="/images/eprints/spacer.gif" width="1" height="41" border="0" alt="" /></td>
- </tr>
- <tr>
- <td colspan="3"><a href="http://eprints.utas.edu.au/"><img name="ePrints_banner_r3_c3" src="/images/eprints/ePrints_banner_r3_c3.gif" width="190" height="31" border="0" alt="" /></a></td>
- <td rowspan="2" colspan="7"><img name="ePrints_banner_r3_c6" src="/images/eprints/ePrints_banner_r3_c6.gif" width="469" height="37" border="0" alt="" /></td>
- <td><img src="/images/eprints/spacer.gif" width="1" height="31" border="0" alt="" /></td>
- </tr>
- <tr>
- <td colspan="3"><img name="ePrints_banner_r4_c3" src="/images/eprints/ePrints_banner_r4_c3.gif" width="190" height="6" border="0" alt="" /></td>
- <td><img src="/images/eprints/spacer.gif" width="1" height="6" border="0" alt="" /></td>
- </tr>
- <tr>
- <td colspan="2"><img name="ePrints_banner_r5_c3" src="/images/eprints/ePrints_banner_r5_c3.gif" width="149" height="1" border="0" alt="" /></td>
- <td rowspan="2" colspan="2"><a href="/information.html" onMouseOut="MM_swapImgRestore();MM_startTimeout()" onMouseOver="MM_showMenu(window.mm_menu_0821132634_0,0,25,null,'ePrints_banner_r5_c5');MM_swapImage('ePrints_banner_r5_c5','','/images/eprints/ePrints_banner_r5_c5_f2.gif',1);"><img name="ePrints_banner_r5_c5" src="/images/eprints/ePrints_banner_r5_c5.gif" width="57" height="25" border="0" alt="About" /></a></td>
- <td rowspan="2"><a href="/view/" onMouseOut="MM_swapImgRestore();MM_startTimeout()" onMouseOver="MM_showMenu(window.mm_menu_0821133021_1,0,25,null,'ePrints_banner_r5_c7');MM_swapImage('ePrints_banner_r5_c7','','/images/eprints/ePrints_banner_r5_c7_f2.gif',1);"><img name="ePrints_banner_r5_c7" src="/images/eprints/ePrints_banner_r5_c7.gif" width="68" height="25" border="0" alt="Browse" /></a></td>
- <td rowspan="2"><a href="/perl/search/simple" onMouseOut="MM_swapImgRestore();MM_startTimeout()" onMouseOver="MM_showMenu(window.mm_menu_0821133201_2,0,25,null,'ePrints_banner_r5_c8');MM_swapImage('ePrints_banner_r5_c8','','/images/eprints/ePrints_banner_r5_c8_f2.gif',1);"><img name="ePrints_banner_r5_c8" src="/images/eprints/ePrints_banner_r5_c8.gif" width="68" height="25" border="0" alt="Search" /></a></td>
- <td rowspan="2"><a href="/perl/register" onMouseOut="MM_swapImgRestore();MM_startTimeout();" onMouseOver="MM_showMenu(window.mm_menu_1018171924_3,0,25,null,'ePrints_banner_r5_c9');MM_swapImage('ePrints_banner_r5_c9','','/images/eprints/ePrints_banner_r5_c9_f2.gif',1);"><img name="ePrints_banner_r5_c9" src="/images/eprints/ePrints_banner_r5_c9.gif" width="68" height="25" border="0" alt="register" /></a></td>
- <td rowspan="2"><a href="/perl/users/home" onMouseOut="MM_swapImgRestore();MM_startTimeout()" onMouseOver="MM_showMenu(window.mm_menu_0821133422_4,0,25,null,'ePrints_banner_r5_c10');MM_swapImage('ePrints_banner_r5_c10','','/images/eprints/ePrints_banner_r5_c10_f2.gif',1);"><img name="ePrints_banner_r5_c10" src="/images/eprints/ePrints_banner_r5_c10.gif" width="82" height="25" border="0" alt="user area" /></a></td>
- <td rowspan="2"><a href="/help/" onMouseOut="MM_swapImgRestore();MM_startTimeout()" onMouseOver="MM_showMenu(window.mm_menu_0821133514_5,0,25,null,'ePrints_banner_r5_c11');MM_swapImage('ePrints_banner_r5_c11','','/images/eprints/ePrints_banner_r5_c11_f2.gif',1);"><img name="ePrints_banner_r5_c11" src="/images/eprints/ePrints_banner_r5_c11.gif" width="69" height="25" border="0" alt="Help" /></a></td>
- <td rowspan="3" colspan="4"><img name="ePrints_banner_r5_c12" src="/images/eprints/ePrints_banner_r5_c12.gif" width="98" height="40" border="0" alt="" /></td>
- <td><img src="/images/eprints/spacer.gif" width="1" height="1" border="0" alt="" /></td>
- </tr>
- <tr>
- <td rowspan="2"><img name="ePrints_banner_r6_c3" src="/images/eprints/ePrints_banner_r6_c3.gif" width="44" height="39" border="0" alt="ePrints home" /></td>
- <td><a href="/" onMouseOut="MM_swapImgRestore()" onMouseOver="MM_swapImage('ePrints_banner_r6_c4','','/images/eprints/ePrints_banner_r6_c4_f2.gif',1);"><img name="ePrints_banner_r6_c4" src="/images/eprints/ePrints_banner_r6_c4.gif" width="105" height="24" border="0" alt="ePrints home" /></a></td>
- <td><img src="/images/eprints/spacer.gif" width="1" height="24" border="0" alt="" /></td>
- </tr>
- <tr>
- <td><img name="ePrints_banner_r7_c2" src="/images/eprints/ePrints_banner_r7_c2.gif" width="104" height="15" border="0" alt="" /></td>
- <td colspan="8"><img name="ePrints_banner_r7_c4" src="/images/eprints/ePrints_banner_r7_c4.gif" width="517" height="15" border="0" alt="" /></td>
- <td><img src="/images/eprints/spacer.gif" width="1" height="15" border="0" alt="" /></td>
- </tr>
- </table></td>
- </tr>
- <tr><td><table width="100%" style="font-size: 90%; border: solid 1px #ccc; padding: 3px"><tr>
- <td align="left"><a href="http://eprints.utas.edu.au/cgi/users/home">Login</a> | <a href="http://eprints.utas.edu.au/cgi/register">Create Account</a></td>
- <td align="right" style="white-space: nowrap">
- <form method="get" accept-charset="utf-8" action="http://eprints.utas.edu.au/cgi/search" style="display:inline">
- <input class="ep_tm_searchbarbox" size="20" type="text" name="q" />
- <input class="ep_tm_searchbarbutton" value="Search" type="submit" name="_action_search" />
- <input type="hidden" name="_order" value="bytitle" />
- <input type="hidden" name="basic_srchtype" value="ALL" />
- <input type="hidden" name="_satisfyall" value="ALL" />
- </form>
- </td>
- </tr></table></td></tr>
- <tr>
- <td class="toplinks"><!-- InstanceBeginEditable name="content" -->
-
-
- <div align="center">
-
- <table width="720" class="ep_tm_main"><tr><td align="left">
- <h1 class="ep_tm_pagetitle">Vertical migration of the toxic dinoflagellate Gymnodinium catenatum under different concentrations of nutrients and humic substances in culture</h1>
- <p style="margin-bottom: 1em" class="not_ep_block"><span class="person_name">Doblin, Marina A.</span> and <span class="person_name">Thompson, Peter A.</span> and <span class="person_name">Revill, Andrew T.</span> and <span class="person_name">Bulter, Edward C.V.</span> and <span class="person_name">Blackburn, Susan I.</span> and <span class="person_name">Hallegraeff, Gustaaf M.</span> (2006) <xhtml:em>Vertical migration of the toxic dinoflagellate Gymnodinium catenatum under different concentrations of nutrients and humic substances in culture.</xhtml:em> Harmful Algae, 5 (6). pp. 665-677. ISSN 1568-9883</p><p style="margin-bottom: 1em" class="not_ep_block"></p><table style="margin-bottom: 1em" class="not_ep_block"><tr><td valign="top" style="text-align:center"><a href="http://eprints.utas.edu.au/2498/1/Vertical_migration_of_the_tonic.pdf"><img alt="[img]" src="http://eprints.utas.edu.au/style/images/fileicons/application_pdf.png" class="ep_doc_icon" border="0" /></a></td><td valign="top"><a href="http://eprints.utas.edu.au/2498/1/Vertical_migration_of_the_tonic.pdf"><span class="ep_document_citation">PDF</span></a> - Full text restricted - Requires a PDF viewer<br />872Kb</td><td><form method="get" accept-charset="utf-8" action="http://eprints.utas.edu.au/cgi/request_doc"><input accept-charset="utf-8" value="3286" name="docid" type="hidden" /><div class=""><input value="Request a copy" name="_action_null" class="ep_form_action_button" onclick="return EPJS_button_pushed( '_action_null' )" type="submit" /> </div></form></td></tr></table><p style="margin-bottom: 1em" class="not_ep_block">Official URL: <a href="http://dx.doi.org/10.1016/j.hal.2006.02.002">http://dx.doi.org/10.1016/j.hal.2006.02.002</a></p><div class="not_ep_block"><h2>Abstract</h2><p style="padding-bottom: 16px; text-align: left; margin: 1em auto 0em auto">Vertical migration behaviour by the chainforming dinoflagellate Gymnodinium catenatum Graham was investigated using
- vertically-stratified laboratory columns. Under surface nutrient-deplete conditions, with nutrients added only at depth, 100% of
- cells underwent vertical migration (VM), starting downwards migration 3 h before the end of the light period and beginning
- upwards migration 3 h before the start of the light period. Cells in nutrient-replete columns showed no VM, but they were more
- dispersed in the upper layer during the dark compared to the light period. When surface layers (S) were nitrate-deplete (N) and
- enriched with humic substances (H) contained in Huon River water and bottom waters (B) were nutrient replete (R) (SHNBR), the
- pattern ofVMwas altered—50% of cells underwent migration and 50% remained at the pycnocline. In columns with nitrate-replete
- and humic-enriched surface layers (SHRBR), most cells underwent VM, while 30% remained at the surface. Cells in SHNBR
- columns showed increased N quotas and intra-cellular nitrate concentrations after 4 days, indicating nitrate uptake by G. catenatum
- in bottom layers. The concomitant increase in particulate organic nitrogen (PON) with the decrease in external nitrate
- concentrations in bottom layers provide convincing evidence that VM by G. catenatum facilitates nutrient retrieval at depth.
- However, addition of humic substances (a potential source of organic nitrogen) to surface layers did not ameliorate G. catenatum N
- depletion sufficiently to preclude the need for NO3 uptake at depth. Furthermore, there was no detectable pattern of increasing
- carbon (C) quota during the day (photosynthate accumulation) or increasing N quota during the night (nitrate assimilation). Toxic
- dinoflagellate G. catenatum blooms are commonly associated with nitrate depletion in surface waters in south-east Tasmanian
- waters (Australia). Therefore, vertical migration, facilitating N uptake at depth, could play an important role in this organism’s
- ecological strategy, enabling it to exploit environments where light and nutrients are vertically separated</p></div><table style="margin-bottom: 1em" cellpadding="3" class="not_ep_block" border="0"><tr><th valign="top" class="ep_row">Item Type:</th><td valign="top" class="ep_row">Article</td></tr><tr><th valign="top" class="ep_row">Additional Information:</th><td valign="top" class="ep_row">The definitive version is available at http://www.sciencedirect.com
- </td></tr><tr><th valign="top" class="ep_row">Keywords:</th><td valign="top" class="ep_row">CDOM; Gymnodinium catenatum; Humic substances; Nitrogen; N-stratified; Vertical migration</td></tr><tr><th valign="top" class="ep_row">Subjects:</th><td valign="top" class="ep_row"><a href="http://eprints.utas.edu.au/view/subjects/270702.html">270000 Biological Sciences > 270700 Ecology and Evolution > 270702 Marine and Estuarine Ecology (incl. Marine Ichthyology)</a><br /><a href="http://eprints.utas.edu.au/view/subjects/270700.html">270000 Biological Sciences > 270700 Ecology and Evolution</a><br /><a href="http://eprints.utas.edu.au/view/subjects/260400.html">260000 Earth Sciences > 260400 Oceanography</a></td></tr><tr><th valign="top" class="ep_row">ID Code:</th><td valign="top" class="ep_row">2498</td></tr><tr><th valign="top" class="ep_row">Deposited By:</th><td valign="top" class="ep_row"><span class="ep_name_citation"><span class="person_name">Scholarly Publications Librarian</span></span></td></tr><tr><th valign="top" class="ep_row">Deposited On:</th><td valign="top" class="ep_row">21 Nov 2007 09:24</td></tr><tr><th valign="top" class="ep_row">Last Modified:</th><td valign="top" class="ep_row">09 Jan 2008 02:30</td></tr><tr><th valign="top" class="ep_row">ePrint Statistics:</th><td valign="top" class="ep_row"><a target="ePrintStats" href="/es/index.php?action=show_detail_eprint;id=2498;">View statistics for this ePrint</a></td></tr></table><p align="right">Repository Staff Only: <a href="http://eprints.utas.edu.au/cgi/users/home?screen=EPrint::View&eprintid=2498">item control page</a></p>
- </td></tr></table>
- </div>
-
-
-
- <!-- InstanceEndEditable --></td>
- </tr>
- <tr>
- <td><!-- #BeginLibraryItem "/Library/footer_eprints.lbi" -->
- <table width="795" border="0" align="left" cellpadding="0" class="footer">
- <tr valign="top">
- <td colspan="2"><div align="center"><a href="http://www.utas.edu.au">UTAS home</a> | <a href="http://www.utas.edu.au/library/">Library home</a> | <a href="/">ePrints home</a> | <a href="/contact.html">contact</a> | <a href="/information.html">about</a> | <a href="/view/">browse</a> | <a href="/perl/search/simple">search</a> | <a href="/perl/register">register</a> | <a href="/perl/users/home">user area</a> | <a href="/help/">help</a></div><br /></td>
- </tr>
- <tr><td colspan="2"><p><img src="/images/eprints/footerline.gif" width="100%" height="4" /></p></td></tr>
- <tr valign="top">
- <td width="68%" class="footer">Authorised by the University Librarian<br />
- © University of Tasmania ABN 30 764 374 782<br />
- <a href="http://www.utas.edu.au/cricos/">CRICOS Provider Code 00586B</a> | <a href="http://www.utas.edu.au/copyright/copyright_disclaimers.html">Copyright & Disclaimers</a> | <a href="http://www.utas.edu.au/accessibility/index.html">Accessibility</a> | <a href="http://eprints.utas.edu.au/feedback/">Site Feedback</a> </td>
- <td width="32%"><div align="right">
- <p align="right" class="NoPrint"><a href="http://www.utas.edu.au/"><img src="http://www.utas.edu.au/shared/logos/unioftasstrip.gif" alt="University of Tasmania Home Page" width="260" height="16" border="0" align="right" /></a></p>
- <p align="right" class="NoPrint"><a href="http://www.utas.edu.au/"><br />
- </a></p>
- </div></td>
- </tr>
- <tr valign="top">
- <td><p> </p></td>
- <td><div align="right"><span class="NoPrint"><a href="http://www.eprints.org/software/"><img src="/images/eprintslogo.gif" alt="ePrints logo" width="77" height="29" border="0" align="bottom" /></a></span></div></td>
- </tr>
- </table>
- <!-- #EndLibraryItem -->
- <div align="center"></div></td>
- </tr>
- </table>
-
- </body>
- </html>