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  5. <title>UTas ePrints - Hybrid origin of Athrotaxis laxifolia (Taxodiaceae) confirmed by random amplified polymorphic DNA analysis</title>
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  13. <meta content="Isoda, Keiya" name="eprints.creators_name" />
  14. <meta content="Brodribb, Tim J." name="eprints.creators_name" />
  15. <meta content="Shiraishi, Susuma" name="eprints.creators_name" />
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  17. <meta content="Timothy.Brodribb@utas.edu.au" name="eprints.creators_id" />
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  20. <meta content="2007-12-05 01:19:11" name="eprints.datestamp" />
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  23. <meta content="Hybrid origin of Athrotaxis laxifolia (Taxodiaceae) confirmed by
  24. random amplified polymorphic DNA analysis" name="eprints.title" />
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  27. <meta content="Random amplified polymorphic DNA (RAPD) and single-strand conformation polymorphism (SSCP)
  28. analyses were employed for investigating genetic relationships of three Athrotaxis D.Don species. Twenty-nine
  29. RAPD primers produced 103 polymorphic bands. Principal component analysis revealed the genomic differentiation
  30. among three Athrotaxis species. Mean genetic distance (mean d) between A. selaginoides D.Don and A. cupressoides
  31. D.Don was 0.89. Mean d values were reduced to 0.42/0.54 between A. laxifolia Hook. and A. selaginoides/A. cupressoides,
  32. respectively. Intraspecific mean d of A. selaginoides and A. cupressoides were, respectively, 0.03 and 0.11. These
  33. values indicated that A. laxifolia, which is regarded as a hybrid between A. selaginoides and A. cupressoides, is genetically
  34. intermediate between A. selaginoides and A. cupressoides. This genetic characteristic and previously reported morphological
  35. characteristics suggest the hybrid origins of A. laxifolia. The genomic composition of A. laxifolia was
  36. estimated by the number of bands specific to A. selaginoides or A. cupressoides in order to determine the genomic contribution
  37. of these two species to its proposed hybrid, A. laxifolia. All of the five individuals investigated herein possessed
  38. genomes derived almost evenly from A. selaginoides and A. cupressoides. Furthermore, the pollen donor of
  39. A. laxifolia was determined by SSCP analysis of the atpAgene on chloroplast DNA. Because all of the five A. laxifolia
  40. possessed the A. selaginoides-type chloroplast genome, A. laxifolia would be a hybrid of A. selaginoides as a paternal
  41. parent and A. cupressoides as a maternal parent." name="eprints.abstract" />
  42. <meta content="2000" name="eprints.date" />
  43. <meta content="published" name="eprints.date_type" />
  44. <meta content="Australian Journal of Botany" name="eprints.publication" />
  45. <meta content="48" name="eprints.volume" />
  46. <meta content="6" name="eprints.number" />
  47. <meta content="753-758" name="eprints.pagerange" />
  48. <meta content="10.1071/BT99032" name="eprints.id_number" />
  49. <meta content="TRUE" name="eprints.refereed" />
  50. <meta content="0067-1924" name="eprints.issn" />
  51. <meta content="http://dx.doi.org/10.1071/BT99032" name="eprints.official_url" />
  52. <meta content="
  53. Clifford HT, Constantine J (1980) ‘Ferns, fern allies and conifers of
  54. Australia. A laboratory manual.’ (University of Queensland Press:
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  56. Cullen PJ, Kirkpatrick JB (1988) The ecology of Athrotaxis D.Don
  57. (Taxodiaceae). II. The distributions and ecological differentiation of
  58. A. cupressoides and A. selaginoides. Australian Journal of Botany
  59. 36, 561–573.
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  68. Hongyo T, Buzard GS, Calvert RJ, Weghorst CM (1993) ‘Cold SSCP’:
  69. a simple, rapid and non-radioactive method for optimized singlestrand
  70. conformation polymorphism analyses. Nucleic Acids Research
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  78. Neale DB, Sederoff RR (1989) Paternal inheritance of chloroplast DNA
  79. and maternal inheritance of mitochondrial DNA in loblolly pine.
  80. Theoretical and Applied Genetics 77, 212–216.
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  82. chloroplast DNA in Douglas-fir. Canadian Journal of Forest
  83. Research 16, 1152–1154.
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  85. DNA are paternally inherited in Sequoia sempervirens
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  87. United States of America 86, 9347–9349.
  88. Neale DB, Marshall KA, Harry DE (1991) Inheritance of chloroplast
  89. and mitochondrial DNA in incense-ceder (Calocedrus decurrens).
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  91. Orita M, Iwahana H, Kanazawa H, Hayashi K, Sekiya T(1989a) Detection
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  99. manual.’ (2nd Edn) (Cold Spring Harbor Laboratory Press:
  100. Cold Spring Harbor, NY)
  101. Shiraishi S, Watanabe A (1995) Identification of chloroplast genome
  102. between Pinus densiflora Sieb. et Zucc. and P. thunbergii Parl.
  103. based on the polymorphism in rbcL gene. Journal of the Japanese
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  109. hybrids of blue spruce and white spruce. Theoretical and
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  112. DNA in Larix. Plant Molecular Biology 9, 59–64.
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  114. Hallgren J-E (1988) Classifying seedlots of Picea sitchensis and
  115. P. glauca in zones of introgression, using restriction analysis of
  116. chloroplast DNA. Theoretical and Applied Genetics 76, 841–845.
  117. Tsumura Y, Yoshimura K, Tomaru N, Ohba K (1995) Molecular phylogeny
  118. of conifers using RFLP analysis of PCR-amplified specific
  119. chloroplast genes. Theoretical and Applied Genetics 91, 1222–1236.
  120. Vendramin GG, Ziegenhagen B (1997) Characterisation and inheritance
  121. of polymorphic plastid microsatellites in Abies. Genome 40, 857–864.
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  123. Dancik BP, Allard RW (1987) Chloroplast DNA polymorphisms in
  124. lodgepole and jack pines and their hybrids. Proceedings of the
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  127. Wagner DB, Govindaraju DR, Yeatman CW, Pitel JA (1989) Paternal
  128. chloroplast DNA inheritance in a diallel cross of jack pine (Pinus
  129. banksiana Lamb.). Journal of Heredity 80, 483–485.
  130. Williams JGK, Kubelik AR, Livak KJ, Rafalski JA, Tingey SV (1990)
  131. DNA polymorphisms amplified by arbitrary primers are useful as
  132. genetic markers. Nucleic Acids Research 18, 6531–6535." name="eprints.referencetext" />
  133. <meta content="Isoda, Keiya and Brodribb, Tim J. and Shiraishi, Susuma (2000) Hybrid origin of Athrotaxis laxifolia (Taxodiaceae) confirmed by random amplified polymorphic DNA analysis. Australian Journal of Botany, 48 (6). pp. 753-758. ISSN 0067-1924" name="eprints.citation" />
  134. <meta content="http://eprints.utas.edu.au/2633/1/Athero_lax.pdf" name="eprints.document_url" />
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  136. <meta content="Hybrid origin of Athrotaxis laxifolia (Taxodiaceae) confirmed by
  137. random amplified polymorphic DNA analysis" name="DC.title" />
  138. <meta content="Isoda, Keiya" name="DC.creator" />
  139. <meta content="Brodribb, Tim J." name="DC.creator" />
  140. <meta content="Shiraishi, Susuma" name="DC.creator" />
  141. <meta content="Random amplified polymorphic DNA (RAPD) and single-strand conformation polymorphism (SSCP)
  142. analyses were employed for investigating genetic relationships of three Athrotaxis D.Don species. Twenty-nine
  143. RAPD primers produced 103 polymorphic bands. Principal component analysis revealed the genomic differentiation
  144. among three Athrotaxis species. Mean genetic distance (mean d) between A. selaginoides D.Don and A. cupressoides
  145. D.Don was 0.89. Mean d values were reduced to 0.42/0.54 between A. laxifolia Hook. and A. selaginoides/A. cupressoides,
  146. respectively. Intraspecific mean d of A. selaginoides and A. cupressoides were, respectively, 0.03 and 0.11. These
  147. values indicated that A. laxifolia, which is regarded as a hybrid between A. selaginoides and A. cupressoides, is genetically
  148. intermediate between A. selaginoides and A. cupressoides. This genetic characteristic and previously reported morphological
  149. characteristics suggest the hybrid origins of A. laxifolia. The genomic composition of A. laxifolia was
  150. estimated by the number of bands specific to A. selaginoides or A. cupressoides in order to determine the genomic contribution
  151. of these two species to its proposed hybrid, A. laxifolia. All of the five individuals investigated herein possessed
  152. genomes derived almost evenly from A. selaginoides and A. cupressoides. Furthermore, the pollen donor of
  153. A. laxifolia was determined by SSCP analysis of the atpAgene on chloroplast DNA. Because all of the five A. laxifolia
  154. possessed the A. selaginoides-type chloroplast genome, A. laxifolia would be a hybrid of A. selaginoides as a paternal
  155. parent and A. cupressoides as a maternal parent." name="DC.description" />
  156. <meta content="2000" name="DC.date" />
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  162. <meta content="Isoda, Keiya and Brodribb, Tim J. and Shiraishi, Susuma (2000) Hybrid origin of Athrotaxis laxifolia (Taxodiaceae) confirmed by random amplified polymorphic DNA analysis. Australian Journal of Botany, 48 (6). pp. 753-758. ISSN 0067-1924" name="DC.identifier" />
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  267. <h1 class="ep_tm_pagetitle">Hybrid origin of Athrotaxis laxifolia (Taxodiaceae) confirmed by random amplified polymorphic DNA analysis</h1>
  268. <p style="margin-bottom: 1em" class="not_ep_block"><span class="person_name">Isoda, Keiya</span> and <span class="person_name">Brodribb, Tim J.</span> and <span class="person_name">Shiraishi, Susuma</span> (2000) <xhtml:em>Hybrid origin of Athrotaxis laxifolia (Taxodiaceae) confirmed by random amplified polymorphic DNA analysis.</xhtml:em> Australian Journal of Botany, 48 (6). pp. 753-758. ISSN 0067-1924</p><p style="margin-bottom: 1em" class="not_ep_block"></p><table style="margin-bottom: 1em" class="not_ep_block"><tr><td valign="top" style="text-align:center"><a href="http://eprints.utas.edu.au/2633/1/Athero_lax.pdf"><img alt="[img]" src="http://eprints.utas.edu.au/style/images/fileicons/application_pdf.png" class="ep_doc_icon" border="0" /></a></td><td valign="top"><a href="http://eprints.utas.edu.au/2633/1/Athero_lax.pdf"><span class="ep_document_citation">PDF</span></a> - Full text restricted - Requires a PDF viewer<br />477Kb</td><td><form method="get" accept-charset="utf-8" action="http://eprints.utas.edu.au/cgi/request_doc"><input accept-charset="utf-8" value="3447" name="docid" type="hidden" /><div class=""><input value="Request a copy" name="_action_null" class="ep_form_action_button" onclick="return EPJS_button_pushed( '_action_null' )" type="submit" /> </div></form></td></tr></table><p style="margin-bottom: 1em" class="not_ep_block">Official URL: <a href="http://dx.doi.org/10.1071/BT99032">http://dx.doi.org/10.1071/BT99032</a></p><div class="not_ep_block"><h2>Abstract</h2><p style="padding-bottom: 16px; text-align: left; margin: 1em auto 0em auto">Random amplified polymorphic DNA (RAPD) and single-strand conformation polymorphism (SSCP)&#13;
  269. analyses were employed for investigating genetic relationships of three Athrotaxis D.Don species. Twenty-nine&#13;
  270. RAPD primers produced 103 polymorphic bands. Principal component analysis revealed the genomic differentiation&#13;
  271. among three Athrotaxis species. Mean genetic distance (mean d) between A. selaginoides D.Don and A. cupressoides&#13;
  272. D.Don was 0.89. Mean d values were reduced to 0.42/0.54 between A. laxifolia Hook. and A. selaginoides/A. cupressoides,&#13;
  273. respectively. Intraspecific mean d of A. selaginoides and A. cupressoides were, respectively, 0.03 and 0.11. These&#13;
  274. values indicated that A. laxifolia, which is regarded as a hybrid between A. selaginoides and A. cupressoides, is genetically&#13;
  275. intermediate between A. selaginoides and A. cupressoides. This genetic characteristic and previously reported morphological&#13;
  276. characteristics suggest the hybrid origins of A. laxifolia. The genomic composition of A. laxifolia was&#13;
  277. estimated by the number of bands specific to A. selaginoides or A. cupressoides in order to determine the genomic contribution&#13;
  278. of these two species to its proposed hybrid, A. laxifolia. All of the five individuals investigated herein possessed&#13;
  279. genomes derived almost evenly from A. selaginoides and A. cupressoides. Furthermore, the pollen donor of&#13;
  280. A. laxifolia was determined by SSCP analysis of the atpAgene on chloroplast DNA. Because all of the five A. laxifolia&#13;
  281. possessed the A. selaginoides-type chloroplast genome, A. laxifolia would be a hybrid of A. selaginoides as a paternal&#13;
  282. parent and A. cupressoides as a maternal parent.</p></div><table style="margin-bottom: 1em" cellpadding="3" class="not_ep_block" border="0"><tr><th valign="top" class="ep_row">Item Type:</th><td valign="top" class="ep_row">Article</td></tr><tr><th valign="top" class="ep_row">Subjects:</th><td valign="top" class="ep_row">UNSPECIFIED</td></tr><tr><th valign="top" class="ep_row">ID Code:</th><td valign="top" class="ep_row">2633</td></tr><tr><th valign="top" class="ep_row">Deposited By:</th><td valign="top" class="ep_row"><span class="ep_name_citation"><span class="person_name">Scholarly Publications Librarian</span></span></td></tr><tr><th valign="top" class="ep_row">Deposited On:</th><td valign="top" class="ep_row">05 Dec 2007 12:19</td></tr><tr><th valign="top" class="ep_row">Last Modified:</th><td valign="top" class="ep_row">09 Jan 2008 02:30</td></tr><tr><th valign="top" class="ep_row">ePrint Statistics:</th><td valign="top" class="ep_row"><a target="ePrintStats" href="/es/index.php?action=show_detail_eprint;id=2633;">View statistics for this ePrint</a></td></tr></table><p align="right">Repository Staff Only: <a href="http://eprints.utas.edu.au/cgi/users/home?screen=EPrint::View&amp;eprintid=2633">item control page</a></p>
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