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    <title>UTas ePrints - Hybrid origin of Athrotaxis laxifolia (Taxodiaceae) confirmed by random amplified polymorphic DNA analysis</title>
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    <meta content="Isoda, Keiya" name="eprints.creators_name" />
<meta content="Brodribb, Tim J." name="eprints.creators_name" />
<meta content="Shiraishi, Susuma" name="eprints.creators_name" />
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<meta content="Timothy.Brodribb@utas.edu.au" name="eprints.creators_id" />
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<meta content="Hybrid origin of Athrotaxis laxifolia (Taxodiaceae) confirmed by
random amplified polymorphic DNA analysis" name="eprints.title" />
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<meta content="Random amplified polymorphic DNA (RAPD) and single-strand conformation polymorphism (SSCP)
analyses were employed for investigating genetic relationships of three Athrotaxis D.Don species. Twenty-nine
RAPD primers produced 103 polymorphic bands. Principal component analysis revealed the genomic differentiation
among three Athrotaxis species. Mean genetic distance (mean d) between A. selaginoides D.Don and A. cupressoides
D.Don was 0.89. Mean d values were reduced to 0.42/0.54 between A. laxifolia Hook. and A. selaginoides/A. cupressoides,
respectively. Intraspecific mean d of A. selaginoides and A. cupressoides were, respectively, 0.03 and 0.11. These
values indicated that A. laxifolia, which is regarded as a hybrid between A. selaginoides and A. cupressoides, is genetically
intermediate between A. selaginoides and A. cupressoides. This genetic characteristic and previously reported morphological
characteristics suggest the hybrid origins of A. laxifolia. The genomic composition of A. laxifolia was
estimated by the number of bands specific to A. selaginoides or A. cupressoides in order to determine the genomic contribution
of these two species to its proposed hybrid, A. laxifolia. All of the five individuals investigated herein possessed
genomes derived almost evenly from A. selaginoides and A. cupressoides. Furthermore, the pollen donor of
A. laxifolia was determined by SSCP analysis of the atpAgene on chloroplast DNA. Because all of the five A. laxifolia
possessed the A. selaginoides-type chloroplast genome, A. laxifolia would be a hybrid of A. selaginoides as a paternal
parent and A. cupressoides as a maternal parent." name="eprints.abstract" />
<meta content="2000" name="eprints.date" />
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<meta content="Australian Journal of Botany" name="eprints.publication" />
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Clifford HT, Constantine J (1980) ‘Ferns, fern allies and conifers of
Australia. A laboratory manual.’ (University of Queensland Press:
Brisbane)
Cullen PJ, Kirkpatrick JB (1988) The ecology of Athrotaxis D.Don
(Taxodiaceae). II. The distributions and ecological differentiation of
A. cupressoides and A. selaginoides. Australian Journal of Botany
36, 561–573.
Curtis WM (1956) ‘The student’s flora of Tasmania. Part 1.’ (LG Shea,
Government Printer: Tasmania)
Curtis WM (1967) ‘The endemic flora of Tasmania.’ (Ariel Press:
London)
Florin R (1963) The distribution of conifer and taxad genera in time and
space. Acta Horti Bergiani 20, 121–312.
Gulline HF (1952) The cytology of Athrotaxis. Papers and Proceedings
of the Royal Society of Tasmania 86, 131–136.
Hongyo T, Buzard GS, Calvert RJ, Weghorst CM (1993) ‘Cold SSCP’:
a simple, rapid and non-radioactive method for optimized singlestrand
conformation polymorphism analyses. Nucleic Acids Research
21, 3637–3642.
Murray MG, Thompson WF (1980) Rapid isolation of high molecular
weight plant DNA. Nucleic Acids Research 8, 4321–4325.
Narasaki K, Watanabe A, Tomita K, Sasaki Y, Shiraishi S (1996) DNA
analysis of the interspecific hybrid clones and the horticultural varieties
of Chamaecyparis obtusa and C. pisifera. Journal of the
Japanese Forestry Society 78, 157–161.
Neale DB, Sederoff RR (1989) Paternal inheritance of chloroplast DNA
and maternal inheritance of mitochondrial DNA in loblolly pine.
Theoretical and Applied Genetics 77, 212–216.
Neale DB, Wheeler NC, Allard RW (1986) Paternal inheritance of
chloroplast DNA in Douglas-fir. Canadian Journal of Forest
Research 16, 1152–1154.
Neale DB, Marshall KA, Sederoff RR (1989) Chloroplast and mitochondrial
DNA are paternally inherited in Sequoia sempervirens
D.Don Endl. Proceedings of the National Academy of Sciences of the
United States of America 86, 9347–9349.
Neale DB, Marshall KA, Harry DE (1991) Inheritance of chloroplast
and mitochondrial DNA in incense-ceder (Calocedrus decurrens).
Canadian Journal of Forest Research 21, 717–720.
Orita M, Iwahana H, Kanazawa H, Hayashi K, Sekiya T(1989a) Detection
of polymorphisms of human DNA by gel electrophoresis as singlestrand
conformation polymorphisms. Proceedings of the National
Academy of Sciences of the United States of America 86, 2766–2770.
Orita M, Susuki Y, Sekiya T, Hayashi K (1989b) Rapid and sensitive
detection of point mutations and DNA polymorphisms using the
polymerase chain reaction. Genomics 5, 874–879.
Sambrook J, Fritsch EF, Maniatis T (1989) ‘Molecular cloning: a laboratory
manual.’ (2nd Edn) (Cold Spring Harbor Laboratory Press:
Cold Spring Harbor, NY)
Shiraishi S, Watanabe A (1995) Identification of chloroplast genome
between Pinus densiflora Sieb. et Zucc. and P. thunbergii Parl.
based on the polymorphism in rbcL gene. Journal of the Japanese
Forestry Society 77, 429–436.
Sokal PR, Michener CD (1958) Astatistical method for evaluating systematic
relationships. The University of Kansas Science Bulletin 38,
1409–1438.
Stine M, Sears BB, Keathley DE (1989) Inheritance of plastids in interspecific
hybrids of blue spruce and white spruce. Theoretical and
Applied Genetics 78, 768–774.
Szmidt AE, Alden T, Hallgren J-E (1987) Paternal inheritance of chloroplast
DNA in Larix. Plant Molecular Biology 9, 59–64.
Szmidt AE, El-Kassaby YA, Sigurgeirson A, Alden T, Lindgren D,
Hallgren J-E (1988) Classifying seedlots of Picea sitchensis and
P. glauca in zones of introgression, using restriction analysis of
chloroplast DNA. Theoretical and Applied Genetics 76, 841–845.
Tsumura Y, Yoshimura K, Tomaru N, Ohba K (1995) Molecular phylogeny
of conifers using RFLP analysis of PCR-amplified specific
chloroplast genes. Theoretical and Applied Genetics 91, 1222–1236.
Vendramin GG, Ziegenhagen B (1997) Characterisation and inheritance
of polymorphic plastid microsatellites in Abies. Genome 40, 857–864.
Wagner DB, Furnier GR, Saghai-Maroof MA, Williams SM,
Dancik BP, Allard RW (1987) Chloroplast DNA polymorphisms in
lodgepole and jack pines and their hybrids. Proceedings of the
National Academy of Sciences of the United States of America 84,
2097–2100.
Wagner DB, Govindaraju DR, Yeatman CW, Pitel JA (1989) Paternal
chloroplast DNA inheritance in a diallel cross of jack pine (Pinus
banksiana Lamb.). Journal of Heredity 80, 483–485.
Williams JGK, Kubelik AR, Livak KJ, Rafalski JA, Tingey SV (1990)
DNA polymorphisms amplified by arbitrary primers are useful as
genetic markers. Nucleic Acids Research 18, 6531–6535." name="eprints.referencetext" />
<meta content="Isoda, Keiya and Brodribb, Tim J. and Shiraishi, Susuma (2000) Hybrid origin of Athrotaxis laxifolia (Taxodiaceae) confirmed by random amplified polymorphic DNA analysis. Australian Journal of Botany, 48 (6). pp. 753-758. ISSN 0067-1924" name="eprints.citation" />
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random amplified polymorphic DNA analysis" name="DC.title" />
<meta content="Isoda, Keiya" name="DC.creator" />
<meta content="Brodribb, Tim J." name="DC.creator" />
<meta content="Shiraishi, Susuma" name="DC.creator" />
<meta content="Random amplified polymorphic DNA (RAPD) and single-strand conformation polymorphism (SSCP)
analyses were employed for investigating genetic relationships of three Athrotaxis D.Don species. Twenty-nine
RAPD primers produced 103 polymorphic bands. Principal component analysis revealed the genomic differentiation
among three Athrotaxis species. Mean genetic distance (mean d) between A. selaginoides D.Don and A. cupressoides
D.Don was 0.89. Mean d values were reduced to 0.42/0.54 between A. laxifolia Hook. and A. selaginoides/A. cupressoides,
respectively. Intraspecific mean d of A. selaginoides and A. cupressoides were, respectively, 0.03 and 0.11. These
values indicated that A. laxifolia, which is regarded as a hybrid between A. selaginoides and A. cupressoides, is genetically
intermediate between A. selaginoides and A. cupressoides. This genetic characteristic and previously reported morphological
characteristics suggest the hybrid origins of A. laxifolia. The genomic composition of A. laxifolia was
estimated by the number of bands specific to A. selaginoides or A. cupressoides in order to determine the genomic contribution
of these two species to its proposed hybrid, A. laxifolia. All of the five individuals investigated herein possessed
genomes derived almost evenly from A. selaginoides and A. cupressoides. Furthermore, the pollen donor of
A. laxifolia was determined by SSCP analysis of the atpAgene on chloroplast DNA. Because all of the five A. laxifolia
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    <h1 class="ep_tm_pagetitle">Hybrid origin of Athrotaxis laxifolia (Taxodiaceae) confirmed by random amplified polymorphic DNA analysis</h1>
    <p style="margin-bottom: 1em" class="not_ep_block"><span class="person_name">Isoda, Keiya</span> and <span class="person_name">Brodribb, Tim J.</span> and <span class="person_name">Shiraishi, Susuma</span> (2000) <xhtml:em>Hybrid origin of Athrotaxis laxifolia (Taxodiaceae) confirmed by random amplified polymorphic DNA analysis.</xhtml:em> Australian Journal of Botany, 48 (6). pp. 753-758. ISSN 0067-1924</p><p style="margin-bottom: 1em" class="not_ep_block"></p><table style="margin-bottom: 1em" class="not_ep_block"><tr><td valign="top" style="text-align:center"><a href="http://eprints.utas.edu.au/2633/1/Athero_lax.pdf"><img alt="[img]" src="http://eprints.utas.edu.au/style/images/fileicons/application_pdf.png" class="ep_doc_icon" border="0" /></a></td><td valign="top"><a href="http://eprints.utas.edu.au/2633/1/Athero_lax.pdf"><span class="ep_document_citation">PDF</span></a> - Full text restricted - Requires a PDF viewer<br />477Kb</td><td><form method="get" accept-charset="utf-8" action="http://eprints.utas.edu.au/cgi/request_doc"><input accept-charset="utf-8" value="3447" name="docid" type="hidden" /><div class=""><input value="Request a copy" name="_action_null" class="ep_form_action_button" onclick="return EPJS_button_pushed( '_action_null' )" type="submit" /> </div></form></td></tr></table><p style="margin-bottom: 1em" class="not_ep_block">Official URL: <a href="http://dx.doi.org/10.1071/BT99032">http://dx.doi.org/10.1071/BT99032</a></p><div class="not_ep_block"><h2>Abstract</h2><p style="padding-bottom: 16px; text-align: left; margin: 1em auto 0em auto">Random amplified polymorphic DNA (RAPD) and single-strand conformation polymorphism (SSCP)&#13;
analyses were employed for investigating genetic relationships of three Athrotaxis D.Don species. Twenty-nine&#13;
RAPD primers produced 103 polymorphic bands. Principal component analysis revealed the genomic differentiation&#13;
among three Athrotaxis species. Mean genetic distance (mean d) between A. selaginoides D.Don and A. cupressoides&#13;
D.Don was 0.89. Mean d values were reduced to 0.42/0.54 between A. laxifolia Hook. and A. selaginoides/A. cupressoides,&#13;
respectively. Intraspecific mean d of A. selaginoides and A. cupressoides were, respectively, 0.03 and 0.11. These&#13;
values indicated that A. laxifolia, which is regarded as a hybrid between A. selaginoides and A. cupressoides, is genetically&#13;
intermediate between A. selaginoides and A. cupressoides. This genetic characteristic and previously reported morphological&#13;
characteristics suggest the hybrid origins of A. laxifolia. The genomic composition of A. laxifolia was&#13;
estimated by the number of bands specific to A. selaginoides or A. cupressoides in order to determine the genomic contribution&#13;
of these two species to its proposed hybrid, A. laxifolia. All of the five individuals investigated herein possessed&#13;
genomes derived almost evenly from A. selaginoides and A. cupressoides. Furthermore, the pollen donor of&#13;
A. laxifolia was determined by SSCP analysis of the atpAgene on chloroplast DNA. Because all of the five A. laxifolia&#13;
possessed the A. selaginoides-type chloroplast genome, A. laxifolia would be a hybrid of A. selaginoides as a paternal&#13;
parent and A. cupressoides as a maternal parent.</p></div><table style="margin-bottom: 1em" cellpadding="3" class="not_ep_block" border="0"><tr><th valign="top" class="ep_row">Item Type:</th><td valign="top" class="ep_row">Article</td></tr><tr><th valign="top" class="ep_row">Subjects:</th><td valign="top" class="ep_row">UNSPECIFIED</td></tr><tr><th valign="top" class="ep_row">ID Code:</th><td valign="top" class="ep_row">2633</td></tr><tr><th valign="top" class="ep_row">Deposited By:</th><td valign="top" class="ep_row"><span class="ep_name_citation"><span class="person_name">Scholarly Publications Librarian</span></span></td></tr><tr><th valign="top" class="ep_row">Deposited On:</th><td valign="top" class="ep_row">05 Dec 2007 12:19</td></tr><tr><th valign="top" class="ep_row">Last Modified:</th><td valign="top" class="ep_row">09 Jan 2008 02:30</td></tr><tr><th valign="top" class="ep_row">ePrint Statistics:</th><td valign="top" class="ep_row"><a target="ePrintStats" href="/es/index.php?action=show_detail_eprint;id=2633;">View statistics for this ePrint</a></td></tr></table><p align="right">Repository Staff Only: <a href="http://eprints.utas.edu.au/cgi/users/home?screen=EPrint::View&amp;eprintid=2633">item control page</a></p>
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