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<title>UTas ePrints - HPG axis activity in juvenile blotched blue-tongued skinks, Tiliqua nigrolutea</title>
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<meta content="Edwards, Ashley" name="eprints.creators_name" />
<meta content="Jones, Susan M." name="eprints.creators_name" />
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<meta content="We are investigating seasonal sex- and age-related differences in the activity of the hypothalamic-pituitary-gonadal axis in the blotched blue-tongued lizard, Tiliqua nigrolutea as part of an overall study of the onset of sexual maturation and its subsequent annual regulation in a seasonally-breeding, viviparous reptile. Males of this species breed annually, while females exhibit a multiennial cycle; age at maturity is unknown. Juveniles at ages 1, 6, 12, 18, 24 and 30 months of age were injected with GnRH and we measured the resulting plasma sex steroid (testosterone (T) and oestradiol (E2)) concentrations, to examine the age of onset of HPG axis regulation: we plan to follow these juveniles over time until sexual maturity is reached. We found clear sex- and age-related differences in the production of T and E2 (as a measure of the activity of the HPG axis) from a relatively early age. We have used a number of techniques to confirm sex in these juveniles, and have correlated this with changes in T:E2 at ages 24 and 30 months. This longitudinal study of juveniles of (now) known sexes has allowed us to examine characteristics such as head width and body size (growth) for the onset of the sexual dimorphisms observed in adults. We have correlated these to endocrine profiles and other aspects of life history." name="eprints.abstract" />
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<meta content="We are investigating seasonal sex- and age-related differences in the activity of the hypothalamic-pituitary-gonadal axis in the blotched blue-tongued lizard, Tiliqua nigrolutea as part of an overall study of the onset of sexual maturation and its subsequent annual regulation in a seasonally-breeding, viviparous reptile. Males of this species breed annually, while females exhibit a multiennial cycle; age at maturity is unknown. Juveniles at ages 1, 6, 12, 18, 24 and 30 months of age were injected with GnRH and we measured the resulting plasma sex steroid (testosterone (T) and oestradiol (E2)) concentrations, to examine the age of onset of HPG axis regulation: we plan to follow these juveniles over time until sexual maturity is reached. We found clear sex- and age-related differences in the production of T and E2 (as a measure of the activity of the HPG axis) from a relatively early age. We have used a number of techniques to confirm sex in these juveniles, and have correlated this with changes in T:E2 at ages 24 and 30 months. This longitudinal study of juveniles of (now) known sexes has allowed us to examine characteristics such as head width and body size (growth) for the onset of the sexual dimorphisms observed in adults. We have correlated these to endocrine profiles and other aspects of life history." name="DC.description" />
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<h1 class="ep_tm_pagetitle">HPG axis activity in juvenile blotched blue-tongued skinks, Tiliqua nigrolutea</h1>
<p style="margin-bottom: 1em" class="not_ep_block"><span class="person_name">Edwards, Ashley</span> and <span class="person_name">Jones, Susan M.</span> (2006) <xhtml:em>HPG axis activity in juvenile blotched blue-tongued skinks, Tiliqua nigrolutea.</xhtml:em> ANZSCPB 2006. (Unpublished)</p><p style="margin-bottom: 1em" class="not_ep_block"></p><table style="margin-bottom: 1em" class="not_ep_block"><tr><td valign="top" style="text-align:center"><a onmouseover="EPJS_ShowPreview( event, 'doc_preview_658' );" href="http://eprints.utas.edu.au/647/1/Edwards_and_Jones.pdf" onmouseout="EPJS_HidePreview( event, 'doc_preview_658' );"><img alt="[img]" src="http://eprints.utas.edu.au/style/images/fileicons/application_pdf.png" class="ep_doc_icon" border="0" /></a><div class="ep_preview" id="doc_preview_658"><table><tr><td><img alt="" src="http://eprints.utas.edu.au/647/thumbnails/1/preview.png" class="ep_preview_image" border="0" /><div class="ep_preview_title">Preview</div></td></tr></table></div></td><td valign="top"><a href="http://eprints.utas.edu.au/647/1/Edwards_and_Jones.pdf"><span class="ep_document_citation">PDF</span></a> - Requires a PDF viewer<br />11Kb</td></tr></table><div class="not_ep_block"><h2>Abstract</h2><p style="padding-bottom: 16px; text-align: left; margin: 1em auto 0em auto">We are investigating seasonal sex- and age-related differences in the activity of the hypothalamic-pituitary-gonadal axis in the blotched blue-tongued lizard, Tiliqua nigrolutea as part of an overall study of the onset of sexual maturation and its subsequent annual regulation in a seasonally-breeding, viviparous reptile. Males of this species breed annually, while females exhibit a multiennial cycle; age at maturity is unknown. Juveniles at ages 1, 6, 12, 18, 24 and 30 months of age were injected with GnRH and we measured the resulting plasma sex steroid (testosterone (T) and oestradiol (E2)) concentrations, to examine the age of onset of HPG axis regulation: we plan to follow these juveniles over time until sexual maturity is reached. We found clear sex- and age-related differences in the production of T and E2 (as a measure of the activity of the HPG axis) from a relatively early age. We have used a number of techniques to confirm sex in these juveniles, and have correlated this with changes in T:E2 at ages 24 and 30 months. This longitudinal study of juveniles of (now) known sexes has allowed us to examine characteristics such as head width and body size (growth) for the onset of the sexual dimorphisms observed in adults. We have correlated these to endocrine profiles and other aspects of life history.</p></div><table style="margin-bottom: 1em" cellpadding="3" class="not_ep_block" border="0"><tr><th valign="top" class="ep_row">Item Type:</th><td valign="top" class="ep_row">Other</td></tr><tr><th valign="top" class="ep_row">Additional Information:</th><td valign="top" class="ep_row">Conference abstract Australia and New Zealand Society for Comparative Physiologists and Biochemists, Brisbane 2006</td></tr><tr><th valign="top" class="ep_row">Subjects:</th><td valign="top" class="ep_row"><a href="http://eprints.utas.edu.au/view/subjects/270604.html">270000 Biological Sciences > 270600 Physiology > 270604 Comparative Physiology</a><br /><a href="http://eprints.utas.edu.au/view/subjects/270603.html">270000 Biological Sciences > 270600 Physiology > 270603 Animal Physiology - Systems</a></td></tr><tr><th valign="top" class="ep_row">ID Code:</th><td valign="top" class="ep_row">647</td></tr><tr><th valign="top" class="ep_row">Deposited By:</th><td valign="top" class="ep_row"><span class="ep_name_citation"><span class="person_name">Dr Ashley Edwards</span></span></td></tr><tr><th valign="top" class="ep_row">Deposited On:</th><td valign="top" class="ep_row">19 Jan 2007</td></tr><tr><th valign="top" class="ep_row">Last Modified:</th><td valign="top" class="ep_row">09 Jan 2008 02:30</td></tr><tr><th valign="top" class="ep_row">ePrint Statistics:</th><td valign="top" class="ep_row"><a target="ePrintStats" href="/es/index.php?action=show_detail_eprint;id=647;">View statistics for this ePrint</a></td></tr></table><p align="right">Repository Staff Only: <a href="http://eprints.utas.edu.au/cgi/users/home?screen=EPrint::View&eprintid=647">item control page</a></p>
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