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<title>UTas ePrints - Hybrid origin of Athrotaxis laxifolia (Taxodiaceae) confirmed by random amplified polymorphic DNA analysis</title>
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<meta content="Isoda, Keiya" name="eprints.creators_name" />
<meta content="Brodribb, Tim J." name="eprints.creators_name" />
<meta content="Shiraishi, Susuma" name="eprints.creators_name" />
<meta content="" name="eprints.creators_id" />
<meta content="Timothy.Brodribb@utas.edu.au" name="eprints.creators_id" />
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<meta content="article" name="eprints.type" />
<meta content="2007-12-05 01:19:11" name="eprints.datestamp" />
<meta content="2008-01-08 15:30:00" name="eprints.lastmod" />
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<meta content="Hybrid origin of Athrotaxis laxifolia (Taxodiaceae) confirmed by
random amplified polymorphic DNA analysis" name="eprints.title" />
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<meta content="Random amplified polymorphic DNA (RAPD) and single-strand conformation polymorphism (SSCP)
analyses were employed for investigating genetic relationships of three Athrotaxis D.Don species. Twenty-nine
RAPD primers produced 103 polymorphic bands. Principal component analysis revealed the genomic differentiation
among three Athrotaxis species. Mean genetic distance (mean d) between A. selaginoides D.Don and A. cupressoides
D.Don was 0.89. Mean d values were reduced to 0.42/0.54 between A. laxifolia Hook. and A. selaginoides/A. cupressoides,
respectively. Intraspecific mean d of A. selaginoides and A. cupressoides were, respectively, 0.03 and 0.11. These
values indicated that A. laxifolia, which is regarded as a hybrid between A. selaginoides and A. cupressoides, is genetically
intermediate between A. selaginoides and A. cupressoides. This genetic characteristic and previously reported morphological
characteristics suggest the hybrid origins of A. laxifolia. The genomic composition of A. laxifolia was
estimated by the number of bands specific to A. selaginoides or A. cupressoides in order to determine the genomic contribution
of these two species to its proposed hybrid, A. laxifolia. All of the five individuals investigated herein possessed
genomes derived almost evenly from A. selaginoides and A. cupressoides. Furthermore, the pollen donor of
A. laxifolia was determined by SSCP analysis of the atpAgene on chloroplast DNA. Because all of the five A. laxifolia
possessed the A. selaginoides-type chloroplast genome, A. laxifolia would be a hybrid of A. selaginoides as a paternal
parent and A. cupressoides as a maternal parent." name="eprints.abstract" />
<meta content="2000" name="eprints.date" />
<meta content="published" name="eprints.date_type" />
<meta content="Australian Journal of Botany" name="eprints.publication" />
<meta content="48" name="eprints.volume" />
<meta content="6" name="eprints.number" />
<meta content="753-758" name="eprints.pagerange" />
<meta content="10.1071/BT99032" name="eprints.id_number" />
<meta content="TRUE" name="eprints.refereed" />
<meta content="0067-1924" name="eprints.issn" />
<meta content="http://dx.doi.org/10.1071/BT99032" name="eprints.official_url" />
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genetic markers. Nucleic Acids Research 18, 6531–6535." name="eprints.referencetext" />
<meta content="Isoda, Keiya and Brodribb, Tim J. and Shiraishi, Susuma (2000) Hybrid origin of Athrotaxis laxifolia (Taxodiaceae) confirmed by random amplified polymorphic DNA analysis. Australian Journal of Botany, 48 (6). pp. 753-758. ISSN 0067-1924" name="eprints.citation" />
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random amplified polymorphic DNA analysis" name="DC.title" />
<meta content="Isoda, Keiya" name="DC.creator" />
<meta content="Brodribb, Tim J." name="DC.creator" />
<meta content="Shiraishi, Susuma" name="DC.creator" />
<meta content="Random amplified polymorphic DNA (RAPD) and single-strand conformation polymorphism (SSCP)
analyses were employed for investigating genetic relationships of three Athrotaxis D.Don species. Twenty-nine
RAPD primers produced 103 polymorphic bands. Principal component analysis revealed the genomic differentiation
among three Athrotaxis species. Mean genetic distance (mean d) between A. selaginoides D.Don and A. cupressoides
D.Don was 0.89. Mean d values were reduced to 0.42/0.54 between A. laxifolia Hook. and A. selaginoides/A. cupressoides,
respectively. Intraspecific mean d of A. selaginoides and A. cupressoides were, respectively, 0.03 and 0.11. These
values indicated that A. laxifolia, which is regarded as a hybrid between A. selaginoides and A. cupressoides, is genetically
intermediate between A. selaginoides and A. cupressoides. This genetic characteristic and previously reported morphological
characteristics suggest the hybrid origins of A. laxifolia. The genomic composition of A. laxifolia was
estimated by the number of bands specific to A. selaginoides or A. cupressoides in order to determine the genomic contribution
of these two species to its proposed hybrid, A. laxifolia. All of the five individuals investigated herein possessed
genomes derived almost evenly from A. selaginoides and A. cupressoides. Furthermore, the pollen donor of
A. laxifolia was determined by SSCP analysis of the atpAgene on chloroplast DNA. Because all of the five A. laxifolia
possessed the A. selaginoides-type chloroplast genome, A. laxifolia would be a hybrid of A. selaginoides as a paternal
parent and A. cupressoides as a maternal parent." name="DC.description" />
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<h1 class="ep_tm_pagetitle">Hybrid origin of Athrotaxis laxifolia (Taxodiaceae) confirmed by random amplified polymorphic DNA analysis</h1>
<p style="margin-bottom: 1em" class="not_ep_block"><span class="person_name">Isoda, Keiya</span> and <span class="person_name">Brodribb, Tim J.</span> and <span class="person_name">Shiraishi, Susuma</span> (2000) <xhtml:em>Hybrid origin of Athrotaxis laxifolia (Taxodiaceae) confirmed by random amplified polymorphic DNA analysis.</xhtml:em> Australian Journal of Botany, 48 (6). pp. 753-758. ISSN 0067-1924</p><p style="margin-bottom: 1em" class="not_ep_block"></p><table style="margin-bottom: 1em" class="not_ep_block"><tr><td valign="top" style="text-align:center"><a href="http://eprints.utas.edu.au/2633/1/Athero_lax.pdf"><img alt="[img]" src="http://eprints.utas.edu.au/style/images/fileicons/application_pdf.png" class="ep_doc_icon" border="0" /></a></td><td valign="top"><a href="http://eprints.utas.edu.au/2633/1/Athero_lax.pdf"><span class="ep_document_citation">PDF</span></a> - Full text restricted - Requires a PDF viewer<br />477Kb</td><td><form method="get" accept-charset="utf-8" action="http://eprints.utas.edu.au/cgi/request_doc"><input accept-charset="utf-8" value="3447" name="docid" type="hidden" /><div class=""><input value="Request a copy" name="_action_null" class="ep_form_action_button" onclick="return EPJS_button_pushed( '_action_null' )" type="submit" /> </div></form></td></tr></table><p style="margin-bottom: 1em" class="not_ep_block">Official URL: <a href="http://dx.doi.org/10.1071/BT99032">http://dx.doi.org/10.1071/BT99032</a></p><div class="not_ep_block"><h2>Abstract</h2><p style="padding-bottom: 16px; text-align: left; margin: 1em auto 0em auto">Random amplified polymorphic DNA (RAPD) and single-strand conformation polymorphism (SSCP)
analyses were employed for investigating genetic relationships of three Athrotaxis D.Don species. Twenty-nine
RAPD primers produced 103 polymorphic bands. Principal component analysis revealed the genomic differentiation
among three Athrotaxis species. Mean genetic distance (mean d) between A. selaginoides D.Don and A. cupressoides
D.Don was 0.89. Mean d values were reduced to 0.42/0.54 between A. laxifolia Hook. and A. selaginoides/A. cupressoides,
respectively. Intraspecific mean d of A. selaginoides and A. cupressoides were, respectively, 0.03 and 0.11. These
values indicated that A. laxifolia, which is regarded as a hybrid between A. selaginoides and A. cupressoides, is genetically
intermediate between A. selaginoides and A. cupressoides. This genetic characteristic and previously reported morphological
characteristics suggest the hybrid origins of A. laxifolia. The genomic composition of A. laxifolia was
estimated by the number of bands specific to A. selaginoides or A. cupressoides in order to determine the genomic contribution
of these two species to its proposed hybrid, A. laxifolia. All of the five individuals investigated herein possessed
genomes derived almost evenly from A. selaginoides and A. cupressoides. Furthermore, the pollen donor of
A. laxifolia was determined by SSCP analysis of the atpAgene on chloroplast DNA. Because all of the five A. laxifolia
possessed the A. selaginoides-type chloroplast genome, A. laxifolia would be a hybrid of A. selaginoides as a paternal
parent and A. cupressoides as a maternal parent.</p></div><table style="margin-bottom: 1em" cellpadding="3" class="not_ep_block" border="0"><tr><th valign="top" class="ep_row">Item Type:</th><td valign="top" class="ep_row">Article</td></tr><tr><th valign="top" class="ep_row">Subjects:</th><td valign="top" class="ep_row">UNSPECIFIED</td></tr><tr><th valign="top" class="ep_row">ID Code:</th><td valign="top" class="ep_row">2633</td></tr><tr><th valign="top" class="ep_row">Deposited By:</th><td valign="top" class="ep_row"><span class="ep_name_citation"><span class="person_name">Scholarly Publications Librarian</span></span></td></tr><tr><th valign="top" class="ep_row">Deposited On:</th><td valign="top" class="ep_row">05 Dec 2007 12:19</td></tr><tr><th valign="top" class="ep_row">Last Modified:</th><td valign="top" class="ep_row">09 Jan 2008 02:30</td></tr><tr><th valign="top" class="ep_row">ePrint Statistics:</th><td valign="top" class="ep_row"><a target="ePrintStats" href="/es/index.php?action=show_detail_eprint;id=2633;">View statistics for this ePrint</a></td></tr></table><p align="right">Repository Staff Only: <a href="http://eprints.utas.edu.au/cgi/users/home?screen=EPrint::View&eprintid=2633">item control page</a></p>
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